Rh4BG310600

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003470
Physical Location & Seq
Reverse (-)
47901762 .. 47907730
5969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG310600.1

Sequence Viewer

Length: 3060 bp
ATGGTGCTTGGGATAAGAGCAAAGAGCCGGAAGAGTGCTGCGGTTGAGGTAGATTATGTTATACATGTGCTGGAAATTAAACCATGGCCCTCGTCTCAGGCTTTGAAATCTGTTGAGTCTGTGTTTCTTCAATGGGGAAATGGTGATCAAGCGTCTGGATCATTCTTTCGTAATGTTGGAAGTGAGAAGATTGAATTCGGGGAGTCGTTCAGGCTTCCGGTTGTTTTGTACAAGGAGAAGTCAAGAAAAAGCTCGGCGCGCGACACTTACCAGAAGAACAACTTAGAGTTTCATCTGTCTGCACCTCGAAAGGACAAGGCAGCTAAACTTTTGGGATCGGCTGTGATAAATCTTGCAGATTATGCGACCATAGTTGAAACCACGAATGTCAGAGTTCCACTGAACTTGAAGAAGAGTTCTAAGAGTTCAGCTCAGCCGGTTCTTTATGTTAATGTTCAGCCTTGTGGTAATGATAGCTCCCACTTGGCCAAACAAGTGTCGCTGGACAACCATGGAAGTCATGGGTCTCTGGATGACGAGATTGCCTCTTTTACTGATGATGATGGTGATGATCAGTCTTCACATTCATCACGCACTGTAACCTCTTCTGCTTTTGAGGCACCAGTCAGTTCATCACCAAGTGCTGATAAGAATGCGTCAGAATCAACAATTGATAACACTAGAAGGATTTATGAGGAACCAACCATACATTCAATAGCAGCACCTGCAAACACTGTTATGAATCCAGTCGCTAATGCATTGAAACATCAAAGTGGAAGTTCATCTCCACTGTCGTCAATAGGCTCTTCCTCCCCTCGGAAACCTGCTTATGATTATATTTCTTTGCCTCACCCTTCAAGGGAGAGATCAGTGCCAACCTTGAAAAAATCTGTGACTCAGTCTGTTCAATCATCTTCTTCATCCTCTGGCTATCAAGATGACCATCAAGAGTCTGGTAATTATAATATTAAGACCAACAGAATTCAGAATAGTCTTACTAGCAGAGGTGCCAGAATGCAAGAAAATGCACAAGAATCTACAATGGGCAAAATTGTGAGCAACCATGCTTCTGAAGCTACAACCTCAAGTACGTCTCTGCAGCAGGCTACAAACTCAATATCTGCAAGCTATGCAGATTTAGAATCCCCTCGAGATGATGATCATCTCGTAAAGGTGAATGAATATTCTTTTGATGGCAAATTAGCTTCTAGGTTTTCACAAGATGCTACCAGAAAGCCAGTCACTTTAAAGAGTGAAACTTTTACAGTCAGCAATAATGTTGGATCACCGGAAAGCAAGGTTAAAAGTACTGAATTAAAGCATGTAAAGTCCGTGAAACCTTCTATCTCAGCTGAGACCAATAGTCGGTTAAGAAAACATGAGTTTATGAAAAAGTCAAAGGAAGCTGAGATTCCTGAAGATGGTCGTGTTGGTGGTATCATTAGTGCAAAGTGTGAAAGAGAAGAAACAACAACTAGCTGTTTTGATAGTAAAATTGAGCTGGAGTCTACAATTGAAATGCTTAAGGATGAGTTGAGAGAAGCGGCTGCTGTTGAGGTTGGCCTTTACTCCATTGTCGCCGAGCATGGGAGTAACACGAATAAGATCCATGCTCCTGCTCGCCGACTTTCTAGATTCTATTTTCATGCTTGCAAAATGAGATCTCAAGCTAAGAAGGCAAATGCAGCAAGAGCTGCTATTACTGGATTAATTTTGGTTTCTAAAGCTTGTGGAAATGATGTTCCAAGGTTGACTTTCTGGTTGTCGAACTCAATTGTGTTGAGAGCTATTGTCAGCCAGGGCTTAGGGAAAGCTCAAGTTTCAAATGGACCATGCACTACAATTAAAGGTGGCGAACAGTATGCAGCGGATAGGCATTTATCAGCAAAGGATAGGATTCGTACTCGCAATGATGAGAAAGATAACATTCTTGAAAGTTTTGATAATTGGGAGGATCCACATATATTTATGGTTGCTTTGGAAAAGTTTGAAGCTTGGATATTCTCCCGGATAGTTGAGTCTGTATGGTGGCAGAATATAACTCCACATATGCAGTCTGCAGCTGCGAAAGGCTCAAGCACAAGGAAAGCTAATGGAAGAAAGAAAGGTTTGGGTGATCATGAGCAGGGTAATTTTTCTATTGAGCTGTGGACGAAGGCTTTCAAAGATGCCTGTGAAAGGCTTTGTCCTGTTCGAGCTGGTGGCCATGAGTGTGGCTGCTTGCCTCTTCTAGCTCGTTTGGTCATGGAGCAGTTGGTGAGTAGGTTGGATGTGGCTATGTTCAATGCTATTCTTCGTGAAAATGCTGAAGAGATGCCCACAGATCCCGTGTCCGACCCTATAAGTGATTCCAAGGTTCTCCCTATTCCAGCTGGAAAATCCAGTTTTGGGGCAGGTGCACAGCTAAAAAATGCGATTGGAAGCTGGTCCAGATGGCTGACTGATCTATTTGGTATGGATGATACTGATGTTCCAGATGATGAGGATGAACTCAGTGATCACAAGGGGCAGGAGAGTGAAACATCCTTTAAAGCTTTCCGTCTTCTTAATGCTTTGAGTGATCTCATGATGCTTCCATCTGAAATGCTTGCAGATAAATCCACCAGAGAAGAGGTATGCCCTACATTCGGTGCATCAATGATCAAGAGGGTCCTTTACAATTTTGTTACAGATGAGTTTTGCCCAGACCCGATTCCCGAAGCAGTTTTTGAGGCTCTTGACAATGAGGAGAATCTGGAAGCTGAAACAGAATCTGTTACAAGTTTCCCATTTATTGCAAATCCCACATTTTATTCACCACCTCCAGCTGCTGCTTCTCTATCAGGCATTACAGGAGAGGTGGGGAGCCCAGCTCACCTAAGGAGTGGGTCATCAGTGCTCAAGAAATCATATACTAGTGATGATGAGCTTGACGAGTTGGATTCACCGATGACTTCAATCGTAGAAAATTCCCAGGTTTCCCCCAAGTCGTTGACAGCCAACCCAATGCTGAAGTGGAAGGGTGGTCGGAAAGTTGTCCGATATCAACTCCTCCGTCAGGTTTGGAAGGACAGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1019

Amino Acids

111.72

Weight (kDa)

6.12

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 12 - 159 2.7e-15 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 963
AarI CACCTGC 2 cut(s) 733, 2384
Acc36I ACCTGC 3 cut(s) 733, 832, 2384
AccB1I GGYRCC 2 cut(s) 619, 1007
AccI GTMKAC 1 cut(s) 1508
AccII CGCG 2 cut(s) 259, 261
AciI CCGC 3 cut(s) 41, 1544, 1868
AclWI GGATC 7 cut(s) 166, 343, 1291, 1600, 1949, 1962, 2318
AcoI YGGCCR 2 cut(s) 486, 2203
AcsI RAATTY 3 cut(s) 194, 981, 2947
AcuI CTGAAG 4 cut(s) 1092, 1437, 2328, 3011
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 4 cut(s) 230, 1090, 1309, 1903
AfiI CCNNNNNNNGG 9 cut(s) 816, 859, 1365, 1421, 1587, 2178, 2388, 2627, 3037
AflII CTTAAG 1 cut(s) 1523
AflIII ACRYGT 1 cut(s) 64
AhdI GACNNNNNGTC 1 cut(s) 1362
AhlI ACTAGT 1 cut(s) 2894
AjnI CCWGG 2 cut(s) 1797, 2952
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AloI GAACNNNNNNTCC 2 cut(s) 1725, 1757
Alw21I GWGCWC 2 cut(s) 2401, 2880
Alw26I GTCTC 4 cut(s) 99, 531, 1098, 1349
Alw44I GTGCAC 1 cut(s) 2397
AlwI GGATC 7 cut(s) 166, 343, 1291, 1600, 1949, 1962, 2318
AlwNI CAGNNNCTG 4 cut(s) 725, 2753, 2810, 3057
Ama87I CYCGRG 1 cut(s) 1149
AoxI GGCC 4 cut(s) 86, 486, 1561, 2203
ApaLI GTGCAC 1 cut(s) 2397
ApoI RAATTY 3 cut(s) 194, 981, 2947
AseI ATTAAT 1 cut(s) 1709
Asp700I GAANNNNTTC 1 cut(s) 2159
AspLEI GCGC 2 cut(s) 259, 261
AspS9I GGNCC 4 cut(s) 87, 1829, 2427, 2650
AsuC2I CCSGG 1 cut(s) 2008
AvaI CYCGRG 1 cut(s) 1149
AvaII GGWCC 3 cut(s) 1829, 2427, 2650
AxyI CCTNAGG 1 cut(s) 2858
BaeGI GKGCMC 1 cut(s) 2401
BaeI ACNNNNGTAYC 2 cut(s) 2454, 2487
BalI TGGCCA 2 cut(s) 488, 2205
BamHI GGATCC 1 cut(s) 1954
BanI GGYRCC 2 cut(s) 619, 1007
BanII GRGCYC 1 cut(s) 2849
BbsI GAAGAC 2 cut(s) 570, 2534
Bbv12I GWGCWC 2 cut(s) 2401, 2880
BccI CCATC 6 cut(s) 557, 951, 1187, 1415, 2427, 2584
BcgI CGANNNNNNTGC 2 cut(s) 1844, 1878
BciT130I CCWGG 2 cut(s) 1799, 2954
BclI TGATCA 6 cut(s) 145, 571, 1159, 2116, 2497, 2640
BcnI CCSGG 1 cut(s) 2008
BcoDI GTCTC 4 cut(s) 99, 531, 1098, 1349
BcuI ACTAGT 1 cut(s) 2894
BfaI CTAG 7 cut(s) 681, 999, 1209, 1476, 1632, 2231, 2895
BfmI CTRYAG 2 cut(s) 1097, 2058
BfrI CTTAAG 1 cut(s) 1523
BfuAI ACCTGC 3 cut(s) 733, 832, 2384
BglII AGATCT 1 cut(s) 1661
BlpI GCTNAGC 1 cut(s) 432
BmcAI AGTACT 1 cut(s) 1309
Bme1390I CCNGG 3 cut(s) 1799, 2008, 2954
Bme18I GGWCC 3 cut(s) 1829, 2427, 2650
BmeRI GACNNNNNGTC 1 cut(s) 1362
BmeT110I CYCGRG 1 cut(s) 1149
BmgT120I GGNCC 4 cut(s) 87, 1829, 2427, 2650
BmiI GGNNCC 6 cut(s) 621, 699, 1009, 1956, 2651, 2846
BmrFI CCNGG 3 cut(s) 1799, 2008, 2954
BmsI GCATC 5 cut(s) 1213, 2158, 2304, 2559, 2642
BpiI GAAGAC 2 cut(s) 570, 2534
BplI GAGNNNNNCTC 6 cut(s) 415, 447, 530, 562, 2836, 2868
BpmI CTGGAG 2 cut(s) 1523, 2787
Bpu10I CCTNAGC 1 cut(s) 1804
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 5 cut(s) 1069, 1650, 1800, 2059, 2864
BpuMI CCSGG 1 cut(s) 2008
BsaBI GATNNNNATC 3 cut(s) 942, 1158, 1161
BsaI GGTCTC 2 cut(s) 531, 1349
BsaJI CCNNGG 7 cut(s) 83, 511, 815, 1745, 1798, 2352, 2952
BsaWI WCCGGW 2 cut(s) 217, 1288
Bsc4I CCNNNNNNNGG 9 cut(s) 816, 859, 1365, 1421, 1587, 2178, 2388, 2627, 3037
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 5 cut(s) 623, 746, 1238, 1708, 2382
Bse21I CCTNAGG 1 cut(s) 2858
Bse3DI GCAATG 1 cut(s) 1915
Bse8I GATNNNNATC 3 cut(s) 942, 1158, 1161
BseBI CCWGG 2 cut(s) 1799, 2954
BseDI CCNNGG 7 cut(s) 83, 511, 815, 1745, 1798, 2352, 2952
BseGI GGATG 7 cut(s) 538, 920, 1534, 2275, 2464, 2491, 2522
BseJI GATNNNNATC 3 cut(s) 942, 1158, 1161
BseLI CCNNNNNNNGG 9 cut(s) 816, 859, 1365, 1421, 1587, 2178, 2388, 2627, 3037
BseMI GCAATG 1 cut(s) 1915
BseMII CTCAG 7 cut(s) 110, 446, 911, 1344, 1362, 1398, 2506
BseNI ACTGG 5 cut(s) 623, 746, 1238, 1708, 2382
BsePI GCGCGC 1 cut(s) 257
BseRI GAGGAG 2 cut(s) 2741, 3020
BseSI GKGCMC 1 cut(s) 2401
BseYI CCCAGC 1 cut(s) 2848
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 259, 261
BshFI GGCC 4 cut(s) 88, 488, 1563, 2205
BshNI GGYRCC 2 cut(s) 619, 1007
BsiHKAI GWGCWC 2 cut(s) 2401, 2880
BsiHKCI CYCGRG 1 cut(s) 1149
BsiSI CCGG 5 cut(s) 28, 218, 437, 1289, 2008
BslI CCNNNNNNNGG 9 cut(s) 816, 859, 1365, 1421, 1587, 2178, 2388, 2627, 3037
BsmAI GTCTC 4 cut(s) 99, 531, 1098, 1349
BsmBI CGTCTC 2 cut(s) 99, 1098
BsmI GAATGC 2 cut(s) 658, 1020
BsnI GGCC 4 cut(s) 88, 488, 1563, 2205
Bso31I GGTCTC 2 cut(s) 531, 1349
BsoBI CYCGRG 1 cut(s) 1149
Bsp1286I GDGCHC 3 cut(s) 2401, 2849, 2880
Bsp1407I TGTACA 1 cut(s) 228
Bsp1720I GCTNAGC 1 cut(s) 432
Bsp19I CCATGG 2 cut(s) 83, 511
BspACI CCGC 3 cut(s) 41, 1544, 1868
BspANI GGCC 4 cut(s) 88, 488, 1563, 2205
BspCNI CTCAG 7 cut(s) 109, 445, 910, 1345, 1361, 1399, 2505
BspFNI CGCG 2 cut(s) 259, 261
BspHI TCATGA 2 cut(s) 2119, 2565
BspLI GGNNCC 6 cut(s) 621, 699, 1009, 1956, 2651, 2846
BspMAI CTGCAG 2 cut(s) 1101, 2062
BspMI ACCTGC 3 cut(s) 733, 832, 2384
BspPI GGATC 7 cut(s) 166, 343, 1291, 1600, 1949, 1962, 2318
BspQI GCTCTTC 1 cut(s) 811
BspT107I GGYRCC 2 cut(s) 619, 1007
BspTI CTTAAG 1 cut(s) 1523
BspTNI GGTCTC 2 cut(s) 531, 1349
BsrDI GCAATG 1 cut(s) 1915
BsrFI RCCGGY 1 cut(s) 436
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 5 cut(s) 623, 746, 1238, 1708, 2382
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 7 cut(s) 83, 511, 815, 1745, 1798, 2352, 2952
BssHII GCGCGC 1 cut(s) 257
BssT1I CCWWGG 4 cut(s) 83, 511, 1745, 2352
Bst2UI CCWGG 2 cut(s) 1799, 2954
Bst4CI ACNGT 6 cut(s) 598, 736, 792, 1267, 1860, 3053
Bst6I CTCTTC 7 cut(s) 26, 407, 610, 811, 2232, 2304, 2604
BstAFI CTTAAG 1 cut(s) 1523
BstAPI GCANNNNNTGC 4 cut(s) 362, 725, 1130, 1694
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 7 cut(s) 259, 1104, 1126, 1621, 1651, 2222, 2589
BstDSI CCRYGG 2 cut(s) 83, 511
BstENI CCTNNNNNAGG 2 cut(s) 2176, 3035
BstF5I GGATG 7 cut(s) 538, 920, 1534, 2275, 2464, 2491, 2522
BstFNI CGCG 2 cut(s) 259, 261
BstHHI GCGC 2 cut(s) 259, 261
BstMAI GTCTC 4 cut(s) 99, 531, 1098, 1349
BstNI CCWGG 2 cut(s) 1799, 2954
BstNSI RCATGY 2 cut(s) 68, 1325
BstSCI CCNGG 3 cut(s) 1797, 2006, 2952
BstSFI CTRYAG 2 cut(s) 1097, 2058
BstSLI GKGCMC 1 cut(s) 2401
BstUI CGCG 2 cut(s) 259, 261
BstV2I GAAGAC 2 cut(s) 570, 2534
BstX2I RGATCY 4 cut(s) 1605, 1661, 1954, 2323
BstXI CCANNNNNNTGG 1 cut(s) 2213
BstYI RGATCY 4 cut(s) 1605, 1661, 1954, 2323
Bsu36I CCTNAGG 1 cut(s) 2858
BsuRI GGCC 4 cut(s) 88, 488, 1563, 2205
BtgI CCRYGG 2 cut(s) 83, 511
BtsCI GGATG 7 cut(s) 538, 920, 1534, 2275, 2464, 2491, 2522
BtsIMutI CAGTG 8 cut(s) 398, 594, 732, 788, 876, 2500, 2880, 3058
BveI ACCTGC 3 cut(s) 733, 832, 2384
Cac8I GCNNGC 7 cut(s) 259, 1104, 1126, 1621, 1651, 2222, 2589
CaiI CAGNNNCTG 4 cut(s) 725, 2753, 2810, 3057
CciI TCATGA 2 cut(s) 2119, 2565
CfoI GCGC 2 cut(s) 259, 261
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 4 cut(s) 87, 1829, 2427, 2650
CseI GACGC 2 cut(s) 141, 645
Csp6I GTAC 4 cut(s) 229, 1089, 1308, 1902
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviQI GTAC 4 cut(s) 229, 1089, 1308, 1902
DraI TTTAAA 2 cut(s) 1248, 2530
DraIII CACNNNGTG 1 cut(s) 641
DriI GACNNNNNGTC 1 cut(s) 1362
EaeI YGGCCR 2 cut(s) 486, 2203
Eam1104I CTCTTC 7 cut(s) 26, 407, 610, 811, 2232, 2304, 2604
Eam1105I GACNNNNNGTC 1 cut(s) 1362
EarI CTCTTC 7 cut(s) 26, 407, 610, 811, 2232, 2304, 2604
Eco130I CCWWGG 4 cut(s) 83, 511, 1745, 2352
Eco24I GRGCYC 1 cut(s) 2849
Eco31I GGTCTC 2 cut(s) 531, 1349
Eco32I GATATC 1 cut(s) 3023
Eco47I GGWCC 3 cut(s) 1829, 2427, 2650
Eco57I CTGAAG 4 cut(s) 1092, 1437, 2328, 3011
Eco81I CCTNAGG 1 cut(s) 2858
Eco88I CYCGRG 1 cut(s) 1149
EcoNI CCTNNNNNAGG 2 cut(s) 2176, 3035
EcoO109I RGGNCCY 1 cut(s) 2650
EcoRI GAATTC 2 cut(s) 194, 981
EcoRII CCWGG 2 cut(s) 1797, 2952
EcoRV GATATC 1 cut(s) 3023
EcoT14I CCWWGG 4 cut(s) 83, 511, 1745, 2352
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 1 cut(s) 2849
ErhI CCWWGG 4 cut(s) 83, 511, 1745, 2352
Esp3I CGTCTC 2 cut(s) 99, 1098
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 1738, 1770
FauNDI CATATG 1 cut(s) 2049
FbaI TGATCA 6 cut(s) 145, 571, 1159, 2116, 2497, 2640
FblI GTMKAC 1 cut(s) 1508
FokI GGATG 7 cut(s) 545, 907, 1541, 2282, 2471, 2498, 2509
FriOI GRGCYC 1 cut(s) 2849
FspBI CTAG 7 cut(s) 681, 999, 1209, 1476, 1632, 2231, 2895
GlaI GCGC 2 cut(s) 258, 260
GsaI CCCAGC 1 cut(s) 2852
GsuI CTGGAG 2 cut(s) 1523, 2787
HaeIII GGCC 4 cut(s) 88, 488, 1563, 2205
HapII CCGG 5 cut(s) 28, 218, 437, 1289, 2008
HgaI GACGC 2 cut(s) 141, 645
HhaI GCGC 2 cut(s) 259, 261
Hin6I GCGC 2 cut(s) 257, 259
HinP1I GCGC 2 cut(s) 257, 259
HincII GTYRAC 2 cut(s) 1752, 2973
HindII GTYRAC 2 cut(s) 1752, 2973
HindIII AAGCTT 3 cut(s) 1725, 1992, 2532
HpaII CCGG 5 cut(s) 28, 218, 437, 1289, 2008
Hpy166II GTNNAC 5 cut(s) 1509, 1752, 2151, 2399, 2973
Hpy188I TCNGA 9 cut(s) 392, 661, 819, 987, 1072, 2335, 2581, 3009, 3020
Hpy8I GTNNAC 5 cut(s) 1509, 1752, 2151, 2399, 2973
HpyAV CCTTC 7 cut(s) 678, 864, 1350, 1669, 2149, 2992, 3040
HpyCH4III ACNGT 6 cut(s) 598, 736, 792, 1267, 1860, 3053
HpyCH4IV ACGT 1 cut(s) 1091
HpySE526I ACGT 1 cut(s) 1091
HspAI GCGC 2 cut(s) 257, 259
Ksp22I TGATCA 6 cut(s) 145, 571, 1159, 2116, 2497, 2640
LguI GCTCTTC 1 cut(s) 811
LmnI GCTCC 4 cut(s) 482, 1618, 2248, 2844
LweI GCATC 5 cut(s) 1213, 2158, 2304, 2559, 2642
MaeI CTAG 7 cut(s) 681, 999, 1209, 1476, 1632, 2231, 2895
MaeII ACGT 1 cut(s) 1091
MaeIII GTNAC 7 cut(s) 598, 892, 1240, 1592, 2665, 2755, 3053
MfeI CAATTG 3 cut(s) 669, 1512, 1773
MflI RGATCY 4 cut(s) 1605, 1661, 1954, 2323
MhlI GDGCHC 3 cut(s) 2401, 2849, 2880
MlsI TGGCCA 2 cut(s) 488, 2205
MluNI TGGCCA 2 cut(s) 488, 2205
MlyI GAGTC 6 cut(s) 125, 212, 889, 959, 1514, 2027
MmeI TCCRAC 6 cut(s) 157, 1261, 2247, 2358, 2898, 2987
Mox20I TGGCCA 2 cut(s) 488, 2205
Mph1103I ATGCAT 1 cut(s) 760
MroXI GAANNNNTTC 1 cut(s) 2159
MscI TGGCCA 2 cut(s) 488, 2205
MslI CAYNNNNRTG 3 cut(s) 737, 771, 2211
Msp20I TGGCCA 2 cut(s) 488, 2205
MspA1I CMGCKG 5 cut(s) 1352, 1868, 2063, 2372, 2807
MspCI CTTAAG 1 cut(s) 1523
MspI CCGG 5 cut(s) 28, 218, 437, 1289, 2008
MspR9I CCNGG 3 cut(s) 1799, 2008, 2954
MunI CAATTG 3 cut(s) 669, 1512, 1773
Mva1269I GAATGC 2 cut(s) 658, 1020
MvaI CCWGG 2 cut(s) 1799, 2954
MvnI CGCG 2 cut(s) 259, 261
NciI CCSGG 1 cut(s) 2008
NcoI CCATGG 2 cut(s) 83, 511
NdeI CATATG 1 cut(s) 2049
NlaIV GGNNCC 6 cut(s) 621, 699, 1009, 1956, 2651, 2846
NmeAIII GCCGAG 2 cut(s) 233, 1606
NmuCI GTSAC 3 cut(s) 892, 1240, 3053
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 2 cut(s) 68, 1325
PaeR7I CTCGAG 1 cut(s) 1149
PagI TCATGA 2 cut(s) 2119, 2565
PaqCI CACCTGC 2 cut(s) 733, 2384
PauI GCGCGC 1 cut(s) 257
PciI ACATGT 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 811
PctI GAATGC 2 cut(s) 658, 1020
PdmI GAANNNNTTC 1 cut(s) 2159
PflFI GACNNNGTC 1 cut(s) 898
PfoI TCCNGGA 1 cut(s) 2006
PleI GAGTC 6 cut(s) 124, 211, 889, 958, 1513, 2026
PpsI GAGTC 6 cut(s) 124, 211, 889, 958, 1513, 2026
PpuMI RGGWCCY 1 cut(s) 2650
PscI ACATGT 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 1709
PsiI TTATAA 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 2650
Psp6I CCWGG 2 cut(s) 1797, 2952
PspFI CCCAGC 1 cut(s) 2848
PspGI CCWGG 2 cut(s) 1797, 2952
PspN4I GGNNCC 6 cut(s) 621, 699, 1009, 1956, 2651, 2846
PspPI GGNCC 4 cut(s) 87, 1829, 2427, 2650
PspPPI RGGWCCY 1 cut(s) 2650
PsrI GAACNNNNNNTAC 2 cut(s) 690, 722
PstI CTGCAG 2 cut(s) 1101, 2062
PstNI CAGNNNCTG 4 cut(s) 725, 2753, 2810, 3057
PsuI RGATCY 4 cut(s) 1605, 1661, 1954, 2323
PsyI GACNNNGTC 1 cut(s) 898
PteI GCGCGC 1 cut(s) 257
PvuII CAGCTG 4 cut(s) 1352, 2063, 2372, 2807
RsaI GTAC 4 cut(s) 230, 1090, 1309, 1903
RsaNI GTAC 4 cut(s) 229, 1089, 1308, 1902
RseI CAYNNNNRTG 3 cut(s) 737, 771, 2211
SapI GCTCTTC 1 cut(s) 811
Sau96I GGNCC 4 cut(s) 87, 1829, 2427, 2650
ScaI AGTACT 1 cut(s) 1309
SchI GAGTC 6 cut(s) 125, 212, 889, 959, 1514, 2027
ScrFI CCNGG 3 cut(s) 1799, 2008, 2954
SduI GDGCHC 3 cut(s) 2401, 2849, 2880
SfaNI GCATC 5 cut(s) 1213, 2158, 2304, 2559, 2642
SfcI CTRYAG 2 cut(s) 1097, 2058
Sfr274I CTCGAG 1 cut(s) 1149
SinI GGWCC 3 cut(s) 1829, 2427, 2650
SlaI CTCGAG 1 cut(s) 1149
SmiMI CAYNNNNRTG 3 cut(s) 737, 771, 2211
SmlI CTYRAG 7 cut(s) 1084, 1149, 1523, 1665, 1815, 2074, 2879
SmoI CTYRAG 7 cut(s) 1084, 1149, 1523, 1665, 1815, 2074, 2879
SpeI ACTAGT 1 cut(s) 2894
SsiI CCGC 3 cut(s) 41, 1544, 1868
SspI AATATT 2 cut(s) 967, 1184
SspMI CTAG 7 cut(s) 681, 999, 1209, 1476, 1632, 2231, 2895
StyD4I CCNGG 3 cut(s) 1797, 2006, 2952
StyI CCWWGG 4 cut(s) 83, 511, 1745, 2352
TaaI ACNGT 6 cut(s) 598, 736, 792, 1267, 1860, 3053
TaiI ACGT 1 cut(s) 1094
TaqI TCGA 4 cut(s) 307, 1150, 1766, 2194
TatI WGTACW 2 cut(s) 228, 1307
TauI GCSGC 1 cut(s) 1547
TscAI CASTG 8 cut(s) 405, 601, 739, 795, 876, 2500, 2880, 3058
TseFI GTSAC 3 cut(s) 892, 1240, 3053
Tsp45I GTSAC 3 cut(s) 892, 1240, 3053
TspGWI ACGGA 3 cut(s) 1321, 2528, 3023
TspRI CASTG 8 cut(s) 405, 601, 739, 795, 876, 2500, 2880, 3058
Tth111I GACNNNGTC 1 cut(s) 898
Vha464I CTTAAG 1 cut(s) 1523
VneI GTGCAC 1 cut(s) 2397
VpaK11BI GGWCC 3 cut(s) 1829, 2427, 2650
VspI ATTAAT 1 cut(s) 1709
XagI CCTNNNNNAGG 2 cut(s) 2176, 3035
XapI RAATTY 3 cut(s) 194, 981, 2947
XbaI TCTAGA 1 cut(s) 1631
XceI RCATGY 2 cut(s) 68, 1325
XcmI CCANNNNNNNNNTGG 3 cut(s) 518, 950, 2991
XhoI CTCGAG 1 cut(s) 1149
XmiI GTMKAC 1 cut(s) 1508
XmnI GAANNNNTTC 1 cut(s) 2159
XspI CTAG 7 cut(s) 681, 999, 1209, 1476, 1632, 2231, 2895
ZrmI AGTACT 1 cut(s) 1309
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.