Rh4AG302900

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005915
Physical Location & Seq
Reverse (-)
61554274 .. 61555310
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG302900.1

Sequence Viewer

Length: 525 bp
ATGCGAGGTCGCAAACGCAAGTGCTCTGGTGTTGCACCTTCAAGAGAAGCTCGTAGAGTCCGAAGGCCGGGTGAAAGGAAAATCGACTATGAGATGATCCACCATATGGAAGCCGAGGCTTACCATGCTGTCTTGAAGGCCTTTAGTGCTCAATCTGATAGTATCTCTTGGGATCGGGTGGCGCTGATGACTAAGCTGCGGAAGGAACTTAACATTACAGATGATGAACATGGAGAGCTACTCGTGAAGATTAGGCAATCCGACGAGTCTATCAGAGCGTTAAGGGAATGGCGAAATTCGAATGGTACTGCTGCTCAAGAGTTTGGTAATGTCGATAAAAGATCTGGTTTTATTCAGATTGGTGCTACGGATAAAATCATTCATGAGGTTGAGAAGCTGCTTGACAGTCAAGATATCCTCTCCCCTGCGCAATTGGAACGGGCAAAGTTGGTCCTTCGAGAGCACGAAAGGGCTATCCTTGAAGCACTTGATCAGCTGGCTATAGATGAAGCTGCTGGGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

174

Amino Acids

19.84

Weight (kDa)

6.61

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 31 - 100 7.9e-25 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 429
AccB7I CCANNNNNTGG 1 cut(s) 106
AciI CCGC 1 cut(s) 199
AclWI GGATC 2 cut(s) 91, 180
AcsI RAATTY 1 cut(s) 295
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 67, 106
AgsI TTSAA 3 cut(s) 42, 136, 482
AluBI AGCT 6 cut(s) 50, 196, 238, 397, 496, 512
AluI AGCT 6 cut(s) 50, 196, 238, 397, 496, 512
Alw21I GWGCWC 3 cut(s) 26, 151, 465
AlwI GGATC 2 cut(s) 91, 180
AoxI GGCC 2 cut(s) 65, 138
ApeKI GCWGC 4 cut(s) 196, 311, 397, 512
ApoI RAATTY 1 cut(s) 295
AspLEI GCGC 2 cut(s) 184, 430
AspS9I GGNCC 1 cut(s) 451
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 83
AsuII TTCGAA 1 cut(s) 299
AvaII GGWCC 1 cut(s) 451
BauI CACGAG 1 cut(s) 242
Bbv12I GWGCWC 3 cut(s) 26, 151, 465
BbvI GCAGC 4 cut(s) 183, 298, 384, 499
BclI TGATCA 1 cut(s) 490
BcnI CCSGG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 185
BglII AGATCT 1 cut(s) 341
BisI GCNGC 4 cut(s) 197, 312, 398, 513
BlsI GCNGC 4 cut(s) 198, 313, 399, 514
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 451
BmrFI CCNGG 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 225, 257
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 114
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 106
BseDI CCNNGG 1 cut(s) 114
BseLI CCNNNNNNNGG 2 cut(s) 67, 106
BseXI GCAGC 4 cut(s) 183, 298, 384, 499
BseYI CCCAGC 1 cut(s) 515
BshFI GGCC 2 cut(s) 67, 140
BsiHKAI GWGCWC 3 cut(s) 26, 151, 465
BsiSI CCGG 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 67, 106
BsnI GGCC 2 cut(s) 67, 140
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 3 cut(s) 26, 151, 465
Bsp143I GATC 4 cut(s) 96, 172, 341, 490
BspACI CCGC 1 cut(s) 199
BspANI GGCC 2 cut(s) 67, 140
BspHI TCATGA 1 cut(s) 382
BspPI GGATC 2 cut(s) 91, 180
BspT104I TTCGAA 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 4 cut(s) 96, 172, 341, 490
BssSI CACGAG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 242
Bst4CI ACNGT 1 cut(s) 407
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 2 cut(s) 184, 430
BstKTI GATC 4 cut(s) 99, 175, 344, 493
BstMBI GATC 4 cut(s) 96, 172, 341, 490
BstMWI GCNNNNNNNGC 2 cut(s) 125, 146
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 183, 298, 384, 499
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
BsuRI GGCC 2 cut(s) 67, 140
Cac8I GCNNGC 1 cut(s) 498
CciI TCATGA 1 cut(s) 382
CfoI GCGC 2 cut(s) 184, 430
Cfr13I GGNCC 1 cut(s) 451
Csp6I GTAC 1 cut(s) 306
CviAII CATG 3 cut(s) 125, 230, 383
CviQI GTAC 1 cut(s) 306
DdeI CTNAG 1 cut(s) 192
DpnI GATC 4 cut(s) 98, 174, 343, 492
DpnII GATC 4 cut(s) 96, 172, 341, 490
Eco147I AGGCCT 1 cut(s) 140
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 451
EcoRV GATATC 1 cut(s) 415
FaeI CATG 3 cut(s) 128, 233, 386
FaiI YATR 7 cut(s) 90, 105, 107, 126, 231, 384, 503
FatI CATG 3 cut(s) 124, 229, 382
FauNDI CATATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 4 cut(s) 197, 312, 398, 513
Fsp4HI GCNGC 4 cut(s) 197, 312, 398, 513
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 2 cut(s) 183, 429
GluI GCNGC 4 cut(s) 197, 312, 398, 513
GsaI CCCAGC 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 2 cut(s) 67, 140
HapII CCGG 1 cut(s) 68
HhaI GCGC 2 cut(s) 184, 430
Hin1II CATG 3 cut(s) 128, 233, 386
Hin6I GCGC 2 cut(s) 182, 428
HinP1I GCGC 2 cut(s) 182, 428
HinfI GANTC 2 cut(s) 57, 266
HpaII CCGG 1 cut(s) 68
HphI GGTGA 1 cut(s) 83
Hpy188I TCNGA 5 cut(s) 62, 157, 262, 275, 357
Hpy188III TCNNGA 7 cut(s) 42, 133, 244, 317, 383, 410, 458
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 5 cut(s) 48, 57, 130, 196, 464
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4V TGCA 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 146
HpyF3I CTNAG 1 cut(s) 192
Hsp92II CATG 3 cut(s) 128, 233, 386
HspAI GCGC 2 cut(s) 182, 428
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 4 cut(s) 96, 172, 341, 490
LpnPI CCDG 6 cut(s) 12, 81, 330, 438, 482, 501
Lsp1109I GCAGC 4 cut(s) 183, 298, 384, 499
MalI GATC 4 cut(s) 98, 174, 343, 492
MboI GATC 4 cut(s) 96, 172, 341, 490
MboII GAAGA 1 cut(s) 259
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 3 cut(s) 26, 151, 465
MluCI AATT 2 cut(s) 295, 431
MlyI GAGTC 2 cut(s) 66, 275
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 3 cut(s) 109, 379, 428
MseI TTAA 2 cut(s) 210, 281
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 69
MunI CAATTG 1 cut(s) 431
MwoI GCNNNNNNNGC 2 cut(s) 125, 146
NciI CCSGG 1 cut(s) 69
NdeI CATATG 1 cut(s) 105
NdeII GATC 4 cut(s) 96, 172, 341, 490
NlaIII CATG 3 cut(s) 128, 233, 386
NmeAIII GCCGAG 1 cut(s) 139
NsbI TGCGCA 1 cut(s) 429
NspV TTCGAA 1 cut(s) 299
PagI TCATGA 1 cut(s) 382
PceI AGGCCT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PflMI CCANNNNNTGG 1 cut(s) 106
PkrI GCNGC 4 cut(s) 198, 313, 399, 514
PleI GAGTC 2 cut(s) 65, 274
PpsI GAGTC 2 cut(s) 65, 274
PspFI CCCAGC 1 cut(s) 515
PspPI GGNCC 1 cut(s) 451
PsuI RGATCY 1 cut(s) 341
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
SaqAI TTAA 2 cut(s) 210, 281
SatI GCNGC 4 cut(s) 197, 312, 398, 513
Sau3AI GATC 4 cut(s) 96, 172, 341, 490
Sau96I GGNCC 1 cut(s) 451
SchI GAGTC 2 cut(s) 66, 275
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 3 cut(s) 26, 151, 465
SetI ASST 9 cut(s) 10, 40, 52, 198, 240, 390, 399, 498, 514
SfcI CTRYAG 1 cut(s) 501
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 2 cut(s) 295, 431
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 1 cut(s) 199
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 407
TaqI TCGA 4 cut(s) 84, 299, 333, 457
TasI AATT 2 cut(s) 295, 431
Tru1I TTAA 2 cut(s) 210, 281
Tru9I TTAA 2 cut(s) 210, 281
TseI GCWGC 4 cut(s) 196, 311, 397, 512
TspDTI ATGAA 3 cut(s) 240, 371, 522
TspGWI ACGGA 1 cut(s) 383
Van91I CCANNNNNTGG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 451
XapI RAATTY 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.