pycom13g10000

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003470
Physical Location & Seq
Forward (+)
6679987 .. 6684837
4851 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10000.1

Sequence Viewer

Length: 3144 bp
ATGGTTCTTGGGATCAGAGCAAAGAGCAGGAAGAGCGTGGCCGTTGATGTTGATTACCTCATCCATGTGCAGGAGTTAAAGCCATGGCCTTCATCTCAGACTTTGAAGTCTGTTCAATCTGTCCTGCTTCAATGGGAAAATGGTGATCAGGTTTCTGGATCGTGTATTTGCAATGTTGGTGATGGGAAGGTCGAATTCAGCGAGTCTTTCACGCTTCCAGTCACTTTATATAAGGAGAAATCAAGAAAAAGTGCAGTTCGCGAGTCTTACCTGAAGAACAACTTAGAGTTTTACTTGTATGAACCTCGAAAGGACAAGGGAGTTAAAGGTCAACTCTTGGGGTCAGCTGTGATCAATCTTGCTGACTACGGGATCATTGTGGAAACCAGAAATGTAAGCACTCCTCTGAACTGGAAGAAAAGTTTTAAGAGTTCAGCTCAACCGGTTCTTTATGTTAGTATTCAGCCATGTGGTAAATCTGGCTCCAGCTTGACACCAAATAGTGGCTTGTCCAGAGAAGTGTCTCTGGAAAATGATGGAACTGAATCTGTTCCAGAGTCTGCGAATGATGGTAATGATGAGATTGCCTCTTTTACTGATGACGATGACGATGTTTCCTCGCATTCGTCACGTACTGTAAACTCCTCGGCTATTGAGACAACAGTGAATTCATCACCTAGCGGTGAAAAGAATGGACTAGAATCAACAATCAGTAAAAGGACTTATGGGGAGCCTGCTGTACAGTCAATGGCAGCACCTGCAAGCACAGGGGTAACTCCAGTGGCTAAAGCATTCAAACACCAAAATGGAAGTTCATCTCCCCTGTCATCAACAGGTTCTTCCTCCAGTCAGCTGAACTCTTCAAATGATCCTGCTTTCTTGCCTAACAGTTCAAAGGAGAGCTCAATGCCAATACTTAAAAAATCACTCACTCACTCTGTTCATTCAGCGTCTTCATCCTTTGGCTATCAAGATAGCCATCAAGAATCTGGGTACCATAATTTTAAGGACAACAGAATTCACATAACTCCATCTAACGGGAGTGCCAGAATGCATGAAAATGCTCAAGAACTTATCACGGGAAATATTGTCAGTACCCATGCTGCTGAAGGTGCATCCTCGAGTATGTCTATTCAGGAGGACACAAACTCCACATTTGCAAGCAACGCAGATTCACAAGCCCTTCGAGAGGATGGCCATCTCCTAATGGCGAATCAATATTCTTTTGATGACAAATTAGCTTCCAGATTTTCACAAGATCCTACCAGAAAGCAAGTCAGGTTAAGGAGTGAAACTTTTACACCTGGCAGGAAAGTTGTTGGAGTTCAGGGAAGTAAGGCTAAAAGTAATGAACTAAAGCATGTAAAGTCTGTGCAGTTACCTTTAGTATCAGCTCAGAACAATAGACAGTCAAGCAATAATGAGTTTCTCGAAAAGTCAAGGGAAGCTGAAACTCCTGAAGATGCTTCTGTTCACGGTTGGATTAGCTCAACAAGTGAAAGTGAACAAAAAATAGTCAGCTTTTCTGATAGCAAAGTTGACTTGGAGTCCACAATTGAATTGCTCAAAGAAGAACTGAGAGAAGCTGCTGCTGTTGAGGTTGGCCTTTACTCTGTTGCCGCTGAGCATGGGAGCCACACTAATAAGATTCACGCTCCTGCTCGGCGACTTTCTAGGTTCTATTTTCATGCTTGCAAAACAAGCTCGCGAGCTAAGAAGGCAAATGCAGCAAGAGTTGCTATCACTGGACTAATCTTGGTTTCTAAAGCATGCGGAAATGATGTTGCGAGGTTGACATTCTGGTTGTCAAATTCAATTGTGTTGAGAGCTATCATCAGCCAGACCTTATCCTGCAATAAAATTAGTGCTGAGGGGGTTTTGAAACCAAAGAATGGGTTTCCTCCTCACAAGGAAGAGAACAATAGCACTGTTGAAAGTTTCGATGATTGGGAAGATCCACAGATATTTATGGCAGCTTTGGAAAAGTTTGAAGGATGGATTTTCTCCCGAATTGTTGAGTCTGTATGGTGGCAGAATATGACTCCATATATGCAGTCAGCAGCTGCAAAGGGTTCAAGCTCAAGGAAAGCCAATGGAAGGAGGCATGGTTTGGGTAGTCATGAGCAGGGTAACTTTTCTATGGAACTGTGGAAGAAGGCTTTCAAAGATGTCTGTGAAAGGCTTTGTCCTGCAAGAGCTGGTGGACATGAGTGTGGCTGCTTGCCTGTGCTGGCTCGTTTGGTAATGGAGCAATTAGTGGATAGATTGGATGTGGCTATGTTCAATGCTATTCTTCGTGAAAATGCTGAAGAGATGCCGATCGATCCTGTGTCCGACCCCATAAGTGATTCTAAGGTTCTCCCTATTCCAGCTGGAAATTTGAGTTTTGGGGCTGGTGTACAGCTAAAAAATGCGATTGGAAGCTGGTCCAGATGGCTTACTGATCTATTTGGCATCGATGATACTGATGCTCCTGATGATAATACTGAACTCAGTGATCACAAGGGACAAGAGCGTGAAACATCCTTTAGAGCTTTCCGTCTTCTTAATGCTTTGAGTGACCTCACGATGCTTCCATTTGATATGCTTCCAGATAAATCCACCCGGAAAGAGGTATGCCCTACATTTGGTGCATCATTGATCAAGAGGGTTCTTTACAATTTTGTTCCGGATGAGTTTTGCCCAGACCCAATTCCCGAAGCAGTTTTTGAGGCTCTTGATTATGAGGAGGATTCGGAAGCTGAAGCAGAGTCTGCTGCAAGTTTTCCATGCAACGCAAATCCTACAGTGTATTCACCACCTCCAGCTGCTTCACTACTAGGCATTATAGGAGATGTGGGAAGTCCAACTTTCTCGAGGAGTGGGTCATCAGTGCTCAAGAAATCGTATACAAGTGATGATGAGCTTGATGAGTTGGATTCACCGATGACTTCAATCATCATAGACAATTCCCCGTTTTCTCCCAGTCCATTGGCAGCCTCAACGCCGAAGTGGAAGGGTGGTCGGAAAGCTGTCAGGTATCAACTCCTCCGTGAAGTTTGGAAGGATAGCGATCGATCTTCCCTTTCATGTACGTATCTGATATACTCGGGAGGGTTGTTTAATTTGGGGGGCTCTCTTTACTTCACGCTTGTACATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1048

Amino Acids

114.33

Weight (kDa)

5.71

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 14 - 155 7.9e-14 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 766
AasI GACNNNNNNGTC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 766
Acc65I GGTACC 1 cut(s) 993
AccB1I GGYRCC 1 cut(s) 993
AccB7I CCANNNNNTGG 2 cut(s) 503, 1892
AccI GTMKAC 1 cut(s) 2888
AccII CGCG 2 cut(s) 261, 1708
AccIII TCCGGA 1 cut(s) 2668
AciI CCGC 3 cut(s) 681, 1620, 1773
AclWI GGATC 7 cut(s) 20, 166, 380, 863, 1253, 1949, 2318
AcoI YGGCCR 2 cut(s) 39, 1195
AcsI RAATTY 5 cut(s) 194, 667, 1017, 1810, 2377
AcuI CTGAAG 5 cut(s) 293, 1128, 1479, 2328, 2763
AfaI GTAC 7 cut(s) 634, 741, 995, 1096, 2400, 3073, 3135
AfiI CCNNNNNNNGG 8 cut(s) 70, 503, 1037, 1189, 1892, 2097, 2611, 2627
AgeI ACCGGT 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 1546
AjnI CCWGG 1 cut(s) 1303
Alw21I GWGCWC 2 cut(s) 905, 2877
Alw26I GTCTC 2 cut(s) 528, 650
AlwI GGATC 7 cut(s) 20, 166, 380, 863, 1253, 1949, 2318
AlwNI CAGNNNCTG 4 cut(s) 560, 758, 2063, 2753
Ama87I CYCGRG 3 cut(s) 1120, 2854, 3088
Aor13HI TCCGGA 1 cut(s) 2668
AoxI GGCC 4 cut(s) 39, 86, 1195, 1603
ApoI RAATTY 5 cut(s) 194, 667, 1017, 1810, 2377
AsiGI ACCGGT 1 cut(s) 442
Asp700I GAANNNNTTC 2 cut(s) 549, 2159
Asp718I GGTACC 1 cut(s) 993
AspS9I GGNCC 1 cut(s) 2427
AsuC2I CCSGG 1 cut(s) 2605
AsuHPI GGTGA 6 cut(s) 155, 191, 666, 695, 2787, 2913
AvaI CYCGRG 3 cut(s) 1120, 2854, 3088
AvaII GGWCC 1 cut(s) 2427
BalI TGGCCA 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 993
BanII GRGCYC 2 cut(s) 905, 3116
BbsI GAAGAC 2 cut(s) 945, 2534
Bbv12I GWGCWC 2 cut(s) 905, 2877
BbvCI CCTCAGC 1 cut(s) 1869
BccI CCATC 9 cut(s) 176, 530, 563, 987, 1039, 1187, 1206, 1989, 2427
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 1305
BclI TGATCA 4 cut(s) 145, 351, 2497, 2640
BcnI CCSGG 1 cut(s) 2605
BcoDI GTCTC 2 cut(s) 528, 650
BfaI CTAG 4 cut(s) 678, 698, 1674, 2819
BfmI CTRYAG 1 cut(s) 2784
BfuAI ACCTGC 1 cut(s) 766
BlpI GCTNAGC 1 cut(s) 1623
Bme1390I CCNGG 2 cut(s) 1305, 2605
Bme18I GGWCC 1 cut(s) 2427
BmeRI GACNNNNNGTC 1 cut(s) 1546
BmeT110I CYCGRG 3 cut(s) 1120, 2854, 3088
BmgT120I GGNCC 1 cut(s) 2427
BmiI GGNNCC 4 cut(s) 484, 732, 995, 1634
BmrFI CCNGG 2 cut(s) 1305, 2605
BmrI ACTGGG 1 cut(s) 2958
BmsI GCATC 7 cut(s) 1124, 1453, 2304, 2458, 2463, 2559, 2642
BmuI ACTGGG 1 cut(s) 2958
BpiI GAAGAC 2 cut(s) 945, 2534
BplI GAGNNNNNCTC 4 cut(s) 421, 453, 572, 604
BpmI CTGGAG 4 cut(s) 469, 762, 829, 2787
Bpu10I CCTNAGC 1 cut(s) 1869
Bpu1102I GCTNAGC 1 cut(s) 1623
BpuEI CTTGAG 3 cut(s) 1050, 2065, 2861
BpuMI CCSGG 1 cut(s) 2605
Bsa29I ATCGAT 3 cut(s) 2322, 2457, 3055
BsaAI YACGTR 2 cut(s) 632, 3075
BsaBI GATNNNNATC 4 cut(s) 978, 1197, 2318, 3051
BsaJI CCNNGG 2 cut(s) 83, 645
BsaWI WCCGGW 2 cut(s) 442, 2668
BsaXI ACNNNNNCTCC 6 cut(s) 1133, 1163, 2092, 2122, 2455, 2485
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 503, 1037, 1189, 1892, 2097, 2611, 2627
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 6 cut(s) 218, 416, 779, 846, 1750, 2964
Bse3DI GCAATG 1 cut(s) 178
Bse8I GATNNNNATC 4 cut(s) 978, 1197, 2318, 3051
BseAI TCCGGA 1 cut(s) 2668
BseBI CCWGG 1 cut(s) 1305
BseCI ATCGAT 3 cut(s) 2322, 2457, 3055
BseDI CCNNGG 2 cut(s) 83, 645
BseGI GGATG 8 cut(s) 60, 956, 1115, 1198, 2000, 2275, 2522, 2677
BseJI GATNNNNATC 4 cut(s) 978, 1197, 2318, 3051
BseLI CCNNNNNNNGG 8 cut(s) 70, 503, 1037, 1189, 1892, 2097, 2611, 2627
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 6 cut(s) 110, 1409, 1568, 1614, 1860, 2506
BseNI ACTGG 6 cut(s) 218, 416, 779, 846, 1750, 2964
BseRI GAGGAG 6 cut(s) 393, 634, 1893, 2741, 2872, 3017
BsgI GTGCAG 3 cut(s) 89, 273, 1394
Bsh1236I CGCG 2 cut(s) 261, 1708
Bsh1285I CGRYCG 2 cut(s) 2322, 3055
BshFI GGCC 4 cut(s) 41, 88, 1197, 1605
BshNI GGYRCC 1 cut(s) 993
BshTI ACCGGT 1 cut(s) 442
BshVI ATCGAT 3 cut(s) 2322, 2457, 3055
BsiEI CGRYCG 2 cut(s) 2322, 3055
BsiHKAI GWGCWC 2 cut(s) 905, 2877
BsiHKCI CYCGRG 3 cut(s) 1120, 2854, 3088
BsiSI CCGG 3 cut(s) 443, 2605, 2669
BslFI GGGAC 1 cut(s) 2520
BslI CCNNNNNNNGG 8 cut(s) 70, 503, 1037, 1189, 1892, 2097, 2611, 2627
BsmAI GTCTC 2 cut(s) 528, 650
BsmFI GGGAC 1 cut(s) 2520
BsmI GAATGC 3 cut(s) 622, 791, 1056
BsnI GGCC 4 cut(s) 41, 88, 1197, 1605
BsoBI CYCGRG 3 cut(s) 1120, 2854, 3088
Bsp1286I GDGCHC 3 cut(s) 905, 2877, 3116
Bsp13I TCCGGA 1 cut(s) 2668
Bsp1407I TGTACA 3 cut(s) 739, 2398, 3133
Bsp1720I GCTNAGC 1 cut(s) 1623
Bsp19I CCATGG 1 cut(s) 83
Bsp68I TCGCGA 2 cut(s) 261, 1708
BspACI CCGC 3 cut(s) 681, 1620, 1773
BspANI GGCC 4 cut(s) 41, 88, 1197, 1605
BspCNI CTCAG 6 cut(s) 109, 1408, 1569, 1615, 1861, 2505
BspDI ATCGAT 3 cut(s) 2322, 2457, 3055
BspEI TCCGGA 1 cut(s) 2668
BspFNI CGCG 2 cut(s) 261, 1708
BspHI TCATGA 1 cut(s) 2119
BspLI GGNNCC 4 cut(s) 484, 732, 995, 1634
BspMI ACCTGC 1 cut(s) 766
BspPI GGATC 7 cut(s) 20, 166, 380, 863, 1253, 1949, 2318
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 993
BsrDI GCAATG 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 442
BsrGI TGTACA 3 cut(s) 739, 2398, 3133
BsrI ACTGG 6 cut(s) 218, 416, 779, 846, 1750, 2964
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 2 cut(s) 83, 645
BssNAI GTATAC 1 cut(s) 2889
BssT1I CCWWGG 1 cut(s) 83
Bst1107I GTATAC 1 cut(s) 2889
Bst2UI CCWGG 1 cut(s) 1305
Bst4CI ACNGT 9 cut(s) 637, 664, 744, 890, 1410, 1478, 1930, 2148, 2788
Bst6I CTCTTC 4 cut(s) 26, 865, 1908, 2304
BstAPI GCANNNNNTGC 3 cut(s) 758, 1736, 2753
BstAUI TGTACA 3 cut(s) 739, 2398, 3133
BstBAI YACGTR 2 cut(s) 632, 3075
BstC8I GCNNGC 9 cut(s) 735, 763, 1162, 1693, 1706, 1710, 1771, 2222, 2232
BstDEI CTNAG 9 cut(s) 96, 283, 1395, 1577, 1623, 1713, 1869, 2352, 2492
BstDSI CCRYGG 1 cut(s) 83
BstENI CCTNNNNNAGG 1 cut(s) 1187
BstF5I GGATG 8 cut(s) 60, 956, 1115, 1198, 2000, 2275, 2522, 2677
BstFNI CGCG 2 cut(s) 261, 1708
BstMAI GTCTC 2 cut(s) 528, 650
BstMCI CGRYCG 2 cut(s) 2322, 3055
BstMWI GCNNNNNNNGC 9 cut(s) 33, 758, 1112, 1166, 1701, 1718, 1727, 1736, 2753
BstNI CCWGG 1 cut(s) 1305
BstNSI RCATGY 2 cut(s) 1364, 1773
BstSCI CCNGG 2 cut(s) 1303, 2603
BstSFI CTRYAG 1 cut(s) 2784
BstSNI TACGTA 1 cut(s) 3075
BstUI CGCG 2 cut(s) 261, 1708
BstV2I GAAGAC 2 cut(s) 945, 2534
BstX2I RGATCY 2 cut(s) 1258, 1954
BstXI CCANNNNNNTGG 1 cut(s) 2971
BstYI RGATCY 2 cut(s) 1258, 1954
BstZ17I GTATAC 1 cut(s) 2889
Bsu15I ATCGAT 3 cut(s) 2322, 2457, 3055
BsuRI GGCC 4 cut(s) 41, 88, 1197, 1605
BsuTUI ATCGAT 3 cut(s) 2322, 2457, 3055
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 8 cut(s) 60, 956, 1115, 1198, 2000, 2275, 2522, 2677
BtsIMutI CAGTG 7 cut(s) 669, 786, 1743, 1926, 2500, 2793, 2877
BtuMI TCGCGA 2 cut(s) 261, 1708
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 9 cut(s) 735, 763, 1162, 1693, 1706, 1710, 1771, 2222, 2232
CaiI CAGNNNCTG 4 cut(s) 560, 758, 2063, 2753
CciI TCATGA 1 cut(s) 2119
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 2427
ClaI ATCGAT 3 cut(s) 2322, 2457, 3055
CseI GACGC 1 cut(s) 939
Csp6I GTAC 7 cut(s) 633, 740, 994, 1095, 2399, 3072, 3134
CspAI ACCGGT 1 cut(s) 442
CviQI GTAC 7 cut(s) 633, 740, 994, 1095, 2399, 3072, 3134
DdeI CTNAG 9 cut(s) 96, 283, 1395, 1577, 1623, 1713, 1869, 2352, 2492
DrdI GACNNNNNNGTC 1 cut(s) 106
DriI GACNNNNNGTC 1 cut(s) 1546
DseDI GACNNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 2 cut(s) 39, 1195
Eam1104I CTCTTC 4 cut(s) 26, 865, 1908, 2304
Eam1105I GACNNNNNGTC 1 cut(s) 1546
EarI CTCTTC 4 cut(s) 26, 865, 1908, 2304
Ecl136II GAGCTC 1 cut(s) 903
Eco105I TACGTA 1 cut(s) 3075
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 2 cut(s) 905, 3116
Eco47I GGWCC 1 cut(s) 2427
Eco53kI GAGCTC 1 cut(s) 903
Eco57I CTGAAG 5 cut(s) 293, 1128, 1479, 2328, 2763
Eco88I CYCGRG 3 cut(s) 1120, 2854, 3088
EcoICRI GAGCTC 1 cut(s) 903
EcoNI CCTNNNNNAGG 1 cut(s) 1187
EcoRI GAATTC 3 cut(s) 194, 667, 1017
EcoRII CCWGG 1 cut(s) 1303
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 1056
EcoT38I GRGCYC 2 cut(s) 905, 3116
ErhI CCWWGG 1 cut(s) 83
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 2833, 2865
FaqI GGGAC 1 cut(s) 2520
FbaI TGATCA 4 cut(s) 145, 351, 2497, 2640
FblI GTMKAC 1 cut(s) 2888
FokI GGATG 8 cut(s) 47, 943, 1102, 1205, 2007, 2282, 2509, 2684
FriOI GRGCYC 2 cut(s) 905, 3116
FspBI CTAG 4 cut(s) 678, 698, 1674, 2819
GsuI CTGGAG 4 cut(s) 469, 762, 829, 2787
HaeIII GGCC 4 cut(s) 41, 88, 1197, 1605
HapII CCGG 3 cut(s) 443, 2605, 2669
HgaI GACGC 1 cut(s) 939
HincII GTYRAC 3 cut(s) 332, 1540, 1794
HindII GTYRAC 3 cut(s) 332, 1540, 1794
HpaII CCGG 3 cut(s) 443, 2605, 2669
HphI GGTGA 6 cut(s) 155, 191, 666, 695, 2787, 2913
Hpy188I TCNGA 9 cut(s) 17, 99, 408, 1398, 1528, 2335, 2737, 3006, 3081
HpyCH4III ACNGT 9 cut(s) 637, 664, 744, 890, 1410, 1478, 1930, 2148, 2788
HpyCH4IV ACGT 2 cut(s) 631, 3074
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 758, 1112, 1166, 1701, 1718, 1727, 1736, 2753
HpyF3I CTNAG 9 cut(s) 96, 283, 1395, 1577, 1623, 1713, 1869, 2352, 2492
HpySE526I ACGT 2 cut(s) 631, 3074
Kpn2I TCCGGA 1 cut(s) 2668
KpnI GGTACC 1 cut(s) 997
Ksp22I TGATCA 4 cut(s) 145, 351, 2497, 2640
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 6 cut(s) 488, 730, 1632, 1660, 2248, 2476
LweI GCATC 7 cut(s) 1124, 1453, 2304, 2458, 2463, 2559, 2642
MaeI CTAG 4 cut(s) 678, 698, 1674, 2819
MaeII ACGT 2 cut(s) 631, 3074
MaeIII GTNAC 6 cut(s) 220, 627, 772, 1377, 2129, 2558
MfeI CAATTG 2 cut(s) 1554, 1815
MflI RGATCY 2 cut(s) 1258, 1954
MhlI GDGCHC 3 cut(s) 905, 2877, 3116
MlsI TGGCCA 1 cut(s) 1197
MluNI TGGCCA 1 cut(s) 1197
MlyI GAGTC 7 cut(s) 212, 272, 566, 1556, 2027, 2035, 2759
MmeI TCCRAC 6 cut(s) 1300, 1460, 2358, 2870, 2895, 2984
Mox20I TGGCCA 1 cut(s) 1197
Mph1103I ATGCAT 1 cut(s) 1056
MroI TCCGGA 1 cut(s) 2668
MroXI GAANNNNTTC 2 cut(s) 549, 2159
MscI TGGCCA 1 cut(s) 1197
MseI TTAA 8 cut(s) 77, 324, 426, 918, 1005, 1283, 2547, 3102
MslI CAYNNNNRTG 4 cut(s) 65, 804, 1059, 2211
Msp20I TGGCCA 1 cut(s) 1197
MspA1I CMGCKG 6 cut(s) 347, 853, 1622, 2063, 2372, 2807
MspI CCGG 3 cut(s) 443, 2605, 2669
MspR9I CCNGG 2 cut(s) 1305, 2605
MunI CAATTG 2 cut(s) 1554, 1815
Mva1269I GAATGC 3 cut(s) 622, 791, 1056
MvaI CCWGG 1 cut(s) 1305
MvnI CGCG 2 cut(s) 261, 1708
MwoI GCNNNNNNNGC 9 cut(s) 33, 758, 1112, 1166, 1701, 1718, 1727, 1736, 2753
NciI CCSGG 1 cut(s) 2605
NcoI CCATGG 1 cut(s) 83
NlaIV GGNNCC 4 cut(s) 484, 732, 995, 1634
NmeAIII GCCGAG 2 cut(s) 626, 1642
NmuCI GTSAC 3 cut(s) 220, 627, 2558
NruI TCGCGA 2 cut(s) 261, 1708
NsiI ATGCAT 1 cut(s) 1056
NspI RCATGY 2 cut(s) 1364, 1773
PaeI GCATGC 1 cut(s) 1773
PaeR7I CTCGAG 2 cut(s) 1120, 2854
PagI TCATGA 1 cut(s) 2119
PaqCI CACCTGC 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 3 cut(s) 622, 791, 1056
PdmI GAANNNNTTC 2 cut(s) 549, 2159
PfeI GAWTC 9 cut(s) 545, 701, 986, 1172, 1213, 1648, 2348, 2732, 2918
PflMI CCANNNNNTGG 2 cut(s) 503, 1892
PinAI ACCGGT 1 cut(s) 442
Ple19I CGATCG 2 cut(s) 2322, 3055
PleI GAGTC 7 cut(s) 211, 271, 565, 1555, 2026, 2035, 2758
PpsI GAGTC 7 cut(s) 211, 271, 565, 1555, 2026, 2035, 2758
Ppu21I YACGTR 2 cut(s) 632, 3075
Psp124BI GAGCTC 1 cut(s) 905
Psp6I CCWGG 1 cut(s) 1303
PspGI CCWGG 1 cut(s) 1303
PspN4I GGNNCC 4 cut(s) 484, 732, 995, 1634
PspPI GGNCC 1 cut(s) 2427
PspXI VCTCGAGB 1 cut(s) 1120
PstNI CAGNNNCTG 4 cut(s) 560, 758, 2063, 2753
PsuI RGATCY 2 cut(s) 1258, 1954
PvuI CGATCG 2 cut(s) 2322, 3055
PvuII CAGCTG 5 cut(s) 347, 853, 2063, 2372, 2807
RruI TCGCGA 2 cut(s) 261, 1708
RsaI GTAC 7 cut(s) 634, 741, 995, 1096, 2400, 3073, 3135
RsaNI GTAC 7 cut(s) 633, 740, 994, 1095, 2399, 3072, 3134
RseI CAYNNNNRTG 4 cut(s) 65, 804, 1059, 2211
SacI GAGCTC 1 cut(s) 905
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 8 cut(s) 77, 324, 426, 918, 1005, 1283, 2547, 3102
Sau96I GGNCC 1 cut(s) 2427
SchI GAGTC 7 cut(s) 212, 272, 566, 1556, 2027, 2035, 2759
ScrFI CCNGG 2 cut(s) 1305, 2605
SduI GDGCHC 3 cut(s) 905, 2877, 3116
SfaNI GCATC 7 cut(s) 1124, 1453, 2304, 2458, 2463, 2559, 2642
SfcI CTRYAG 1 cut(s) 2784
Sfr274I CTCGAG 2 cut(s) 1120, 2854
SinI GGWCC 1 cut(s) 2427
SlaI CTCGAG 2 cut(s) 1120, 2854
SmiMI CAYNNNNRTG 4 cut(s) 65, 804, 1059, 2211
SmlI CTYRAG 5 cut(s) 1065, 1120, 2080, 2854, 2876
SmoI CTYRAG 5 cut(s) 1065, 1120, 2080, 2854, 2876
SnaBI TACGTA 1 cut(s) 3075
SphI GCATGC 1 cut(s) 1773
SsiI CCGC 3 cut(s) 681, 1620, 1773
SspI AATATT 2 cut(s) 1087, 1220
SspMI CTAG 4 cut(s) 678, 698, 1674, 2819
SstI GAGCTC 1 cut(s) 905
StyD4I CCNGG 2 cut(s) 1303, 2603
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 9 cut(s) 637, 664, 744, 890, 1410, 1478, 1930, 2148, 2788
TaiI ACGT 2 cut(s) 634, 3077
TatI WGTACW 3 cut(s) 739, 2398, 3133
TauI GCSGC 1 cut(s) 1622
TfiI GAWTC 9 cut(s) 545, 701, 986, 1172, 1213, 1648, 2348, 2732, 2918
Tru1I TTAA 8 cut(s) 77, 324, 426, 918, 1005, 1283, 2547, 3102
Tru9I TTAA 8 cut(s) 77, 324, 426, 918, 1005, 1283, 2547, 3102
TscAI CASTG 7 cut(s) 669, 786, 1750, 1933, 2500, 2793, 2877
TseFI GTSAC 3 cut(s) 220, 627, 2558
Tsp45I GTSAC 3 cut(s) 220, 627, 2558
TspGWI ACGGA 2 cut(s) 2528, 3020
TspRI CASTG 7 cut(s) 669, 786, 1750, 1933, 2500, 2793, 2877
Van91I CCANNNNNTGG 2 cut(s) 503, 1892
VpaK11BI GGWCC 1 cut(s) 2427
XagI CCTNNNNNAGG 1 cut(s) 1187
XapI RAATTY 5 cut(s) 194, 667, 1017, 1810, 2377
XceI RCATGY 2 cut(s) 1364, 1773
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 2 cut(s) 1120, 2854
XmiI GTMKAC 1 cut(s) 2888
XmnI GAANNNNTTC 2 cut(s) 549, 2159
XspI CTAG 4 cut(s) 678, 698, 1674, 2819
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.