FvH4_6g03210

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005915
Physical Location & Seq
Forward (+)
1793731 .. 1793946
216 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03210.t1

Sequence Viewer

Length: 192 bp
ATGTGTGTGTTGCACCAGCCAGAGAAGCTCGTAGAGTCCGAAGGGGCGAAAGGTTACCATGCCGCGTTGAAGGCCTTTAGTGCACAATCTGATAGTCTCCCTTGGGATCGAGTGGCGCTGATGACTAAGCTGCGGAAGGAACTTAACATCACGGATGATGAACATGGAGAGCTGCTCGTGCAGATCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

64

Amino Acids

7.12

Weight (kDa)

5.83

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 17 - 62 1.4e-14 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 65
AciI CCGC 2 cut(s) 63, 133
AclWI GGATC 2 cut(s) 114, 178
AgsI TTSAA 1 cut(s) 70
AluBI AGCT 3 cut(s) 28, 130, 172
AluI AGCT 3 cut(s) 28, 130, 172
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 101
Alw44I GTGCAC 1 cut(s) 81
AlwI GGATC 2 cut(s) 114, 178
AoxI GGCC 1 cut(s) 72
ApaLI GTGCAC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 130, 172
AspLEI GCGC 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 85
BauI CACGAG 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 2 cut(s) 117, 159
BcoDI GTCTC 1 cut(s) 101
BfoI RGCGCY 1 cut(s) 119
BisI GCNGC 3 cut(s) 63, 131, 173
BlsI GCNGC 3 cut(s) 64, 132, 174
BplI GAGNNNNNCTC 2 cut(s) 159, 191
BsaJI CCNNGG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 160
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 2 cut(s) 117, 159
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 101
BsnI GGCC 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 2 cut(s) 106, 183
BspACI CCGC 2 cut(s) 63, 133
BspANI GGCC 1 cut(s) 74
BspFNI CGCG 1 cut(s) 65
BspPI GGATC 2 cut(s) 114, 178
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 2 cut(s) 106, 183
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 176
BstDEI CTNAG 1 cut(s) 126
BstEII GGTNACC 1 cut(s) 53
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 2 cut(s) 109, 186
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 106, 183
BstMWI GCNNNNNNNGC 4 cut(s) 25, 71, 80, 178
BstPI GGTNACC 1 cut(s) 53
BstSLI GKGCMC 1 cut(s) 85
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 117, 159
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 1 cut(s) 74
BtsCI GGATG 1 cut(s) 160
CfoI GCGC 1 cut(s) 118
CviAII CATG 2 cut(s) 59, 164
CviJI RGCY 5 cut(s) 19, 28, 74, 130, 172
CviKI_1 RGCY 5 cut(s) 19, 28, 74, 130, 172
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 108, 185
DpnII GATC 2 cut(s) 106, 183
Eco130I CCWWGG 1 cut(s) 101
Eco147I AGGCCT 1 cut(s) 74
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 2 cut(s) 62, 167
FaiI YATR 2 cut(s) 60, 165
FatI CATG 2 cut(s) 58, 163
Fnu4HI GCNGC 3 cut(s) 63, 131, 173
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 3 cut(s) 63, 131, 173
GlaI GCGC 1 cut(s) 117
GluI GCNGC 3 cut(s) 63, 131, 173
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 1 cut(s) 74
HhaI GCGC 1 cut(s) 118
Hin1II CATG 2 cut(s) 62, 167
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HinfI GANTC 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 3 cut(s) 40, 91, 188
Hpy8I GTNNAC 1 cut(s) 83
HpyAV CCTTC 3 cut(s) 35, 64, 130
HpyCH4V TGCA 3 cut(s) 13, 83, 181
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 71, 80, 178
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 2 cut(s) 62, 167
HspAI GCGC 1 cut(s) 116
Kzo9I GATC 2 cut(s) 106, 183
LpnPI CCDG 2 cut(s) 29, 33
Lsp1109I GCAGC 2 cut(s) 117, 159
MaeIII GTNAC 1 cut(s) 53
MalI GATC 2 cut(s) 108, 185
MboI GATC 2 cut(s) 106, 183
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 85
MlyI GAGTC 1 cut(s) 44
MseI TTAA 1 cut(s) 144
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 4 cut(s) 25, 71, 80, 178
NdeII GATC 2 cut(s) 106, 183
NlaIII CATG 2 cut(s) 62, 167
PceI AGGCCT 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PkrI GCNGC 3 cut(s) 64, 132, 174
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PspEI GGTNACC 1 cut(s) 53
PsuI RGATCY 1 cut(s) 183
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 63, 131, 173
Sau3AI GATC 2 cut(s) 106, 183
SchI GAGTC 1 cut(s) 44
SduI GDGCHC 1 cut(s) 85
SetI ASST 4 cut(s) 30, 55, 132, 174
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 2 cut(s) 63, 133
StuI AGGCCT 1 cut(s) 74
StyI CCWWGG 1 cut(s) 101
TaqI TCGA 1 cut(s) 109
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseI GCWGC 2 cut(s) 130, 172
TspDTI ATGAA 1 cut(s) 174
TspGWI ACGGA 1 cut(s) 167
VneI GTGCAC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.