RLG00000006938

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005915
Physical Location & Seq
Forward (+)
12032865 .. 12033465
601 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006938

Sequence Viewer

Length: 483 bp
ATGCGAGGTCGCAAACGCAAGTATTCTGGTGTTGCACCTTCAAGAGAAGCTCGTAGAGTCCGAAGGGGTGAAAGGAAAATTGACCATGAGATGATCCACCATATGGAAGCCGAGGCTTACCATGCTGTCTTGAAGGCCTTTAGTGCTCAATCTGATAGTATCTCTTGGGATCGGGTGGCGCTGATGACTAAGCTGCGGAAGGAACTTAACATTACAGATGATGAACATGGGGAGCTACTCGTGAAGATTAGGCAATCCGATGAGTCTATCAGAGCGTTAAGGGAATGGCGAAATTCGAATGGTACTGCTGCACAAGAGTTTGGTAATATCGGTGCTACGGATAAAATCATTCATGAGGTCGAGAAGCTGCTTGACAGTCAAGATATCCTCTCCCCTGCGCAATTGGAACGGGCAAACTTTGTCCTTCGAGAGCACGAAAGGGCTATCCTTGAAGCACTTGATCAACTGGCTATATATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

161

Amino Acids

18.43

Weight (kDa)

7.08

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 30 - 99 6.2e-25 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 399
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 1 cut(s) 196
AclWI GGATC 2 cut(s) 88, 177
AcsI RAATTY 1 cut(s) 292
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 3 cut(s) 42, 133, 452
AluBI AGCT 4 cut(s) 50, 193, 235, 367
AluI AGCT 4 cut(s) 50, 193, 235, 367
Alw21I GWGCWC 2 cut(s) 148, 435
AlwI GGATC 2 cut(s) 88, 177
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 3 cut(s) 193, 308, 367
ApoI RAATTY 1 cut(s) 292
AspLEI GCGC 2 cut(s) 181, 400
AsuHPI GGTGA 1 cut(s) 80
AsuII TTCGAA 1 cut(s) 296
BauI CACGAG 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 148, 435
BbvI GCAGC 3 cut(s) 180, 295, 354
BclI TGATCA 1 cut(s) 460
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 3 cut(s) 194, 309, 368
BlsI GCNGC 3 cut(s) 195, 310, 369
Bpu14I TTCGAA 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 471
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 471
BseXI GCAGC 3 cut(s) 180, 295, 354
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 148, 435
BslI CCNNNNNNNGG 1 cut(s) 103
BsnI GGCC 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 148, 435
Bsp143I GATC 3 cut(s) 93, 169, 460
BspACI CCGC 1 cut(s) 196
BspANI GGCC 1 cut(s) 137
BspHI TCATGA 1 cut(s) 352
BspPI GGATC 2 cut(s) 88, 177
BspT104I TTCGAA 1 cut(s) 296
BsrI ACTGG 1 cut(s) 471
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 93, 169, 460
BssSI CACGAG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 377
BstBI TTCGAA 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 2 cut(s) 181, 400
BstKTI GATC 3 cut(s) 96, 172, 463
BstMBI GATC 3 cut(s) 93, 169, 460
BstMWI GCNNNNNNNGC 2 cut(s) 122, 143
BstV1I GCAGC 3 cut(s) 180, 295, 354
BsuRI GGCC 1 cut(s) 137
CciI TCATGA 1 cut(s) 352
CfoI GCGC 2 cut(s) 181, 400
Csp6I GTAC 1 cut(s) 303
CviAII CATG 4 cut(s) 86, 122, 227, 353
CviJI RGCY 9 cut(s) 50, 110, 116, 137, 193, 235, 367, 443, 470
CviKI_1 RGCY 9 cut(s) 50, 110, 116, 137, 193, 235, 367, 443, 470
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 1 cut(s) 189
DpnI GATC 3 cut(s) 95, 171, 462
DpnII GATC 3 cut(s) 93, 169, 460
Eco147I AGGCCT 1 cut(s) 137
Eco32I GATATC 1 cut(s) 385
EcoRV GATATC 1 cut(s) 385
FaeI CATG 4 cut(s) 89, 125, 230, 356
FaiI YATR 9 cut(s) 87, 102, 104, 123, 228, 354, 473, 475, 477
FatI CATG 4 cut(s) 85, 121, 226, 352
FauNDI CATATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 3 cut(s) 194, 309, 368
Fsp4HI GCNGC 3 cut(s) 194, 309, 368
FspI TGCGCA 1 cut(s) 399
GlaI GCGC 2 cut(s) 180, 399
GluI GCNGC 3 cut(s) 194, 309, 368
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 1 cut(s) 137
HhaI GCGC 2 cut(s) 181, 400
Hin1II CATG 4 cut(s) 89, 125, 230, 356
Hin6I GCGC 2 cut(s) 179, 398
HinP1I GCGC 2 cut(s) 179, 398
HinfI GANTC 2 cut(s) 57, 263
HphI GGTGA 1 cut(s) 80
Hpy188I TCNGA 4 cut(s) 62, 154, 259, 272
Hpy188III TCNNGA 7 cut(s) 42, 130, 241, 353, 361, 380, 428
HpyAV CCTTC 5 cut(s) 48, 57, 127, 193, 434
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4V TGCA 2 cut(s) 35, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 143
HpyF3I CTNAG 1 cut(s) 189
Hsp92II CATG 4 cut(s) 89, 125, 230, 356
HspAI GCGC 2 cut(s) 179, 398
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 3 cut(s) 93, 169, 460
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 3 cut(s) 12, 408, 452
Lsp1109I GCAGC 3 cut(s) 180, 295, 354
MalI GATC 3 cut(s) 95, 171, 462
MboI GATC 3 cut(s) 93, 169, 460
MboII GAAGA 1 cut(s) 256
MfeI CAATTG 1 cut(s) 401
MhlI GDGCHC 2 cut(s) 148, 435
MluCI AATT 3 cut(s) 78, 292, 401
MlyI GAGTC 2 cut(s) 66, 272
MnlI CCTC 3 cut(s) 106, 349, 398
MseI TTAA 2 cut(s) 207, 278
MunI CAATTG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 122, 143
NdeI CATATG 1 cut(s) 102
NdeII GATC 3 cut(s) 93, 169, 460
NlaIII CATG 4 cut(s) 89, 125, 230, 356
NmeAIII GCCGAG 1 cut(s) 136
NsbI TGCGCA 1 cut(s) 399
NspV TTCGAA 1 cut(s) 296
PagI TCATGA 1 cut(s) 352
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 3 cut(s) 195, 310, 369
PleI GAGTC 2 cut(s) 65, 271
PpsI GAGTC 2 cut(s) 65, 271
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 207, 278
SatI GCNGC 3 cut(s) 194, 309, 368
Sau3AI GATC 3 cut(s) 93, 169, 460
SchI GAGTC 2 cut(s) 66, 272
SduI GDGCHC 2 cut(s) 148, 435
SetI ASST 7 cut(s) 10, 40, 52, 195, 237, 360, 369
SfuI TTCGAA 1 cut(s) 296
Sse9I AATT 3 cut(s) 78, 292, 401
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 1 cut(s) 196
StuI AGGCCT 1 cut(s) 137
TaaI ACNGT 1 cut(s) 377
TaqI TCGA 3 cut(s) 296, 360, 427
TasI AATT 3 cut(s) 78, 292, 401
Tru1I TTAA 2 cut(s) 207, 278
Tru9I TTAA 2 cut(s) 207, 278
TseI GCWGC 3 cut(s) 193, 308, 367
TspDTI ATGAA 2 cut(s) 237, 341
TspGWI ACGGA 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 103
XapI RAATTY 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.