FvH4_4g25570

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005433
Physical Location & Seq
Forward (+)
27294673 .. 27297655
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25570.t1

Sequence Viewer

Length: 606 bp
ATGGTCGGACCTTCAGCTGATCCCACCAAACTCGCTCTCAAATTCCTCTGCAGCTACAGCGGCAAAATCCTCCCCCGTTACCCCGACGGCAAGCTCCGTTATCTCGGCGGCGAAACCCGCGTCCTTGCCGTCCACCGCTCCATTTCCTTCTCTGAGCTATTGTTGAAGCTCGGAGAGCTGTGTGGCACAACCGTCGGTCTCCGTTGCCAACTGCCAACCGAAGACCTCGACGCCCTTATCTCCATAAAGTGCGACGAAGACCTCGCCAATCTCATCGAGGAGTACGACAGAGCTGCGGCGGCCGCCACGCCGTTGAAGATCAGGGCCTTCCTTTCGCTCCCCGTCAACAAAACTACGTCGTCTCCTCCGTCTTCCTCGGCCTCTTCCTCGAAATCCTCGTCTTCCAATCACAGTACAACCTGTGCCTCCGGATCCACTCCCAGATTCTCCCTGCCTTTGCCGGCGGAGCGTTGCCTCCGTCAAGTTTCGCATTCGGCGCCGTTTCAGCGAGCTCCAGCTGGGAAAGCTCCTCACTGTGATAATTACCACACTCATAGAAACCCTAGCCGTCTCTATCTCGTCCACAACGGAAACCACTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

202

Amino Acids

21.79

Weight (kDa)

9.14

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 29 - 98 2.1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 496
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 428
AciI CCGC 8 cut(s) 60, 108, 118, 136, 296, 299, 303, 464
AclWI GGATC 3 cut(s) 14, 426, 439
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 41
AcyI GRCGYC 2 cut(s) 231, 497
AfaI GTAC 2 cut(s) 284, 415
AgsI TTSAA 2 cut(s) 166, 316
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 3 cut(s) 203, 366, 575
AlwI GGATC 3 cut(s) 14, 426, 439
Aor13HI TCCGGA 1 cut(s) 428
AoxI GGCC 3 cut(s) 300, 324, 378
ApeKI GCWGC 2 cut(s) 51, 293
ApoI RAATTY 1 cut(s) 41
ArsI GACNNNNNNTTYG 4 cut(s) 105, 137, 383, 415
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 2 cut(s) 8, 324
AvaII GGWCC 1 cut(s) 8
BamHI GGATCC 1 cut(s) 431
BanI GGYRCC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 514
BbsI GAAGAC 4 cut(s) 228, 264, 363, 393
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 2 cut(s) 63, 280
BceAI ACGGC 5 cut(s) 103, 113, 295, 484, 552
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 275, 309
BcoDI GTCTC 3 cut(s) 203, 366, 575
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 2 cut(s) 49, 55
BfoI RGCGCY 1 cut(s) 500
BisI GCNGC 7 cut(s) 52, 61, 109, 294, 297, 300, 303
BlsI GCNGC 7 cut(s) 53, 62, 110, 295, 298, 301, 304
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 8, 324
BmiI GGNNCC 2 cut(s) 433, 498
BpiI GAAGAC 4 cut(s) 228, 264, 363, 393
BpmI CTGGAG 1 cut(s) 498
BsaHI GRCGYC 2 cut(s) 231, 497
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 375
BsaWI WCCGGW 1 cut(s) 428
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 602
BseAI TCCGGA 1 cut(s) 428
BseDI CCNNGG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 602
BseRI GAGGAG 3 cut(s) 293, 354, 519
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 2 cut(s) 63, 280
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 3 cut(s) 302, 326, 380
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 514
BsiSI CCGG 2 cut(s) 429, 461
BsmAI GTCTC 3 cut(s) 203, 366, 575
BsmBI CGTCTC 2 cut(s) 366, 575
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 3 cut(s) 302, 326, 380
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 514
Bsp13I TCCGGA 1 cut(s) 428
Bsp143I GATC 3 cut(s) 19, 318, 431
BspACI CCGC 8 cut(s) 60, 108, 118, 136, 296, 299, 303, 464
BspANI GGCC 3 cut(s) 302, 326, 380
BspCNI CTCAG 1 cut(s) 145
BspEI TCCGGA 1 cut(s) 428
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 433, 498
BspMAI CTGCAG 1 cut(s) 53
BspPI GGATC 3 cut(s) 14, 426, 439
BspT107I GGYRCC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 602
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 3 cut(s) 19, 318, 431
BssNI GRCGYC 2 cut(s) 231, 497
Bst4CI ACNGT 3 cut(s) 193, 413, 536
Bst6I CTCTTC 1 cut(s) 388
BstACI GRCGYC 2 cut(s) 231, 497
BstC8I GCNNGC 3 cut(s) 92, 462, 510
BstDEI CTNAG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 3 cut(s) 22, 321, 434
BstMAI GTCTC 3 cut(s) 203, 366, 575
BstMBI GATC 3 cut(s) 19, 318, 431
BstMCI CGRYCG 1 cut(s) 303
BstSFI CTRYAG 2 cut(s) 49, 55
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 63, 280
BstV2I GAAGAC 4 cut(s) 228, 264, 363, 393
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BstZI CGGCCG 1 cut(s) 300
BsuRI GGCC 3 cut(s) 302, 326, 380
BtsIMutI CAGTG 2 cut(s) 532, 595
Cac8I GCNNGC 3 cut(s) 92, 462, 510
CciNI GCGGCCGC 1 cut(s) 300
CfoI GCGC 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 8, 324
CseI GACGC 2 cut(s) 109, 239
Csp6I GTAC 2 cut(s) 283, 414
CviQI GTAC 2 cut(s) 283, 414
DdeI CTNAG 1 cut(s) 153
DinI GGCGCC 1 cut(s) 498
DpnI GATC 3 cut(s) 21, 320, 433
DpnII GATC 3 cut(s) 19, 318, 431
EaeI YGGCCR 1 cut(s) 300
EagI CGGCCG 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
EciI GGCGGA 1 cut(s) 479
Ecl136II GAGCTC 1 cut(s) 512
EclXI CGGCCG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 514
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 8
Eco52I CGGCCG 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 512
EcoO109I RGGNCCY 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 514
EgeI GGCGCC 1 cut(s) 498
EheI GGCGCC 1 cut(s) 498
Esp3I CGTCTC 2 cut(s) 366, 575
FaiI YATR 2 cut(s) 245, 555
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 7 cut(s) 52, 61, 109, 294, 297, 300, 303
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 7 cut(s) 52, 61, 109, 294, 297, 300, 303
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 498
GluI GCNGC 7 cut(s) 52, 61, 109, 294, 297, 300, 303
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 3 cut(s) 302, 326, 380
HapII CCGG 2 cut(s) 429, 461
HgaI GACGC 2 cut(s) 109, 239
HhaI GCGC 1 cut(s) 499
Hin1I GRCGYC 2 cut(s) 231, 497
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 1 cut(s) 444
HpaII CCGG 2 cut(s) 429, 461
Hpy166II GTNNAC 3 cut(s) 133, 346, 583
Hpy188I TCNGA 3 cut(s) 8, 154, 173
Hpy188III TCNNGA 1 cut(s) 429
Hpy8I GTNNAC 3 cut(s) 133, 346, 583
Hpy99I CGWCG 5 cut(s) 89, 197, 233, 257, 361
HpyAV CCTTC 3 cut(s) 21, 157, 337
HpyCH4III ACNGT 3 cut(s) 193, 413, 536
HpyCH4IV ACGT 1 cut(s) 356
HpyCH4V TGCA 1 cut(s) 51
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 1 cut(s) 356
Hsp92I GRCGYC 2 cut(s) 231, 497
HspAI GCGC 1 cut(s) 497
KasI GGCGCC 1 cut(s) 496
Kpn2I TCCGGA 1 cut(s) 428
KroI GCCGGC 1 cut(s) 460
KroNI GCCGGC 1 cut(s) 462
Kzo9I GATC 3 cut(s) 19, 318, 431
LmnI GCTCC 6 cut(s) 99, 143, 342, 466, 517, 532
LpnPI CCDG 9 cut(s) 307, 433, 442, 454, 464, 474, 504, 528, 583
Lsp1109I GCAGC 2 cut(s) 63, 280
MaeI CTAG 1 cut(s) 564
MaeII ACGT 1 cut(s) 356
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 21, 320, 433
MbiI CCGCTC 1 cut(s) 138
MboI GATC 3 cut(s) 19, 318, 431
MboII GAAGA 6 cut(s) 233, 269, 328, 363, 375, 393
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 2 cut(s) 41, 541
Mly113I GGCGCC 1 cut(s) 497
MroI TCCGGA 1 cut(s) 428
MroNI GCCGGC 1 cut(s) 460
MspA1I CMGCKG 3 cut(s) 17, 60, 518
MspI CCGG 2 cut(s) 429, 461
Mva1269I GAATGC 1 cut(s) 490
MvnI CGCG 1 cut(s) 120
NaeI GCCGGC 1 cut(s) 462
NarI GGCGCC 1 cut(s) 497
NdeII GATC 3 cut(s) 19, 318, 431
NgoMIV GCCGGC 1 cut(s) 460
NlaIV GGNNCC 2 cut(s) 433, 498
NmeAIII GCCGAG 2 cut(s) 84, 356
NotI GCGGCCGC 1 cut(s) 300
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 365, 395
PctI GAATGC 1 cut(s) 490
PdiI GCCGGC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 444
PkrI GCNGC 7 cut(s) 53, 62, 110, 295, 298, 301, 304
PluTI GGCGCC 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 518
PspN4I GGNNCC 2 cut(s) 433, 498
PspPI GGNCC 2 cut(s) 8, 324
PstI CTGCAG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 431
PvuII CAGCTG 2 cut(s) 17, 518
RsaI GTAC 2 cut(s) 284, 415
RsaNI GTAC 2 cut(s) 283, 414
SacI GAGCTC 1 cut(s) 514
SatI GCNGC 7 cut(s) 52, 61, 109, 294, 297, 300, 303
Sau3AI GATC 3 cut(s) 19, 318, 431
Sau96I GGNCC 2 cut(s) 8, 324
SduI GDGCHC 1 cut(s) 514
SfcI CTRYAG 2 cut(s) 49, 55
SfoI GGCGCC 1 cut(s) 498
SinI GGWCC 1 cut(s) 8
Sse9I AATT 2 cut(s) 41, 541
SsiI CCGC 8 cut(s) 60, 108, 118, 136, 296, 299, 303, 464
SspDI GGCGCC 1 cut(s) 496
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 1 cut(s) 514
TaaI ACNGT 3 cut(s) 193, 413, 536
TaiI ACGT 1 cut(s) 359
TaqI TCGA 3 cut(s) 228, 276, 389
TaqII GACCGA 1 cut(s) 185
TasI AATT 2 cut(s) 41, 541
TatI WGTACW 1 cut(s) 413
TauI GCSGC 5 cut(s) 63, 111, 299, 302, 305
TfiI GAWTC 1 cut(s) 444
TscAI CASTG 2 cut(s) 539, 602
TseI GCWGC 2 cut(s) 51, 293
TspGWI ACGGA 5 cut(s) 86, 191, 357, 467, 603
TspRI CASTG 2 cut(s) 539, 602
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 1 cut(s) 41
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.