MD16G1115400.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003470
Physical Location & Seq
Forward (+)
8224297 .. 8231226
6930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1115400.v1.1.491

Sequence Viewer

Length: 3072 bp
ATGGTTCTTGGGATAAGATCAAAGAGCAGGAAGAGCGCGGCCGTTGAGGTTGATTACCTCATCCATGTGCAGGAACTAAAGCCATGGCCCTCGTCTCAGGCTTTGAGATCCGTTCAGTCCGTTATGCTTCAGTGGGAAAACGGTGATCAGGTTTCTGGATCGTGTATTTGCAATGTTGGTGATGGAAAGATCGAATTTGGCGAGTCTTTCACGCTTCCAGTCACTTTATACAAGGAGAAATCAAGAAAAAACGCAGTTCGCGACACTTACCAGAAGAACAACTTGGAGTTTTACTTGTATGAACCTCGAAAGGACAAGGGAGTGAAAGGTCAACTCTTGGGGTCGGCTGAGATCAATCTTGCAGACTATGGGATCGTTGTGGAAACCAGAAATGTGAGCACTCCGCTGAACTGGAAGAAGAGTTTTAAGAGTTCAGCACAAACTGTTCTTTCTGTTAGTATTCAGCCGTGTGGTAAACCGAGCTCCAGCTTGACACCAAAGAGCAGCTTGTCCAGAGAAGAGTCTCTGGAAAATGAGGGAACTGAATCTGTTCCAGGGTCTGTGAATGATGGCACTGACGAGATTGCCTCTTTTACCGATGATGATGTTGATGATGATGTTTCCTCGCATCCATCACACACTGTAAACTCCTCGGCTGTTGAGACAACAGTCAGTTCATCACCTAGCGGTGAAAAGAATGTATTAGAATCAACAGCCAATGGCACTAGGAGGACTTATGGGGAGCCTGCTGCACGGTCAATAGCAGCACCTGGAAGCACAGGGGTGACTCCAGTGGTTAAAGCATTAGAGCACCAAAATGGAAGTTCATCTCCACTGTCGTCAATAGGCTCTTCCTCCATTCTGCTGAATTCTGCAAATGATCGTGCTTCTTTGCCTAACAGTTCAAAGGAGAGTGCTTCAATGCCAATCCTGAAAAAATCAATCACCCACTCTGTTCATTCAGCGTCTTCACCCTTTGGCTATCAAGACAGCCATCAGGAATCTGGTTACCATAATTTTAAGGACAACAGAATTCACATAACTCGTTCTAACAGAAGTGCCAGAGTGCATGAAAATGCTCAAGATCTTATCAAAGACAATATTGTCCGTAACCATGCTGCTGAAGGTTCATCCTCAAGCATGTCTATTCAGGAGGACACAAACTCCACATTTGCAAGCAATGCAGATTCACAAGCCTTTCGAGAGGATGGTCATCTCTTAAAGGCCAATCAGTATACTTTTGATGACAAATTAGCTTCTCGATTTTCACAAGATGCTACCAGAAAACAAGTCAGGTTAAAGAGTGAAACTTTTACACCCAGCAGGAGAGTTGTTGGAGTGCAGGGAAGTAAGGTTAAAAGTAATGAACTAAAACATGTAAAGTCTGTGCAGTTACCTTTAGTATCAGCTCAGAACAATAGACAGTCAAGCAATAATGAGCTTCTCGAAAAGTCAAAGGAAGCTGAAACTCAAGATGCTTATGTTCATGGTCGAATTAGCGCGACAAGTGAAAGAGAACAAAAAACAGTCAGCTTTTCCGATGGCAAAGTTGACTTGGAATCTACAATTGAATTGCTTAAGGAAGAACTGAGAGAAGCTGCTGCTGTGGAGATTGGCCTTTACTCTGTTGCTGCTGAGCATGGGAGCCACACAAATAAGATTCATGCTCCTGCTCGGCGACTTTCTAGATTCTATTTTCATGCTTGCAAAACAAGCTTTGGAGCTAAGAAGGCAAATGCAGCACGAGCTGCTATCACTGGACTAATCTTGGTTTCTAAAGCATGCGGAAATGATGTTGCAAGGTTGACATTCTGGTTGTCGAATTCAATTGTGTTGAGAGCTATTATCAGCCAGTCCTTGCCCTGCAGAAAAGTTGGCGCTGAGGGGGTTTTAAAAGCAAAGAATGGGTTTCTTCCTCACAAGGAAGAGAACAATTGCACTCTTGAAAGTTTTGATGATTGGGAGGATCCGCAGATATTTATGGCTGCTTTGGAAAAGTTTGAAGGTTGGATCTTCTCCCGAATTGTTGAGTCTGTATGGTGGCAGAATATGACTCCATATATGCAGTCAGCGGTTGCGAAGGGCTCAAGCTCAAGGAAAGCCAATGGAAGGAAGCATGGTTTGGGTGGTCATGAGCAAGGTAACTTTTCTATGGAGCTGTGGAAGAAGGCTTTCAAAGATGCCTGTGAAAGGCTCTGTCCTGCAAGAGCTGTTGGACATGAGTGTGGCTGCTTGCCTCTGCTGGCTCGTTTGGTAATGGAGCAATTAGTGGATAGATTGGATGTGGCTATGTTCAATGCTATTCTTCGTGAAAATGCTGAAGAGATGCCCACAGATCCTGTGTCCGACCCCATAAGTGATTCTAAGGTTCTCCCTATTCCAGCTGGAAAGTCGAGCTTTGGGGCTGGTGCACAGCTAAAAAATGCGATTGGAAGCTGGTCTAGATGGCTTACTGATCTATTTGGCATCGATGATACTGATGCCCCTGATGATAATACTGAACTCAGTGATCACAAGGGACAAGAGTGCCCAACATCCTTTAAAGCTTTCCGTCTTCTTAATGCTTTGAGTGACCTTATGATGCTTCCATTTGATATGCTGGCAGATAAATCCACCCGGAAAGAGGTATGCCCTACATTTGGTGCATCATTGATCAAGAGGGTTATATACAATTTTGTTCCAGATGAGTTTTGCCCAGACCCAATTCCCACAGCAGTTTTTGAGGCTCTTGATTATGAGGAGAATTCAGAAGCTAAAACCGACTCTGCTGGAAGTTTCCCATGCAACGCAAATCCTACAATCTATTCACCACCTCCAGCTGCTTCGCTATTAAGCATTATAGGAGAGGTAGGAAGTCTAACTCTCTCGAGGAGTGGGTCATCAGTGCTTAAGAAATCGTATACAAGTGATGATGAGCTCGATGAGTTGGATTCACCGATGACATCAATCATCATAGACAATTCCCCCTTTTCTCCCAGTCCATTGGCAGCCAACTCAACACCGAAGTGGAAGGGTGGTCGGAATTCTGTCAGGTATCAACTCCTCCGTGAAGTTTGGAAGGATAGCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1024

Amino Acids

111.8

Weight (kDa)

6.04

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 12 - 156 1.9e-15 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1103
AccI GTMKAC 2 cut(s) 1235, 2900
AccII CGCG 3 cut(s) 38, 261, 1502
AciI CCGC 6 cut(s) 38, 404, 687, 1785, 1970, 2072
AclWI GGATC 7 cut(s) 102, 166, 380, 1961, 1974, 2018, 2330
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 6 cut(s) 194, 868, 1032, 1822, 2743, 3022
AcuI CTGAAG 3 cut(s) 113, 1143, 2340
AfiI CCNNNNNNNGG 5 cut(s) 70, 2109, 2190, 2623, 2639
AflII CTTAAG 2 cut(s) 1577, 2888
AflIII ACRYGT 1 cut(s) 1375
AgsI TTSAA 8 cut(s) 906, 921, 1571, 1827, 1946, 2003, 2176, 2296
AjnI CCWGG 2 cut(s) 553, 769
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 2105, 2137
AleI CACNNNNGTG 2 cut(s) 782, 3004
Alw21I GWGCWC 5 cut(s) 401, 485, 813, 2413, 2919
Alw26I GTCTC 3 cut(s) 99, 528, 656
Alw44I GTGCAC 1 cut(s) 2409
AlwI GGATC 7 cut(s) 102, 166, 380, 1961, 1974, 2018, 2330
AlwNI CAGNNNCTG 3 cut(s) 560, 770, 2339
Ama87I CYCGRG 1 cut(s) 2866
AoxI GGCC 4 cut(s) 39, 86, 1224, 1615
ApaLI GTGCAC 1 cut(s) 2409
ApoI RAATTY 6 cut(s) 194, 868, 1032, 1822, 2743, 3022
Asp700I GAANNNNTTC 2 cut(s) 549, 2171
AspLEI GCGC 3 cut(s) 38, 1502, 1880
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 1 cut(s) 2617
AsuHPI GGTGA 9 cut(s) 155, 191, 672, 701, 796, 937, 963, 2799, 2925
AvaI CYCGRG 1 cut(s) 2866
BaeGI GKGCMC 2 cut(s) 2413, 2531
BamHI GGATCC 1 cut(s) 1966
BanII GRGCYC 3 cut(s) 485, 2087, 2919
BauI CACGAG 1 cut(s) 1743
BbsI GAAGAC 2 cut(s) 960, 2546
Bbv12I GWGCWC 5 cut(s) 401, 485, 813, 2413, 2919
BbvCI CCTCAGC 1 cut(s) 1881
BccI CCATC 7 cut(s) 176, 563, 640, 1002, 1202, 1535, 2439
BceAI ACGGC 2 cut(s) 26, 451
BciT130I CCWGG 2 cut(s) 555, 771
BclI TGATCA 3 cut(s) 145, 2509, 2652
BcnI CCSGG 1 cut(s) 2617
BcoDI GTCTC 3 cut(s) 99, 528, 656
BfaI CTAG 4 cut(s) 684, 726, 1686, 2442
BfmI CTRYAG 1 cut(s) 1864
BfoI RGCGCY 1 cut(s) 1881
BfrI CTTAAG 2 cut(s) 1577, 2888
BglII AGATCT 1 cut(s) 1084
BlpI GCTNAGC 1 cut(s) 1635
Bme1390I CCNGG 3 cut(s) 555, 771, 2617
BmeT110I CYCGRG 1 cut(s) 2866
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 744, 1646, 1968
BmrFI CCNGG 3 cut(s) 555, 771, 2617
BmrI ACTGGG 1 cut(s) 2970
BmsI GCATC 9 cut(s) 637, 1264, 1465, 2170, 2316, 2470, 2475, 2571, 2654
BmuI ACTGGG 1 cut(s) 2970
BoxI GACNNNNGTC 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 960, 2546
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 3 cut(s) 469, 774, 2799
Bpu10I CCTNAGC 1 cut(s) 1881
Bpu1102I GCTNAGC 1 cut(s) 1635
BpuEI CTTGAG 5 cut(s) 1065, 1120, 1455, 2071, 2077
BpuMI CCSGG 1 cut(s) 2617
Bsa29I ATCGAT 1 cut(s) 2469
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 3 cut(s) 83, 554, 651
BsaXI ACNNNNNCTCC 2 cut(s) 1148, 1178
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 2109, 2190, 2623, 2639
Bse1I ACTGG 6 cut(s) 218, 416, 791, 1762, 1852, 2976
Bse3DI GCAATG 2 cut(s) 178, 1186
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 555, 771
BseCI ATCGAT 1 cut(s) 2469
BseDI CCNNGG 3 cut(s) 83, 554, 651
BseGI GGATG 6 cut(s) 60, 628, 1130, 1213, 2287, 2534
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 5 cut(s) 70, 2109, 2190, 2623, 2639
BseMI GCAATG 2 cut(s) 178, 1186
BseMII CTCAG 7 cut(s) 110, 339, 1424, 1580, 1626, 1872, 2518
BseNI ACTGG 6 cut(s) 218, 416, 791, 1762, 1852, 2976
BseRI GAGGAG 4 cut(s) 640, 2753, 2884, 3032
BseSI GKGCMC 2 cut(s) 2413, 2531
BseX3I CGGCCG 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 1319
BsgI GTGCAG 4 cut(s) 89, 735, 1361, 1409
Bsh1236I CGCG 3 cut(s) 38, 261, 1502
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 41, 88, 1226, 1617
BshVI ATCGAT 1 cut(s) 2469
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 5 cut(s) 401, 485, 813, 2413, 2919
BsiHKCI CYCGRG 1 cut(s) 2866
BsiSI CCGG 1 cut(s) 2617
BslFI GGGAC 1 cut(s) 2532
BslI CCNNNNNNNGG 5 cut(s) 70, 2109, 2190, 2623, 2639
BsmAI GTCTC 3 cut(s) 99, 528, 656
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 2532
BsnI GGCC 4 cut(s) 41, 88, 1226, 1617
BsoBI CYCGRG 1 cut(s) 2866
Bsp1286I GDGCHC 7 cut(s) 401, 485, 813, 2087, 2413, 2531, 2919
Bsp1720I GCTNAGC 1 cut(s) 1635
Bsp19I CCATGG 1 cut(s) 83
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 6 cut(s) 38, 404, 687, 1785, 1970, 2072
BspANI GGCC 4 cut(s) 41, 88, 1226, 1617
BspCNI CTCAG 7 cut(s) 109, 340, 1423, 1581, 1627, 1873, 2517
BspDI ATCGAT 1 cut(s) 2469
BspFNI CGCG 3 cut(s) 38, 261, 1502
BspHI TCATGA 1 cut(s) 2131
BspLI GGNNCC 3 cut(s) 744, 1646, 1968
BspMAI CTGCAG 1 cut(s) 1868
BspPI GGATC 7 cut(s) 102, 166, 380, 1961, 1974, 2018, 2330
BspQI GCTCTTC 2 cut(s) 26, 856
BspTI CTTAAG 2 cut(s) 1577, 2888
BsrDI GCAATG 2 cut(s) 178, 1186
BsrI ACTGG 6 cut(s) 218, 416, 791, 1762, 1852, 2976
BssECI CCNNGG 3 cut(s) 83, 554, 651
BssNAI GTATAC 2 cut(s) 1236, 2901
BssSI CACGAG 1 cut(s) 1743
BssT1I CCWWGG 1 cut(s) 83
Bst1107I GTATAC 2 cut(s) 1236, 2901
Bst2BI CACGAG 1 cut(s) 1743
Bst2UI CCWGG 2 cut(s) 555, 771
Bst4CI ACNGT 9 cut(s) 143, 445, 643, 670, 756, 837, 902, 1425, 1528
Bst6I CTCTTC 6 cut(s) 26, 413, 513, 856, 1920, 2316
BstAFI CTTAAG 2 cut(s) 1577, 2888
BstAPI GCANNNNNTGC 2 cut(s) 1181, 1748
BstC8I GCNNGC 7 cut(s) 747, 1177, 1705, 1783, 2234, 2244, 2601
BstDEI CTNAG 9 cut(s) 96, 348, 1410, 1589, 1635, 1725, 1881, 2364, 2504
BstDSI CCRYGG 1 cut(s) 83
BstEII GGTNACC 1 cut(s) 1007
BstENI CCTNNNNNAGG 1 cut(s) 2188
BstF5I GGATG 6 cut(s) 60, 628, 1130, 1213, 2287, 2534
BstFNI CGCG 3 cut(s) 38, 261, 1502
BstH2I RGCGCY 1 cut(s) 1881
BstHHI GCGC 3 cut(s) 38, 1502, 1880
BstMAI GTCTC 3 cut(s) 99, 528, 656
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 7 cut(s) 33, 1181, 1713, 1730, 1739, 1745, 1748
BstNI CCWGG 2 cut(s) 555, 771
BstNSI RCATGY 3 cut(s) 1144, 1379, 1785
BstPAI GACNNNNGTC 1 cut(s) 668
BstPI GGTNACC 1 cut(s) 1007
BstSCI CCNGG 3 cut(s) 553, 769, 2615
BstSFI CTRYAG 1 cut(s) 1864
BstSLI GKGCMC 2 cut(s) 2413, 2531
BstUI CGCG 3 cut(s) 38, 261, 1502
BstV2I GAAGAC 2 cut(s) 960, 2546
BstX2I RGATCY 5 cut(s) 107, 1084, 1966, 2010, 2335
BstXI CCANNNNNNTGG 1 cut(s) 2983
BstYI RGATCY 5 cut(s) 107, 1084, 1966, 2010, 2335
BstZ17I GTATAC 2 cut(s) 1236, 2901
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 2469
BsuRI GGCC 4 cut(s) 41, 88, 1226, 1617
BsuTUI ATCGAT 1 cut(s) 2469
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 6 cut(s) 60, 628, 1130, 1213, 2287, 2534
BtsIMutI CAGTG 8 cut(s) 137, 573, 639, 798, 833, 1755, 2512, 2889
BtuMI TCGCGA 1 cut(s) 261
Cac8I GCNNGC 7 cut(s) 747, 1177, 1705, 1783, 2234, 2244, 2601
CaiI CAGNNNCTG 3 cut(s) 560, 770, 2339
CciI TCATGA 1 cut(s) 2131
CfoI GCGC 3 cut(s) 38, 1502, 1880
Cfr13I GGNCC 1 cut(s) 87
ClaI ATCGAT 1 cut(s) 2469
CseI GACGC 1 cut(s) 954
DdeI CTNAG 9 cut(s) 96, 348, 1410, 1589, 1635, 1725, 1881, 2364, 2504
DraI TTTAAA 2 cut(s) 1893, 2542
DrdI GACNNNNNNGTC 1 cut(s) 1103
DseDI GACNNNNNNGTC 1 cut(s) 1103
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 6 cut(s) 26, 413, 513, 856, 1920, 2316
EarI CTCTTC 6 cut(s) 26, 413, 513, 856, 1920, 2316
Ecl136II GAGCTC 2 cut(s) 483, 2917
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 3 cut(s) 485, 2087, 2919
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 2 cut(s) 483, 2917
Eco57I CTGAAG 3 cut(s) 113, 1143, 2340
Eco88I CYCGRG 1 cut(s) 2866
Eco91I GGTNACC 1 cut(s) 1007
EcoICRI GAGCTC 2 cut(s) 483, 2917
EcoNI CCTNNNNNAGG 1 cut(s) 2188
EcoO65I GGTNACC 1 cut(s) 1007
EcoRI GAATTC 5 cut(s) 868, 1032, 1822, 2743, 3022
EcoRII CCWGG 2 cut(s) 553, 769
EcoT14I CCWWGG 1 cut(s) 83
EcoT38I GRGCYC 3 cut(s) 485, 2087, 2919
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 99
FalI AAGNNNNNCTT 6 cut(s) 266, 298, 491, 523, 2381, 2413
FaqI GGGAC 1 cut(s) 2532
FbaI TGATCA 3 cut(s) 145, 2509, 2652
FblI GTMKAC 2 cut(s) 1235, 2900
FokI GGATG 6 cut(s) 47, 615, 1117, 1220, 2294, 2521
FriOI GRGCYC 3 cut(s) 485, 2087, 2919
FspBI CTAG 4 cut(s) 684, 726, 1686, 2442
GlaI GCGC 3 cut(s) 37, 1501, 1879
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 3 cut(s) 469, 774, 2799
HaeII RGCGCY 1 cut(s) 1881
HaeIII GGCC 4 cut(s) 41, 88, 1226, 1617
HapII CCGG 1 cut(s) 2617
HgaI GACGC 1 cut(s) 954
HhaI GCGC 3 cut(s) 38, 1502, 1880
Hin6I GCGC 3 cut(s) 36, 1500, 1878
HinP1I GCGC 3 cut(s) 36, 1500, 1878
HincII GTYRAC 3 cut(s) 332, 1552, 1806
HindII GTYRAC 3 cut(s) 332, 1552, 1806
HindIII AAGCTT 2 cut(s) 1714, 2544
HpaII CCGG 1 cut(s) 2617
HphI GGTGA 9 cut(s) 155, 191, 672, 701, 796, 937, 963, 2799, 2925
Hpy166II GTNNAC 8 cut(s) 332, 476, 646, 1236, 1552, 1806, 2411, 2901
Hpy188I TCNGA 5 cut(s) 1413, 1540, 2347, 2749, 3021
Hpy8I GTNNAC 8 cut(s) 332, 476, 646, 1236, 1552, 1806, 2411, 2901
HpyAV CCTTC 8 cut(s) 1118, 1723, 1997, 2074, 2103, 2161, 3004, 3052
HpyCH4III ACNGT 9 cut(s) 143, 445, 643, 670, 756, 837, 902, 1425, 1528
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 1181, 1713, 1730, 1739, 1745, 1748
HpyF3I CTNAG 9 cut(s) 96, 348, 1410, 1589, 1635, 1725, 1881, 2364, 2504
HspAI GCGC 3 cut(s) 36, 1500, 1878
Ksp22I TGATCA 3 cut(s) 145, 2509, 2652
LguI GCTCTTC 2 cut(s) 26, 856
LmnI GCTCC 7 cut(s) 488, 742, 1644, 1672, 1721, 2155, 2260
LweI GCATC 9 cut(s) 637, 1264, 1465, 2170, 2316, 2470, 2475, 2571, 2654
MaeI CTAG 4 cut(s) 684, 726, 1686, 2442
MaeIII GTNAC 7 cut(s) 220, 784, 1007, 1109, 1392, 2141, 2570
MfeI CAATTG 3 cut(s) 1566, 1827, 1933
MflI RGATCY 5 cut(s) 107, 1084, 1966, 2010, 2335
MhlI GDGCHC 7 cut(s) 401, 485, 813, 2087, 2413, 2531, 2919
MlyI GAGTC 6 cut(s) 212, 530, 781, 2039, 2047, 2756
MmeI TCCRAC 6 cut(s) 1315, 1988, 2194, 2370, 2907, 2999
MroXI GAANNNNTTC 2 cut(s) 549, 2171
MslI CAYNNNNRTG 6 cut(s) 65, 782, 816, 1074, 2223, 3004
MspA1I CMGCKG 4 cut(s) 406, 2072, 2384, 2819
MspCI CTTAAG 2 cut(s) 1577, 2888
MspI CCGG 1 cut(s) 2617
MspR9I CCNGG 3 cut(s) 555, 771, 2617
MunI CAATTG 3 cut(s) 1566, 1827, 1933
MvaI CCWGG 2 cut(s) 555, 771
MvnI CGCG 3 cut(s) 38, 261, 1502
MwoI GCNNNNNNNGC 7 cut(s) 33, 1181, 1713, 1730, 1739, 1745, 1748
NciI CCSGG 1 cut(s) 2617
NcoI CCATGG 1 cut(s) 83
NlaIV GGNNCC 3 cut(s) 744, 1646, 1968
NmeAIII GCCGAG 2 cut(s) 632, 1654
NmuCI GTSAC 3 cut(s) 220, 784, 2570
NruI TCGCGA 1 cut(s) 261
NspI RCATGY 3 cut(s) 1144, 1379, 1785
OliI CACNNNNGTG 2 cut(s) 782, 3004
PaeI GCATGC 1 cut(s) 1785
PaeR7I CTCGAG 1 cut(s) 2866
PagI TCATGA 1 cut(s) 2131
PciI ACATGT 1 cut(s) 1375
PciSI GCTCTTC 2 cut(s) 26, 856
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 258
PdmI GAANNNNTTC 2 cut(s) 549, 2171
PfeI GAWTC 9 cut(s) 545, 707, 1001, 1187, 1559, 1660, 1689, 2360, 2930
PleI GAGTC 6 cut(s) 211, 529, 781, 2038, 2047, 2756
PpsI GAGTC 6 cut(s) 211, 529, 781, 2038, 2047, 2756
PscI ACATGT 1 cut(s) 1375
PshAI GACNNNNGTC 1 cut(s) 668
Psp124BI GAGCTC 2 cut(s) 485, 2919
Psp6I CCWGG 2 cut(s) 553, 769
PspEI GGTNACC 1 cut(s) 1007
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 2 cut(s) 553, 769
PspN4I GGNNCC 3 cut(s) 744, 1646, 1968
PspPI GGNCC 1 cut(s) 87
PstI CTGCAG 1 cut(s) 1868
PstNI CAGNNNCTG 3 cut(s) 560, 770, 2339
PsuI RGATCY 5 cut(s) 107, 1084, 1966, 2010, 2335
PvuII CAGCTG 2 cut(s) 2384, 2819
RruI TCGCGA 1 cut(s) 261
RseI CAYNNNNRTG 6 cut(s) 65, 782, 816, 1074, 2223, 3004
SacI GAGCTC 2 cut(s) 485, 2919
SapI GCTCTTC 2 cut(s) 26, 856
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 6 cut(s) 212, 530, 781, 2039, 2047, 2756
ScrFI CCNGG 3 cut(s) 555, 771, 2617
SduI GDGCHC 7 cut(s) 401, 485, 813, 2087, 2413, 2531, 2919
SfaNI GCATC 9 cut(s) 637, 1264, 1465, 2170, 2316, 2470, 2475, 2571, 2654
SfcI CTRYAG 1 cut(s) 1864
Sfr274I CTCGAG 1 cut(s) 2866
SlaI CTCGAG 1 cut(s) 2866
SmiMI CAYNNNNRTG 6 cut(s) 65, 782, 816, 1074, 2223, 3004
SmlI CTYRAG 8 cut(s) 1080, 1135, 1470, 1577, 2086, 2092, 2866, 2888
SmoI CTYRAG 8 cut(s) 1080, 1135, 1470, 1577, 2086, 2092, 2866, 2888
SphI GCATGC 1 cut(s) 1785
SsiI CCGC 6 cut(s) 38, 404, 687, 1785, 1970, 2072
SspI AATATT 1 cut(s) 1102
SspMI CTAG 4 cut(s) 684, 726, 1686, 2442
SstI GAGCTC 2 cut(s) 485, 2919
StyD4I CCNGG 3 cut(s) 553, 769, 2615
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 9 cut(s) 143, 445, 643, 670, 756, 837, 902, 1425, 1528
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 9 cut(s) 545, 707, 1001, 1187, 1559, 1660, 1689, 2360, 2930
TscAI CASTG 8 cut(s) 137, 580, 646, 798, 840, 1762, 2512, 2889
TseFI GTSAC 3 cut(s) 220, 784, 2570
Tsp45I GTSAC 3 cut(s) 220, 784, 2570
TspGWI ACGGA 5 cut(s) 100, 109, 1097, 2540, 3035
TspRI CASTG 8 cut(s) 137, 580, 646, 798, 840, 1762, 2512, 2889
Vha464I CTTAAG 2 cut(s) 1577, 2888
VneI GTGCAC 1 cut(s) 2409
XagI CCTNNNNNAGG 1 cut(s) 2188
XapI RAATTY 6 cut(s) 194, 868, 1032, 1822, 2743, 3022
XbaI TCTAGA 2 cut(s) 1685, 2441
XceI RCATGY 3 cut(s) 1144, 1379, 1785
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XhoI CTCGAG 1 cut(s) 2866
XmiI GTMKAC 2 cut(s) 1235, 2900
XmnI GAANNNNTTC 2 cut(s) 549, 2171
XspI CTAG 4 cut(s) 684, 726, 1686, 2442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.