Prupe.1G244500_v2.0.a1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005433
Physical Location & Seq
Forward (+)
25788397 .. 25792336
3940 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G244500.3

Sequence Viewer

Length: 669 bp
ATGGTAGGACCCTCGCCTGACCCCACCAAGCCCTCCACCATCAAGTTCCTCTGCAGCTACGGTGGCAAAATCCTCCCCCGTTATCCCGACGGCAAGCTTCGTTATCTCGGCGGCGAAACCCGCGTCCTCGCCGTCCACCGCAACATTTCCTTTTCCGAGCTGTTGTTGAAGCTGGGTGAGCTGTGTGGGCCCTCCGTGACCGTGAGCCTACGTTGTCAGTTGCCCACCGAGGATCTCGACGCCCTTGTGTCCATCAAGTCCGATGAGGATCTGGCCAATCTCATCGAAGAATACGACAGAGCAGCCGCCTCTGCATCTCCACCGCCTTTTGCCAATTTGAAGATCAGAGCCTTCCTTTCCCTCGTCCCTCCCAAACCACACAAAACGCCGTCGTCTACGCCGTCTTCCTCGGCCTCCGCCTCCACGACGTCGTCCTCCGGCACTTCCTCCTCGTCTAACTACTACTACAGCGCTGCTGCTTCCGGGTCCACTCCCAGGTTCCCCATGGTGTCCACGCCAGTTGACCGCTGTGTCCGCCAAATCTCACAGGCTACGACGCCGTTTCAGAAATCAGCCGCCAAGGTTCCTCACTGTGCTAATATTTGCCATGCTCATGGAAGCCCTAGTAGTCGGCTCTATCTCATTCACAACGGCAACCACTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

223

Amino Acids

23.75

Weight (kDa)

9.17

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 530
AatII GACGTC 1 cut(s) 431
AccI GTMKAC 1 cut(s) 395
AccII CGCG 1 cut(s) 123
AciI CCGC 9 cut(s) 111, 121, 139, 306, 323, 417, 526, 535, 576
AclWI GGATC 2 cut(s) 240, 276
AcoI YGGCCR 1 cut(s) 273
AcyI GRCGYC 3 cut(s) 240, 428, 557
AfeI AGCGCT 1 cut(s) 472
AfiI CCNNNNNNNGG 1 cut(s) 495
AgsI TTSAA 2 cut(s) 169, 340
AjnI CCWGG 1 cut(s) 494
AluBI AGCT 5 cut(s) 57, 97, 160, 172, 181
AluI AGCT 5 cut(s) 57, 97, 160, 172, 181
AlwI GGATC 2 cut(s) 240, 276
Aor51HI AGCGCT 1 cut(s) 472
AoxI GGCC 3 cut(s) 188, 273, 411
ApaI GGGCCC 1 cut(s) 192
ApeKI GCWGC 4 cut(s) 54, 302, 473, 476
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspLEI GCGC 1 cut(s) 473
AspS9I GGNCC 4 cut(s) 8, 188, 189, 486
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 2 cut(s) 8, 486
BaeGI GKGCMC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 275
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 396
BbvI GCAGC 4 cut(s) 66, 314, 460, 463
BccI CCATC 2 cut(s) 47, 260
BceAI ACGGC 6 cut(s) 106, 116, 373, 385, 544, 667
BciT130I CCWGG 1 cut(s) 496
BcnI CCSGG 1 cut(s) 484
BfaI CTAG 1 cut(s) 624
BfmI CTRYAG 2 cut(s) 52, 466
BfoI RGCGCY 1 cut(s) 474
BisI GCNGC 7 cut(s) 55, 112, 303, 306, 474, 477, 576
BlsI GCNGC 7 cut(s) 56, 113, 304, 307, 475, 478, 577
Bme1390I CCNGG 2 cut(s) 484, 496
Bme18I GGWCC 2 cut(s) 8, 486
BmgT120I GGNCC 4 cut(s) 8, 188, 189, 486
BmiI GGNNCC 5 cut(s) 10, 190, 487, 500, 585
BmrFI CCNGG 2 cut(s) 484, 496
BmsI GCATC 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 484
BsaBI GATNNNNATC 1 cut(s) 267
BsaHI GRCGYC 3 cut(s) 240, 428, 557
BsaJI CCNNGG 5 cut(s) 228, 408, 494, 504, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 495
Bse1I ACTGG 2 cut(s) 518, 665
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 1 cut(s) 496
BseDI CCNNGG 5 cut(s) 228, 408, 494, 504, 579
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 495
BseNI ACTGG 2 cut(s) 518, 665
BseRI GAGGAG 1 cut(s) 439
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 4 cut(s) 66, 314, 460, 463
BseYI CCCAGC 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 123
BshFI GGCC 3 cut(s) 190, 275, 413
BsiSI CCGG 2 cut(s) 438, 483
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 495
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 3 cut(s) 190, 275, 413
Bsp120I GGGCCC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 232, 268, 342
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 9 cut(s) 111, 121, 139, 306, 323, 417, 526, 535, 576
BspANI GGCC 3 cut(s) 190, 275, 413
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 5 cut(s) 10, 190, 487, 500, 585
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 240, 276
BsrI ACTGG 2 cut(s) 518, 665
BssECI CCNNGG 5 cut(s) 228, 408, 494, 504, 579
BssMI GATC 3 cut(s) 232, 268, 342
BssNI GRCGYC 3 cut(s) 240, 428, 557
BssT1I CCWWGG 2 cut(s) 504, 579
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 3 cut(s) 62, 202, 593
BstACI GRCGYC 3 cut(s) 240, 428, 557
BstC8I GCNNGC 1 cut(s) 95
BstDSI CCRYGG 1 cut(s) 504
BstFNI CGCG 1 cut(s) 123
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 1 cut(s) 473
BstKTI GATC 3 cut(s) 235, 271, 345
BstMBI GATC 3 cut(s) 232, 268, 342
BstMWI GCNNNNNNNGC 6 cut(s) 63, 120, 178, 187, 311, 534
BstNI CCWGG 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 482, 494
BstSFI CTRYAG 2 cut(s) 52, 466
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 4 cut(s) 66, 314, 460, 463
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 2 cut(s) 232, 268
BstXI CCANNNNNNTGG 1 cut(s) 614
BstYI RGATCY 2 cut(s) 232, 268
BsuRI GGCC 3 cut(s) 190, 275, 413
BtgI CCRYGG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 589, 658
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 1 cut(s) 473
Cfr13I GGNCC 4 cut(s) 8, 188, 189, 486
CseI GACGC 3 cut(s) 112, 248, 565
CspCI CAANNNNNGTGG 2 cut(s) 502, 537
CviAII CATG 3 cut(s) 505, 608, 614
DpnI GATC 3 cut(s) 234, 270, 344
DpnII GATC 3 cut(s) 232, 268, 342
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 1 cut(s) 273
EciI GGCGGA 2 cut(s) 406, 524
Eco130I CCWWGG 2 cut(s) 504, 579
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 2 cut(s) 8, 486
Eco47III AGCGCT 1 cut(s) 472
EcoO109I RGGNCCY 2 cut(s) 8, 189
EcoRII CCWGG 1 cut(s) 494
EcoT14I CCWWGG 2 cut(s) 504, 579
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 2 cut(s) 504, 579
FaeI CATG 3 cut(s) 508, 611, 617
FaiI YATR 3 cut(s) 506, 609, 615
FaqI GGGAC 1 cut(s) 350
FatI CATG 3 cut(s) 504, 607, 613
FauI CCCGC 1 cut(s) 128
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 7 cut(s) 55, 112, 303, 306, 474, 477, 576
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 7 cut(s) 55, 112, 303, 306, 474, 477, 576
FspBI CTAG 1 cut(s) 624
GlaI GCGC 1 cut(s) 472
GluI GCNGC 7 cut(s) 55, 112, 303, 306, 474, 477, 576
GsaI CCCAGC 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 3 cut(s) 190, 275, 413
HapII CCGG 2 cut(s) 438, 483
HgaI GACGC 3 cut(s) 112, 248, 565
HhaI GCGC 1 cut(s) 473
Hin1I GRCGYC 3 cut(s) 240, 428, 557
Hin1II CATG 3 cut(s) 508, 611, 617
Hin6I GCGC 1 cut(s) 471
HinP1I GCGC 1 cut(s) 471
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 95
HpaII CCGG 2 cut(s) 438, 483
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 5 cut(s) 136, 396, 489, 513, 523
Hpy188I TCNGA 4 cut(s) 157, 262, 347, 567
Hpy188III TCNNGA 2 cut(s) 86, 236
Hpy8I GTNNAC 5 cut(s) 136, 396, 489, 513, 523
Hpy99I CGWCG 6 cut(s) 92, 242, 394, 430, 433, 559
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 3 cut(s) 62, 202, 593
HpyCH4IV ACGT 2 cut(s) 211, 428
HpyCH4V TGCA 2 cut(s) 54, 314
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 120, 178, 187, 311, 534
HpySE526I ACGT 2 cut(s) 211, 428
Hsp92I GRCGYC 3 cut(s) 240, 428, 557
Hsp92II CATG 3 cut(s) 508, 611, 617
HspAI GCGC 1 cut(s) 471
Kzo9I GATC 3 cut(s) 232, 268, 342
Lsp1109I GCAGC 4 cut(s) 66, 314, 460, 463
LweI GCATC 1 cut(s) 323
MaeI CTAG 1 cut(s) 624
MaeII ACGT 2 cut(s) 211, 428
MaeIII GTNAC 1 cut(s) 196
MalI GATC 3 cut(s) 234, 270, 344
MboI GATC 3 cut(s) 232, 268, 342
MboII GAAGA 3 cut(s) 299, 352, 396
MflI RGATCY 2 cut(s) 232, 268
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 1 cut(s) 334
MluNI TGGCCA 1 cut(s) 275
Mox20I TGGCCA 1 cut(s) 275
MscI TGGCCA 1 cut(s) 275
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 528
MspI CCGG 2 cut(s) 438, 483
MspR9I CCNGG 2 cut(s) 484, 496
MvaI CCWGG 1 cut(s) 496
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 6 cut(s) 63, 120, 178, 187, 311, 534
NciI CCSGG 1 cut(s) 484
NcoI CCATGG 1 cut(s) 504
NdeII GATC 3 cut(s) 232, 268, 342
NlaIII CATG 3 cut(s) 508, 611, 617
NlaIV GGNNCC 5 cut(s) 10, 190, 487, 500, 585
NmeAIII GCCGAG 2 cut(s) 87, 389
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 398
PflFI GACNNNGTC 1 cut(s) 430
PkrI GCNGC 7 cut(s) 56, 113, 304, 307, 475, 478, 577
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 1 cut(s) 494
PspN4I GGNNCC 5 cut(s) 10, 190, 487, 500, 585
PspOMI GGGCCC 1 cut(s) 188
PspPI GGNCC 4 cut(s) 8, 188, 189, 486
PspPPI RGGWCCY 1 cut(s) 8
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 2 cut(s) 232, 268
PsyI GACNNNGTC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 612
SatI GCNGC 7 cut(s) 55, 112, 303, 306, 474, 477, 576
Sau3AI GATC 3 cut(s) 232, 268, 342
Sau96I GGNCC 4 cut(s) 8, 188, 189, 486
ScrFI CCNGG 2 cut(s) 484, 496
SduI GDGCHC 1 cut(s) 192
SetI ASST 9 cut(s) 59, 99, 162, 174, 183, 214, 431, 500, 585
SfaNI GCATC 1 cut(s) 323
SfcI CTRYAG 2 cut(s) 52, 466
SinI GGWCC 2 cut(s) 8, 486
SmiMI CAYNNNNRTG 1 cut(s) 612
Sse9I AATT 1 cut(s) 334
SsiI CCGC 9 cut(s) 111, 121, 139, 306, 323, 417, 526, 535, 576
SspI AATATT 1 cut(s) 601
SspMI CTAG 1 cut(s) 624
StyD4I CCNGG 2 cut(s) 482, 494
StyI CCWWGG 2 cut(s) 504, 579
TaaI ACNGT 3 cut(s) 62, 202, 593
TaiI ACGT 2 cut(s) 214, 431
TaqI TCGA 2 cut(s) 237, 285
TasI AATT 1 cut(s) 334
TauI GCSGC 3 cut(s) 114, 308, 578
TscAI CASTG 2 cut(s) 596, 665
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 4 cut(s) 54, 302, 473, 476
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 2 cut(s) 596, 665
Tth111I GACNNNGTC 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 8, 486
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 395
XspI CTAG 1 cut(s) 624
ZraI GACGTC 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.