pycom16g08640

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005433
Physical Location & Seq
Reverse (-)
5770365 .. 5771149
785 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08640.1

Sequence Viewer

Length: 699 bp
ATGGCCGGACCCTCGCCTGACCTGACCAAGGTCACCAACATCAAGTTACTCTGCAGCTACGGCGGTAAAATCCTCCCCCGTTACCCCGACGGCAAGCTCCGTTACCTCGGCGGGGAAACCCGTGTCCTTGCCGTTCCCCGCTCTATTTCCTTTTCCGAACTGTCGTCAAAGCTGACTGAGTTGTGTGGGCCATCCGTGACGGCCGTCAGTCTGCGCTGTCAACTGCCCACCGAGGATCTTGACGCCCTCGTATCCATCAAGTCCGATGAGGATCTCGTTAATCTCATTGAGGAATACGACAGAGCAGCCTCTTCCGCTAATCTGAAAATCAGAGCCTTCCTCTCACTCATCCCCGCCGGCAGGACAACCAAAAGCGTTTCATCCCCTTCTTCCTCCTCGGCCTCTGCCGCCTCCACGACTTCGTCCTCCAGCAGCTCCTCGTCGTCAAATAACTACTTCGGCGCTGCTTCCGGTTCCACCCCAAGATTCCCCTTGCCGTCTTCCACCCCAGTTGATCGTTGCCTTCGTCAAATGCCGCTGCAGCCTTCCCCGTCGACGGTGCCGTTTCAGAGATCCCCCTCCAAGGTTCCTCACTGTGCTAATATTTACCATGCTTGTCACGGCCACAGAAACAACAACCCTACTGGTACTACCGGTAGTCGTCTCTACCTGGTTCACAACGGCAATCACTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

233

Amino Acids

24.72

Weight (kDa)

9.34

Isoelectric Point (pI)

63.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 30 - 109 1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 559
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 554
AciI CCGC 7 cut(s) 63, 111, 139, 315, 354, 408, 536
AclWI GGATC 3 cut(s) 243, 279, 567
AcoI YGGCCR 3 cut(s) 3, 201, 622
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 1 cut(s) 649
AfiI CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
AgeI ACCGGT 1 cut(s) 653
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 4 cut(s) 57, 97, 172, 435
AluI AGCT 4 cut(s) 57, 97, 172, 435
Alw26I GTCTC 1 cut(s) 668
AlwI GGATC 3 cut(s) 243, 279, 567
AoxI GGCC 5 cut(s) 3, 188, 201, 399, 622
ApeKI GCWGC 6 cut(s) 54, 305, 432, 464, 538, 541
AsiGI ACCGGT 1 cut(s) 653
AspLEI GCGC 2 cut(s) 216, 464
AspS9I GGNCC 2 cut(s) 8, 188
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 559
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 6 cut(s) 66, 317, 444, 451, 525, 553
BccI CCATC 2 cut(s) 199, 263
BceAI ACGGC 9 cut(s) 76, 106, 116, 188, 216, 481, 547, 637, 697
BciT130I CCWGG 1 cut(s) 671
BciVI GTATCC 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 668
BfmI CTRYAG 2 cut(s) 52, 539
BfoI RGCGCY 1 cut(s) 465
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 8 cut(s) 55, 306, 408, 433, 465, 536, 539, 542
BlsI GCNGC 8 cut(s) 56, 307, 409, 434, 466, 537, 540, 543
Bme1390I CCNGG 1 cut(s) 671
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 8, 188
BmiI GGNNCC 4 cut(s) 10, 475, 561, 588
BmrFI CCNGG 1 cut(s) 671
BmrI ACTGGG 1 cut(s) 503
BmuI ACTGGG 1 cut(s) 503
BoxI GACNNNNGTC 2 cut(s) 29, 203
BpiI GAAGAC 1 cut(s) 492
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpmI CTGGAG 1 cut(s) 412
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 5 cut(s) 27, 106, 231, 396, 582
BsaWI WCCGGW 2 cut(s) 470, 653
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
Bse118I RCCGGY 2 cut(s) 356, 653
Bse1I ACTGG 3 cut(s) 509, 649, 695
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 5 cut(s) 27, 106, 231, 396, 582
BseGI GGATG 3 cut(s) 191, 348, 380
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 3 cut(s) 509, 649, 695
BseRI GAGGAG 2 cut(s) 385, 427
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 6 cut(s) 66, 317, 444, 451, 525, 553
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 5 cut(s) 5, 190, 203, 401, 624
BshNI GGYRCC 1 cut(s) 559
BshTI ACCGGT 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 4 cut(s) 6, 357, 471, 654
BslI CCNNNNNNNGG 5 cut(s) 28, 112, 360, 556, 583
BsmAI GTCTC 1 cut(s) 668
BsmBI CGTCTC 1 cut(s) 668
BsnI GGCC 5 cut(s) 5, 190, 203, 401, 624
Bsp143I GATC 4 cut(s) 235, 271, 514, 572
BspACI CCGC 7 cut(s) 63, 111, 139, 315, 354, 408, 536
BspANI GGCC 5 cut(s) 5, 190, 203, 401, 624
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 4 cut(s) 10, 475, 561, 588
BspMAI CTGCAG 2 cut(s) 56, 543
BspPI GGATC 3 cut(s) 243, 279, 567
BspT107I GGYRCC 1 cut(s) 559
BsrBI CCGCTC 1 cut(s) 141
BsrFI RCCGGY 2 cut(s) 356, 653
BsrI ACTGG 3 cut(s) 509, 649, 695
BssAI RCCGGY 2 cut(s) 356, 653
BssECI CCNNGG 5 cut(s) 27, 106, 231, 396, 582
BssMI GATC 4 cut(s) 235, 271, 514, 572
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 2 cut(s) 27, 582
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 3 cut(s) 162, 559, 596
Bst6I CTCTTC 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 95, 358
BstDEI CTNAG 1 cut(s) 177
BstEII GGTNACC 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 26
BstF5I GGATG 3 cut(s) 191, 348, 380
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 2 cut(s) 216, 464
BstKTI GATC 4 cut(s) 238, 274, 517, 575
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 4 cut(s) 235, 271, 514, 572
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 4 cut(s) 60, 314, 407, 541
BstNI CCWGG 1 cut(s) 671
BstPAI GACNNNNGTC 2 cut(s) 29, 203
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 669
BstSFI CTRYAG 2 cut(s) 52, 539
BstV1I GCAGC 6 cut(s) 66, 317, 444, 451, 525, 553
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 3 cut(s) 235, 271, 572
BstYI RGATCY 3 cut(s) 235, 271, 572
BstZI CGGCCG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 5 cut(s) 5, 190, 203, 401, 624
BtsCI GGATG 3 cut(s) 191, 348, 380
BtsIMutI CAGTG 2 cut(s) 592, 688
Cac8I GCNNGC 2 cut(s) 95, 358
CfoI GCGC 2 cut(s) 216, 464
Cfr10I RCCGGY 2 cut(s) 356, 653
Cfr13I GGNCC 2 cut(s) 8, 188
CseI GACGC 1 cut(s) 251
CsiI ACCWGGT 1 cut(s) 669
Csp6I GTAC 1 cut(s) 648
CspAI ACCGGT 1 cut(s) 653
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CviAII CATG 1 cut(s) 611
CviQI GTAC 1 cut(s) 648
DdeI CTNAG 1 cut(s) 177
DpnI GATC 4 cut(s) 237, 273, 516, 574
DpnII GATC 4 cut(s) 235, 271, 514, 572
EaeI YGGCCR 3 cut(s) 3, 201, 622
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 27, 582
Eco47I GGWCC 1 cut(s) 8
Eco52I CGGCCG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 31
EcoNI CCTNNNNNAGG 1 cut(s) 26
EcoO65I GGTNACC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 27, 582
ErhI CCWWGG 2 cut(s) 27, 582
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 1 cut(s) 614
FaiI YATR 1 cut(s) 612
FatI CATG 1 cut(s) 610
FauI CCCGC 3 cut(s) 104, 146, 361
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 8 cut(s) 55, 306, 408, 433, 465, 536, 539, 542
FokI GGATG 3 cut(s) 178, 335, 367
Fsp4HI GCNGC 8 cut(s) 55, 306, 408, 433, 465, 536, 539, 542
GlaI GCGC 2 cut(s) 215, 463
GluI GCNGC 8 cut(s) 55, 306, 408, 433, 465, 536, 539, 542
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 5 cut(s) 5, 190, 203, 401, 624
HapII CCGG 4 cut(s) 6, 357, 471, 654
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 216, 464
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 1 cut(s) 614
Hin6I GCGC 2 cut(s) 214, 462
HinP1I GCGC 2 cut(s) 214, 462
HincII GTYRAC 2 cut(s) 221, 555
HindII GTYRAC 2 cut(s) 221, 555
HinfI GANTC 1 cut(s) 486
HpaII CCGG 4 cut(s) 6, 357, 471, 654
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 3 cut(s) 221, 555, 676
Hpy188I TCNGA 5 cut(s) 157, 265, 324, 332, 570
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 3 cut(s) 221, 555, 676
Hpy99I CGWCG 4 cut(s) 92, 445, 556, 559
HpyAV CCTTC 4 cut(s) 346, 396, 533, 555
HpyCH4III ACNGT 3 cut(s) 162, 559, 596
HpyCH4V TGCA 2 cut(s) 54, 541
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 314, 407, 541
HpyF3I CTNAG 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 1 cut(s) 614
HspAI GCGC 2 cut(s) 214, 462
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 4 cut(s) 235, 271, 514, 572
LmnI GCTCC 2 cut(s) 102, 440
Lsp1109I GCAGC 6 cut(s) 66, 317, 444, 451, 525, 553
MabI ACCWGGT 1 cut(s) 669
MaeIII GTNAC 6 cut(s) 31, 45, 80, 101, 196, 617
MalI GATC 4 cut(s) 237, 273, 516, 574
MbiI CCGCTC 1 cut(s) 141
MboI GATC 4 cut(s) 235, 271, 514, 572
MboII GAAGA 3 cut(s) 303, 381, 492
MflI RGATCY 3 cut(s) 235, 271, 572
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 4 cut(s) 6, 357, 471, 654
MspR9I CCNGG 1 cut(s) 671
MvaI CCWGG 1 cut(s) 671
MwoI GCNNNNNNNGC 4 cut(s) 60, 314, 407, 541
NaeI GCCGGC 1 cut(s) 358
NdeII GATC 4 cut(s) 235, 271, 514, 572
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 1 cut(s) 614
NlaIV GGNNCC 4 cut(s) 10, 475, 561, 588
NmeAIII GCCGAG 2 cut(s) 87, 377
NmuCI GTSAC 3 cut(s) 31, 196, 617
PcsI WCGNNNNNNNCGW 2 cut(s) 523, 560
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 486
PflFI GACNNNGTC 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 653
PkrI GCNGC 8 cut(s) 56, 307, 409, 434, 466, 537, 540, 543
PshAI GACNNNNGTC 2 cut(s) 29, 203
Psp6I CCWGG 1 cut(s) 669
PspEI GGTNACC 1 cut(s) 31
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 4 cut(s) 10, 475, 561, 588
PspPI GGNCC 2 cut(s) 8, 188
PstI CTGCAG 2 cut(s) 56, 543
PsuI RGATCY 3 cut(s) 235, 271, 572
PsyI GACNNNGTC 1 cut(s) 421
RsaI GTAC 1 cut(s) 649
RsaNI GTAC 1 cut(s) 648
SalI GTCGAC 1 cut(s) 553
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 8 cut(s) 55, 306, 408, 433, 465, 536, 539, 542
Sau3AI GATC 4 cut(s) 235, 271, 514, 572
Sau96I GGNCC 2 cut(s) 8, 188
ScrFI CCNGG 1 cut(s) 671
SetI ASST 9 cut(s) 24, 33, 59, 99, 108, 174, 437, 588, 672
SexAI ACCWGGT 1 cut(s) 669
SfcI CTRYAG 2 cut(s) 52, 539
SgrDI CGTCGACG 1 cut(s) 553
SinI GGWCC 1 cut(s) 8
SsiI CCGC 7 cut(s) 63, 111, 139, 315, 354, 408, 536
SspI AATATT 1 cut(s) 604
StyD4I CCNGG 1 cut(s) 669
StyI CCWWGG 2 cut(s) 27, 582
TaaI ACNGT 3 cut(s) 162, 559, 596
TaqI TCGA 1 cut(s) 554
TauI GCSGC 2 cut(s) 410, 538
TfiI GAWTC 1 cut(s) 486
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 599, 695
TseFI GTSAC 3 cut(s) 31, 196, 617
TseI GCWGC 6 cut(s) 54, 305, 432, 464, 538, 541
Tsp45I GTSAC 3 cut(s) 31, 196, 617
TspDTI ATGAA 1 cut(s) 369
TspGWI ACGGA 2 cut(s) 89, 184
TspRI CASTG 2 cut(s) 599, 695
Tth111I GACNNNGTC 1 cut(s) 421
VpaK11BI GGWCC 1 cut(s) 8
XagI CCTNNNNNAGG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.