FvH4_4g24100

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003470
Physical Location & Seq
Reverse (-)
26385673 .. 26392167
6495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24100.t3

Sequence Viewer

Length: 3084 bp
ATGGTGCTTGGGATAAGAGGTAAGAGCAGGAAGAGTGCTGCGGTTGAGGTGGATTATGTTATACATGTGCTGGAAATTAAACCGTGGCCCTCGTCACAGGCTTTGAAATCGGTTCAGTCTGTGTTTCTTCAGTGGGAGAATGGGGATCAGGCGTCTGGATCGTTCTTTCGTAACGTTGGAGATGAGAGGATTGAGTTCGGGGAGTCTTTTAGGCTGCCGGTTGTTTTGTACAAGGAGAAGTCGAGGAAAAGCTCCGCGAGTGATAGTTTCCAGAAGAATATCTTGGAGTTTTACTTGTCTGAACCTCGTAAGGACAAGGCGGCTAAAGGTCATGGTTTGGGATCGGCTGTAATAAATCTTGCAGACTACGCGAATGCAGCTGAGACCACGAGTGTGAGTGTTCCGCTGAGCTTGAAGAAGAGTTCTAAGAGTTCAGCTCAGCCGGTTCTTTATGTTAGTGTTCAGCCTTGTGGTAAAGATGGCTGCAACTTGTCCAAACAAGTGTCGCTGGACAATAATGAAAATTATGGAAGTACGTCTGTTTCAGGGTCTCTGAATGAGGTGGATGGCGAGATTGATTCTTTCACTGATGATGATGGTGATGATCGTTCTTCACATTCATCACGTACTGTGACCTCTTCCGCTTTTGAGGCACCAGTCAGTTCATCACCAAGTGCTGATAAGAATGCATCAGAATCAACGATTGATAACACTAGAAGGACTTATGGGGAACCAACCGTACATTCTGTACCAGCTCCTGCAAGCACTGTTATGAATCAAGTCGCTAATGCGTTGAAACATCAAAGTGGAAGTTCATCTCCATTGTCGTCAATAGGCTCTTCCTCCCCTCAGAAACCTGCTTATGATTATATTTCTTTGCCTCACCGTCCAAGGGACAGCTCAGTGCCAAGCTTAAAAAAATCATTGACTCAGTCTGTTCAATCTTCTTCCTCTTCTGGCTATCAGGATGATCATCAAGAGTTTGGTGATTATAATTTTAAGACCAACAGAATTCACAAGAGTCTTACTAGCAGAGGTGTCAGAATGCAAGAAAATGCACAAGAAGCTACTAAGGGAAAAAATGTGAGCAACCATGCTTCTGAAGGTACAACCTCAAGTATGTCTGTGCAGCAGGATACAAACTCATTATCTGCAAGCTACGTAGATTTAGAATCCCCCCGAGAGGATGACCATCTCGTGAAGGTGAACGACTATTCTTTTGATGGCAAATTAGCTTCCAGATTACAAGCTGGTACCAGAAAGCCAGTCACTATAAAGAGTGAAACTTTTACAGTCAGCAACAATGTTGGAGCATGGGAAAACAAGGTTAAAAGTACTGAAGTAAAGCAAGTAGAGTCTTTGGAACCTTCTGTATCAGCTAAGAACAATGGTCTGTTAAGAAAACACGAGTTGAAAAAGAAGTCAAAGGAAGCTGAGACTCCTGAGGATGGCCATGTTGGTGGTATCATTAGTGCAAAGAGTAAAAGAGAAGAAACAACAACCAGCTCTTCTGATAGTAAAAATGAGTTGGAGTCTACAATTGAAATGCTTAAGGACGAGTTGAGAGAAGCTGCTGCTGTTGAGGTTGCCCTTTACTCCATTGCTGCTGAGCATGGTGGTAACGCGAATAAGATCCATGCTCCTGCTCGTCGACTTTCTAGATTCTATATTCATGCATGCAAAATGGGATCTCAAGCTAAGAAGGCAAATGCAGCAAGAGCTGCTGTTACCGGATTAATTTTGGTTTCTAAAGCTTGTGGAAATGATGTTCCAAGGTTGACTTTCTGGTTGTCGAACTCAATTGTGTTGAGGGCTGTTGTCAGTCAGGGCTTAGTGAAAGCTCAAGTTTCAAATGGTAAACGGACTACAATTAAAGGCGGTGGACAGCATTTAGCGGGTGGGCGTTTATCAGAAAAGGATAGAATTCGTACTCACAAGGATGAGAAAAATAACATTCTTAAAAGTACTGATAATTGGGAAGATCCGCATATATTTATGGTTGCTTTGGAAAAGTTTGAAGCTTGGATATTCTCCCGGATAGTTGAGTCTGTATGGTGGCAGAATATTACTCCACATATGCAGCCTGCAGCTGCGAAAGGCTCAAGCACAAGGAAAGGAAATGGAAGAAAGAATGGTTTGGGTGATCAGGAGCAGGGTAATTTCTCTATTGAGCTGTGGACGAAGGCTTTCAAAGGTGCCTGTGAAAGGCTTTGTCCTGTTCGAGCTGGCGGCCATGAGTGTGGCTGCTTGCCTTTGCTAGCGCGTTTGGTCATGGAGCAGTTGGTGAATAGGTTGGATGTGGCTATGTTTAATGCTATTCTTCGTGAAAATGCTGAAGAGATGCCTACAGATCCCGTATCTGACCCTATAAGTGATTCCAAGGTTCTTCCAATTCCAGCTGGAAAATCAAGTTTTGGGGCAGGTGCACAGCTAAAAAATGTGATTGGAAGCTGGTCCAGATGGCTGACTGATCTATTTGATATGGATGATACTGATGTTCCAGACAATGAGGATGAACTCACTGATCACAAGGGGCAGGAGTGTGAAACAACCTTCAAAGCTTTCCGTCTTCTTAATGCTTTGAGTGATCTCATGATGCTTCCATCTGAAATGCTAGCAGATAAATCCACCAGAGAAGAGGTATGCCCTACATTCGGTGCATCATTGATCAAGAGGGTCCTATACAATTTTGTTACAGATGAGTTTTGCCCAGATCCAATTCCCGAAGCAGTTTTTGAGGCTCTTGATGATGAGGAGAATCTGGAAGCTGAAACAGAATCTGTTACAAGTTTCCCATTTATTGCAAATCCCACATTCTATTCACCACCTCCAGCTACTGCTTCTCTAATAGGCATTGCAGGAGAGGTGGGGAGCCCAGCTCTGAAGAGTGGGTCATCAGTGCTTAAGAAGTCATATACGAGTGATGATGAGCTTGATGAGTTGGATTCACCAATGACTTCAATTGTAGAAAATTCGCTAGTTTCCCCCAAGTCATTGACAGCCAACCCAATGCTGAAGTGGAAGGGTGGTCGCAAAGTTGTCCGATATCAACTCCTCCGTCAGGTATGGAAGGACAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1028

Amino Acids

111.99

Weight (kDa)

6.05

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 12 - 160 6e-16 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AarI CACCTGC 1 cut(s) 2411
Acc36I ACCTGC 2 cut(s) 865, 2411
Acc65I GGTACC 1 cut(s) 1253
AccB1I GGYRCC 3 cut(s) 652, 1253, 2195
AccI GTMKAC 2 cut(s) 1535, 1651
AccII CGCG 4 cut(s) 257, 371, 1625, 2263
AciI CCGC 9 cut(s) 41, 255, 320, 404, 642, 1878, 1895, 1985, 2229
AclI AACGTT 1 cut(s) 174
AclWI GGATC 8 cut(s) 153, 166, 349, 1627, 1696, 1976, 2345, 2708
AcoI YGGCCR 2 cut(s) 1450, 2230
AcsI RAATTY 3 cut(s) 1011, 1923, 2971
AcuI CTGAAG 6 cut(s) 113, 1122, 1359, 2355, 2903, 3035
AcyI GRCGYC 1 cut(s) 152
AdeI CACNNNGTG 1 cut(s) 674
AfiI CCNNNNNNNGG 6 cut(s) 892, 1183, 1448, 2205, 2654, 3061
AflII CTTAAG 2 cut(s) 1550, 2903
AflIII ACRYGT 1 cut(s) 64
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 727, 759
AleI CACNNNNGTG 1 cut(s) 392
AloI GAACNNNNNNTCC 2 cut(s) 1752, 1784
Alw21I GWGCWC 1 cut(s) 2428
Alw26I GTCTC 3 cut(s) 377, 555, 1430
Alw44I GTGCAC 1 cut(s) 2424
AlwI GGATC 8 cut(s) 153, 166, 349, 1627, 1696, 1976, 2345, 2708
AlwNI CAGNNNCTG 3 cut(s) 758, 2780, 2837
Ama87I CYCGRG 1 cut(s) 1179
AoxI GGCC 3 cut(s) 86, 1450, 2230
ApaLI GTGCAC 1 cut(s) 2424
ApoI RAATTY 3 cut(s) 1011, 1923, 2971
ArsI GACNNNNNNTTYG 2 cut(s) 1202, 1234
AseI ATTAAT 1 cut(s) 1736
Asp700I GAANNNNTTC 1 cut(s) 2186
Asp718I GGTACC 1 cut(s) 1253
AspLEI GCGC 1 cut(s) 2263
AspS9I GGNCC 3 cut(s) 87, 2454, 2677
AsuC2I CCSGG 1 cut(s) 2035
AsuHPI GGTGA 9 cut(s) 611, 660, 875, 998, 1216, 2153, 2296, 2814, 2940
AsuNHI GCTAGC 2 cut(s) 2257, 2614
AvaI CYCGRG 1 cut(s) 1179
AvaII GGWCC 2 cut(s) 2454, 2677
AxyI CCTNAGG 1 cut(s) 1443
BaeGI GKGCMC 1 cut(s) 2428
BaeI ACNNNNGTAYC 2 cut(s) 2481, 2514
BalI TGGCCA 1 cut(s) 1452
BanI GGYRCC 3 cut(s) 652, 1253, 2195
BanII GRGCYC 1 cut(s) 2876
BauI CACGAG 3 cut(s) 388, 1196, 1406
BbsI GAAGAC 1 cut(s) 2561
Bbv12I GWGCWC 1 cut(s) 2428
BccI CCATC 8 cut(s) 473, 560, 590, 1200, 1217, 1442, 2454, 2611
BciVI GTATCC 1 cut(s) 1129
BclI TGATCA 4 cut(s) 970, 2143, 2524, 2667
BcnI CCSGG 1 cut(s) 2035
BcoDI GTCTC 3 cut(s) 377, 555, 1430
BfaI CTAG 6 cut(s) 714, 1029, 1659, 2258, 2615, 2978
BfmI CTRYAG 2 cut(s) 2085, 2346
BfrI CTTAAG 2 cut(s) 1550, 2903
BfuAI ACCTGC 2 cut(s) 865, 2411
BfuI GTATCC 1 cut(s) 1129
BlpI GCTNAGC 3 cut(s) 407, 438, 1608
BmcAI AGTACT 2 cut(s) 1336, 1966
Bme1390I CCNGG 1 cut(s) 2035
Bme18I GGWCC 2 cut(s) 2454, 2677
BmeT110I CYCGRG 1 cut(s) 1179
BmgT120I GGNCC 3 cut(s) 87, 2454, 2677
BmiI GGNNCC 7 cut(s) 654, 732, 1255, 1365, 2197, 2678, 2873
BmrFI CCNGG 1 cut(s) 2035
BmsI GCATC 4 cut(s) 698, 2331, 2586, 2669
BmtI GCTAGC 2 cut(s) 2261, 2618
BpiI GAAGAC 1 cut(s) 2561
BplI GAGNNNNNCTC 4 cut(s) 421, 453, 2863, 2895
BpmI CTGGAG 1 cut(s) 2814
Bpu1102I GCTNAGC 3 cut(s) 407, 438, 1608
BpuEI CTTGAG 4 cut(s) 1099, 1677, 1827, 2086
BpuMI CCSGG 1 cut(s) 2035
BsaAI YACGTR 2 cut(s) 626, 1162
BsaBI GATNNNNATC 2 cut(s) 972, 1191
BsaHI GRCGYC 1 cut(s) 152
BsaI GGTCTC 2 cut(s) 377, 555
BsaJI CCNNGG 4 cut(s) 83, 890, 1772, 2379
BsaWI WCCGGW 1 cut(s) 1730
Bsc4I CCNNNNNNNGG 6 cut(s) 892, 1183, 1448, 2205, 2654, 3061
Bse118I RCCGGY 2 cut(s) 217, 442
Bse1I ACTGG 2 cut(s) 656, 1265
Bse21I CCTNAGG 1 cut(s) 1443
Bse3DI GCAATG 2 cut(s) 1599, 2853
Bse8I GATNNNNATC 2 cut(s) 972, 1191
BseDI CCNNGG 4 cut(s) 83, 890, 1772, 2379
BseGI GGATG 8 cut(s) 571, 973, 1192, 1453, 1945, 2302, 2491, 2518
BseJI GATNNNNATC 2 cut(s) 972, 1191
BseLI CCNNNNNNNGG 6 cut(s) 892, 1183, 1448, 2205, 2654, 3061
BseMI GCAATG 2 cut(s) 1599, 2853
BseMII CTCAG 9 cut(s) 372, 398, 452, 863, 915, 944, 1425, 1434, 1599
BseNI ACTGG 2 cut(s) 656, 1265
BseRI GAGGAG 2 cut(s) 2768, 3044
BseSI GKGCMC 1 cut(s) 2428
BseYI CCCAGC 1 cut(s) 2875
BsgI GTGCAG 1 cut(s) 1148
Bsh1236I CGCG 4 cut(s) 257, 371, 1625, 2263
BshFI GGCC 3 cut(s) 88, 1452, 2232
BshNI GGYRCC 3 cut(s) 652, 1253, 2195
BsiHKAI GWGCWC 1 cut(s) 2428
BsiHKCI CYCGRG 1 cut(s) 1179
BsiSI CCGG 4 cut(s) 218, 443, 1731, 2035
BslFI GGGAC 1 cut(s) 908
BslI CCNNNNNNNGG 6 cut(s) 892, 1183, 1448, 2205, 2654, 3061
BsmAI GTCTC 3 cut(s) 377, 555, 1430
BsmFI GGGAC 1 cut(s) 908
BsmI GAATGC 3 cut(s) 379, 691, 1050
BsnI GGCC 3 cut(s) 88, 1452, 2232
Bso31I GGTCTC 2 cut(s) 377, 555
BsoBI CYCGRG 1 cut(s) 1179
Bsp1286I GDGCHC 2 cut(s) 2428, 2876
Bsp1407I TGTACA 1 cut(s) 228
Bsp1720I GCTNAGC 3 cut(s) 407, 438, 1608
BspACI CCGC 9 cut(s) 41, 255, 320, 404, 642, 1878, 1895, 1985, 2229
BspANI GGCC 3 cut(s) 88, 1452, 2232
BspCNI CTCAG 9 cut(s) 373, 399, 451, 862, 914, 943, 1426, 1435, 1600
BspFNI CGCG 4 cut(s) 257, 371, 1625, 2263
BspHI TCATGA 1 cut(s) 2592
BspLI GGNNCC 7 cut(s) 654, 732, 1255, 1365, 2197, 2678, 2873
BspMAI CTGCAG 1 cut(s) 2089
BspMI ACCTGC 2 cut(s) 865, 2411
BspOI GCTAGC 2 cut(s) 2261, 2618
BspPI GGATC 8 cut(s) 153, 166, 349, 1627, 1696, 1976, 2345, 2708
BspQI GCTCTTC 2 cut(s) 844, 1513
BspT107I GGYRCC 3 cut(s) 652, 1253, 2195
BspTI CTTAAG 2 cut(s) 1550, 2903
BspTNI GGTCTC 2 cut(s) 377, 555
BsrDI GCAATG 2 cut(s) 1599, 2853
BsrFI RCCGGY 2 cut(s) 217, 442
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 2 cut(s) 656, 1265
BssAI RCCGGY 2 cut(s) 217, 442
BssECI CCNNGG 4 cut(s) 83, 890, 1772, 2379
BssNI GRCGYC 1 cut(s) 152
BssSI CACGAG 3 cut(s) 388, 1196, 1406
BssT1I CCWWGG 3 cut(s) 890, 1772, 2379
Bst2BI CACGAG 3 cut(s) 388, 1196, 1406
Bst4CI ACNGT 7 cut(s) 84, 631, 739, 769, 887, 1294, 3077
Bst6I CTCTTC 9 cut(s) 26, 413, 643, 844, 958, 1513, 2331, 2631, 2879
BstACI GRCGYC 1 cut(s) 152
BstAFI CTTAAG 2 cut(s) 1550, 2903
BstAPI GCANNNNNTGC 1 cut(s) 1721
BstAUI TGTACA 1 cut(s) 228
BstBAI YACGTR 2 cut(s) 626, 1162
BstC8I GCNNGC 8 cut(s) 763, 1156, 1678, 2085, 2227, 2249, 2259, 2616
BstDSI CCRYGG 1 cut(s) 83
BstENI CCTNNNNNAGG 2 cut(s) 2203, 3059
BstF5I GGATG 8 cut(s) 571, 973, 1192, 1453, 1945, 2302, 2491, 2518
BstFNI CGCG 4 cut(s) 257, 371, 1625, 2263
BstHHI GCGC 1 cut(s) 2263
BstMAI GTCTC 3 cut(s) 377, 555, 1430
BstMWI GCNNNNNNNGC 8 cut(s) 368, 377, 650, 1064, 1703, 1712, 1718, 1721
BstNSI RCATGY 2 cut(s) 68, 1680
BstSCI CCNGG 1 cut(s) 2033
BstSFI CTRYAG 2 cut(s) 2085, 2346
BstSLI GKGCMC 1 cut(s) 2428
BstSNI TACGTA 1 cut(s) 1162
BstUI CGCG 4 cut(s) 257, 371, 1625, 2263
BstV2I GAAGAC 1 cut(s) 2561
BstX2I RGATCY 5 cut(s) 1632, 1688, 1981, 2350, 2713
BstXI CCANNNNNNTGG 2 cut(s) 1460, 2240
BstYI RGATCY 5 cut(s) 1632, 1688, 1981, 2350, 2713
Bsu36I CCTNAGG 1 cut(s) 1443
BsuI GTATCC 1 cut(s) 1129
BsuRI GGCC 3 cut(s) 88, 1452, 2232
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 8 cut(s) 571, 973, 1192, 1453, 1945, 2302, 2491, 2518
BtsIMutI CAGTG 7 cut(s) 137, 585, 765, 909, 2520, 2904, 3082
BveI ACCTGC 2 cut(s) 865, 2411
Cac8I GCNNGC 8 cut(s) 763, 1156, 1678, 2085, 2227, 2249, 2259, 2616
CaiI CAGNNNCTG 3 cut(s) 758, 2780, 2837
CciI TCATGA 1 cut(s) 2592
CfoI GCGC 1 cut(s) 2263
Cfr10I RCCGGY 2 cut(s) 217, 442
Cfr13I GGNCC 3 cut(s) 87, 2454, 2677
CseI GACGC 1 cut(s) 141
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 2 cut(s) 1450, 2230
Eam1104I CTCTTC 9 cut(s) 26, 413, 643, 844, 958, 1513, 2331, 2631, 2879
EarI CTCTTC 9 cut(s) 26, 413, 643, 844, 958, 1513, 2331, 2631, 2879
Eco105I TACGTA 1 cut(s) 1162
Eco130I CCWWGG 3 cut(s) 890, 1772, 2379
Eco24I GRGCYC 1 cut(s) 2876
Eco31I GGTCTC 2 cut(s) 377, 555
Eco32I GATATC 1 cut(s) 3047
Eco47I GGWCC 2 cut(s) 2454, 2677
Eco57I CTGAAG 6 cut(s) 113, 1122, 1359, 2355, 2903, 3035
Eco81I CCTNAGG 1 cut(s) 1443
Eco88I CYCGRG 1 cut(s) 1179
EcoNI CCTNNNNNAGG 2 cut(s) 2203, 3059
EcoO109I RGGNCCY 1 cut(s) 2677
EcoRI GAATTC 2 cut(s) 1011, 1923
EcoRV GATATC 1 cut(s) 3047
EcoT14I CCWWGG 3 cut(s) 890, 1772, 2379
EcoT22I ATGCAT 2 cut(s) 691, 1678
EcoT38I GRGCYC 1 cut(s) 2876
ErhI CCWWGG 3 cut(s) 890, 1772, 2379
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 1765, 1797
FaqI GGGAC 1 cut(s) 908
FauI CCCGC 1 cut(s) 1888
FauNDI CATATG 1 cut(s) 2076
FbaI TGATCA 4 cut(s) 970, 2143, 2524, 2667
FblI GTMKAC 2 cut(s) 1535, 1651
FokI GGATG 8 cut(s) 578, 980, 1199, 1460, 1952, 2309, 2498, 2525
FriOI GRGCYC 1 cut(s) 2876
FspBI CTAG 6 cut(s) 714, 1029, 1659, 2258, 2615, 2978
GlaI GCGC 1 cut(s) 2262
GsaI CCCAGC 1 cut(s) 2879
GsuI CTGGAG 1 cut(s) 2814
HaeIII GGCC 3 cut(s) 88, 1452, 2232
HapII CCGG 4 cut(s) 218, 443, 1731, 2035
HgaI GACGC 1 cut(s) 141
HhaI GCGC 1 cut(s) 2263
Hin1I GRCGYC 1 cut(s) 152
Hin6I GCGC 1 cut(s) 2261
HinP1I GCGC 1 cut(s) 2261
HincII GTYRAC 2 cut(s) 1652, 1779
HindII GTYRAC 2 cut(s) 1652, 1779
HindIII AAGCTT 4 cut(s) 910, 1752, 2019, 2559
HpaII CCGG 4 cut(s) 218, 443, 1731, 2035
HphI GGTGA 9 cut(s) 611, 660, 875, 998, 1216, 2153, 2296, 2814, 2940
Hpy166II GTNNAC 8 cut(s) 1207, 1536, 1652, 1779, 1859, 1883, 2178, 2426
Hpy8I GTNNAC 8 cut(s) 1207, 1536, 1652, 1779, 1859, 1883, 2178, 2426
Hpy99I CGWCG 1 cut(s) 1653
HpyAV CCTTC 9 cut(s) 711, 1097, 1195, 1377, 1696, 2176, 2563, 3016, 3064
HpyCH4III ACNGT 7 cut(s) 84, 631, 739, 769, 887, 1294, 3077
HpyCH4IV ACGT 4 cut(s) 174, 536, 625, 1161
HpyF10VI GCNNNNNNNGC 8 cut(s) 368, 377, 650, 1064, 1703, 1712, 1718, 1721
HpySE526I ACGT 4 cut(s) 174, 536, 625, 1161
Hsp92I GRCGYC 1 cut(s) 152
HspAI GCGC 1 cut(s) 2261
KpnI GGTACC 1 cut(s) 1257
Ksp22I TGATCA 4 cut(s) 970, 2143, 2524, 2667
LguI GCTCTTC 2 cut(s) 844, 1513
LmnI GCTCC 7 cut(s) 257, 760, 1310, 1645, 2149, 2275, 2871
LweI GCATC 4 cut(s) 698, 2331, 2586, 2669
MaeI CTAG 6 cut(s) 714, 1029, 1659, 2258, 2615, 2978
MaeII ACGT 4 cut(s) 174, 536, 625, 1161
MaeIII GTNAC 8 cut(s) 93, 170, 631, 1267, 1619, 1726, 2692, 2782
MfeI CAATTG 3 cut(s) 1539, 1800, 2961
MflI RGATCY 5 cut(s) 1632, 1688, 1981, 2350, 2713
MhlI GDGCHC 2 cut(s) 2428, 2876
MlsI TGGCCA 1 cut(s) 1452
MluNI TGGCCA 1 cut(s) 1452
MlyI GAGTC 7 cut(s) 212, 922, 1030, 1364, 1432, 1541, 2054
MmeI TCCRAC 5 cut(s) 157, 1288, 1509, 2274, 2922
Mox20I TGGCCA 1 cut(s) 1452
Mph1103I ATGCAT 2 cut(s) 691, 1678
MroXI GAANNNNTTC 1 cut(s) 2186
MscI TGGCCA 1 cut(s) 1452
MslI CAYNNNNRTG 6 cut(s) 392, 770, 804, 1458, 1938, 2238
Msp20I TGGCCA 1 cut(s) 1452
MspA1I CMGCKG 4 cut(s) 380, 406, 2090, 2399
MspCI CTTAAG 2 cut(s) 1550, 2903
MspI CCGG 4 cut(s) 218, 443, 1731, 2035
MspR9I CCNGG 1 cut(s) 2035
MunI CAATTG 3 cut(s) 1539, 1800, 2961
Mva1269I GAATGC 3 cut(s) 379, 691, 1050
MvnI CGCG 4 cut(s) 257, 371, 1625, 2263
MwoI GCNNNNNNNGC 8 cut(s) 368, 377, 650, 1064, 1703, 1712, 1718, 1721
NciI CCSGG 1 cut(s) 2035
NdeI CATATG 1 cut(s) 2076
NheI GCTAGC 2 cut(s) 2257, 2614
NlaIV GGNNCC 7 cut(s) 654, 732, 1255, 1365, 2197, 2678, 2873
NmuCI GTSAC 3 cut(s) 93, 631, 1267
NsiI ATGCAT 2 cut(s) 691, 1678
NspI RCATGY 2 cut(s) 68, 1680
OliI CACNNNNGTG 1 cut(s) 392
PaeI GCATGC 1 cut(s) 1680
PagI TCATGA 1 cut(s) 2592
PaqCI CACCTGC 1 cut(s) 2411
PciI ACATGT 1 cut(s) 64
PciSI GCTCTTC 2 cut(s) 844, 1513
PctI GAATGC 3 cut(s) 379, 691, 1050
PdmI GAANNNNTTC 1 cut(s) 2186
PfeI GAWTC 9 cut(s) 578, 695, 775, 1172, 1662, 2375, 2758, 2777, 2945
PflFI GACNNNGTC 1 cut(s) 931
PfoI TCCNGGA 1 cut(s) 2033
PleI GAGTC 7 cut(s) 211, 922, 1029, 1363, 1432, 1540, 2053
PpsI GAGTC 7 cut(s) 211, 922, 1029, 1363, 1432, 1540, 2053
Ppu21I YACGTR 2 cut(s) 626, 1162
PpuMI RGGWCCY 1 cut(s) 2677
PscI ACATGT 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 1736
PsiI TTATAA 1 cut(s) 993
Psp1406I AACGTT 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 2677
PspFI CCCAGC 1 cut(s) 2875
PspN4I GGNNCC 7 cut(s) 654, 732, 1255, 1365, 2197, 2678, 2873
PspPI GGNCC 3 cut(s) 87, 2454, 2677
PspPPI RGGWCCY 1 cut(s) 2677
PsrI GAACNNNNNNTAC 2 cut(s) 723, 755
PstI CTGCAG 1 cut(s) 2089
PstNI CAGNNNCTG 3 cut(s) 758, 2780, 2837
PsuI RGATCY 5 cut(s) 1632, 1688, 1981, 2350, 2713
PsyI GACNNNGTC 1 cut(s) 931
PvuII CAGCTG 3 cut(s) 380, 2090, 2399
RseI CAYNNNNRTG 6 cut(s) 392, 770, 804, 1458, 1938, 2238
SalI GTCGAC 1 cut(s) 1650
SapI GCTCTTC 2 cut(s) 844, 1513
Sau96I GGNCC 3 cut(s) 87, 2454, 2677
ScaI AGTACT 2 cut(s) 1336, 1966
SchI GAGTC 7 cut(s) 212, 922, 1030, 1364, 1432, 1541, 2054
ScrFI CCNGG 1 cut(s) 2035
SduI GDGCHC 2 cut(s) 2428, 2876
SfaNI GCATC 4 cut(s) 698, 2331, 2586, 2669
SfcI CTRYAG 2 cut(s) 2085, 2346
SinI GGWCC 2 cut(s) 2454, 2677
SmiMI CAYNNNNRTG 6 cut(s) 392, 770, 804, 1458, 1938, 2238
SmlI CTYRAG 6 cut(s) 1114, 1550, 1692, 1842, 2101, 2903
SmoI CTYRAG 6 cut(s) 1114, 1550, 1692, 1842, 2101, 2903
SnaBI TACGTA 1 cut(s) 1162
SphI GCATGC 1 cut(s) 1680
SsiI CCGC 9 cut(s) 41, 255, 320, 404, 642, 1878, 1895, 1985, 2229
SspI AATATT 1 cut(s) 2065
SspMI CTAG 6 cut(s) 714, 1029, 1659, 2258, 2615, 2978
StyD4I CCNGG 1 cut(s) 2033
StyI CCWWGG 3 cut(s) 890, 1772, 2379
TaaI ACNGT 7 cut(s) 84, 631, 739, 769, 887, 1294, 3077
TaiI ACGT 4 cut(s) 177, 539, 628, 1164
TaqI TCGA 4 cut(s) 242, 1651, 1793, 2221
TatI WGTACW 3 cut(s) 228, 1334, 1964
TauI GCSGC 2 cut(s) 323, 2232
TfiI GAWTC 9 cut(s) 578, 695, 775, 1172, 1662, 2375, 2758, 2777, 2945
TscAI CASTG 7 cut(s) 137, 592, 772, 909, 2527, 2904, 3082
TseFI GTSAC 3 cut(s) 93, 631, 1267
Tsp45I GTSAC 3 cut(s) 93, 631, 1267
TspDTI ATGAA 7 cut(s) 534, 609, 654, 788, 804, 1661, 2529
TspGWI ACGGA 3 cut(s) 1876, 2555, 3047
TspRI CASTG 7 cut(s) 137, 592, 772, 909, 2527, 2904, 3082
Tth111I GACNNNGTC 1 cut(s) 931
Vha464I CTTAAG 2 cut(s) 1550, 2903
VneI GTGCAC 1 cut(s) 2424
VpaK11BI GGWCC 2 cut(s) 2454, 2677
VspI ATTAAT 1 cut(s) 1736
XagI CCTNNNNNAGG 2 cut(s) 2203, 3059
XapI RAATTY 3 cut(s) 1011, 1923, 2971
XbaI TCTAGA 1 cut(s) 1658
XceI RCATGY 2 cut(s) 68, 1680
XcmI CCANNNNNNNNNTGG 1 cut(s) 3015
XmiI GTMKAC 2 cut(s) 1535, 1651
XmnI GAANNNNTTC 1 cut(s) 2186
XspI CTAG 6 cut(s) 714, 1029, 1659, 2258, 2615, 2978
ZrmI AGTACT 2 cut(s) 1336, 1966
Zsp2I ATGCAT 2 cut(s) 691, 1678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.