MD14G1008100.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005915
Physical Location & Seq
Forward (+)
832402 .. 838837
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1008100.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGGCAATCACAAGCACAACCATGGCATTGGATCTGAAAGGACGTCTCCAGCAACAAAACCTCATGGAAAGGATTTATGGGATATGGAGCTTCACATTCACCATTTGGAAACAGAGGCATACCATGCTGTTCTGAAGGCTTTTAGCGCTCAATCTAATAACCTAACTTGGGCACGGGAGGCACTTATGACTGAGCTGCGGAGGGAACTTAATATTACAGATGATGAACATGGACAGCTGCTCATTAAGATTAAGTCAGATCAGTCAATCAAAGTGATAAGGGAATGGCGGAATGGTGCCCCTTGTGCTCAAGAGTATAGTGAGGATGCTCCTAGATATCCTCCTCCTCAAGTAGTGATCAATGCTGAACCGAGAAAACTGGAGACTTCACATCCTCCTGTTTCAAAGTCTCAGAAATGTGTCTCATACAGTCATTCTTGTAAAGAAACTGCTCCTGCTGCACCAGATGCCCGCACCAAAAACACTAATGGAATGAAAGTCAAACGTGGAAAAAACTTAACTCAAGGACACAGCCTTGTCGTGAGCTTGAAGTACCAAGGACAACTTAGCAATGATAAAAGTACCGACCCGGCAATGCTCTCCTCTGAGAAAGGCAGGCAGGCTATGAATATAAGTTGTCATAATCCTCAGCCTCAGGTTCCATCTGGTAGCAGAAGTACTGGACTGGTAAAACCCCAATCGAAGATAGGTTTCCGCGCACCTGGCTCTGATAATTTCAAGGAAAGTTGTGATTTTATTAGGATTCGTGCTACAGATCAAGTTATTCATCAGGCTTATAAGCTGCTAAGTCAAGAAAGAGTAGTCCCACTTCATATAGAAAAGGCAAAGCTAATCCTTCGAGAGCACGAAAGAGCTATCCTCGACGCACTTGATAAACTGCCTGATGTGTCAGAGATGGCCGATTCTCCCAAACAAATGCAGCACAAGTATCCTCATGAGGAGCTTCCAGGAATGGGACGTGCAATGTTGATACGCAATGACTTGTATGGACAGGCTGGTAGGCTGGCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

345

Amino Acids

38.61

Weight (kDa)

9.12

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 32 - 99 2.1e-25 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
AatII GACGTC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 1 cut(s) 717
AciI CCGC 4 cut(s) 199, 289, 472, 715
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 918
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 3 cut(s) 554, 583, 679
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 2 cut(s) 169, 974
AgsI TTSAA 3 cut(s) 405, 550, 739
AjiI CACGTC 1 cut(s) 980
AjnI CCWGG 2 cut(s) 721, 967
AluBI AGCT 8 cut(s) 91, 196, 238, 546, 802, 850, 875, 964
AluI AGCT 8 cut(s) 91, 196, 238, 546, 802, 850, 875, 964
Alw21I GWGCWC 2 cut(s) 310, 867
Alw26I GTCTC 4 cut(s) 51, 377, 414, 427
AlwI GGATC 1 cut(s) 40
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 1 cut(s) 918
ApeKI GCWGC 5 cut(s) 196, 238, 458, 802, 940
AspLEI GCGC 2 cut(s) 149, 719
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 654
BaeGI GKGCMC 2 cut(s) 175, 301
BanI GGYRCC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 813, 845
Bbv12I GWGCWC 2 cut(s) 310, 867
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 5 cut(s) 183, 225, 445, 789, 952
BccI CCATC 2 cut(s) 670, 910
BciT130I CCWGG 2 cut(s) 723, 969
BciVI GTATCC 1 cut(s) 960
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 4 cut(s) 51, 377, 414, 427
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 1 cut(s) 960
BisI GCNGC 5 cut(s) 197, 239, 459, 803, 941
BlsI GCNGC 5 cut(s) 198, 240, 460, 804, 942
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 3 cut(s) 590, 723, 969
BmgBI CACGTC 1 cut(s) 980
BmiI GGNNCC 2 cut(s) 298, 660
BmrFI CCNGG 3 cut(s) 590, 723, 969
BmsI GCATC 2 cut(s) 316, 457
BplI GAGNNNNNCTC 2 cut(s) 864, 896
BpmI CTGGAG 2 cut(s) 33, 401
Bpu10I CCTNAGC 1 cut(s) 648
BpuEI CTTGAG 3 cut(s) 294, 333, 507
BpuMI CCSGG 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 22, 556
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 974
Bse1I ACTGG 3 cut(s) 384, 685, 690
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 4 cut(s) 577, 600, 990, 1003
BseBI CCWGG 2 cut(s) 723, 969
BseDI CCNNGG 2 cut(s) 22, 556
BseGI GGATG 2 cut(s) 331, 391
BseLI CCNNNNNNNGG 2 cut(s) 169, 974
BseMI GCAATG 4 cut(s) 577, 600, 990, 1003
BseMII CTCAG 5 cut(s) 183, 425, 597, 662, 668
BseNI ACTGG 3 cut(s) 384, 685, 690
BseRI GAGGAG 4 cut(s) 333, 336, 592, 974
BseSI GKGCMC 2 cut(s) 175, 301
BseXI GCAGC 5 cut(s) 183, 225, 445, 789, 952
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 717
BshFI GGCC 1 cut(s) 920
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 310, 867
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 2 cut(s) 809, 990
BslI CCNNNNNNNGG 2 cut(s) 169, 974
BsmAI GTCTC 4 cut(s) 51, 377, 414, 427
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 2 cut(s) 809, 990
BsnI GGCC 1 cut(s) 920
Bsp1286I GDGCHC 4 cut(s) 175, 301, 310, 867
Bsp143I GATC 4 cut(s) 32, 259, 357, 775
Bsp19I CCATGG 1 cut(s) 22
BspACI CCGC 4 cut(s) 199, 289, 472, 715
BspANI GGCC 1 cut(s) 920
BspCNI CTCAG 5 cut(s) 184, 424, 598, 661, 667
BspFNI CGCG 1 cut(s) 717
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 2 cut(s) 298, 660
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 4 cut(s) 577, 600, 990, 1003
BsrI ACTGG 3 cut(s) 384, 685, 690
BssECI CCNNGG 2 cut(s) 22, 556
BssMI GATC 4 cut(s) 32, 259, 357, 775
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 2 cut(s) 22, 556
Bst2UI CCWGG 2 cut(s) 723, 969
Bst4CI ACNGT 1 cut(s) 431
BstACI GRCGYC 1 cut(s) 44
BstAPI GCANNNNNTGC 2 cut(s) 125, 467
BstC8I GCNNGC 4 cut(s) 472, 617, 621, 1026
BstDEI CTNAG 7 cut(s) 192, 411, 566, 606, 648, 654, 806
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 2 cut(s) 331, 391
BstFNI CGCG 1 cut(s) 717
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 719
BstKTI GATC 4 cut(s) 35, 262, 360, 778
BstMAI GTCTC 4 cut(s) 51, 377, 414, 427
BstMBI GATC 4 cut(s) 32, 259, 357, 775
BstMWI GCNNNNNNNGC 7 cut(s) 125, 146, 179, 305, 458, 467, 723
BstNI CCWGG 2 cut(s) 723, 969
BstSCI CCNGG 3 cut(s) 588, 721, 967
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 2 cut(s) 175, 301
BstUI CGCG 1 cut(s) 717
BstV1I GCAGC 5 cut(s) 183, 225, 445, 789, 952
BstX2I RGATCY 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 1 cut(s) 32
Bsu36I CCTNAGG 1 cut(s) 654
BsuI GTATCC 1 cut(s) 960
BsuRI GGCC 1 cut(s) 920
BtgI CCRYGG 1 cut(s) 22
BtrI CACGTC 1 cut(s) 980
BtsCI GGATG 2 cut(s) 331, 391
Cac8I GCNNGC 4 cut(s) 472, 617, 621, 1026
CciI TCATGA 1 cut(s) 955
CfoI GCGC 2 cut(s) 149, 719
CseI GACGC 1 cut(s) 893
Csp6I GTAC 3 cut(s) 553, 582, 678
CviAII CATG 5 cut(s) 23, 65, 125, 230, 956
CviQI GTAC 3 cut(s) 553, 582, 678
DdeI CTNAG 7 cut(s) 192, 411, 566, 606, 648, 654, 806
DpnI GATC 4 cut(s) 34, 261, 359, 777
DpnII GATC 4 cut(s) 32, 259, 357, 775
EaeI YGGCCR 1 cut(s) 918
EciI GGCGGA 1 cut(s) 304
Eco130I CCWWGG 2 cut(s) 22, 556
Eco32I GATATC 1 cut(s) 338
Eco47III AGCGCT 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 654
EcoRII CCWGG 2 cut(s) 721, 967
EcoRV GATATC 1 cut(s) 338
EcoT14I CCWWGG 2 cut(s) 22, 556
ErhI CCWWGG 2 cut(s) 22, 556
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 5 cut(s) 26, 68, 128, 233, 959
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 2 cut(s) 809, 990
FatI CATG 5 cut(s) 22, 64, 124, 229, 955
FauI CCCGC 1 cut(s) 479
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 197, 239, 459, 803, 941
FokI GGATG 2 cut(s) 338, 378
Fsp4HI GCNGC 5 cut(s) 197, 239, 459, 803, 941
FspBI CTAG 1 cut(s) 333
GlaI GCGC 2 cut(s) 148, 718
GluI GCNGC 5 cut(s) 197, 239, 459, 803, 941
GsuI CTGGAG 2 cut(s) 33, 401
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 1 cut(s) 920
HapII CCGG 1 cut(s) 590
HgaI GACGC 1 cut(s) 893
HhaI GCGC 2 cut(s) 149, 719
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 5 cut(s) 26, 68, 128, 233, 959
Hin6I GCGC 2 cut(s) 147, 717
HinP1I GCGC 2 cut(s) 147, 717
HinfI GANTC 2 cut(s) 763, 923
HpaII CCGG 1 cut(s) 590
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 7 cut(s) 37, 135, 259, 414, 607, 730, 913
Hpy188III TCNNGA 5 cut(s) 311, 541, 812, 860, 956
Hpy99I CGWCG 1 cut(s) 887
HpyAV CCTTC 2 cut(s) 130, 866
HpyCH4III ACNGT 1 cut(s) 431
HpyCH4IV ACGT 3 cut(s) 44, 505, 979
HpyCH4V TGCA 3 cut(s) 461, 940, 983
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 146, 179, 305, 458, 467, 723
HpyF3I CTNAG 7 cut(s) 192, 411, 566, 606, 648, 654, 806
HpySE526I ACGT 3 cut(s) 44, 505, 979
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 5 cut(s) 26, 68, 128, 233, 959
HspAI GCGC 2 cut(s) 147, 717
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 4 cut(s) 32, 259, 357, 775
LmnI GCTCC 4 cut(s) 88, 334, 457, 961
Lsp1109I GCAGC 5 cut(s) 183, 225, 445, 789, 952
LweI GCATC 2 cut(s) 316, 457
MaeI CTAG 1 cut(s) 333
MaeII ACGT 3 cut(s) 44, 505, 979
MalI GATC 4 cut(s) 34, 261, 359, 777
MboI GATC 4 cut(s) 32, 259, 357, 775
MboII GAAGA 1 cut(s) 715
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 4 cut(s) 175, 301, 310, 867
MluCI AATT 1 cut(s) 733
MseI TTAA 4 cut(s) 210, 246, 252, 518
MslI CAYNNNNRTG 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 3 cut(s) 590, 723, 969
MvaI CCWGG 2 cut(s) 723, 969
MvnI CGCG 1 cut(s) 717
MwoI GCNNNNNNNGC 7 cut(s) 125, 146, 179, 305, 458, 467, 723
NciI CCSGG 1 cut(s) 590
NcoI CCATGG 1 cut(s) 22
NdeII GATC 4 cut(s) 32, 259, 357, 775
NlaIII CATG 5 cut(s) 26, 68, 128, 233, 959
NlaIV GGNNCC 2 cut(s) 298, 660
PagI TCATGA 1 cut(s) 955
PfeI GAWTC 2 cut(s) 763, 923
PfoI TCCNGGA 1 cut(s) 967
PkrI GCNGC 5 cut(s) 198, 240, 460, 804, 942
PsiI TTATAA 1 cut(s) 798
Psp6I CCWGG 2 cut(s) 721, 967
PspGI CCWGG 2 cut(s) 721, 967
PspN4I GGNNCC 2 cut(s) 298, 660
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 3 cut(s) 554, 583, 679
RsaNI GTAC 3 cut(s) 553, 582, 678
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 4 cut(s) 210, 246, 252, 518
SatI GCNGC 5 cut(s) 197, 239, 459, 803, 941
Sau3AI GATC 4 cut(s) 32, 259, 357, 775
ScaI AGTACT 1 cut(s) 679
ScrFI CCNGG 3 cut(s) 590, 723, 969
SduI GDGCHC 4 cut(s) 175, 301, 310, 867
SfaNI GCATC 2 cut(s) 316, 457
SfcI CTRYAG 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 3 cut(s) 309, 348, 522
SmoI CTYRAG 3 cut(s) 309, 348, 522
Sse9I AATT 1 cut(s) 733
SsiI CCGC 4 cut(s) 199, 289, 472, 715
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 588, 721, 967
StyI CCWWGG 2 cut(s) 22, 556
TaaI ACNGT 1 cut(s) 431
TaiI ACGT 3 cut(s) 47, 508, 982
TaqI TCGA 3 cut(s) 701, 859, 882
TasI AATT 1 cut(s) 733
TatI WGTACW 1 cut(s) 677
TfiI GAWTC 2 cut(s) 763, 923
Tru1I TTAA 4 cut(s) 210, 246, 252, 518
Tru9I TTAA 4 cut(s) 210, 246, 252, 518
TseI GCWGC 5 cut(s) 196, 238, 458, 802, 940
TspDTI ATGAA 5 cut(s) 240, 509, 641, 776, 821
XspI CTAG 1 cut(s) 333
ZraI GACGTC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.