pycom13g08840

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005433
Physical Location & Seq
Reverse (-)
5898491 .. 5899276
786 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g08840.1

Sequence Viewer

Length: 699 bp
ATGGTCGGACCCTCGCCTGACCTCACCAAGGTTACCATCATCAAATTCCTCTGCAGCTACGGCGGCAAAATCCTCCCCCGTTACCCCGACGGCAAGCTCCGTTACCTTGGCGGCGAAACCCGTGTACTCGCCGTTCCCCGCTCTATTTCCTTTTCCGAGCTGTCGTCCAAGCTGACTGAGTTGTGTGGGCCATCCGTGACGGCGGTTAGTCTCCGCTGTCAACTACCCACCGAAGATCTCGACGCCCTCGTGTCCATCAAGTCCGATGAGGATCTCGTTAATCTCATCGAGGAATACGACAGAGCAGCCTCTTCCGCTAATATGAAGATCAGAGCATTCCTCTCACTCATCCCCACCGCCAGGACACCCAAAAGCATTTCATCCCCTTCAACTTCCTCGGCCTCCGCCGCCTCCACGACGTCGTCCTCCGGCAACTCCTCGTCGTCAAATAACTACTTCAGTGCGGCTTCCGGGTCCACCCCAAGATTTCCCATGCCGTCCTCCACTCCCGTTGACCGTTGCCTCCGTCAAATGCCGCCCCAGCCATCCCCGTCAACGGTGCCGTTTCAGAGACCCCCCTCCAAGGTTCCTCACTGTGCTAATATTTACCATGCTCGCCAAGGCCACGGAAGCAACAGACCTGCAAGTACTACCGGTAGTCGTCCCTACCTCGTTCACAACGGCAATCACTGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

233

Amino Acids

24.85

Weight (kDa)

9.53

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 30 - 110 8.9e-18 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 422
Acc36I ACCTGC 1 cut(s) 649
AccB1I GGYRCC 1 cut(s) 559
AccBSI CCGCTC 1 cut(s) 141
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 442
AcyI GRCGYC 2 cut(s) 243, 419
AfaI GTAC 2 cut(s) 126, 649
AfiI CCNNNNNNNGG 4 cut(s) 28, 360, 556, 583
AgeI ACCGGT 1 cut(s) 653
AgsI TTSAA 1 cut(s) 390
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 4 cut(s) 57, 97, 160, 172
AluI AGCT 4 cut(s) 57, 97, 160, 172
Alw26I GTCTC 2 cut(s) 215, 565
AlwI GGATC 1 cut(s) 279
AoxI GGCC 3 cut(s) 188, 399, 622
ApeKI GCWGC 2 cut(s) 54, 305
ApoI RAATTY 1 cut(s) 44
AsiGI ACCGGT 1 cut(s) 653
AspS9I GGNCC 3 cut(s) 8, 188, 474
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 8, 474
BanI GGYRCC 1 cut(s) 559
BauI CACGAG 1 cut(s) 248
BbvI GCAGC 2 cut(s) 66, 317
BccI CCATC 4 cut(s) 44, 199, 263, 553
BceAI ACGGC 7 cut(s) 76, 106, 116, 216, 481, 547, 697
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 2 cut(s) 215, 565
BfmI CTRYAG 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 649
BglII AGATCT 1 cut(s) 235
BisI GCNGC 7 cut(s) 55, 64, 112, 306, 408, 465, 536
BlsI GCNGC 7 cut(s) 56, 65, 113, 307, 409, 466, 537
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 361, 472
Bme18I GGWCC 2 cut(s) 8, 474
BmgT120I GGNCC 3 cut(s) 8, 188, 474
BmiI GGNNCC 4 cut(s) 10, 475, 561, 588
BmrFI CCNGG 2 cut(s) 361, 472
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpuMI CCSGG 1 cut(s) 472
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 2 cut(s) 243, 419
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 6 cut(s) 27, 106, 396, 582, 619, 625
BsaWI WCCGGW 1 cut(s) 653
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 360, 556, 583
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 1 cut(s) 695
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 6 cut(s) 27, 106, 396, 582, 619, 625
BseGI GGATG 4 cut(s) 191, 348, 380, 545
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 4 cut(s) 28, 360, 556, 583
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 695
BseRI GAGGAG 1 cut(s) 427
BseXI GCAGC 2 cut(s) 66, 317
BseYI CCCAGC 1 cut(s) 540
BshFI GGCC 3 cut(s) 190, 401, 624
BshNI GGYRCC 1 cut(s) 559
BshTI ACCGGT 1 cut(s) 653
BsiSI CCGG 3 cut(s) 429, 471, 654
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 4 cut(s) 28, 360, 556, 583
BsmAI GTCTC 2 cut(s) 215, 565
BsmFI GGGAC 1 cut(s) 648
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 3 cut(s) 190, 401, 624
Bso31I GGTCTC 1 cut(s) 565
Bsp143I GATC 3 cut(s) 235, 271, 327
BspANI GGCC 3 cut(s) 190, 401, 624
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 4 cut(s) 10, 475, 561, 588
BspMAI CTGCAG 1 cut(s) 56
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 1 cut(s) 695
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 6 cut(s) 27, 106, 396, 582, 619, 625
BssMI GATC 3 cut(s) 235, 271, 327
BssNI GRCGYC 2 cut(s) 243, 419
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 4 cut(s) 27, 106, 582, 619
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 3 cut(s) 518, 559, 596
Bst6I CTCTTC 1 cut(s) 316
BstACI GRCGYC 2 cut(s) 243, 419
BstC8I GCNNGC 2 cut(s) 95, 616
BstDEI CTNAG 1 cut(s) 177
BstDSI CCRYGG 1 cut(s) 625
BstEII GGTNACC 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 26
BstF5I GGATG 4 cut(s) 191, 348, 380, 545
BstKTI GATC 3 cut(s) 238, 274, 330
BstMAI GTCTC 2 cut(s) 215, 565
BstMBI GATC 3 cut(s) 235, 271, 327
BstMWI GCNNNNNNNGC 6 cut(s) 60, 63, 314, 407, 541, 630
BstNI CCWGG 1 cut(s) 361
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 359, 470
BstSFI CTRYAG 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 66, 317
BstX2I RGATCY 2 cut(s) 235, 271
BstYI RGATCY 2 cut(s) 235, 271
BsuRI GGCC 3 cut(s) 190, 401, 624
BtgI CCRYGG 1 cut(s) 625
BtsCI GGATG 4 cut(s) 191, 348, 380, 545
BtsIMutI CAGTG 3 cut(s) 466, 592, 688
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 2 cut(s) 95, 616
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 3 cut(s) 8, 188, 474
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 125, 648
CspAI ACCGGT 1 cut(s) 653
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CviAII CATG 2 cut(s) 493, 611
CviQI GTAC 2 cut(s) 125, 648
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 237, 273, 329
DpnII GATC 3 cut(s) 235, 271, 327
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EciI GGCGGA 1 cut(s) 394
Eco130I CCWWGG 4 cut(s) 27, 106, 582, 619
Eco31I GGTCTC 1 cut(s) 565
Eco47I GGWCC 2 cut(s) 8, 474
Eco57I CTGAAG 1 cut(s) 442
Eco91I GGTNACC 1 cut(s) 31
EcoNI CCTNNNNNAGG 1 cut(s) 26
EcoO65I GGTNACC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 359
EcoT14I CCWWGG 4 cut(s) 27, 106, 582, 619
ErhI CCWWGG 4 cut(s) 27, 106, 582, 619
FaeI CATG 2 cut(s) 496, 614
FaiI YATR 3 cut(s) 323, 494, 612
FaqI GGGAC 1 cut(s) 648
FatI CATG 2 cut(s) 492, 610
FauI CCCGC 1 cut(s) 146
Fnu4HI GCNGC 7 cut(s) 55, 64, 112, 306, 408, 465, 536
FokI GGATG 4 cut(s) 178, 335, 367, 532
Fsp4HI GCNGC 7 cut(s) 55, 64, 112, 306, 408, 465, 536
GluI GCNGC 7 cut(s) 55, 64, 112, 306, 408, 465, 536
GsaI CCCAGC 1 cut(s) 544
HaeIII GGCC 3 cut(s) 190, 401, 624
HapII CCGG 3 cut(s) 429, 471, 654
HgaI GACGC 1 cut(s) 251
Hin1I GRCGYC 2 cut(s) 243, 419
Hin1II CATG 2 cut(s) 496, 614
HincII GTYRAC 3 cut(s) 221, 514, 555
HindII GTYRAC 3 cut(s) 221, 514, 555
HpaII CCGG 3 cut(s) 429, 471, 654
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 6 cut(s) 125, 221, 477, 514, 555, 676
Hpy188I TCNGA 5 cut(s) 8, 157, 265, 332, 570
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 6 cut(s) 125, 221, 477, 514, 555, 676
Hpy99I CGWCG 5 cut(s) 92, 245, 421, 424, 445
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 3 cut(s) 518, 559, 596
HpyCH4IV ACGT 1 cut(s) 419
HpyCH4V TGCA 2 cut(s) 54, 644
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 63, 314, 407, 541, 630
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 419
Hsp92I GRCGYC 2 cut(s) 243, 419
Hsp92II CATG 2 cut(s) 496, 614
Kzo9I GATC 3 cut(s) 235, 271, 327
LmnI GCTCC 1 cut(s) 102
LpnPI CCDG 9 cut(s) 30, 346, 373, 442, 484, 554, 654, 667, 676
Lsp1109I GCAGC 2 cut(s) 66, 317
MaeII ACGT 1 cut(s) 419
MaeIII GTNAC 4 cut(s) 31, 80, 101, 196
MalI GATC 3 cut(s) 237, 273, 329
MbiI CCGCTC 1 cut(s) 141
MboI GATC 3 cut(s) 235, 271, 327
MboII GAAGA 3 cut(s) 245, 303, 337
MflI RGATCY 2 cut(s) 235, 271
MluCI AATT 1 cut(s) 44
MseI TTAA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 3 cut(s) 429, 471, 654
MspR9I CCNGG 2 cut(s) 361, 472
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 361
MwoI GCNNNNNNNGC 6 cut(s) 60, 63, 314, 407, 541, 630
NciI CCSGG 1 cut(s) 472
NdeII GATC 3 cut(s) 235, 271, 327
NlaIII CATG 2 cut(s) 496, 614
NlaIV GGNNCC 4 cut(s) 10, 475, 561, 588
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 294
PctI GAATGC 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 653
PkrI GCNGC 7 cut(s) 56, 65, 113, 307, 409, 466, 537
Psp6I CCWGG 1 cut(s) 359
PspEI GGTNACC 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 540
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 4 cut(s) 10, 475, 561, 588
PspPI GGNCC 3 cut(s) 8, 188, 474
PsrI GAACNNNNNNTAC 2 cut(s) 117, 149
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 2 cut(s) 235, 271
PsyI GACNNNGTC 1 cut(s) 421
RsaI GTAC 2 cut(s) 126, 649
RsaNI GTAC 2 cut(s) 125, 648
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 7 cut(s) 55, 64, 112, 306, 408, 465, 536
Sau3AI GATC 3 cut(s) 235, 271, 327
Sau96I GGNCC 3 cut(s) 8, 188, 474
ScaI AGTACT 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 361, 472
SfcI CTRYAG 1 cut(s) 52
SinI GGWCC 2 cut(s) 8, 474
Sse9I AATT 1 cut(s) 44
SspI AATATT 1 cut(s) 604
StyD4I CCNGG 2 cut(s) 359, 470
StyI CCWWGG 4 cut(s) 27, 106, 582, 619
TaaI ACNGT 3 cut(s) 518, 559, 596
TaiI ACGT 1 cut(s) 422
TaqI TCGA 2 cut(s) 240, 288
TasI AATT 1 cut(s) 44
TatI WGTACW 2 cut(s) 124, 647
TauI GCSGC 5 cut(s) 66, 114, 410, 467, 538
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 3 cut(s) 466, 599, 695
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 54, 305
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 338, 369
TspGWI ACGGA 4 cut(s) 89, 184, 515, 642
TspRI CASTG 3 cut(s) 466, 599, 695
Tth111I GACNNNGTC 1 cut(s) 421
VpaK11BI GGWCC 2 cut(s) 8, 474
XagI CCTNNNNNAGG 1 cut(s) 26
XapI RAATTY 1 cut(s) 44
ZraI GACGTC 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.