FvH4_4g24150

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005915
Physical Location & Seq
Reverse (-)
26423785 .. 26424892
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24150.t3

Sequence Viewer

Length: 522 bp
ATGCGAGGTCGAAAACGCAGCTGTCCTGGTGTTGCACCAGCCAGAGAAGCTCGTAGAGTCCGCAGGGGCGAAAAGGAAATCGACTATGAGATGATCCACCATATGGAAGCCGAGGCTTACCATGCCGTGTTGAAGGCCTTTAGTGCACAATCAGATAGTCTCTCTTGGGATCGGGTGGCCATGATGACAAAGCTGCGGAAGGAACTTAACATTACAGATGATGAACATGCAGAGCTGCTTGTGCAGATTAGGCGATCCGATGAGTCTGTCAGAGCGTTGAGGGAATGGCGAAATTCGAATGGTACTGCCATTCAAGAGTTTGGTAATGTCGATAAAAGATCCGGTTTTATTCAGATTCGTGCTACTAACGAAATCATTCATGAGGTTGAGAAGCTGCTTGACTGTCAAGAAATCCTCTCACCGGCGCAATTGGAAGCGGCAAAGTTGGTCCTGCAAGAGCACGAAAGGGATATCCTGGAAGTACTTGATCAACTGGCTATTGATGAAGCTGCTGGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

174

Amino Acids

19.8

Weight (kDa)

5.58

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 30 - 99 7.7e-25 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 3 cut(s) 61, 196, 437
AclWI GGATC 4 cut(s) 88, 177, 249, 333
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 292
AfaI GTAC 2 cut(s) 304, 483
AfiI CCNNNNNNNGG 2 cut(s) 103, 421
AgsI TTSAA 2 cut(s) 133, 314
AjnI CCWGG 2 cut(s) 25, 474
AluBI AGCT 6 cut(s) 21, 50, 193, 235, 394, 509
AluI AGCT 6 cut(s) 21, 50, 193, 235, 394, 509
Alw21I GWGCWC 2 cut(s) 148, 462
Alw26I GTCTC 1 cut(s) 164
Alw44I GTGCAC 1 cut(s) 144
AlwI GGATC 4 cut(s) 88, 177, 249, 333
AoxI GGCC 2 cut(s) 135, 177
ApaLI GTGCAC 1 cut(s) 144
ApeKI GCWGC 5 cut(s) 18, 193, 235, 394, 509
ApoI RAATTY 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 1 cut(s) 411
AsuII TTCGAA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 448
BaeGI GKGCMC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 148, 462
BbvI GCAGC 5 cut(s) 30, 180, 222, 381, 496
BceAI ACGGC 1 cut(s) 110
BciT130I CCWGG 2 cut(s) 27, 476
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 1 cut(s) 164
BisI GCNGC 6 cut(s) 19, 194, 236, 395, 438, 510
BlsI GCNGC 6 cut(s) 20, 195, 237, 396, 439, 511
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 2 cut(s) 27, 476
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 448
BmrFI CCNGG 2 cut(s) 27, 476
Bpu14I TTCGAA 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 1 cut(s) 341
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 421
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 498
BseBI CCWGG 2 cut(s) 27, 476
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 103, 421
BseNI ACTGG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 5 cut(s) 30, 180, 222, 381, 496
BsgI GTGCAG 1 cut(s) 263
BshFI GGCC 2 cut(s) 137, 179
BsiHKAI GWGCWC 2 cut(s) 148, 462
BsiSI CCGG 2 cut(s) 342, 422
BslI CCNNNNNNNGG 2 cut(s) 103, 421
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 2 cut(s) 137, 179
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 148, 462
Bsp143I GATC 5 cut(s) 93, 169, 254, 338, 487
BspACI CCGC 3 cut(s) 61, 196, 437
BspANI GGCC 2 cut(s) 137, 179
BspHI TCATGA 1 cut(s) 379
BspPI GGATC 4 cut(s) 88, 177, 249, 333
BspT104I TTCGAA 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 1 cut(s) 498
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 5 cut(s) 93, 169, 254, 338, 487
Bst2UI CCWGG 2 cut(s) 27, 476
Bst4CI ACNGT 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 296
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 5 cut(s) 96, 172, 257, 341, 490
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 5 cut(s) 93, 169, 254, 338, 487
BstMWI GCNNNNNNNGC 6 cut(s) 47, 122, 143, 241, 250, 515
BstNI CCWGG 2 cut(s) 27, 476
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 25, 474
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 5 cut(s) 30, 180, 222, 381, 496
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BsuRI GGCC 2 cut(s) 137, 179
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 303, 482
CviAII CATG 4 cut(s) 122, 181, 227, 380
CviQI GTAC 2 cut(s) 303, 482
DpnI GATC 5 cut(s) 95, 171, 256, 340, 489
DpnII GATC 5 cut(s) 93, 169, 254, 338, 487
EaeI YGGCCR 1 cut(s) 177
Eco147I AGGCCT 1 cut(s) 137
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 448
EcoRII CCWGG 2 cut(s) 25, 474
EcoRV GATATC 1 cut(s) 472
FaeI CATG 4 cut(s) 125, 184, 230, 383
FaiI YATR 7 cut(s) 87, 102, 104, 123, 182, 228, 381
FatI CATG 4 cut(s) 121, 180, 226, 379
FauNDI CATATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 6 cut(s) 19, 194, 236, 395, 438, 510
Fsp4HI GCNGC 6 cut(s) 19, 194, 236, 395, 438, 510
GlaI GCGC 1 cut(s) 426
GluI GCNGC 6 cut(s) 19, 194, 236, 395, 438, 510
HaeIII GGCC 2 cut(s) 137, 179
HapII CCGG 2 cut(s) 342, 422
HhaI GCGC 1 cut(s) 427
Hin1II CATG 4 cut(s) 125, 184, 230, 383
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 3 cut(s) 57, 263, 355
HpaII CCGG 2 cut(s) 342, 422
HphI GGTGA 1 cut(s) 411
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 4 cut(s) 154, 259, 272, 354
Hpy188III TCNNGA 3 cut(s) 314, 380, 407
Hpy8I GTNNAC 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 127, 193
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 5 cut(s) 35, 146, 230, 244, 454
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 122, 143, 241, 250, 515
Hsp92II CATG 4 cut(s) 125, 184, 230, 383
HspAI GCGC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 5 cut(s) 93, 169, 254, 338, 487
Lsp1109I GCAGC 5 cut(s) 30, 180, 222, 381, 496
MalI GATC 5 cut(s) 95, 171, 256, 340, 489
MboI GATC 5 cut(s) 93, 169, 254, 338, 487
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 2 cut(s) 148, 462
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 2 cut(s) 292, 428
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 2 cut(s) 66, 272
MnlI CCTC 4 cut(s) 106, 273, 376, 425
Mox20I TGGCCA 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 375
MscI TGGCCA 1 cut(s) 179
MseI TTAA 2 cut(s) 207, 520
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 2 cut(s) 342, 422
MspR9I CCNGG 2 cut(s) 27, 476
MunI CAATTG 1 cut(s) 428
MvaI CCWGG 2 cut(s) 27, 476
MwoI GCNNNNNNNGC 6 cut(s) 47, 122, 143, 241, 250, 515
NdeI CATATG 1 cut(s) 102
NdeII GATC 5 cut(s) 93, 169, 254, 338, 487
NlaIII CATG 4 cut(s) 125, 184, 230, 383
NmeAIII GCCGAG 1 cut(s) 136
NspI RCATGY 1 cut(s) 230
NspV TTCGAA 1 cut(s) 296
PagI TCATGA 1 cut(s) 379
PceI AGGCCT 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 355
PflMI CCANNNNNTGG 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 6 cut(s) 20, 195, 237, 396, 439, 511
PleI GAGTC 2 cut(s) 65, 271
PpsI GAGTC 2 cut(s) 65, 271
Psp6I CCWGG 2 cut(s) 25, 474
PspGI CCWGG 2 cut(s) 25, 474
PspPI GGNCC 1 cut(s) 448
PsuI RGATCY 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 2 cut(s) 304, 483
RsaNI GTAC 2 cut(s) 303, 482
SaqAI TTAA 2 cut(s) 207, 520
SatI GCNGC 6 cut(s) 19, 194, 236, 395, 438, 510
Sau3AI GATC 5 cut(s) 93, 169, 254, 338, 487
Sau96I GGNCC 1 cut(s) 448
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 2 cut(s) 66, 272
ScrFI CCNGG 2 cut(s) 27, 476
SduI GDGCHC 2 cut(s) 148, 462
SetI ASST 8 cut(s) 10, 23, 52, 195, 237, 387, 396, 511
SfuI TTCGAA 1 cut(s) 296
SgrAI CRCCGGYG 1 cut(s) 421
SinI GGWCC 1 cut(s) 448
Sse9I AATT 2 cut(s) 292, 428
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 3 cut(s) 61, 196, 437
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 25, 474
TaaI ACNGT 1 cut(s) 404
TaqI TCGA 4 cut(s) 10, 81, 296, 330
TasI AATT 2 cut(s) 292, 428
TatI WGTACW 1 cut(s) 481
TauI GCSGC 1 cut(s) 440
TfiI GAWTC 1 cut(s) 355
Tru1I TTAA 2 cut(s) 207, 520
Tru9I TTAA 2 cut(s) 207, 520
TseI GCWGC 5 cut(s) 18, 193, 235, 394, 509
TspDTI ATGAA 3 cut(s) 237, 368, 519
Van91I CCANNNNNTGG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 230
XmnI GAANNNNTTC 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.