pycom14g00640

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005915
Physical Location & Seq
Forward (+)
426681 .. 430020
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00640.1

Sequence Viewer

Length: 996 bp
ATGTCTCCAGCAACAAAACCTCATGGAAAGGATTTATGGGATATGGAGCTTCACATTCACCGTTTGGAAACAGAGGCATACCATGCTGTTCTGAAGGCTTTTAGCGCTCAATCTAATAACCTAACTTGGGCACGGGAGGCACTTATGACTGAGCTGCGGAGGGAACTTAATATTACAGATGATGAACATGGACAGCTGCTCATTAAGATTAAGTCAGATCAGACAATCAAAATGATAAGGGAATGGCGGAATGGTGCCCCTTGTGCTCAAGAGTATAGTGAGGATGCTCCTGGATATCCTCCTCCTCAAGTAGTGATCAATGCTGAACCGAGAAAACTGGAGACTTCACATCCTCCTGTTTCAAAGTCTCAGAAATGTGTCTCATACAGTCATTCTTGTAAAGAAACTGCCCCTTCTGCTGCACCAGATGCCCACACCAAAAACACTAATGGAATGAGAGTCAAACGTGGAAAAAACTTAACTCAAGGAAACAGCCTTGTCGTGAGCTTGAAGTACCAAGGACAACTTAGCAATGATAAAAGTACCGACCCAGCAATGCTCTCCTCTGAGAAAGGCGGGCAGGCTATGAATATAAGTTGTCATGACCCTCAGCCTCAGGTTCCATCTGGTAGCAGAAGTACTGGACTGGTAAAACCCCAATCGAAGATAGGTTCCCGTGCACCTGACTCTGATAATTTCAAGGAAAGTTGTGATTTTATTAGGATTCGTGCTACAGATCAAGTTATTCATCAGGCTTATAAGCTGCTTAGTCAAGAAAGAGTAGTCCCACTTCATATAGAAAAGGCAAAGCTAATCCTTCGAGAGCATGAAAGAGCTATCCTCAACGCACTTGATAAACTGCCTGATGTGTCAGAGATGGCCGATTCTCCCAAGCAAATGCAGCACAAGTATCCTCATGAGGAGCTTCCAGGAATGGGACGTGAAATGTTGATACGCAATGACTTGTATGGACAGGCTGGTAGGCTGGCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

332

Amino Acids

37.08

Weight (kDa)

8.46

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 18 - 85 4.6e-26 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AccB1I GGYRCC 1 cut(s) 254
AciI CCGC 3 cut(s) 157, 247, 576
AcoI YGGCCR 1 cut(s) 879
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 3 cut(s) 515, 544, 640
AfeI AGCGCT 1 cut(s) 106
AfiI CCNNNNNNNGG 2 cut(s) 127, 935
AgsI TTSAA 3 cut(s) 363, 511, 700
AjiI CACGTC 1 cut(s) 941
AjnI CCWGG 2 cut(s) 289, 928
AluBI AGCT 8 cut(s) 49, 154, 196, 507, 763, 811, 836, 925
AluI AGCT 8 cut(s) 49, 154, 196, 507, 763, 811, 836, 925
Alw21I GWGCWC 2 cut(s) 268, 682
Alw26I GTCTC 4 cut(s) 9, 335, 372, 385
Alw44I GTGCAC 1 cut(s) 678
Aor51HI AGCGCT 1 cut(s) 106
AoxI GGCC 1 cut(s) 879
ApaLI GTGCAC 1 cut(s) 678
ApeKI GCWGC 5 cut(s) 154, 196, 419, 763, 901
AspLEI GCGC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 50
AxyI CCTNAGG 1 cut(s) 615
BaeGI GKGCMC 3 cut(s) 133, 259, 682
BanI GGYRCC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 774, 806
Bbv12I GWGCWC 2 cut(s) 268, 682
BbvCI CCTCAGC 1 cut(s) 609
BbvI GCAGC 5 cut(s) 141, 183, 406, 750, 913
BccI CCATC 2 cut(s) 631, 871
BciT130I CCWGG 2 cut(s) 291, 930
BciVI GTATCC 1 cut(s) 921
BclI TGATCA 1 cut(s) 315
BcoDI GTCTC 4 cut(s) 9, 335, 372, 385
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 1 cut(s) 108
BfuI GTATCC 1 cut(s) 921
BisI GCNGC 5 cut(s) 155, 197, 420, 764, 902
BlsI GCNGC 5 cut(s) 156, 198, 421, 765, 903
BmcAI AGTACT 1 cut(s) 640
Bme1390I CCNGG 2 cut(s) 291, 930
BmgBI CACGTC 1 cut(s) 941
BmiI GGNNCC 3 cut(s) 256, 621, 673
BmrFI CCNGG 2 cut(s) 291, 930
BmsI GCATC 2 cut(s) 274, 418
BplI GAGNNNNNCTC 2 cut(s) 825, 857
BpmI CTGGAG 1 cut(s) 359
Bpu10I CCTNAGC 1 cut(s) 609
BpuEI CTTGAG 3 cut(s) 252, 291, 468
BsaJI CCNNGG 1 cut(s) 517
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 935
Bse1I ACTGG 3 cut(s) 342, 646, 651
Bse21I CCTNAGG 1 cut(s) 615
Bse3DI GCAATG 3 cut(s) 538, 561, 964
BseBI CCWGG 2 cut(s) 291, 930
BseDI CCNNGG 1 cut(s) 517
BseGI GGATG 2 cut(s) 289, 349
BseLI CCNNNNNNNGG 2 cut(s) 127, 935
BseMI GCAATG 3 cut(s) 538, 561, 964
BseMII CTCAG 5 cut(s) 141, 383, 558, 623, 629
BseNI ACTGG 3 cut(s) 342, 646, 651
BseRI GAGGAG 4 cut(s) 291, 294, 553, 935
BseSI GKGCMC 3 cut(s) 133, 259, 682
BseXI GCAGC 5 cut(s) 141, 183, 406, 750, 913
BseYI CCCAGC 1 cut(s) 550
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 1 cut(s) 881
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 2 cut(s) 268, 682
BslFI GGGAC 2 cut(s) 770, 951
BslI CCNNNNNNNGG 2 cut(s) 127, 935
BsmAI GTCTC 4 cut(s) 9, 335, 372, 385
BsmFI GGGAC 2 cut(s) 770, 951
BsnI GGCC 1 cut(s) 881
Bsp1286I GDGCHC 4 cut(s) 133, 259, 268, 682
Bsp143I GATC 3 cut(s) 217, 315, 736
BspACI CCGC 3 cut(s) 157, 247, 576
BspANI GGCC 1 cut(s) 881
BspCNI CTCAG 5 cut(s) 142, 382, 559, 622, 628
BspHI TCATGA 2 cut(s) 601, 916
BspLI GGNNCC 3 cut(s) 256, 621, 673
BspT107I GGYRCC 1 cut(s) 254
BsrDI GCAATG 3 cut(s) 538, 561, 964
BsrI ACTGG 3 cut(s) 342, 646, 651
BssECI CCNNGG 1 cut(s) 517
BssMI GATC 3 cut(s) 217, 315, 736
BssT1I CCWWGG 1 cut(s) 517
Bst2UI CCWGG 2 cut(s) 291, 930
Bst4CI ACNGT 2 cut(s) 62, 389
BstAPI GCANNNNNTGC 2 cut(s) 83, 428
BstC8I GCNNGC 3 cut(s) 578, 582, 987
BstDEI CTNAG 7 cut(s) 150, 369, 527, 567, 609, 615, 767
BstF5I GGATG 2 cut(s) 289, 349
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 3 cut(s) 220, 318, 739
BstMAI GTCTC 4 cut(s) 9, 335, 372, 385
BstMBI GATC 3 cut(s) 217, 315, 736
BstMWI GCNNNNNNNGC 7 cut(s) 83, 104, 137, 263, 416, 428, 901
BstNI CCWGG 2 cut(s) 291, 930
BstSCI CCNGG 2 cut(s) 289, 928
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 3 cut(s) 133, 259, 682
BstV1I GCAGC 5 cut(s) 141, 183, 406, 750, 913
Bsu36I CCTNAGG 1 cut(s) 615
BsuI GTATCC 1 cut(s) 921
BsuRI GGCC 1 cut(s) 881
BtrI CACGTC 1 cut(s) 941
BtsCI GGATG 2 cut(s) 289, 349
Cac8I GCNNGC 3 cut(s) 578, 582, 987
CciI TCATGA 2 cut(s) 601, 916
CfoI GCGC 1 cut(s) 107
Csp6I GTAC 3 cut(s) 514, 543, 639
CviAII CATG 6 cut(s) 23, 83, 188, 602, 827, 917
CviQI GTAC 3 cut(s) 514, 543, 639
DdeI CTNAG 7 cut(s) 150, 369, 527, 567, 609, 615, 767
DpnI GATC 3 cut(s) 219, 317, 738
DpnII GATC 3 cut(s) 217, 315, 736
EaeI YGGCCR 1 cut(s) 879
EciI GGCGGA 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 517
Eco32I GATATC 1 cut(s) 296
Eco47III AGCGCT 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 113
Eco81I CCTNAGG 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 289, 928
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 517
FaeI CATG 6 cut(s) 26, 86, 191, 605, 830, 920
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 2 cut(s) 770, 951
FatI CATG 6 cut(s) 22, 82, 187, 601, 826, 916
FauI CCCGC 1 cut(s) 569
FbaI TGATCA 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 155, 197, 420, 764, 902
FokI GGATG 2 cut(s) 296, 336
Fsp4HI GCNGC 5 cut(s) 155, 197, 420, 764, 902
GlaI GCGC 1 cut(s) 106
GluI GCNGC 5 cut(s) 155, 197, 420, 764, 902
GsaI CCCAGC 1 cut(s) 554
GsuI CTGGAG 1 cut(s) 359
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 1 cut(s) 881
HhaI GCGC 1 cut(s) 107
Hin1II CATG 6 cut(s) 26, 86, 191, 605, 830, 920
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 4 cut(s) 459, 686, 724, 884
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 680
Hpy188I TCNGA 7 cut(s) 93, 217, 222, 372, 568, 691, 874
Hpy188III TCNNGA 6 cut(s) 269, 502, 602, 773, 821, 917
Hpy8I GTNNAC 1 cut(s) 680
HpyAV CCTTC 3 cut(s) 88, 423, 827
HpyCH4III ACNGT 2 cut(s) 62, 389
HpyCH4IV ACGT 2 cut(s) 466, 940
HpyCH4V TGCA 3 cut(s) 422, 680, 901
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 104, 137, 263, 416, 428, 901
HpyF3I CTNAG 7 cut(s) 150, 369, 527, 567, 609, 615, 767
HpySE526I ACGT 2 cut(s) 466, 940
Hsp92II CATG 6 cut(s) 26, 86, 191, 605, 830, 920
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 3 cut(s) 217, 315, 736
LmnI GCTCC 3 cut(s) 46, 292, 922
Lsp1109I GCAGC 5 cut(s) 141, 183, 406, 750, 913
LweI GCATC 2 cut(s) 274, 418
MaeII ACGT 2 cut(s) 466, 940
MalI GATC 3 cut(s) 219, 317, 738
MboI GATC 3 cut(s) 217, 315, 736
MboII GAAGA 1 cut(s) 676
MhlI GDGCHC 4 cut(s) 133, 259, 268, 682
MluCI AATT 1 cut(s) 694
MlyI GAGTC 2 cut(s) 468, 680
MseI TTAA 4 cut(s) 168, 204, 210, 479
MspA1I CMGCKG 1 cut(s) 196
MspR9I CCNGG 2 cut(s) 291, 930
MvaI CCWGG 2 cut(s) 291, 930
MwoI GCNNNNNNNGC 7 cut(s) 83, 104, 137, 263, 416, 428, 901
NdeII GATC 3 cut(s) 217, 315, 736
NlaIII CATG 6 cut(s) 26, 86, 191, 605, 830, 920
NlaIV GGNNCC 3 cut(s) 256, 621, 673
PagI TCATGA 2 cut(s) 601, 916
PfeI GAWTC 2 cut(s) 724, 884
PfoI TCCNGGA 2 cut(s) 289, 928
PkrI GCNGC 5 cut(s) 156, 198, 421, 765, 903
PleI GAGTC 2 cut(s) 467, 680
PpsI GAGTC 2 cut(s) 467, 680
PsiI TTATAA 1 cut(s) 759
Psp6I CCWGG 2 cut(s) 289, 928
PspFI CCCAGC 1 cut(s) 550
PspGI CCWGG 2 cut(s) 289, 928
PspN4I GGNNCC 3 cut(s) 256, 621, 673
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 3 cut(s) 515, 544, 640
RsaNI GTAC 3 cut(s) 514, 543, 639
SaqAI TTAA 4 cut(s) 168, 204, 210, 479
SatI GCNGC 5 cut(s) 155, 197, 420, 764, 902
Sau3AI GATC 3 cut(s) 217, 315, 736
ScaI AGTACT 1 cut(s) 640
SchI GAGTC 2 cut(s) 468, 680
ScrFI CCNGG 2 cut(s) 291, 930
SduI GDGCHC 4 cut(s) 133, 259, 268, 682
SfaNI GCATC 2 cut(s) 274, 418
SfcI CTRYAG 1 cut(s) 732
SmlI CTYRAG 3 cut(s) 267, 306, 483
SmoI CTYRAG 3 cut(s) 267, 306, 483
Sse9I AATT 1 cut(s) 694
SsiI CCGC 3 cut(s) 157, 247, 576
SspI AATATT 1 cut(s) 172
StyD4I CCNGG 2 cut(s) 289, 928
StyI CCWWGG 1 cut(s) 517
TaaI ACNGT 2 cut(s) 62, 389
TaiI ACGT 2 cut(s) 469, 943
TaqI TCGA 2 cut(s) 662, 820
TasI AATT 1 cut(s) 694
TatI WGTACW 1 cut(s) 638
TfiI GAWTC 2 cut(s) 724, 884
Tru1I TTAA 4 cut(s) 168, 204, 210, 479
Tru9I TTAA 4 cut(s) 168, 204, 210, 479
TseI GCWGC 5 cut(s) 154, 196, 419, 763, 901
TspDTI ATGAA 5 cut(s) 198, 602, 737, 782, 843
VneI GTGCAC 1 cut(s) 678
ZrmI AGTACT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.