Rw4G026330

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005915
Physical Location & Seq
Reverse (-)
52489480 .. 52490001
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026330.1

Sequence Viewer

Length: 522 bp
ATGCGAGGTCGCAAACGCAAGTGCTCTGGTGTTGCACCTTCAAGAGAAGCTCGTAGAGTCCGAAGGGGTGAAAGGAAAATCGACTATGAGATGATCCACCATATGGAAGCCGAGGCTTACCATGCTGTCTTGAAGGCCTTTAGTGCTCAATCTGATAGTATCTCTTGGGATCGGGTGGCGCTGATGACTAAGCTGCGGAAGGAACTTAACATTACAGATGATGAACATGGAGAGCTACTCGTGAAGATTAGGCAATCCGACGAGTCTATCGGGGCGTTAAGGGAATGGCGAAATTCGAATGCTACCGCCGCTCAAGAGTTTGGTAATGTCGCTAAAAGATCTGGTTTTATTCAGATCGGTGCTACGGATAAAATCATTCATGAGGTTGAGAAGCTGCTTGACAGTCAAGATATCCTCTCCCCTGCGCAATTGGAACGGGCAAACTTGGTCCTTCGACAGCACGAAAGGGCTATCCTTGAAGCACTTGATCAGCTGGCTATAGACGAAGCTGCTGGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

173

Amino Acids

19.6

Weight (kDa)

6.61

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 30 - 99 1.9e-24 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 426
AccB7I CCANNNNNTGG 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 311
AciI CCGC 3 cut(s) 196, 306, 309
AclWI GGATC 2 cut(s) 88, 177
AcsI RAATTY 1 cut(s) 292
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 3 cut(s) 42, 133, 479
AluBI AGCT 6 cut(s) 50, 193, 235, 394, 493, 509
AluI AGCT 6 cut(s) 50, 193, 235, 394, 493, 509
Alw21I GWGCWC 2 cut(s) 26, 148
AlwI GGATC 2 cut(s) 88, 177
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 3 cut(s) 193, 394, 509
ApoI RAATTY 1 cut(s) 292
AspLEI GCGC 2 cut(s) 181, 427
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 1 cut(s) 80
AsuII TTCGAA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 448
BauI CACGAG 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 26, 148
BbvI GCAGC 3 cut(s) 180, 381, 496
BclI TGATCA 1 cut(s) 487
BfmI CTRYAG 1 cut(s) 498
BfoI RGCGCY 1 cut(s) 182
BglII AGATCT 1 cut(s) 338
BisI GCNGC 4 cut(s) 194, 309, 395, 510
BlsI GCNGC 4 cut(s) 195, 310, 396, 511
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 448
BplI GAGNNNNNCTC 2 cut(s) 222, 254
Bpu14I TTCGAA 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 103
BseXI GCAGC 3 cut(s) 180, 381, 496
BshFI GGCC 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 26, 148
BslI CCNNNNNNNGG 1 cut(s) 103
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 26, 148
Bsp143I GATC 5 cut(s) 93, 169, 338, 354, 487
BspACI CCGC 3 cut(s) 196, 306, 309
BspANI GGCC 1 cut(s) 137
BspHI TCATGA 1 cut(s) 379
BspPI GGATC 2 cut(s) 88, 177
BspT104I TTCGAA 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 5 cut(s) 93, 169, 338, 354, 487
BssSI CACGAG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 2 cut(s) 181, 427
BstKTI GATC 5 cut(s) 96, 172, 341, 357, 490
BstMBI GATC 5 cut(s) 93, 169, 338, 354, 487
BstMWI GCNNNNNNNGC 3 cut(s) 122, 143, 308
BstSFI CTRYAG 1 cut(s) 498
BstV1I GCAGC 3 cut(s) 180, 381, 496
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BsuRI GGCC 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 495
CciI TCATGA 1 cut(s) 379
CfoI GCGC 2 cut(s) 181, 427
Cfr13I GGNCC 1 cut(s) 448
CviAII CATG 3 cut(s) 122, 227, 380
DdeI CTNAG 1 cut(s) 189
DpnI GATC 5 cut(s) 95, 171, 340, 356, 489
DpnII GATC 5 cut(s) 93, 169, 338, 354, 487
Eco147I AGGCCT 1 cut(s) 137
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 448
EcoRV GATATC 1 cut(s) 412
FaeI CATG 3 cut(s) 125, 230, 383
FaiI YATR 7 cut(s) 87, 102, 104, 123, 228, 381, 500
FatI CATG 3 cut(s) 121, 226, 379
FauNDI CATATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 4 cut(s) 194, 309, 395, 510
Fsp4HI GCNGC 4 cut(s) 194, 309, 395, 510
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 2 cut(s) 180, 426
GluI GCNGC 4 cut(s) 194, 309, 395, 510
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 1 cut(s) 137
HhaI GCGC 2 cut(s) 181, 427
Hin1II CATG 3 cut(s) 125, 230, 383
Hin6I GCGC 2 cut(s) 179, 425
HinP1I GCGC 2 cut(s) 179, 425
HinfI GANTC 2 cut(s) 57, 263
HphI GGTGA 1 cut(s) 80
Hpy188I TCNGA 4 cut(s) 62, 154, 259, 354
Hpy188III TCNNGA 6 cut(s) 42, 130, 241, 314, 380, 407
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 5 cut(s) 48, 57, 127, 193, 461
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 143, 308
HpyF3I CTNAG 1 cut(s) 189
Hsp92II CATG 3 cut(s) 125, 230, 383
HspAI GCGC 2 cut(s) 179, 425
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 5 cut(s) 93, 169, 338, 354, 487
LpnPI CCDG 5 cut(s) 12, 327, 435, 479, 498
Lsp1109I GCAGC 3 cut(s) 180, 381, 496
MaeIII GTNAC 1 cut(s) 515
MalI GATC 5 cut(s) 95, 171, 340, 356, 489
MbiI CCGCTC 1 cut(s) 311
MboI GATC 5 cut(s) 93, 169, 338, 354, 487
MboII GAAGA 1 cut(s) 256
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 2 cut(s) 26, 148
MluCI AATT 2 cut(s) 292, 428
MlyI GAGTC 2 cut(s) 66, 272
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 3 cut(s) 106, 376, 425
MseI TTAA 2 cut(s) 207, 278
MspA1I CMGCKG 1 cut(s) 493
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 304
MwoI GCNNNNNNNGC 3 cut(s) 122, 143, 308
NdeI CATATG 1 cut(s) 102
NdeII GATC 5 cut(s) 93, 169, 338, 354, 487
NlaIII CATG 3 cut(s) 125, 230, 383
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 515
NsbI TGCGCA 1 cut(s) 426
NspV TTCGAA 1 cut(s) 296
PagI TCATGA 1 cut(s) 379
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PctI GAATGC 1 cut(s) 304
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 4 cut(s) 195, 310, 396, 511
PleI GAGTC 2 cut(s) 65, 271
PpsI GAGTC 2 cut(s) 65, 271
PspPI GGNCC 1 cut(s) 448
PsuI RGATCY 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 493
SaqAI TTAA 2 cut(s) 207, 278
SatI GCNGC 4 cut(s) 194, 309, 395, 510
Sau3AI GATC 5 cut(s) 93, 169, 338, 354, 487
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 2 cut(s) 66, 272
SduI GDGCHC 2 cut(s) 26, 148
SetI ASST 9 cut(s) 10, 40, 52, 195, 237, 387, 396, 495, 511
SfcI CTRYAG 1 cut(s) 498
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
Sse9I AATT 2 cut(s) 292, 428
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 3 cut(s) 196, 306, 309
StuI AGGCCT 1 cut(s) 137
TaaI ACNGT 1 cut(s) 404
TaqI TCGA 3 cut(s) 81, 296, 454
TasI AATT 2 cut(s) 292, 428
TauI GCSGC 1 cut(s) 311
Tru1I TTAA 2 cut(s) 207, 278
Tru9I TTAA 2 cut(s) 207, 278
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 3 cut(s) 193, 394, 509
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 2 cut(s) 237, 368
TspGWI ACGGA 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.