Rw4G027700

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005433
Physical Location & Seq
Forward (+)
54282734 .. 54283432
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027700.1

Sequence Viewer

Length: 609 bp
ATGGTCGGACCATCAGCTGACCCCAACAACACTCTCAAATTCCTCTGCAGCTACGGCGGCAAAATCCTCCCCCGTTACCCTGACGGCAAGCTCCGTTATCTCGGCGGCGAAACCCGCGTCCTTGCCGTCCACCGTTCCATTTCCTTCTCTGAGCTATTGCTGAAGCTGGGAGAGCTGTGTGGCACAACCGTCGGTCTCCGCTGCCAACTGCCAACCGAAGACCTCGACGCCCTTATCTCCGTCAAGTGCGACGAGGACCTCGCCAATCTCATCGAGGAGTACGACACAGCTGCGGCGGCCACCACGCCGTTGAAGATCAGAGCTTTCCTTTCGCTCCCCGCCAACAAAATTCCGACGTCTCCTCAGTCATCCTCGGCGTCCTCCTCGAAATCCTCGTCTTCAAATCACAGTACTACATATGCCTCCGGATCCACTCCCAGATTCTCGATGCCGTTGCCGGCGGAACGTTGCCTCCGTCAAGTTTCGCATACGGCGCCATTTCAGCGAGCTCCAGCAGCAGCTGGGAAAGCTCCTCACTGTGCTAATTACCAAGCTCATGGAAACCCTAGCCGTCTCTACCTCGTTCACAACGGCAACCACTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

202

Amino Acids

21.71

Weight (kDa)

8.93

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 28 - 97 5.1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 493
AccII CGCG 1 cut(s) 117
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 8 cut(s) 57, 105, 115, 199, 293, 296, 339, 461
AclI AACGTT 1 cut(s) 466
AclWI GGATC 2 cut(s) 423, 436
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 2 cut(s) 38, 348
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 4 cut(s) 228, 356, 377, 494
AfaI GTAC 2 cut(s) 281, 412
AgsI TTSAA 2 cut(s) 313, 402
AloI GAACNNNNNNTCC 2 cut(s) 456, 488
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 3 cut(s) 200, 363, 578
AlwI GGATC 2 cut(s) 423, 436
AlwNI CAGNNNCTG 1 cut(s) 521
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 5 cut(s) 48, 201, 290, 515, 518
ApoI RAATTY 2 cut(s) 38, 348
ArsI GACNNNNNNTTYG 4 cut(s) 102, 134, 380, 412
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 2 cut(s) 8, 256
AvaII GGWCC 2 cut(s) 8, 256
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 511
BbsI GAAGAC 2 cut(s) 225, 390
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 5 cut(s) 60, 188, 277, 527, 530
BccI CCATC 1 cut(s) 19
BceAI ACGGC 8 cut(s) 70, 100, 110, 292, 436, 507, 555, 607
BcgI CGANNNNNNTGC 6 cut(s) 172, 206, 272, 306, 436, 470
BcoDI GTCTC 3 cut(s) 200, 363, 578
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 46
BfoI RGCGCY 1 cut(s) 497
BisI GCNGC 9 cut(s) 49, 58, 106, 202, 291, 294, 297, 516, 519
BlsI GCNGC 9 cut(s) 50, 59, 107, 203, 292, 295, 298, 517, 520
BmcAI AGTACT 1 cut(s) 412
Bme18I GGWCC 2 cut(s) 8, 256
BmgT120I GGNCC 2 cut(s) 8, 256
BmiI GGNNCC 2 cut(s) 430, 495
BmsI GCATC 1 cut(s) 438
BpiI GAAGAC 2 cut(s) 225, 390
BpmI CTGGAG 1 cut(s) 495
BsaHI GRCGYC 4 cut(s) 228, 356, 377, 494
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 372
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bse118I RCCGGY 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 605
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 368
BseMII CTCAG 2 cut(s) 141, 377
BseNI ACTGG 1 cut(s) 605
BseRI GAGGAG 4 cut(s) 290, 351, 373, 522
BseXI GCAGC 5 cut(s) 60, 188, 277, 527, 530
BseYI CCCAGC 2 cut(s) 166, 521
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 2 cut(s) 426, 458
BsmAI GTCTC 3 cut(s) 200, 363, 578
BsmBI CGTCTC 2 cut(s) 363, 578
BsnI GGCC 1 cut(s) 299
Bso31I GGTCTC 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 511
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 2 cut(s) 315, 428
BspACI CCGC 8 cut(s) 57, 105, 115, 199, 293, 296, 339, 461
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 2 cut(s) 142, 376
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 430, 495
BspMAI CTGCAG 1 cut(s) 50
BspPI GGATC 2 cut(s) 423, 436
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 457
BsrI ACTGG 1 cut(s) 605
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 2 cut(s) 315, 428
BssNI GRCGYC 4 cut(s) 228, 356, 377, 494
Bst4CI ACNGT 4 cut(s) 134, 190, 410, 539
BstACI GRCGYC 4 cut(s) 228, 356, 377, 494
BstC8I GCNNGC 3 cut(s) 89, 459, 507
BstDEI CTNAG 2 cut(s) 150, 363
BstF5I GGATG 1 cut(s) 368
BstFNI CGCG 1 cut(s) 117
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 2 cut(s) 318, 431
BstMAI GTCTC 3 cut(s) 200, 363, 578
BstMBI GATC 2 cut(s) 315, 428
BstMWI GCNNNNNNNGC 9 cut(s) 54, 57, 114, 172, 296, 493, 502, 515, 527
BstSFI CTRYAG 1 cut(s) 46
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 5 cut(s) 60, 188, 277, 527, 530
BstV2I GAAGAC 2 cut(s) 225, 390
BstX2I RGATCY 1 cut(s) 428
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 1 cut(s) 299
BtsCI GGATG 1 cut(s) 368
BtsIMutI CAGTG 2 cut(s) 535, 598
Cac8I GCNNGC 3 cut(s) 89, 459, 507
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 496
Cfr10I RCCGGY 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 8, 256
CseI GACGC 3 cut(s) 106, 236, 366
Csp6I GTAC 2 cut(s) 280, 411
CviAII CATG 1 cut(s) 557
CviQI GTAC 2 cut(s) 280, 411
DdeI CTNAG 2 cut(s) 150, 363
DinI GGCGCC 1 cut(s) 495
DpnI GATC 2 cut(s) 317, 430
DpnII GATC 2 cut(s) 315, 428
EaeI YGGCCR 1 cut(s) 297
EciI GGCGGA 1 cut(s) 476
Ecl136II GAGCTC 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 2 cut(s) 8, 256
Eco53kI GAGCTC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 511
EgeI GGCGCC 1 cut(s) 495
EheI GGCGCC 1 cut(s) 495
Esp3I CGTCTC 2 cut(s) 363, 578
FaeI CATG 1 cut(s) 560
FaiI YATR 4 cut(s) 418, 420, 489, 558
FatI CATG 1 cut(s) 556
FauI CCCGC 2 cut(s) 122, 346
FauNDI CATATG 1 cut(s) 418
Fnu4HI GCNGC 9 cut(s) 49, 58, 106, 202, 291, 294, 297, 516, 519
FokI GGATG 1 cut(s) 355
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 9 cut(s) 49, 58, 106, 202, 291, 294, 297, 516, 519
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 495
GluI GCNGC 9 cut(s) 49, 58, 106, 202, 291, 294, 297, 516, 519
GsaI CCCAGC 2 cut(s) 170, 525
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 1 cut(s) 299
HapII CCGG 2 cut(s) 426, 458
HgaI GACGC 3 cut(s) 106, 236, 366
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 4 cut(s) 228, 356, 377, 494
Hin1II CATG 1 cut(s) 560
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HinfI GANTC 1 cut(s) 441
HpaII CCGG 2 cut(s) 426, 458
Hpy166II GTNNAC 2 cut(s) 130, 586
Hpy188I TCNGA 4 cut(s) 8, 151, 320, 354
Hpy188III TCNNGA 2 cut(s) 426, 445
Hpy8I GTNNAC 2 cut(s) 130, 586
Hpy99I CGWCG 4 cut(s) 194, 230, 254, 358
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 4 cut(s) 134, 190, 410, 539
HpyCH4IV ACGT 2 cut(s) 356, 466
HpyCH4V TGCA 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 9 cut(s) 54, 57, 114, 172, 296, 493, 502, 515, 527
HpyF3I CTNAG 2 cut(s) 150, 363
HpySE526I ACGT 2 cut(s) 356, 466
Hsp92I GRCGYC 4 cut(s) 228, 356, 377, 494
Hsp92II CATG 1 cut(s) 560
HspAI GCGC 1 cut(s) 494
KasI GGCGCC 1 cut(s) 493
Kpn2I TCCGGA 1 cut(s) 425
KroI GCCGGC 1 cut(s) 457
KroNI GCCGGC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 315, 428
LmnI GCTCC 4 cut(s) 96, 339, 514, 535
LpnPI CCDG 8 cut(s) 93, 152, 439, 451, 471, 507, 525, 586
Lsp1109I GCAGC 5 cut(s) 60, 188, 277, 527, 530
LweI GCATC 1 cut(s) 438
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 356, 466
MaeIII GTNAC 1 cut(s) 74
MalI GATC 2 cut(s) 317, 430
MboI GATC 2 cut(s) 315, 428
MboII GAAGA 3 cut(s) 230, 325, 390
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 1 cut(s) 511
MluCI AATT 3 cut(s) 38, 348, 544
Mly113I GGCGCC 1 cut(s) 494
MmeI TCCRAC 1 cut(s) 377
MroI TCCGGA 1 cut(s) 425
MroNI GCCGGC 1 cut(s) 457
MspA1I CMGCKG 4 cut(s) 17, 201, 290, 521
MspI CCGG 2 cut(s) 426, 458
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 9 cut(s) 54, 57, 114, 172, 296, 493, 502, 515, 527
NaeI GCCGGC 1 cut(s) 459
NarI GGCGCC 1 cut(s) 494
NdeI CATATG 1 cut(s) 418
NdeII GATC 2 cut(s) 315, 428
NgoMIV GCCGGC 1 cut(s) 457
NlaIII CATG 1 cut(s) 560
NlaIV GGNNCC 2 cut(s) 430, 495
NmeAIII GCCGAG 2 cut(s) 81, 353
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 392, 472
PdiI GCCGGC 1 cut(s) 459
PfeI GAWTC 1 cut(s) 441
PkrI GCNGC 9 cut(s) 50, 59, 107, 203, 292, 295, 298, 517, 520
PluTI GGCGCC 1 cut(s) 497
PpuMI RGGWCCY 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 511
Psp1406I AACGTT 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 256
PspFI CCCAGC 2 cut(s) 166, 521
PspN4I GGNNCC 2 cut(s) 430, 495
PspPI GGNCC 2 cut(s) 8, 256
PspPPI RGGWCCY 1 cut(s) 256
PstI CTGCAG 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 428
PvuII CAGCTG 3 cut(s) 17, 290, 521
RsaI GTAC 2 cut(s) 281, 412
RsaNI GTAC 2 cut(s) 280, 411
SacI GAGCTC 1 cut(s) 511
SatI GCNGC 9 cut(s) 49, 58, 106, 202, 291, 294, 297, 516, 519
Sau3AI GATC 2 cut(s) 315, 428
Sau96I GGNCC 2 cut(s) 8, 256
ScaI AGTACT 1 cut(s) 412
SduI GDGCHC 1 cut(s) 511
SfaNI GCATC 1 cut(s) 438
SfcI CTRYAG 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 495
SinI GGWCC 2 cut(s) 8, 256
Sse9I AATT 3 cut(s) 38, 348, 544
SsiI CCGC 8 cut(s) 57, 105, 115, 199, 293, 296, 339, 461
SspDI GGCGCC 1 cut(s) 493
SspMI CTAG 1 cut(s) 567
SstI GAGCTC 1 cut(s) 511
TaaI ACNGT 4 cut(s) 134, 190, 410, 539
TaiI ACGT 2 cut(s) 359, 469
TaqI TCGA 4 cut(s) 225, 273, 386, 446
TaqII GACCGA 1 cut(s) 182
TasI AATT 3 cut(s) 38, 348, 544
TatI WGTACW 1 cut(s) 410
TauI GCSGC 4 cut(s) 60, 108, 296, 299
TfiI GAWTC 1 cut(s) 441
TscAI CASTG 2 cut(s) 542, 605
TseI GCWGC 5 cut(s) 48, 201, 290, 515, 518
TspGWI ACGGA 3 cut(s) 83, 229, 464
TspRI CASTG 2 cut(s) 542, 605
VpaK11BI GGWCC 2 cut(s) 8, 256
XapI RAATTY 2 cut(s) 38, 348
XspI CTAG 1 cut(s) 567
ZraI GACGTC 1 cut(s) 357
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.