FvH4_7g20480

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005433
Physical Location & Seq
Reverse (-)
16661676 .. 16662958
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20480.t1

Sequence Viewer

Length: 678 bp
ATGATGTCCTTCATCAAAATCCCCAAGTCCTCTCTCTCTAACTCAAACCCTAAAGTTGAAGCCACCATGGTCAGACCTTCAGCTCACCCCACCAACCACACTCTCAAATTCCTCTACAGCTACGGCGGCAAGATCCTCCCTCGTCACCCCGACGGCAAGCTCCGTTATCACGGCGGAACAACCCGCGTCCTCGCCGTGCCCCGCTCCATCTCCTTCCCCAAGCTCTTGTTAAAGCTAGAAGAGCTCTGTGGCGCAACCGTCGTTCTCCGTTGTCAACTCCCAACCGACGACCTCGACACCCTCATCTCCATCAAGAACGACGAGGACCTCGCCAATATCGTCGAGGAGTACGACACAGCCGCCACGCCGTTGAAGATCAGAGCCATCCTTTCGCTCCTCCCCGCCAAGAAACCTCCGTCATCTTCGACGTCGTCCTCGAAGTCTTCATCTTCAAATCACAGTGGTGGTACTACATGTGCCTCCGGATCCTCCATGCCGTTCCCGAAGGAGCGTTGCCCTTGTCCCCGTCCAGTTTCTCATACGGTGGCGTTTCAGCGAGCTCCAGCAGCAGTTTGCAAAGCTCATCTTCACTGTGCTAAGTACCATGCTCATGCAAACCCTAGCCGTCTCTGTCACGTCAACAACAATAACACACCATGTCCATATGCATGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

226

Amino Acids

24.46

Weight (kDa)

9.54

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 51 - 121 2e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 431
AccBSI CCGCTC 1 cut(s) 204
AccII CGCG 1 cut(s) 186
AccIII TCCGGA 1 cut(s) 482
AciI CCGC 6 cut(s) 126, 174, 184, 202, 360, 402
AclWI GGATC 3 cut(s) 127, 480, 493
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 63
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 3 cut(s) 350, 469, 602
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 3 cut(s) 59, 373, 453
AjiI CACGTC 1 cut(s) 637
AleI CACNNNNGTG 1 cut(s) 462
AluBI AGCT 8 cut(s) 83, 120, 160, 223, 235, 244, 560, 581
AluI AGCT 8 cut(s) 83, 120, 160, 223, 235, 244, 560, 581
Alw21I GWGCWC 2 cut(s) 246, 562
Alw26I GTCTC 1 cut(s) 632
AlwI GGATC 3 cut(s) 127, 480, 493
Aor13HI TCCGGA 1 cut(s) 482
ApeKI GCWGC 1 cut(s) 566
ApoI RAATTY 1 cut(s) 107
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 77, 137
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 201
BamHI GGATCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 246, 562
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 246, 562
BbvI GCAGC 1 cut(s) 578
BccI CCATC 3 cut(s) 215, 317, 392
BceAI ACGGC 7 cut(s) 139, 169, 179, 187, 352, 481, 609
BcoDI GTCTC 1 cut(s) 632
BfaI CTAG 2 cut(s) 236, 621
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 3 cut(s) 127, 360, 567
BlsI GCNGC 3 cut(s) 128, 361, 568
Bme18I GGWCC 1 cut(s) 325
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 482
Bse1I ACTGG 1 cut(s) 530
BseAI TCCGGA 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 1 cut(s) 384
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 2 cut(s) 359, 386
BseSI GKGCMC 1 cut(s) 201
BseXI GCAGC 1 cut(s) 578
Bsh1236I CGCG 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 246, 562
BsiSI CCGG 1 cut(s) 483
BslFI GGGAC 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 632
BsmBI CGTCTC 1 cut(s) 632
BsmFI GGGAC 1 cut(s) 507
Bsp1286I GDGCHC 3 cut(s) 201, 246, 562
Bsp13I TCCGGA 1 cut(s) 482
Bsp143I GATC 3 cut(s) 132, 375, 485
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 6 cut(s) 126, 174, 184, 202, 360, 402
BspEI TCCGGA 1 cut(s) 482
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 487
BspPI GGATC 3 cut(s) 127, 480, 493
BspQI GCTCTTC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 3 cut(s) 132, 375, 485
BssNI GRCGYC 1 cut(s) 428
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 4 cut(s) 259, 461, 544, 593
Bst6I CTCTTC 1 cut(s) 234
BstACI GRCGYC 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 158, 558
BstDEI CTNAG 1 cut(s) 597
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 384
BstFNI CGCG 1 cut(s) 186
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 3 cut(s) 135, 378, 488
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 3 cut(s) 132, 375, 485
BstMWI GCNNNNNNNGC 3 cut(s) 126, 241, 566
BstNSI RCATGY 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 115
BstSLI GKGCMC 1 cut(s) 201
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 578
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 2 cut(s) 132, 485
BstXI CCANNNNNNTGG 1 cut(s) 669
BstYI RGATCY 2 cut(s) 132, 485
BtgI CCRYGG 1 cut(s) 66
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 2 cut(s) 466, 589
Cac8I GCNNGC 2 cut(s) 158, 558
CfoI GCGC 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 325
CseI GACGC 1 cut(s) 175
Csp6I GTAC 3 cut(s) 349, 468, 601
CviAII CATG 7 cut(s) 67, 474, 493, 605, 611, 657, 669
CviQI GTAC 3 cut(s) 349, 468, 601
DdeI CTNAG 1 cut(s) 597
DpnI GATC 3 cut(s) 134, 377, 487
DpnII GATC 3 cut(s) 132, 375, 485
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EciI GGCGGA 1 cut(s) 189
Ecl136II GAGCTC 2 cut(s) 244, 560
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 246, 562
Eco47I GGWCC 1 cut(s) 325
Eco53kI GAGCTC 2 cut(s) 244, 560
Eco57I CTGAAG 1 cut(s) 63
EcoICRI GAGCTC 2 cut(s) 244, 560
EcoO109I RGGNCCY 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 2 cut(s) 246, 562
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 632
FaeI CATG 7 cut(s) 70, 477, 496, 608, 614, 660, 672
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 507
FatI CATG 7 cut(s) 66, 473, 492, 604, 610, 656, 668
FauI CCCGC 3 cut(s) 191, 209, 409
FauNDI CATATG 1 cut(s) 664
Fnu4HI GCNGC 3 cut(s) 127, 360, 567
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 2 cut(s) 246, 562
Fsp4HI GCNGC 3 cut(s) 127, 360, 567
FspBI CTAG 2 cut(s) 236, 621
GlaI GCGC 1 cut(s) 253
GluI GCNGC 3 cut(s) 127, 360, 567
GsuI CTGGAG 1 cut(s) 546
HapII CCGG 1 cut(s) 483
HgaI GACGC 1 cut(s) 175
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 7 cut(s) 70, 477, 496, 608, 614, 660, 672
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HincII GTYRAC 2 cut(s) 275, 640
HindII GTYRAC 2 cut(s) 275, 640
HpaII CCGG 1 cut(s) 483
HphI GGTGA 2 cut(s) 77, 137
Hpy166II GTNNAC 2 cut(s) 275, 640
Hpy188I TCNGA 2 cut(s) 74, 380
Hpy188III TCNNGA 3 cut(s) 313, 483, 502
Hpy8I GTNNAC 2 cut(s) 275, 640
Hpy99I CGWCG 7 cut(s) 155, 263, 290, 323, 344, 430, 433
HpyAV CCTTC 4 cut(s) 19, 87, 223, 499
HpyCH4III ACNGT 4 cut(s) 259, 461, 544, 593
HpyCH4IV ACGT 2 cut(s) 428, 636
HpyCH4V TGCA 3 cut(s) 576, 614, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 241, 566
HpyF3I CTNAG 1 cut(s) 597
HpySE526I ACGT 2 cut(s) 428, 636
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 7 cut(s) 70, 477, 496, 608, 614, 660, 672
HspAI GCGC 1 cut(s) 252
Kpn2I TCCGGA 1 cut(s) 482
Kzo9I GATC 3 cut(s) 132, 375, 485
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 5 cut(s) 165, 209, 399, 508, 565
LpnPI CCDG 3 cut(s) 496, 543, 576
Lsp1109I GCAGC 1 cut(s) 578
MaeI CTAG 2 cut(s) 236, 621
MaeII ACGT 2 cut(s) 428, 636
MaeIII GTNAC 2 cut(s) 143, 632
MalI GATC 3 cut(s) 134, 377, 487
MbiI CCGCTC 1 cut(s) 204
MboI GATC 3 cut(s) 132, 375, 485
MboII GAAGA 6 cut(s) 251, 385, 414, 435, 441, 578
MflI RGATCY 2 cut(s) 132, 485
MhlI GDGCHC 3 cut(s) 201, 246, 562
MluCI AATT 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 670
MroI TCCGGA 1 cut(s) 482
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 3 cut(s) 462, 609, 667
MspI CCGG 1 cut(s) 483
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 126, 241, 566
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 664
NdeII GATC 3 cut(s) 132, 375, 485
NlaIII CATG 7 cut(s) 70, 477, 496, 608, 614, 660, 672
NlaIV GGNNCC 1 cut(s) 487
NmuCI GTSAC 2 cut(s) 143, 632
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 477
OliI CACNNNNGTG 1 cut(s) 462
PciI ACATGT 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 3 cut(s) 336, 348, 434
PflFI GACNNNGTC 1 cut(s) 430
PkrI GCNGC 3 cut(s) 128, 361, 568
PpuMI RGGWCCY 1 cut(s) 325
PscI ACATGT 1 cut(s) 473
Psp124BI GAGCTC 2 cut(s) 246, 562
Psp5II RGGWCCY 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 1 cut(s) 325
PspPPI RGGWCCY 1 cut(s) 325
PsuI RGATCY 2 cut(s) 132, 485
PsyI GACNNNGTC 1 cut(s) 430
RsaI GTAC 3 cut(s) 350, 469, 602
RsaNI GTAC 3 cut(s) 349, 468, 601
RseI CAYNNNNRTG 3 cut(s) 462, 609, 667
SacI GAGCTC 2 cut(s) 246, 562
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 3 cut(s) 127, 360, 567
Sau3AI GATC 3 cut(s) 132, 375, 485
Sau96I GGNCC 1 cut(s) 325
SduI GDGCHC 3 cut(s) 201, 246, 562
SfcI CTRYAG 1 cut(s) 115
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 3 cut(s) 462, 609, 667
Sse9I AATT 1 cut(s) 107
SsiI CCGC 6 cut(s) 126, 174, 184, 202, 360, 402
SspMI CTAG 2 cut(s) 236, 621
SstI GAGCTC 2 cut(s) 246, 562
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 4 cut(s) 259, 461, 544, 593
TaiI ACGT 2 cut(s) 431, 639
TaqI TCGA 4 cut(s) 294, 342, 425, 437
TasI AATT 1 cut(s) 107
TauI GCSGC 2 cut(s) 129, 362
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 2 cut(s) 466, 596
TseFI GTSAC 2 cut(s) 143, 632
TseI GCWGC 1 cut(s) 566
Tsp45I GTSAC 2 cut(s) 143, 632
TspDTI ATGAA 1 cut(s) 435
TspGWI ACGGA 3 cut(s) 152, 257, 405
TspRI CASTG 2 cut(s) 466, 596
Tth111I GACNNNGTC 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 1 cut(s) 107
XceI RCATGY 1 cut(s) 477
XspI CTAG 2 cut(s) 236, 621
ZraI GACGTC 1 cut(s) 429
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.