Rh4DG285400

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010445
Physical Location & Seq
Reverse (-)
50655871 .. 50657129
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG285400.1

Sequence Viewer

Length: 576 bp
ATGAAGAAGGCTTCAGTTTTCTTGAAGCAGATAATCACCTTGCTGAGCTCGATAGCCAAAGCCAAGACAATGGCGATCAAGAGCAAAACCAGCGCGGCCAAGGCTCGCCTCGTAATGTTCTCTTTGCTGAAGAACAAGAAGGTCTTGATGGATACTGTCTCCCACAAGTTTCATAACCTCCTCGGCCACCACGACAAAGACAGCAACCAAGGGGATGGCGATGGAGACGAGAGCCGGGCCATTGTGCTCTACAATGCCATTGCCAACGAGTACTCGCATGCAGCTGGCTCGTGCCGCCTGCACACCATTGAAGACGAGGAGGAGGATGACAATGACAAGTACCCGGATCTGAGGCACTGCCTATTCGACGAAGACGAAAACTTTGATGACATTAAGGCCGGTGTGTCTGTGGTTGATATTGTTAAGAATTCGAGAGTGGAAGGAGAAGATTTCAAGCTGGAAGACGAGATAGATCAAGTTGCGGACTTGTTCATTACAAGGTTTCATAAACAAATCCGCTTGCAGAAGCTTGAGTCCTTCAAGAGGTACAAAGACATGCTCGAGAGAAGCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

191

Amino Acids

21.82

Weight (kDa)

5.91

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 154 - 189 1.3e-15 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 4 cut(s) 95, 295, 482, 517
AclWI GGATC 1 cut(s) 354
AcoI YGGCCR 2 cut(s) 96, 184
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 3 cut(s) 272, 341, 548
AfiI CCNNNNNNNGG 1 cut(s) 543
AgsI TTSAA 4 cut(s) 25, 311, 454, 541
AluBI AGCT 4 cut(s) 48, 284, 457, 529
AluI AGCT 4 cut(s) 48, 284, 457, 529
Alw21I GWGCWC 2 cut(s) 50, 249
Alw26I GTCTC 2 cut(s) 163, 219
AlwI GGATC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 4 cut(s) 96, 184, 237, 396
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 237
AsuC2I CCSGG 2 cut(s) 236, 344
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 560
BanII GRGCYC 1 cut(s) 50
BauI CACGAG 1 cut(s) 289
BbsI GAAGAC 3 cut(s) 318, 378, 468
Bbv12I GWGCWC 2 cut(s) 50, 249
BbvI GCAGC 1 cut(s) 293
BccI CCATC 3 cut(s) 142, 209, 215
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciVI GTATCC 1 cut(s) 145
BcnI CCSGG 2 cut(s) 236, 344
BcoDI GTCTC 2 cut(s) 163, 219
BfaI CTAG 1 cut(s) 574
BfuI GTATCC 1 cut(s) 145
BisI GCNGC 3 cut(s) 96, 282, 295
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 3 cut(s) 97, 283, 296
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 2 cut(s) 236, 344
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 237
BmrFI CCNGG 2 cut(s) 236, 344
BpiI GAAGAC 3 cut(s) 318, 378, 468
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 551
BpuMI CCSGG 2 cut(s) 236, 344
BsaJI CCNNGG 3 cut(s) 99, 181, 208
Bsc4I CCNNNNNNNGG 1 cut(s) 543
Bse118I RCCGGY 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 99, 181, 208
BseGI GGATG 2 cut(s) 220, 331
BseLI CCNNNNNNNGG 1 cut(s) 543
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 35, 341
BseRI GAGGAG 3 cut(s) 170, 332, 335
BseXI GCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 4 cut(s) 98, 186, 239, 398
BsiHKAI GWGCWC 2 cut(s) 50, 249
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 3 cut(s) 235, 344, 399
BslI CCNNNNNNNGG 1 cut(s) 543
BsmAI GTCTC 2 cut(s) 163, 219
BsmBI CGTCTC 1 cut(s) 219
BsnI GGCC 4 cut(s) 98, 186, 239, 398
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 50, 249
Bsp143I GATC 3 cut(s) 75, 346, 472
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 4 cut(s) 95, 295, 482, 517
BspANI GGCC 4 cut(s) 98, 186, 239, 398
BspCNI CTCAG 2 cut(s) 36, 342
BspFNI CGCG 1 cut(s) 95
BspPI GGATC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 99, 181, 208
BssMI GATC 3 cut(s) 75, 346, 472
BssSI CACGAG 1 cut(s) 289
BssT1I CCWWGG 2 cut(s) 99, 208
Bst2BI CACGAG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 157
BstC8I GCNNGC 5 cut(s) 106, 279, 286, 299, 521
BstDEI CTNAG 2 cut(s) 44, 350
BstENI CCTNNNNNAGG 1 cut(s) 541
BstF5I GGATG 2 cut(s) 220, 331
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 3 cut(s) 78, 349, 475
BstMAI GTCTC 2 cut(s) 163, 219
BstMBI GATC 3 cut(s) 75, 346, 472
BstMWI GCNNNNNNNGC 3 cut(s) 90, 101, 294
BstNSI RCATGY 2 cut(s) 281, 559
BstSCI CCNGG 2 cut(s) 234, 342
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 293
BstV2I GAAGAC 3 cut(s) 318, 378, 468
BstX2I RGATCY 1 cut(s) 346
BstXI CCANNNNNNTGG 2 cut(s) 70, 215
BstYI RGATCY 1 cut(s) 346
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 4 cut(s) 98, 186, 239, 398
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 220, 331
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 355
Cac8I GCNNGC 5 cut(s) 106, 279, 286, 299, 521
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 1 cut(s) 237
Csp6I GTAC 3 cut(s) 271, 340, 547
CviAII CATG 2 cut(s) 278, 556
CviQI GTAC 3 cut(s) 271, 340, 547
DdeI CTNAG 2 cut(s) 44, 350
DpnI GATC 3 cut(s) 77, 348, 474
DpnII GATC 3 cut(s) 75, 346, 472
EaeI YGGCCR 2 cut(s) 96, 184
Ecl136II GAGCTC 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 99, 208
Eco24I GRGCYC 1 cut(s) 50
Eco53kI GAGCTC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 48
EcoNI CCTNNNNNAGG 1 cut(s) 541
EcoRI GAATTC 1 cut(s) 427
EcoT14I CCWWGG 2 cut(s) 99, 208
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 2 cut(s) 99, 208
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 2 cut(s) 281, 559
FaiI YATR 4 cut(s) 174, 279, 507, 557
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FatI CATG 2 cut(s) 277, 555
Fnu4HI GCNGC 3 cut(s) 96, 282, 295
FokI GGATG 2 cut(s) 227, 338
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 3 cut(s) 96, 282, 295
FspBI CTAG 1 cut(s) 574
GlaI GCGC 1 cut(s) 94
GluI GCNGC 3 cut(s) 96, 282, 295
HaeIII GGCC 4 cut(s) 98, 186, 239, 398
HapII CCGG 3 cut(s) 235, 344, 399
HhaI GCGC 1 cut(s) 95
Hin1II CATG 2 cut(s) 281, 559
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 1 cut(s) 533
HpaII CCGG 3 cut(s) 235, 344, 399
HphI GGTGA 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 6 cut(s) 22, 79, 145, 432, 541, 562
Hpy99I CGWCG 1 cut(s) 371
HpyAV CCTTC 3 cut(s) 133, 434, 547
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 3 cut(s) 281, 301, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 101, 294
HpyF3I CTNAG 2 cut(s) 44, 350
Hsp92II CATG 2 cut(s) 281, 559
HspAI GCGC 1 cut(s) 93
Kzo9I GATC 3 cut(s) 75, 346, 472
LpnPI CCDG 7 cut(s) 103, 248, 270, 311, 357, 412, 443
Lsp1109I GCAGC 1 cut(s) 293
MaeI CTAG 1 cut(s) 574
MalI GATC 3 cut(s) 77, 348, 474
MboI GATC 3 cut(s) 75, 346, 472
MboII GAAGA 6 cut(s) 16, 142, 323, 383, 458, 473
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 50, 249
MluCI AATT 1 cut(s) 427
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 8 cut(s) 119, 188, 191, 310, 313, 316, 345, 537
MseI TTAA 2 cut(s) 393, 423
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 3 cut(s) 235, 344, 399
MspR9I CCNGG 2 cut(s) 236, 344
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 3 cut(s) 90, 101, 294
NciI CCSGG 2 cut(s) 236, 344
NdeII GATC 3 cut(s) 75, 346, 472
NlaIII CATG 2 cut(s) 281, 559
NmeAIII GCCGAG 1 cut(s) 162
NspI RCATGY 2 cut(s) 281, 559
PaeI GCATGC 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 560
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 372
PkrI GCNGC 3 cut(s) 97, 283, 296
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 50
PspPI GGNCC 1 cut(s) 237
PsuI RGATCY 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 3 cut(s) 272, 341, 548
RsaNI GTAC 3 cut(s) 271, 340, 547
SacI GAGCTC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 393, 423
SatI GCNGC 3 cut(s) 96, 282, 295
Sau3AI GATC 3 cut(s) 75, 346, 472
Sau96I GGNCC 1 cut(s) 237
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 2 cut(s) 236, 344
SduI GDGCHC 2 cut(s) 50, 249
Sfr274I CTCGAG 1 cut(s) 560
SlaI CTCGAG 1 cut(s) 560
SmlI CTYRAG 2 cut(s) 530, 560
SmoI CTYRAG 2 cut(s) 530, 560
SphI GCATGC 1 cut(s) 281
Sse9I AATT 1 cut(s) 427
SsiI CCGC 4 cut(s) 95, 295, 482, 517
SspMI CTAG 1 cut(s) 574
SstI GAGCTC 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 234, 342
StyI CCWWGG 2 cut(s) 99, 208
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 4 cut(s) 50, 366, 431, 561
TasI AATT 1 cut(s) 427
TatI WGTACW 1 cut(s) 270
TauI GCSGC 2 cut(s) 98, 297
Tru1I TTAA 2 cut(s) 393, 423
Tru9I TTAA 2 cut(s) 393, 423
TscAI CASTG 1 cut(s) 362
TseI GCWGC 1 cut(s) 281
TspDTI ATGAA 4 cut(s) 17, 161, 481, 494
TspRI CASTG 1 cut(s) 362
XagI CCTNNNNNAGG 1 cut(s) 541
XapI RAATTY 1 cut(s) 427
XceI RCATGY 2 cut(s) 281, 559
XhoI CTCGAG 1 cut(s) 560
XspI CTAG 1 cut(s) 574
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.