MD16G1102100.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005433
Physical Location & Seq
Reverse (-)
7126279 .. 7127215
937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1102100.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCCGAACCCTCGCCTGACCTCACCAAGGTCATCAACATCAAGTTACTCTGCAGCCACGGCGGCAAAATCCTCCCCCGTTACCCCGACGGCAAGCTCCGTTACCTCGGCGGGGAAACCCGTGTCCTTGCCGTTCCCCGCTCTATTTCCTTTTCCGAACTGTCGTCCAAGCTGACTGAGTTGTGTGGGCCATCCGTGACGGCCGTCAGTCTGCGCTGTCAACTGCCCACCGAGGATCTCGACGCCCTCGTATCCATCAAGTCCGATGAGGATCTCGTTAATCTCATTGAGGAATACGACAGAGCAGCCTCTTCCGCTAATCTGAAGATCAGAGCCTTCCTCTCACTCATCCCCACCGGCAGGACACCCAAAAGCATTTCATCCCCTTCTTCCTCCTCGGCCTCTGCCGCCTCCACGACTTCGTCCTCCGGCACCTCCTCGTCGTCAAATAACTACTTCGGCGCCGCTTCCGGTTCCACCCCAAGATTCCCCTTGCCGTCTTCCACCCTAGTTGATCGTTGCCTTCGTCAAATGCCGCTGCAGCCATCCCCGTCGACGGTGCCGTTTCAGAGACCCCCCTCCAAGGTTCCTCACTGTGCTAATATTTACCATGCTCGTCACGGCCACAGAAACAACAACCCTACTGGTACTACCGGTAGTCGTCTCTACCTGGTTCACAACGGCAATCACTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

233

Amino Acids

24.88

Weight (kDa)

9.45

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 30 - 109 1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 431, 461, 559
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 554
AciI CCGC 7 cut(s) 63, 111, 139, 315, 408, 465, 536
AclWI GGATC 2 cut(s) 243, 279
AcoI YGGCCR 3 cut(s) 3, 201, 622
AcuI CTGAAG 1 cut(s) 344
AcyI GRCGYC 2 cut(s) 243, 462
AfaI GTAC 1 cut(s) 649
AfiI CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
AgeI ACCGGT 1 cut(s) 653
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 2 cut(s) 97, 172
AluI AGCT 2 cut(s) 97, 172
Alw26I GTCTC 2 cut(s) 565, 668
AlwI GGATC 2 cut(s) 243, 279
AoxI GGCC 5 cut(s) 3, 188, 201, 399, 622
ApeKI GCWGC 4 cut(s) 54, 305, 538, 541
AsiGI ACCGGT 1 cut(s) 653
AspLEI GCGC 2 cut(s) 216, 464
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 16
BanI GGYRCC 3 cut(s) 431, 461, 559
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 4 cut(s) 66, 317, 525, 553
BccI CCATC 3 cut(s) 199, 263, 553
BceAI ACGGC 9 cut(s) 76, 106, 116, 188, 216, 481, 547, 637, 697
BciT130I CCWGG 1 cut(s) 671
BciVI GTATCC 1 cut(s) 262
BcoDI GTCTC 2 cut(s) 565, 668
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 2 cut(s) 52, 539
BfoI RGCGCY 1 cut(s) 465
BfuI GTATCC 1 cut(s) 262
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 8 cut(s) 55, 64, 306, 408, 465, 536, 539, 542
BlsI GCNGC 8 cut(s) 56, 65, 307, 409, 466, 537, 540, 543
Bme1390I CCNGG 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 5 cut(s) 433, 463, 475, 561, 588
BmrFI CCNGG 1 cut(s) 671
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 492
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 2 cut(s) 243, 462
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 6 cut(s) 27, 58, 106, 231, 396, 582
BsaWI WCCGGW 2 cut(s) 470, 653
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
Bse118I RCCGGY 2 cut(s) 356, 653
Bse1I ACTGG 2 cut(s) 649, 695
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 6 cut(s) 27, 58, 106, 231, 396, 582
BseGI GGATG 4 cut(s) 191, 348, 380, 545
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 649, 695
BseRI GAGGAG 2 cut(s) 385, 427
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 4 cut(s) 66, 317, 525, 553
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 5 cut(s) 5, 190, 203, 401, 624
BshNI GGYRCC 3 cut(s) 431, 461, 559
BshTI ACCGGT 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 4 cut(s) 357, 429, 471, 654
BslI CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
BsmAI GTCTC 2 cut(s) 565, 668
BsmBI CGTCTC 1 cut(s) 668
BsnI GGCC 5 cut(s) 5, 190, 203, 401, 624
Bso31I GGTCTC 1 cut(s) 565
Bsp143I GATC 4 cut(s) 235, 271, 327, 514
BspACI CCGC 7 cut(s) 63, 111, 139, 315, 408, 465, 536
BspANI GGCC 5 cut(s) 5, 190, 203, 401, 624
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 5 cut(s) 433, 463, 475, 561, 588
BspMAI CTGCAG 2 cut(s) 56, 543
BspPI GGATC 2 cut(s) 243, 279
BspT107I GGYRCC 3 cut(s) 431, 461, 559
BspTNI GGTCTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 141
BsrFI RCCGGY 2 cut(s) 356, 653
BsrI ACTGG 2 cut(s) 649, 695
BssAI RCCGGY 2 cut(s) 356, 653
BssECI CCNNGG 6 cut(s) 27, 58, 106, 231, 396, 582
BssMI GATC 4 cut(s) 235, 271, 327, 514
BssNI GRCGYC 2 cut(s) 243, 462
BssT1I CCWWGG 2 cut(s) 27, 582
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 3 cut(s) 162, 559, 596
Bst6I CTCTTC 1 cut(s) 316
BstACI GRCGYC 2 cut(s) 243, 462
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 177
BstDSI CCRYGG 1 cut(s) 58
BstENI CCTNNNNNAGG 1 cut(s) 26
BstF5I GGATG 4 cut(s) 191, 348, 380, 545
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 2 cut(s) 216, 464
BstKTI GATC 4 cut(s) 238, 274, 330, 517
BstMAI GTCTC 2 cut(s) 565, 668
BstMBI GATC 4 cut(s) 235, 271, 327, 514
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 5 cut(s) 60, 63, 314, 407, 541
BstNI CCWGG 1 cut(s) 671
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 669
BstSFI CTRYAG 2 cut(s) 52, 539
BstV1I GCAGC 4 cut(s) 66, 317, 525, 553
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 2 cut(s) 235, 271
BstYI RGATCY 2 cut(s) 235, 271
BstZI CGGCCG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 5 cut(s) 5, 190, 203, 401, 624
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 4 cut(s) 191, 348, 380, 545
BtsIMutI CAGTG 2 cut(s) 592, 688
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 2 cut(s) 216, 464
Cfr10I RCCGGY 2 cut(s) 356, 653
Cfr13I GGNCC 1 cut(s) 188
CseI GACGC 1 cut(s) 251
CsiI ACCWGGT 1 cut(s) 669
Csp6I GTAC 1 cut(s) 648
CspAI ACCGGT 1 cut(s) 653
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CviAII CATG 1 cut(s) 611
CviQI GTAC 1 cut(s) 648
DdeI CTNAG 1 cut(s) 177
DinI GGCGCC 1 cut(s) 463
DpnI GATC 4 cut(s) 237, 273, 329, 516
DpnII GATC 4 cut(s) 235, 271, 327, 514
EaeI YGGCCR 3 cut(s) 3, 201, 622
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 27, 582
Eco31I GGTCTC 1 cut(s) 565
Eco52I CGGCCG 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 344
EcoNI CCTNNNNNAGG 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 27, 582
EgeI GGCGCC 1 cut(s) 463
EheI GGCGCC 1 cut(s) 463
ErhI CCWWGG 2 cut(s) 27, 582
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 1 cut(s) 614
FaiI YATR 1 cut(s) 612
FatI CATG 1 cut(s) 610
FauI CCCGC 2 cut(s) 104, 146
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 8 cut(s) 55, 64, 306, 408, 465, 536, 539, 542
FokI GGATG 4 cut(s) 178, 335, 367, 532
Fsp4HI GCNGC 8 cut(s) 55, 64, 306, 408, 465, 536, 539, 542
FspBI CTAG 1 cut(s) 509
GlaI GCGC 2 cut(s) 215, 463
GluI GCNGC 8 cut(s) 55, 64, 306, 408, 465, 536, 539, 542
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 5 cut(s) 5, 190, 203, 401, 624
HapII CCGG 4 cut(s) 357, 429, 471, 654
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 216, 464
Hin1I GRCGYC 2 cut(s) 243, 462
Hin1II CATG 1 cut(s) 614
Hin6I GCGC 2 cut(s) 214, 462
HinP1I GCGC 2 cut(s) 214, 462
HincII GTYRAC 2 cut(s) 221, 555
HindII GTYRAC 2 cut(s) 221, 555
HinfI GANTC 1 cut(s) 486
HpaII CCGG 4 cut(s) 357, 429, 471, 654
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 221, 555, 676
Hpy188I TCNGA 5 cut(s) 157, 265, 324, 332, 570
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 3 cut(s) 221, 555, 676
Hpy99I CGWCG 5 cut(s) 92, 245, 445, 556, 559
HpyAV CCTTC 3 cut(s) 346, 396, 533
HpyCH4III ACNGT 3 cut(s) 162, 559, 596
HpyCH4V TGCA 2 cut(s) 54, 541
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 63, 314, 407, 541
HpyF3I CTNAG 1 cut(s) 177
Hsp92I GRCGYC 2 cut(s) 243, 462
Hsp92II CATG 1 cut(s) 614
HspAI GCGC 2 cut(s) 214, 462
KasI GGCGCC 1 cut(s) 461
Kzo9I GATC 4 cut(s) 235, 271, 327, 514
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 4 cut(s) 66, 317, 525, 553
MabI ACCWGGT 1 cut(s) 669
MaeI CTAG 1 cut(s) 509
MaeIII GTNAC 5 cut(s) 45, 80, 101, 196, 617
MalI GATC 4 cut(s) 237, 273, 329, 516
MbiI CCGCTC 1 cut(s) 141
MboI GATC 4 cut(s) 235, 271, 327, 514
MboII GAAGA 4 cut(s) 303, 337, 381, 492
MflI RGATCY 2 cut(s) 235, 271
Mly113I GGCGCC 1 cut(s) 462
MseI TTAA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 4 cut(s) 357, 429, 471, 654
MspR9I CCNGG 1 cut(s) 671
MvaI CCWGG 1 cut(s) 671
MwoI GCNNNNNNNGC 5 cut(s) 60, 63, 314, 407, 541
NarI GGCGCC 1 cut(s) 462
NdeII GATC 4 cut(s) 235, 271, 327, 514
NlaIII CATG 1 cut(s) 614
NlaIV GGNNCC 5 cut(s) 433, 463, 475, 561, 588
NmeAIII GCCGAG 2 cut(s) 87, 377
NmuCI GTSAC 2 cut(s) 196, 617
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 523, 560
PfeI GAWTC 1 cut(s) 486
PflFI GACNNNGTC 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 653
PkrI GCNGC 8 cut(s) 56, 65, 307, 409, 466, 537, 540, 543
PluTI GGCGCC 1 cut(s) 465
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 669
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 5 cut(s) 433, 463, 475, 561, 588
PspPI GGNCC 1 cut(s) 188
PstI CTGCAG 2 cut(s) 56, 543
PsuI RGATCY 2 cut(s) 235, 271
PsyI GACNNNGTC 1 cut(s) 421
RsaI GTAC 1 cut(s) 649
RsaNI GTAC 1 cut(s) 648
SalI GTCGAC 1 cut(s) 553
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 8 cut(s) 55, 64, 306, 408, 465, 536, 539, 542
Sau3AI GATC 4 cut(s) 235, 271, 327, 514
Sau96I GGNCC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 671
SetI ASST 8 cut(s) 24, 33, 99, 108, 174, 437, 588, 672
SexAI ACCWGGT 1 cut(s) 669
SfcI CTRYAG 2 cut(s) 52, 539
SfoI GGCGCC 1 cut(s) 463
SgrDI CGTCGACG 1 cut(s) 553
SsiI CCGC 7 cut(s) 63, 111, 139, 315, 408, 465, 536
SspDI GGCGCC 1 cut(s) 461
SspI AATATT 1 cut(s) 604
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 669
StyI CCWWGG 2 cut(s) 27, 582
TaaI ACNGT 3 cut(s) 162, 559, 596
TaqI TCGA 2 cut(s) 240, 554
TauI GCSGC 4 cut(s) 66, 410, 467, 538
TfiI GAWTC 1 cut(s) 486
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 599, 695
TseFI GTSAC 2 cut(s) 196, 617
TseI GCWGC 4 cut(s) 54, 305, 538, 541
Tsp45I GTSAC 2 cut(s) 196, 617
TspDTI ATGAA 1 cut(s) 369
TspGWI ACGGA 2 cut(s) 89, 184
TspRI CASTG 2 cut(s) 599, 695
Tth111I GACNNNGTC 1 cut(s) 421
XagI CCTNNNNNAGG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.