Prupe.7G014800_v2.0.a1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0005915
Physical Location & Seq
Forward (+)
2191700 .. 2197881
6182 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G014800.2

Sequence Viewer

Length: 1104 bp
ATGGGCCTATATGGGATTTCTAACCAAATTCGGTTTCATGGAGGAGTTCAATTTTTGTCAAATATGGATCAGTTTTGGCTTTTTGGAAATTACAAACACAATCATGGTATTGGATCTGAAAGGATGTCTCCAGCAACAAAGCCTCATGGAAGGGATGATTGGGATATAGAGTTTCAGATTCACCGTATGGAAGCAAAGGCATACCATGCTGTGTTGAGGGCTTTTAGTGCTCAATCGAATAATCTAACTTGGGGACGAGAGGCACTTATGACTGAGCTGCGGAAGGAACTTAATATTTCAGATGATGAACATGGAGTTCTGCTCACGAAGATTAAGTCAGACGAGGCGATAAAAGTGATAAGGGAATGGCGGAATGATGCCCATTGTGCTCAAGAGTATGGTAAGGATGCTCCTAGATATCCTTCTAAACTAGTGAGCAATGCTCAACAGAGAGAAGTGGAGACTCAACATCCTCCTGTTTCAAAGTCTCAGAAATATTCCTCATGCAGTCAGCCTTGTGCAGAAGTTATCCCTTCTGCTACTGCTGCACCAGATGCCCAAATCAAAGACATTGGTAGAATGAGAGTGAAACTTGGAAGAAACTTTGTGACTCAAGGAAACAGCCCTTTTTTGAACTTGAAGTACCAAGGAGAACTTAGCAAGGCTCAAGGGAGTGGTGACTTGGCAATGCTCTCATCTGGGAATGGTAGACAGTCTATGCATATAATTGGTCGTAACCCTCAGGCACCATATGGTAGCAGAAGTACTGGACTGGTAAAATCTCAATTGAAGATGGGCTTTCACCCAATTGGCTCTGACAATTTAAACAAAAGTTCTGATTTGATTAAGATACGTGCTACAGACAAAGTCCTTTATGAGGTTGAGAAGCTATTGGATTGTCAAGAAAGCCTAGTCCCACTTCATGTTGAGAAGGCAAAGTTAATCCTTAAAGAGCATGAAAGAGCTATCCTTGAAGCACTTGACAAACTGCCTGATGTGTCAGACGTGGATGATTCTCCCAAGCAAATCTGGCATAAGTATCCTCATGAACAGCTTCCAGTAACGGGACGCAAAATGTTGATACGCAATGACTTCTATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

368

Amino Acids

41.41

Weight (kDa)

8.82

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 745
AccI GTMKAC 1 cut(s) 709
AciI CCGC 2 cut(s) 280, 370
AclWI GGATC 2 cut(s) 75, 121
AcsI RAATTY 1 cut(s) 27
AfaI GTAC 2 cut(s) 644, 766
AfiI CCNNNNNNNGG 2 cut(s) 877, 1064
AgsI TTSAA 6 cut(s) 50, 483, 634, 640, 790, 974
AhlI ACTAGT 1 cut(s) 430
AjiI CACGTC 1 cut(s) 1006
AjuI GAANNNNNNNTTGG 4 cut(s) 18, 50, 142, 174
AluBI AGCT 4 cut(s) 277, 889, 965, 1054
AluI AGCT 4 cut(s) 277, 889, 965, 1054
Alw21I GWGCWC 2 cut(s) 232, 391
Alw26I GTCTC 3 cut(s) 132, 455, 492
AlwI GGATC 2 cut(s) 75, 121
AoxI GGCC 1 cut(s) 4
ApeKI GCWGC 2 cut(s) 277, 545
ApoI RAATTY 1 cut(s) 27
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 1 cut(s) 1053
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 3 cut(s) 173, 689, 794
AxyI CCTNAGG 1 cut(s) 741
BanI GGYRCC 1 cut(s) 745
Bbv12I GWGCWC 2 cut(s) 232, 391
BbvI GCAGC 2 cut(s) 264, 532
BccI CCATC 1 cut(s) 787
BciVI GTATCC 1 cut(s) 1050
BcoDI GTCTC 3 cut(s) 132, 455, 492
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 3 cut(s) 414, 431, 911
BfmI CTRYAG 1 cut(s) 858
BfuI GTATCC 1 cut(s) 1050
BisI GCNGC 2 cut(s) 278, 546
BlsI GCNGC 2 cut(s) 279, 547
BmcAI AGTACT 1 cut(s) 766
BmgBI CACGTC 1 cut(s) 1006
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 747
BmsI GCATC 3 cut(s) 367, 397, 544
BplI GAGNNNNNCTC 4 cut(s) 306, 338, 427, 459
BpmI CTGGAG 1 cut(s) 114
BpuEI CTTGAG 3 cut(s) 375, 597, 651
BsaAI YACGTR 1 cut(s) 854
BsaJI CCNNGG 1 cut(s) 646
Bsc4I CCNNNNNNNGG 2 cut(s) 877, 1064
Bse1I ACTGG 3 cut(s) 772, 777, 1058
Bse21I CCTNAGG 1 cut(s) 741
Bse3DI GCAATG 3 cut(s) 445, 693, 1093
BseDI CCNNGG 1 cut(s) 646
BseGI GGATG 5 cut(s) 129, 160, 412, 469, 1015
BseLI CCNNNNNNNGG 2 cut(s) 877, 1064
BseMI GCAATG 3 cut(s) 445, 693, 1093
BseMII CTCAG 3 cut(s) 264, 503, 755
BseNI ACTGG 3 cut(s) 772, 777, 1058
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 264, 532
BsgI GTGCAG 2 cut(s) 531, 540
BshFI GGCC 1 cut(s) 6
BshNI GGYRCC 1 cut(s) 745
BsiHKAI GWGCWC 2 cut(s) 232, 391
BslFI GGGAC 3 cut(s) 267, 899, 1080
BslI CCNNNNNNNGG 2 cut(s) 877, 1064
BsmAI GTCTC 3 cut(s) 132, 455, 492
BsmFI GGGAC 3 cut(s) 267, 899, 1080
BsnI GGCC 1 cut(s) 6
Bsp1286I GDGCHC 2 cut(s) 232, 391
Bsp143I GATC 2 cut(s) 67, 113
BspACI CCGC 2 cut(s) 280, 370
BspANI GGCC 1 cut(s) 6
BspCNI CTCAG 3 cut(s) 265, 502, 754
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 1 cut(s) 747
BspPI GGATC 2 cut(s) 75, 121
BspT107I GGYRCC 1 cut(s) 745
BsrDI GCAATG 3 cut(s) 445, 693, 1093
BsrI ACTGG 3 cut(s) 772, 777, 1058
BssECI CCNNGG 1 cut(s) 646
BssMI GATC 2 cut(s) 67, 113
BssT1I CCWWGG 1 cut(s) 646
Bst4CI ACNGT 2 cut(s) 185, 714
BstAPI GCANNNNNTGC 2 cut(s) 206, 554
BstBAI YACGTR 1 cut(s) 854
BstDEI CTNAG 4 cut(s) 273, 489, 656, 741
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 5 cut(s) 129, 160, 412, 469, 1015
BstKTI GATC 2 cut(s) 70, 116
BstMAI GTCTC 3 cut(s) 132, 455, 492
BstMBI GATC 2 cut(s) 67, 113
BstMWI GCNNNNNNNGC 6 cut(s) 206, 227, 386, 545, 554, 1030
BstSFI CTRYAG 1 cut(s) 858
BstV1I GCAGC 2 cut(s) 264, 532
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
Bsu36I CCTNAGG 1 cut(s) 741
BsuI GTATCC 1 cut(s) 1050
BsuRI GGCC 1 cut(s) 6
BtrI CACGTC 1 cut(s) 1006
BtsCI GGATG 5 cut(s) 129, 160, 412, 469, 1015
CciI TCATGA 1 cut(s) 1045
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 1077
Csp6I GTAC 2 cut(s) 643, 765
CviAII CATG 9 cut(s) 38, 104, 146, 206, 311, 504, 923, 956, 1046
CviQI GTAC 2 cut(s) 643, 765
DdeI CTNAG 4 cut(s) 273, 489, 656, 741
DpnI GATC 2 cut(s) 69, 115
DpnII GATC 2 cut(s) 67, 113
DraI TTTAAA 1 cut(s) 825
EciI GGCGGA 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 646
Eco32I GATATC 1 cut(s) 419
Eco81I CCTNAGG 1 cut(s) 741
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoRV GATATC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 646
EcoT22I ATGCAT 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 646
FaeI CATG 9 cut(s) 41, 107, 149, 209, 314, 507, 926, 959, 1049
FalI AAGNNNNNCTT 4 cut(s) 639, 671, 782, 814
FaqI GGGAC 3 cut(s) 267, 899, 1080
FatI CATG 9 cut(s) 37, 103, 145, 205, 310, 503, 922, 955, 1045
FauNDI CATATG 1 cut(s) 751
FblI GTMKAC 1 cut(s) 709
Fnu4HI GCNGC 2 cut(s) 278, 546
FokI GGATG 5 cut(s) 136, 167, 419, 456, 1022
Fsp4HI GCNGC 2 cut(s) 278, 546
FspBI CTAG 3 cut(s) 414, 431, 911
GluI GCNGC 2 cut(s) 278, 546
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 1 cut(s) 6
HgaI GACGC 1 cut(s) 1077
Hin1II CATG 9 cut(s) 41, 107, 149, 209, 314, 507, 926, 959, 1049
HinfI GANTC 4 cut(s) 178, 463, 610, 1013
HphI GGTGA 3 cut(s) 173, 689, 794
Hpy166II GTNNAC 1 cut(s) 710
Hpy188I TCNGA 8 cut(s) 118, 177, 301, 340, 492, 817, 838, 1003
Hpy188III TCNNGA 4 cut(s) 325, 392, 902, 1046
Hpy8I GTNNAC 1 cut(s) 710
HpyAV CCTTC 5 cut(s) 144, 277, 432, 543, 925
HpyCH4III ACNGT 2 cut(s) 185, 714
HpyCH4IV ACGT 2 cut(s) 853, 1005
HpyCH4V TGCA 4 cut(s) 507, 521, 548, 721
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 227, 386, 545, 554, 1030
HpyF3I CTNAG 4 cut(s) 273, 489, 656, 741
HpySE526I ACGT 2 cut(s) 853, 1005
Hsp92II CATG 9 cut(s) 41, 107, 149, 209, 314, 507, 926, 959, 1049
Kzo9I GATC 2 cut(s) 67, 113
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 2 cut(s) 264, 532
LweI GCATC 3 cut(s) 367, 397, 544
MaeI CTAG 3 cut(s) 414, 431, 911
MaeII ACGT 2 cut(s) 853, 1005
MaeIII GTNAC 4 cut(s) 607, 677, 734, 1060
MalI GATC 2 cut(s) 69, 115
MboI GATC 2 cut(s) 67, 113
MboII GAAGA 3 cut(s) 340, 609, 802
MfeI CAATTG 2 cut(s) 785, 807
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 2 cut(s) 232, 391
MluCI AATT 7 cut(s) 27, 50, 88, 726, 785, 807, 820
MlyI GAGTC 2 cut(s) 457, 604
Mph1103I ATGCAT 1 cut(s) 723
MroXI GAANNNNTTC 1 cut(s) 1053
MseI TTAA 6 cut(s) 291, 333, 824, 846, 941, 948
MslI CAYNNNNRTG 1 cut(s) 102
MunI CAATTG 2 cut(s) 785, 807
MwoI GCNNNNNNNGC 6 cut(s) 206, 227, 386, 545, 554, 1030
NdeI CATATG 1 cut(s) 751
NdeII GATC 2 cut(s) 67, 113
NlaIII CATG 9 cut(s) 41, 107, 149, 209, 314, 507, 926, 959, 1049
NlaIV GGNNCC 1 cut(s) 747
NmuCI GTSAC 2 cut(s) 607, 677
NsiI ATGCAT 1 cut(s) 723
PagI TCATGA 1 cut(s) 1045
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 2 cut(s) 178, 1013
PflFI GACNNNGTC 1 cut(s) 866
PkrI GCNGC 2 cut(s) 279, 547
PleI GAGTC 2 cut(s) 457, 604
PpsI GAGTC 2 cut(s) 457, 604
Ppu21I YACGTR 1 cut(s) 854
PspN4I GGNNCC 1 cut(s) 747
PspPI GGNCC 1 cut(s) 4
PsrI GAACNNNNNNTAC 2 cut(s) 626, 658
PsuI RGATCY 1 cut(s) 113
PsyI GACNNNGTC 1 cut(s) 866
RsaI GTAC 2 cut(s) 644, 766
RsaNI GTAC 2 cut(s) 643, 765
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 6 cut(s) 291, 333, 824, 846, 941, 948
SatI GCNGC 2 cut(s) 278, 546
Sau3AI GATC 2 cut(s) 67, 113
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 766
SchI GAGTC 2 cut(s) 457, 604
SduI GDGCHC 2 cut(s) 232, 391
SetI ASST 7 cut(s) 279, 856, 882, 891, 967, 1008, 1056
SfaNI GCATC 3 cut(s) 367, 397, 544
SfcI CTRYAG 1 cut(s) 858
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 3 cut(s) 390, 612, 666
SmoI CTYRAG 3 cut(s) 390, 612, 666
SpeI ACTAGT 1 cut(s) 430
Sse9I AATT 7 cut(s) 27, 50, 88, 726, 785, 807, 820
SsiI CCGC 2 cut(s) 280, 370
SspI AATATT 2 cut(s) 295, 497
SspMI CTAG 3 cut(s) 414, 431, 911
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 2 cut(s) 185, 714
TaiI ACGT 2 cut(s) 856, 1008
TaqI TCGA 1 cut(s) 236
TasI AATT 7 cut(s) 27, 50, 88, 726, 785, 807, 820
TatI WGTACW 1 cut(s) 764
TfiI GAWTC 2 cut(s) 178, 1013
Tru1I TTAA 6 cut(s) 291, 333, 824, 846, 941, 948
Tru9I TTAA 6 cut(s) 291, 333, 824, 846, 941, 948
TseFI GTSAC 2 cut(s) 607, 677
TseI GCWGC 2 cut(s) 277, 545
Tsp45I GTSAC 2 cut(s) 607, 677
TspDTI ATGAA 5 cut(s) 26, 321, 911, 972, 1062
Tth111I GACNNNGTC 1 cut(s) 866
XagI CCTNNNNNAGG 1 cut(s) 875
XapI RAATTY 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 709
XmnI GAANNNNTTC 1 cut(s) 1053
XspI CTAG 3 cut(s) 414, 431, 911
ZrmI AGTACT 1 cut(s) 766
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.