MD13G1115500.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003470
Physical Location & Seq
Forward (+)
8372120 .. 8378642
6523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1115500.v1.1.491

Sequence Viewer

Length: 3057 bp
ATGGTTCTTGGGATCAGAGCAAAGAGCAGGAAGAGCGCGGCCGTTGAGGTTGATTATCTCATCCATGTGCAGGAGTTAAAGCCATGGCCCTCATCTCAGGCTTTGAAGTCTGTTCAATCTGTCCTGCTTCAATGGGAAAATGGTGATCAGGTTTCTGGATCGTGTATTTGCAATGTTGGTGATGGGAAGATCGAAATCGGCGAGTCTTTCACGCTTCCAGTCACTTTATATAAGGAGAAATCAAGAAAAAGTGCAGTTCGCGACTCTTACCTGAAGAACAACTTAGAGTTTTACTTGTATGAACCTCGAAAGGACAAGGGAGTTAAAGGTCAACTCTTGGGGTCAGCTGTGATCAATCTTGCTGACTATGGGATCATTGTGGAAACCCGAAATGTGAGCACTCCTCTGAACTGGAAGAAAAGTTTTAAGAGTTCATCTCAACCGGTTCTTTATGTTACTATTCAGCCATGTGGTAAATCTAGCTCCAGCTTGACACCGAAGAGTAGCTTGTCCAGAGAAGTGTCTCTGGAAAATGATGGAACTGAATCTGTTCCAGAGTCTGCGAATGATGGCAATGATGAGATTGCCTCTTTTACTGATGACGATGATGATGTTTCCTCGCGTTCATCACGTACTGTAAACTCCTCGGCTATTGAGACAACAGTCAGTTCATCACCTAGCGGTGAAAAGAATGGACTAGAATCAACAATCAGTAAAAGGACTTATGGGGAACCTGCTGTACAGTCAATGGCAGCAACTGCAAGCACAGGGGTAACTCCAGTGGCTAAAGCATTCAAACACCAAAATGGAAGTTCATCTCCCCCGTCATCAATAGGCTCTTCCTCCTTTCTGCTGAACTCTTCAAATGATCCTGCTTCCTTGCCTAACAGTTCAAAGGAGAGTTCAATGCCAATCCTTAAAAAATCACTCACTCACTCTGTTCATTCAGCGTCTTCATCCTTTGGTTATCAAGATAGCCATCAGGTATCTGGTTACCATAATTTTAAGGACAACAGAATTCACATAACTCCATCTAACAGGAGTGCCAGTATATATGAAAATGCTCAAAAACTTATCACGGGAAATGTTGTCAGTACCCATGCTGCTGAACGTGCATCCTCGAGTATGTCTATTCAGGAGGACACAAACTCCACATTTGCAAGCAATGCAGATTCACAAGCCCTTCGAGAGGATGGCCATCTCCTAATGGCGAATCAATATTCTTTTGATGACAAATTAGCTCCTAGATTTTCACAAGAGCCTACCAGAAAGCAAGTCAGGTTAAGGAGTGAAACTTTTACACCCAGCAGGAAAGTTGTTGTAGTGCAGGGAAGTAAGGCTAAAAGTAATGAACTAAAGCATGTAGAGTCTGTGCAGTTACCTTTAGTATCAGCTCAGAACAATAGACAGTCAAGCAATAATGAGTTTCTCAAAAAGTCAAAGGAAGCTGAAACTCCTGAAGATGCTTCTGTTCACGGTTGGATTAGCTCAACAAGTGAAAGTGAACAAAAAATAGTCAGCTTTTCAGATAGCAAAGTTGACTTGGAGTCCACAATTGAATTGCTCAAGGAAGAACTGAGAGAAGCTGCTGCTGTTGAGGTTGGCCTTTACTCTGTTGCCGCTGAGCATGGGAGCCACACTAATAAGATTCACGCTCCTGCTCGGCGACTTTCTAGGTTCTATTTTCATGCTTGCAAGACAAGCTCGCGAGCTAACAAGGCAAATGCAGCAAGAGCTGCTATCACTGGACTAATCTTGGTTTCTAAAGCATGCGGAAATGATGTTGCGAGGTTGACATTCTGGTTGTCAAATTCAATTGTGTTGAGAGCTATCATCAGCCAGACCTTGTCCTGCAATAAAATTAATGCTGAGGGGTTTTTGAAACCAAAGAATGGGTTTCCTCCTCACAAGGAAGAGAACAATAGCACTGTTGAAAGTTTCGATGATTGGGAAGATCCACAGATATTTATGGCAGCTTTGGAAAAGTTTGAAGGATGGATTTTCTCCCGAATTGTTGAGTCTGTATGGTGGCAGAATATGACTCCATATATGCAGTCAGCAGCTGCAAAGGGTTCAAGCTCAAGGAAAGCCAGTGGAAGGAGGCATGGTTTGGGTAGTCATGAGCAGGGTAACTTTTCTATGGAGCTGTGGAAAAAGGCTTTCAAAGATGCCTGTGAAAGGCTTTGTCCTGCAAGAGCTGGTGGACATGAGTGTGGCTGCTTGCCTCTGCTGGCTCGTTTGGTAATGGAGCAATTAGTGGATAGATTGGATGTGGCTATGTTCAATGCTATTCTTCGTGAAAATGCTGAAGAGATGCCCACAGATCCTGTGTCCGACCCCATAAGTGATTCTAAGGTTCTCCCTATTCCAGCTGGAAATTTGAGTTTTGGGGCTGGTGTACAGCTAAAAAATGCGATTGGAAGCTGGTCCAGATGGCTTACTGATCTATTTGGCATCGATGATACTGATGCTCCTGATGATAATACTGAACTCAGTGATCACAAGGGACAAGAGTGCGAAACATCCTTTAAAGCTTTCCGTCTACTTAATGCTTTGAGTGACCTCATGATGCTTCCATTGGATATGCTTGCAGATAAATCCACCCGGAAAGAGGTATGCCCTACATTTGGTGCATCATTGATCAAGAGGGTTCTTTACAATTTTGTTCCAGATGAGTTTTGCCCAGACCCAATTCCTGAAGCAGTTTTTGAGGCTCTTGATTATGAGGAGGATTTGGAAGCTGAAGCAGAGTCAGCTGCAAGTTTCCCATGCAACGCAAATCCTACAGTGTATTCACCACCTCCAGCTGCTTCACTACTAGGCATTATAGGAGATGTGGGAAGTCCAACTTTCTCTAGGAGTGGGTCATCAGTGCTTAAGAAATCTTATACAAGTGATGACGAGCTTGATGAGTTGGATTCACCGATGACTTCAATCATCATAGACAATTCTCCGTTTTCTCCCAGTCCATTGGCAGCCTCAACGCCGAAGTGGAAGGGTGGTCAGAAAGCTGTCAGGTATCAACTCCTCCGTGAAGTTTGGAAGGATAGCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1019

Amino Acids

111.13

Weight (kDa)

5.66

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 12 - 155 3.1e-13 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 742
AccB7I CCANNNNNTGG 1 cut(s) 1892
AccI GTMKAC 1 cut(s) 2542
AccII CGCG 4 cut(s) 38, 261, 622, 1708
AciI CCGC 4 cut(s) 38, 681, 1620, 1773
AclWI GGATC 6 cut(s) 20, 166, 380, 863, 1949, 2318
AcoI YGGCCR 2 cut(s) 39, 1195
AcsI RAATTY 3 cut(s) 1017, 1810, 2377
AcuI CTGAAG 5 cut(s) 293, 1479, 2328, 2718, 2763
AfaI GTAC 4 cut(s) 634, 741, 1096, 2400
AfiI CCNNNNNNNGG 7 cut(s) 70, 1189, 1892, 2097, 2178, 2611, 2627
AflII CTTAAG 1 cut(s) 2876
AgeI ACCGGT 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 1546
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 528, 650
AlwI GGATC 6 cut(s) 20, 166, 380, 863, 1949, 2318
AlwNI CAGNNNCTG 4 cut(s) 560, 758, 2063, 2327
Ama87I CYCGRG 1 cut(s) 1120
AoxI GGCC 4 cut(s) 39, 86, 1195, 1603
ApoI RAATTY 3 cut(s) 1017, 1810, 2377
AseI ATTAAT 1 cut(s) 1863
AsiGI ACCGGT 1 cut(s) 442
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 87, 2427
AsuC2I CCSGG 1 cut(s) 2605
AsuHPI GGTGA 6 cut(s) 155, 191, 666, 695, 2787, 2913
AvaI CYCGRG 1 cut(s) 1120
AvaII GGWCC 1 cut(s) 2427
BalI TGGCCA 1 cut(s) 1197
BbsI GAAGAC 1 cut(s) 945
Bbv12I GWGCWC 1 cut(s) 401
BbvCI CCTCAGC 1 cut(s) 1869
BccI CCATC 9 cut(s) 176, 530, 563, 987, 1039, 1187, 1206, 1989, 2427
BceAI ACGGC 1 cut(s) 26
BclI TGATCA 4 cut(s) 145, 351, 2497, 2640
BcnI CCSGG 1 cut(s) 2605
BcoDI GTCTC 2 cut(s) 528, 650
BfaI CTAG 7 cut(s) 480, 678, 698, 1245, 1674, 2819, 2856
BfmI CTRYAG 1 cut(s) 2784
BfrI CTTAAG 1 cut(s) 2876
BfuAI ACCTGC 1 cut(s) 742
BlpI GCTNAGC 1 cut(s) 1623
Bme1390I CCNGG 1 cut(s) 2605
Bme18I GGWCC 1 cut(s) 2427
BmeRI GACNNNNNGTC 1 cut(s) 1546
BmeT110I CYCGRG 1 cut(s) 1120
BmgT120I GGNCC 2 cut(s) 87, 2427
BmiI GGNNCC 2 cut(s) 732, 1634
BmrFI CCNGG 1 cut(s) 2605
BmrI ACTGGG 1 cut(s) 2958
BmsI GCATC 8 cut(s) 1124, 1453, 2158, 2304, 2458, 2463, 2559, 2642
BmuI ACTGGG 1 cut(s) 2958
BoxI GACNNNNGTC 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 945
BplI GAGNNNNNCTC 6 cut(s) 388, 420, 421, 453, 572, 604
BpmI CTGGAG 3 cut(s) 469, 762, 2787
Bpu10I CCTNAGC 1 cut(s) 1869
Bpu1102I GCTNAGC 1 cut(s) 1623
BpuEI CTTGAG 2 cut(s) 1550, 2065
BpuMI CCSGG 1 cut(s) 2605
Bsa29I ATCGAT 1 cut(s) 2457
BsaAI YACGTR 1 cut(s) 632
BsaBI GATNNNNATC 3 cut(s) 194, 978, 1197
BsaJI CCNNGG 2 cut(s) 83, 645
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 8 cut(s) 1033, 1063, 1133, 1163, 2092, 2122, 2455, 2485
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 1189, 1892, 2097, 2178, 2611, 2627
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 7 cut(s) 218, 416, 779, 1047, 1750, 2091, 2964
Bse3DI GCAATG 3 cut(s) 178, 580, 1171
Bse8I GATNNNNATC 3 cut(s) 194, 978, 1197
BseCI ATCGAT 1 cut(s) 2457
BseDI CCNNGG 2 cut(s) 83, 645
BseGI GGATG 7 cut(s) 60, 956, 1115, 1198, 2000, 2275, 2522
BseJI GATNNNNATC 3 cut(s) 194, 978, 1197
BseLI CCNNNNNNNGG 7 cut(s) 70, 1189, 1892, 2097, 2178, 2611, 2627
BseMI GCAATG 3 cut(s) 178, 580, 1171
BseMII CTCAG 6 cut(s) 110, 1409, 1568, 1614, 1860, 2506
BseNI ACTGG 7 cut(s) 218, 416, 779, 1047, 1750, 2091, 2964
BseRI GAGGAG 5 cut(s) 393, 634, 1893, 2741, 3017
BseX3I CGGCCG 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 1304
BsgI GTGCAG 4 cut(s) 89, 273, 1346, 1394
Bsh1236I CGCG 4 cut(s) 38, 261, 622, 1708
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 41, 88, 1197, 1605
BshTI ACCGGT 1 cut(s) 442
BshVI ATCGAT 1 cut(s) 2457
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 1120
BsiSI CCGG 2 cut(s) 443, 2605
BslFI GGGAC 1 cut(s) 2520
BslI CCNNNNNNNGG 7 cut(s) 70, 1189, 1892, 2097, 2178, 2611, 2627
BsmAI GTCTC 2 cut(s) 528, 650
BsmFI GGGAC 1 cut(s) 2520
BsmI GAATGC 1 cut(s) 791
BsnI GGCC 4 cut(s) 41, 88, 1197, 1605
BsoBI CYCGRG 1 cut(s) 1120
Bsp1286I GDGCHC 1 cut(s) 401
Bsp1407I TGTACA 2 cut(s) 739, 2398
Bsp1720I GCTNAGC 1 cut(s) 1623
Bsp19I CCATGG 1 cut(s) 83
Bsp68I TCGCGA 2 cut(s) 261, 1708
BspACI CCGC 4 cut(s) 38, 681, 1620, 1773
BspANI GGCC 4 cut(s) 41, 88, 1197, 1605
BspCNI CTCAG 6 cut(s) 109, 1408, 1569, 1615, 1861, 2505
BspDI ATCGAT 1 cut(s) 2457
BspFNI CGCG 4 cut(s) 38, 261, 622, 1708
BspHI TCATGA 2 cut(s) 2119, 2565
BspLI GGNNCC 2 cut(s) 732, 1634
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 6 cut(s) 20, 166, 380, 863, 1949, 2318
BspQI GCTCTTC 2 cut(s) 26, 844
BspTI CTTAAG 1 cut(s) 2876
BsrDI GCAATG 3 cut(s) 178, 580, 1171
BsrFI RCCGGY 1 cut(s) 442
BsrGI TGTACA 2 cut(s) 739, 2398
BsrI ACTGG 7 cut(s) 218, 416, 779, 1047, 1750, 2091, 2964
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 2 cut(s) 83, 645
BssT1I CCWWGG 1 cut(s) 83
Bst4CI ACNGT 8 cut(s) 637, 664, 744, 890, 1410, 1478, 1930, 2788
Bst6I CTCTTC 6 cut(s) 26, 494, 844, 865, 1908, 2304
BstAFI CTTAAG 1 cut(s) 2876
BstAPI GCANNNNNTGC 3 cut(s) 758, 1166, 1736
BstAUI TGTACA 2 cut(s) 739, 2398
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 9 cut(s) 763, 1162, 1693, 1706, 1710, 1771, 2222, 2232, 2589
BstDEI CTNAG 8 cut(s) 96, 283, 1395, 1577, 1623, 1869, 2352, 2492
BstDSI CCRYGG 1 cut(s) 83
BstEII GGTNACC 1 cut(s) 992
BstENI CCTNNNNNAGG 2 cut(s) 1187, 2176
BstF5I GGATG 7 cut(s) 60, 956, 1115, 1198, 2000, 2275, 2522
BstFNI CGCG 4 cut(s) 38, 261, 622, 1708
BstHHI GCGC 1 cut(s) 38
BstMAI GTCTC 2 cut(s) 528, 650
BstMCI CGRYCG 1 cut(s) 42
BstNSI RCATGY 2 cut(s) 1364, 1773
BstPAI GACNNNNGTC 1 cut(s) 662
BstPI GGTNACC 1 cut(s) 992
BstSCI CCNGG 1 cut(s) 2603
BstSFI CTRYAG 1 cut(s) 2784
BstUI CGCG 4 cut(s) 38, 261, 622, 1708
BstV2I GAAGAC 1 cut(s) 945
BstX2I RGATCY 2 cut(s) 1954, 2323
BstXI CCANNNNNNTGG 1 cut(s) 2971
BstYI RGATCY 2 cut(s) 1954, 2323
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 2457
BsuRI GGCC 4 cut(s) 41, 88, 1197, 1605
BsuTUI ATCGAT 1 cut(s) 2457
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 7 cut(s) 60, 956, 1115, 1198, 2000, 2275, 2522
BtsIMutI CAGTG 7 cut(s) 786, 1743, 1926, 2098, 2500, 2793, 2877
BtuMI TCGCGA 2 cut(s) 261, 1708
BveI ACCTGC 1 cut(s) 742
Cac8I GCNNGC 9 cut(s) 763, 1162, 1693, 1706, 1710, 1771, 2222, 2232, 2589
CaiI CAGNNNCTG 4 cut(s) 560, 758, 2063, 2327
CciI TCATGA 2 cut(s) 2119, 2565
CfoI GCGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 2 cut(s) 87, 2427
ClaI ATCGAT 1 cut(s) 2457
CseI GACGC 1 cut(s) 939
Csp6I GTAC 4 cut(s) 633, 740, 1095, 2399
CspAI ACCGGT 1 cut(s) 442
CviQI GTAC 4 cut(s) 633, 740, 1095, 2399
DdeI CTNAG 8 cut(s) 96, 283, 1395, 1577, 1623, 1869, 2352, 2492
DraI TTTAAA 1 cut(s) 2530
DriI GACNNNNNGTC 1 cut(s) 1546
EaeI YGGCCR 2 cut(s) 39, 1195
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 6 cut(s) 26, 494, 844, 865, 1908, 2304
Eam1105I GACNNNNNGTC 1 cut(s) 1546
EarI CTCTTC 6 cut(s) 26, 494, 844, 865, 1908, 2304
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 2427
Eco52I CGGCCG 1 cut(s) 39
Eco57I CTGAAG 5 cut(s) 293, 1479, 2328, 2718, 2763
Eco88I CYCGRG 1 cut(s) 1120
Eco91I GGTNACC 1 cut(s) 992
EcoNI CCTNNNNNAGG 2 cut(s) 1187, 2176
EcoO65I GGTNACC 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 1017
EcoT14I CCWWGG 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 83
FalI AAGNNNNNCTT 6 cut(s) 266, 298, 491, 523, 2833, 2865
FaqI GGGAC 1 cut(s) 2520
FbaI TGATCA 4 cut(s) 145, 351, 2497, 2640
FblI GTMKAC 1 cut(s) 2542
FokI GGATG 7 cut(s) 47, 943, 1102, 1205, 2007, 2282, 2509
FspBI CTAG 7 cut(s) 480, 678, 698, 1245, 1674, 2819, 2856
GlaI GCGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 1308
GsuI CTGGAG 3 cut(s) 469, 762, 2787
HaeIII GGCC 4 cut(s) 41, 88, 1197, 1605
HapII CCGG 2 cut(s) 443, 2605
HgaI GACGC 1 cut(s) 939
HhaI GCGC 1 cut(s) 38
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HincII GTYRAC 3 cut(s) 332, 1540, 1794
HindII GTYRAC 3 cut(s) 332, 1540, 1794
HindIII AAGCTT 1 cut(s) 2532
HpaII CCGG 2 cut(s) 443, 2605
HphI GGTGA 6 cut(s) 155, 191, 666, 695, 2787, 2913
Hpy188I TCNGA 6 cut(s) 17, 408, 1398, 1528, 2335, 3006
HpyAV CCTTC 5 cut(s) 1193, 1985, 2091, 2989, 3037
HpyCH4III ACNGT 8 cut(s) 637, 664, 744, 890, 1410, 1478, 1930, 2788
HpyCH4IV ACGT 2 cut(s) 631, 1111
HpyF3I CTNAG 8 cut(s) 96, 283, 1395, 1577, 1623, 1869, 2352, 2492
HpySE526I ACGT 2 cut(s) 631, 1111
HspAI GCGC 1 cut(s) 36
Ksp22I TGATCA 4 cut(s) 145, 351, 2497, 2640
LguI GCTCTTC 2 cut(s) 26, 844
LmnI GCTCC 7 cut(s) 488, 1246, 1632, 1660, 2143, 2248, 2476
LweI GCATC 8 cut(s) 1124, 1453, 2158, 2304, 2458, 2463, 2559, 2642
MaeI CTAG 7 cut(s) 480, 678, 698, 1245, 1674, 2819, 2856
MaeII ACGT 2 cut(s) 631, 1111
MaeIII GTNAC 7 cut(s) 220, 454, 772, 992, 1377, 2129, 2558
MfeI CAATTG 2 cut(s) 1554, 1815
MflI RGATCY 2 cut(s) 1954, 2323
MhlI GDGCHC 1 cut(s) 401
MlsI TGGCCA 1 cut(s) 1197
MluNI TGGCCA 1 cut(s) 1197
MlyI GAGTC 8 cut(s) 212, 257, 566, 1376, 1556, 2027, 2035, 2759
MmeI TCCRAC 4 cut(s) 1460, 2358, 2870, 2895
Mox20I TGGCCA 1 cut(s) 1197
MroXI GAANNNNTTC 1 cut(s) 549
MscI TGGCCA 1 cut(s) 1197
MslI CAYNNNNRTG 3 cut(s) 65, 804, 2211
Msp20I TGGCCA 1 cut(s) 1197
MspA1I CMGCKG 6 cut(s) 347, 1622, 2063, 2372, 2756, 2807
MspCI CTTAAG 1 cut(s) 2876
MspI CCGG 2 cut(s) 443, 2605
MspR9I CCNGG 1 cut(s) 2605
MunI CAATTG 2 cut(s) 1554, 1815
Mva1269I GAATGC 1 cut(s) 791
MvnI CGCG 4 cut(s) 38, 261, 622, 1708
NciI CCSGG 1 cut(s) 2605
NcoI CCATGG 1 cut(s) 83
NlaIV GGNNCC 2 cut(s) 732, 1634
NmeAIII GCCGAG 2 cut(s) 626, 1642
NmuCI GTSAC 2 cut(s) 220, 2558
NruI TCGCGA 2 cut(s) 261, 1708
NspI RCATGY 2 cut(s) 1364, 1773
PaeI GCATGC 1 cut(s) 1773
PaeR7I CTCGAG 1 cut(s) 1120
PagI TCATGA 2 cut(s) 2119, 2565
PciSI GCTCTTC 2 cut(s) 26, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 7 cut(s) 545, 701, 1172, 1213, 1648, 2348, 2918
PflFI GACNNNGTC 1 cut(s) 1846
PflMI CCANNNNNTGG 1 cut(s) 1892
PinAI ACCGGT 1 cut(s) 442
PleI GAGTC 8 cut(s) 211, 257, 565, 1375, 1555, 2026, 2035, 2758
PpsI GAGTC 8 cut(s) 211, 257, 565, 1375, 1555, 2026, 2035, 2758
Ppu21I YACGTR 1 cut(s) 632
PshAI GACNNNNGTC 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 1863
PspEI GGTNACC 1 cut(s) 992
PspFI CCCAGC 1 cut(s) 1304
PspN4I GGNNCC 2 cut(s) 732, 1634
PspPI GGNCC 2 cut(s) 87, 2427
PspXI VCTCGAGB 1 cut(s) 1120
PsrI GAACNNNNNNTAC 2 cut(s) 723, 755
PstNI CAGNNNCTG 4 cut(s) 560, 758, 2063, 2327
PsuI RGATCY 2 cut(s) 1954, 2323
PsyI GACNNNGTC 1 cut(s) 1846
PvuII CAGCTG 5 cut(s) 347, 2063, 2372, 2756, 2807
RruI TCGCGA 2 cut(s) 261, 1708
RsaI GTAC 4 cut(s) 634, 741, 1096, 2400
RsaNI GTAC 4 cut(s) 633, 740, 1095, 2399
RseI CAYNNNNRTG 3 cut(s) 65, 804, 2211
SapI GCTCTTC 2 cut(s) 26, 844
Sau96I GGNCC 2 cut(s) 87, 2427
SchI GAGTC 8 cut(s) 212, 257, 566, 1376, 1556, 2027, 2035, 2759
ScrFI CCNGG 1 cut(s) 2605
SduI GDGCHC 1 cut(s) 401
SfaNI GCATC 8 cut(s) 1124, 1453, 2158, 2304, 2458, 2463, 2559, 2642
SfcI CTRYAG 1 cut(s) 2784
Sfr274I CTCGAG 1 cut(s) 1120
SinI GGWCC 1 cut(s) 2427
SlaI CTCGAG 1 cut(s) 1120
SmiMI CAYNNNNRTG 3 cut(s) 65, 804, 2211
SmlI CTYRAG 4 cut(s) 1120, 1565, 2080, 2876
SmoI CTYRAG 4 cut(s) 1120, 1565, 2080, 2876
SphI GCATGC 1 cut(s) 1773
SsiI CCGC 4 cut(s) 38, 681, 1620, 1773
SspI AATATT 1 cut(s) 1220
SspMI CTAG 7 cut(s) 480, 678, 698, 1245, 1674, 2819, 2856
StyD4I CCNGG 1 cut(s) 2603
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 8 cut(s) 637, 664, 744, 890, 1410, 1478, 1930, 2788
TaiI ACGT 2 cut(s) 634, 1114
TaqI TCGA 6 cut(s) 192, 307, 1121, 1186, 1941, 2457
TatI WGTACW 2 cut(s) 739, 2398
TauI GCSGC 2 cut(s) 41, 1622
TfiI GAWTC 7 cut(s) 545, 701, 1172, 1213, 1648, 2348, 2918
TscAI CASTG 7 cut(s) 786, 1750, 1933, 2098, 2500, 2793, 2877
TseFI GTSAC 2 cut(s) 220, 2558
Tsp45I GTSAC 2 cut(s) 220, 2558
TspGWI ACGGA 3 cut(s) 2528, 2943, 3020
TspRI CASTG 7 cut(s) 786, 1750, 1933, 2098, 2500, 2793, 2877
Tth111I GACNNNGTC 1 cut(s) 1846
Van91I CCANNNNNTGG 1 cut(s) 1892
Vha464I CTTAAG 1 cut(s) 2876
VpaK11BI GGWCC 1 cut(s) 2427
VspI ATTAAT 1 cut(s) 1863
XagI CCTNNNNNAGG 2 cut(s) 1187, 2176
XapI RAATTY 3 cut(s) 1017, 1810, 2377
XceI RCATGY 2 cut(s) 1364, 1773
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 1120
XmiI GTMKAC 1 cut(s) 2542
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 7 cut(s) 480, 678, 698, 1245, 1674, 2819, 2856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.