Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0009143

Physical Location & Seq

Genomic Coordinates Reverse (-)

27719593 .. 27725779

Gene Structure 6187 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g26260.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGACGTCGACGCCGTCCTCCTCCGCCACCACCAACGTCATGCTGGCCATCTTCGAGAAGAAGACCACCCAAATCGACCTCTACCGCCCCCTCCGCAACTACATCGCCTTCCACTACTCCGAGCGCGAAGCCCAGAACCTCGAGGACGACCTCCAAACCCTAAAACAGCTCCGCTCCGACTTGGAGCGCACACCCGACCCGTCCCTCCCGGCCCGCCGCGACCTCCTCCAAAATTACTACAAAGCCCTCTGCCTCGTCGAGACTCGCTTCCCTATCTCGCCGGACAAGGACCACATCAACTCCGTCACCTTCGTCTGGCACGACGCCTTCAAGAACAAGCAGAAGGCCTCGCAGCAGAACATCCACCTCGAGAAGGCCGCCGTGCTCTTCAATCTCGGCGCCGTCTACAGCCAGATTGGCCTGTCCTACGATCGCGCCACCGTCGACGGAAGGCGCCTGGCGTCTCACGCGTTCATCGCCGCGGCCGGCGCGTTCGCGTTTCTGAGGGACAATGCGGCGACCAAGGCCTCGATCGGAAGCTCCACGACGGTGGATTTGTCGGTGGAGTGCGCCGGGATGCTGGAGAGGCTGATGCTGGCTCAGGCGCAGGAGAGCGTCTTTGAGAATACCATAGCCAAAGGTAGCACGCCTGGTGTTTGCGCCAAGATCTCCAGGCAGGTTGGATTGTACTACGAGGAAGCTTTAGCGGCAGCAAATTCTCCCACTTTGAACCAGCATTTTGACAAGGCTTGGGTATCACACATGCAGTTAAAGGCTGCTCTCTTTTTCGCAGAAGCTTGCTATAGGTATGGTCTGGAGCTACATGAGAAAGAAGAGATTGCAGAAGAGATTGCACGGTTAAAGAGTGGGATAAGTGCTCTAGCGGAGTCTAAGAAATCAACTAGGGGGGCAGCTCAACAAATTTTGGATGCAATTAGCAAGTTAGAGGCCAACCTTAACCGTAACCTTGAGAGGGCTATGAAGGAGAATGATAGAGTTTACCTCATGAGGGTTCCTTCTGTTAGCTCCTTACCACCACTGCCAGCATTTGCCATGGTGAAGCCTATGGCGATGAGTGAGGTCTTGGATGCAAGCAAGGAGAAGATGTTTGCTAGTCTTGTTCCGGACAGTAGTGCAAAAGCTCTTTCAAGGTACACCGAAATGGTCGATGATATTATCAGGACACAAGCTGAAAAATTGCAACAAGCCAGTGAGCTGACTAGAGTAAGGCTCAAGGAAATGGACCTTCCAGAGTCTATTCTTGCTTTGGAAGGAAATTTTACTCTTCCTACGGATCTTAAAGAAGATGTGGAGGCAGTGCAGATTAGTGGGGGCCCTGCTGGTTTGGAAGCTGAGCTGCAGCAACTTAGGGATTTGAGAAGAGTGAACCAGGAAATTTTGGTACACATTGATGAGCTCTTGCAGAAAGAAGCCAGAGAAGATTCCCAGTTTAGAAGCCAATTTGGAACACGGTGGACAAGGCCTCAGTCAAGCACTCTTACTAAGAACTTACAGGATAGGCTAAGTAGGTTTGCAGCTAACTTGAAGCAAGCTTCAGACAGTGATGCTCGGATTGAGCGCTCAGTCAGAGAACATTCAGCTCTCATGTCCATCCTTGATCGTCACCCGATTGAATCTGCCCTCCCAACTCTGGCTAGGCCGATCATGTCTTTGGATGCCAATGAAGATGCTATATTAGGGGCCCTGAAGCAAAGCTTGCGGCAATTGGAAACGCTTGGTGCTCAACGAGCAGGCCTTGAAGACATGCTCAAAGAGATGAAAAGAAAGGATGACATACTTCCTAAGTTGATGACATCAACTGGCTCCTATGAGGATCTTTTCAGGAAGGAGATATCAAAATATGATCTTATAAGTGAAGATATTGGCCACAATATTGAGGCACAAGAACAATTATTGCTGCAAATCCAGGCCCAAAATGATGAGTTTGCTGCAGTCTTCAATCTTGAAGATTACAAAGCATCTCGTGAGAAATGTTACAAGCAGATTCAAGCTGCCATAGGAAAGTACCGTGAGATTAAGGATAACATAAACGAGGGTTTGAAGTTTTATGTTACTCTTCAGGATGCAATAACTAATGTAAAGCAGCAGTGCAGTGACTTTGTGATGACTAGAAATATCCAGTGCCAGGAAATGATTGAAGATGTGCACAAACAGATGGCAGGTCTCAATTTCCAGGATTCTAAAAACTCAGCTGCGTACAACTATCCTTCTGCGGGAAGTCAAAGTCAAAGATCTGGTCCACAACAGCAAACTGATCCACGTCCTCAAACATCCTACTACCGACCACCTGAGCAGCCAGGTTATTCTCAGCCTCCCCCCTACTCAACGCAGCAGATGCCTCCTCCGTACCATGCACCTCCTCAGGCTGGTAGTCCATACCCTCCCCATCAAGCTGGTAGTCCATACCCACCCCCTCAAGCTGGTAGTCCATACCCACCTCCACAGCAACAGCCCCCTGCAAGCCATGAATATGGTCAACCTGCTTATCCAGGGTGGCGGGGTCCATACTACAACGCACAACAATCTGGATCGCATCCGCGACCTCCTTACACCATCCCACCTCAATACCCTCCAAACCAGAGTGGCTACTATAAACCACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005829 GO:0005911 GO:0006996 GO:0007032 GO:0007033 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0010256 GO:0012505 GO:0016043 GO:0016050 GO:0022603 GO:0030054 GO:0031156 GO:0031285 GO:0031286 GO:0031410 GO:0031982 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032182 GO:0032501 GO:0032502 GO:0036257 GO:0042173 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043621 GO:0043900 GO:0043901 GO:0043937 GO:0043938 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045881 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051241 GO:0055044 GO:0065007 GO:0071840 GO:0075260 GO:0075262 GO:0097708 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ALIX_LYPXL_bnd BRO1

Protein Analysis

875

Amino Acids

98.03

Weight (kDa)

6.1

Isoelectric Point (pI)

60.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRO1	PF03097	14 - 393	2e-108	BRO1-like domain
ALIX_LYPXL_bnd	PF13949	428 - 713	2.8e-75	ALIX V-shaped domain binding to HIV

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15130
fragaria_vesca	FvH4_4g26260 FvH4_4g26260
malus_domestica	MD13G1031100.v1.1 MD16G1033300.v1.1
prunus_persica	Prupe.1G321000_v2.0.a1 Prupe.1G321000_v2.0.a1
pyrus_communis	pycom13g02720 pycom16g02790
rosa_chinensis	RchiOBHm_Chr4g0433861
rosa_laevigata	RLG00000006722
rosa_multiflora	Rmu_co8294453.1_g000001 Rmu_sc0000352.1_g000001 Rmu_ssc0000164.1_g000001
rosa_roxburghii	Rroxscaffold_5G00375260
rosa_rugosa	Rorug04G0271800 Rorug04G0271900
rosa_samantha	Rh4AG326600 Rh4BG334000 Rh4CG349700 Rh4DG330800
rosa_wichuraiana	Rw4G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1871
AatII	GACGTC	1 cut(s)	8
AbsI	CCTCGAGG	1 cut(s)	140
Acc36I	ACCTGC	3 cut(s)	667, 2171, 2509
AccB1I	GGYRCC	2 cut(s)	398, 453
AccBSI	CCGCTC	1 cut(s)	174
AccI	GTMKAC	3 cut(s)	8, 405, 444
AccII	CGCG	8 cut(s)	126, 219, 435, 470, 482, 491, 497, 2560
AccIII	TCCGGA	1 cut(s)	1123
AclWI	GGATC	4 cut(s)	1302, 1842, 2270, 2557
AcoI	YGGCCR	3 cut(s)	45, 483, 1885
AcsI	RAATTY	4 cut(s)	715, 921, 1276, 1395
AcuI	CTGAAG	3 cut(s)	1539, 1727, 2063
AcyI	GRCGYC	6 cut(s)	5, 11, 324, 399, 454, 461
AfaI	GTAC	6 cut(s)	689, 1154, 1404, 2027, 2219, 2369
AfeI	AGCGCT	1 cut(s)	1580
AfiI	CCNNNNNNNGG	9 cut(s)	315, 373, 973, 1009, 1651, 2146, 2234, 2387, 2441
AflIII	ACRYGT	1 cut(s)	468
AjiI	CACGTC	1 cut(s)	2282
AjnI	CCWGG	9 cut(s)	456, 649, 671, 1389, 1926, 2145, 2193, 2317, 2509
AleI	CACNNNNGTG	1 cut(s)	548
Alw21I	GWGCWC	5 cut(s)	387, 880, 1419, 1744, 2169
Alw26I	GTCTC	3 cut(s)	254, 468, 2189
Alw44I	GTGCAC	1 cut(s)	2165
AlwI	GGATC	4 cut(s)	1302, 1842, 2270, 2557
Ama87I	CYCGRG	2 cut(s)	140, 368
Aor13HI	TCCGGA	1 cut(s)	1123
Aor51HI	AGCGCT	1 cut(s)	1580
ApaI	GGGCCC	2 cut(s)	1337, 1705
ApaLI	GTGCAC	1 cut(s)	2165
ApoI	RAATTY	4 cut(s)	715, 921, 1276, 1395
ArsI	GACNNNNNNTTYG	4 cut(s)	538, 570, 2242, 2274
AsuC2I	CCSGG	2 cut(s)	209, 574
AsuHPI	GGTGA	3 cut(s)	298, 1069, 1616
AvaI	CYCGRG	2 cut(s)	140, 368
AvaII	GGWCC	4 cut(s)	289, 1243, 2258, 2522
AxyI	CCTNAGG	1 cut(s)	2382
BaeGI	GKGCMC	3 cut(s)	1337, 1705, 2169
BalI	TGGCCA	2 cut(s)	47, 1887
BanI	GGYRCC	2 cut(s)	398, 453
BanII	GRGCYC	3 cut(s)	1337, 1419, 1705
BauI	CACGAG	1 cut(s)	1983
BbsI	GAAGAC	3 cut(s)	68, 1767, 1948
Bbv12I	GWGCWC	5 cut(s)	387, 880, 1419, 1744, 2169
BccI	CCATC	5 cut(s)	56, 1619, 2170, 2415, 2582
BceAI	ACGGC	2 cut(s)	365, 386
BcgI	CGANNNNNNTGC	2 cut(s)	85, 119
BciT130I	CCWGG	9 cut(s)	458, 651, 673, 1391, 1928, 2147, 2195, 2319, 2511
BcnI	CCSGG	2 cut(s)	209, 574
BcoDI	GTCTC	3 cut(s)	254, 468, 2189
BfaI	CTAG	6 cut(s)	881, 903, 1113, 1221, 1656, 2130
BfmI	CTRYAG	4 cut(s)	406, 802, 1358, 1950
BfoI	RGCGCY	3 cut(s)	402, 457, 1582
BfuAI	ACCTGC	3 cut(s)	667, 2171, 2509
BglI	GCCNNNNNGGC	1 cut(s)	417
BglII	AGATCT	2 cut(s)	666, 2252
BlpI	GCTNAGC	1 cut(s)	1353
Bme18I	GGWCC	4 cut(s)	289, 1243, 2258, 2522
BmeT110I	CYCGRG	2 cut(s)	140, 368
BmgBI	CACGTC	1 cut(s)	2282
BmiI	GGNNCC	9 cut(s)	400, 455, 1014, 1334, 1335, 1702, 1703, 1825, 2523
BmrI	ACTGGG	1 cut(s)	1441
BmuI	ACTGGG	1 cut(s)	1441
BpiI	GAAGAC	3 cut(s)	68, 1767, 1948
BplI	GAGNNNNNCTC	4 cut(s)	987, 1019, 1215, 1247
BpmI	CTGGAG	3 cut(s)	602, 655, 836
Bpu10I	CCTNAGC	2 cut(s)	600, 2310
Bpu1102I	GCTNAGC	1 cut(s)	1353
BpuEI	CTTGAG	3 cut(s)	989, 1217, 2421
BpuMI	CCSGG	2 cut(s)	209, 574
BsaHI	GRCGYC	6 cut(s)	5, 11, 324, 399, 454, 461
BsaI	GGTCTC	1 cut(s)	2189
BsaJI	CCNNGG	4 cut(s)	480, 522, 1053, 2510
BsaWI	WCCGGW	1 cut(s)	1123
BsaXI	ACNNNNNCTCC	4 cut(s)	284, 314, 1091, 1121
Bsc4I	CCNNNNNNNGG	9 cut(s)	315, 373, 973, 1009, 1651, 2146, 2234, 2387, 2441
Bse118I	RCCGGY	1 cut(s)	485
Bse1I	ACTGG	4 cut(s)	1209, 1447, 1825, 2140
Bse21I	CCTNAGG	1 cut(s)	2382
BseAI	TCCGGA	1 cut(s)	1123
BseBI	CCWGG	9 cut(s)	458, 651, 673, 1391, 1928, 2147, 2195, 2319, 2511
BseDI	CCNNGG	4 cut(s)	480, 522, 1053, 2510
BseLI	CCNNNNNNNGG	9 cut(s)	315, 373, 973, 1009, 1651, 2146, 2234, 2387, 2441
BseMII	CTCAG	9 cut(s)	494, 614, 1344, 1499, 1596, 2223, 2301, 2342, 2396
BseNI	ACTGG	4 cut(s)	1209, 1447, 1825, 2140
BseRI	GAGGAG	4 cut(s)	10, 215, 2352, 2370
BseSI	GKGCMC	3 cut(s)	1337, 1705, 2169
BseX3I	CGGCCG	1 cut(s)	483
BsgI	GTGCAG	2 cut(s)	1340, 2131
Bsh1236I	CGCG	8 cut(s)	126, 219, 435, 470, 482, 491, 497, 2560
Bsh1285I	CGRYCG	3 cut(s)	433, 486, 534
BshNI	GGYRCC	2 cut(s)	398, 453
BsiEI	CGRYCG	3 cut(s)	433, 486, 534
BsiHKAI	GWGCWC	5 cut(s)	387, 880, 1419, 1744, 2169
BsiHKCI	CYCGRG	2 cut(s)	140, 368
BsiSI	CCGG	5 cut(s)	209, 281, 486, 573, 1124
BslFI	GGGAC	2 cut(s)	187, 521
BslI	CCNNNNNNNGG	9 cut(s)	315, 373, 973, 1009, 1651, 2146, 2234, 2387, 2441
BsmAI	GTCTC	3 cut(s)	254, 468, 2189
BsmBI	CGTCTC	1 cut(s)	468
BsmFI	GGGAC	2 cut(s)	187, 521
Bso31I	GGTCTC	1 cut(s)	2189
BsoBI	CYCGRG	2 cut(s)	140, 368
Bsp120I	GGGCCC	2 cut(s)	1333, 1701
Bsp1286I	GDGCHC	7 cut(s)	387, 880, 1337, 1419, 1705, 1744, 2169
Bsp13I	TCCGGA	1 cut(s)	1123
Bsp1720I	GCTNAGC	1 cut(s)	1353
Bsp19I	CCATGG	1 cut(s)	1053
BspCNI	CTCAG	9 cut(s)	495, 613, 1345, 1498, 1595, 2222, 2302, 2341, 2395
BspEI	TCCGGA	1 cut(s)	1123
BspFNI	CGCG	8 cut(s)	126, 219, 435, 470, 482, 491, 497, 2560
BspHI	TCATGA	1 cut(s)	1005
BspLI	GGNNCC	9 cut(s)	400, 455, 1014, 1334, 1335, 1702, 1703, 1825, 2523
BspMAI	CTGCAG	2 cut(s)	1362, 1954
BspMI	ACCTGC	3 cut(s)	667, 2171, 2509
BspPI	GGATC	4 cut(s)	1302, 1842, 2270, 2557
BspQI	GCTCTTC	1 cut(s)	392
BspT107I	GGYRCC	2 cut(s)	398, 453
BspTNI	GGTCTC	1 cut(s)	2189
BsrBI	CCGCTC	1 cut(s)	174
BsrFI	RCCGGY	1 cut(s)	485
BsrI	ACTGG	4 cut(s)	1209, 1447, 1825, 2140
BssAI	RCCGGY	1 cut(s)	485
BssECI	CCNNGG	4 cut(s)	480, 522, 1053, 2510
BssNI	GRCGYC	6 cut(s)	5, 11, 324, 399, 454, 461
BssSI	CACGAG	1 cut(s)	1983
BssT1I	CCWWGG	2 cut(s)	522, 1053
Bst2BI	CACGAG	1 cut(s)	1983
Bst2UI	CCWGG	9 cut(s)	458, 651, 673, 1391, 1928, 2147, 2195, 2319, 2511
Bst4CI	ACNGT	8 cut(s)	442, 550, 858, 962, 1130, 1473, 1562, 2030
Bst6I	CTCTTC	6 cut(s)	392, 828, 840, 1290, 1375, 2082
BstACI	GRCGYC	6 cut(s)	5, 11, 324, 399, 454, 461
BstAPI	GCANNNNNTGC	2 cut(s)	1717, 2356
BstDSI	CCRYGG	2 cut(s)	480, 1053
BstENI	CCTNNNNNAGG	1 cut(s)	371
BstFNI	CGCG	8 cut(s)	126, 219, 435, 470, 482, 491, 497, 2560
BstH2I	RGCGCY	3 cut(s)	402, 457, 1582
BstMAI	GTCTC	3 cut(s)	254, 468, 2189
BstMCI	CGRYCG	3 cut(s)	433, 486, 534
BstNI	CCWGG	9 cut(s)	458, 651, 673, 1391, 1928, 2147, 2195, 2319, 2511
BstNSI	RCATGY	2 cut(s)	766, 1768
BstSFI	CTRYAG	4 cut(s)	406, 802, 1358, 1950
BstSLI	GKGCMC	3 cut(s)	1337, 1705, 2169
BstUI	CGCG	8 cut(s)	126, 219, 435, 470, 482, 491, 497, 2560
BstV2I	GAAGAC	3 cut(s)	68, 1767, 1948
BstX2I	RGATCY	4 cut(s)	666, 1294, 1834, 2252
BstXI	CCANNNNNNTGG	3 cut(s)	550, 2414, 2492
BstYI	RGATCY	4 cut(s)	666, 1294, 1834, 2252
BstZI	CGGCCG	1 cut(s)	483
Bsu36I	CCTNAGG	1 cut(s)	2382
BtgI	CCRYGG	2 cut(s)	480, 1053
BtgZI	GCGATG	3 cut(s)	88, 460, 1085
BtrI	CACGTC	1 cut(s)	2282
BtsI	GCAGTG	4 cut(s)	1037, 1323, 2114, 2119
BtsIMutI	CAGTG	7 cut(s)	1037, 1216, 1323, 1567, 2114, 2119, 2147
BveI	ACCTGC	3 cut(s)	667, 2171, 2509
CciI	TCATGA	1 cut(s)	1005
Cfr10I	RCCGGY	1 cut(s)	485
Cfr42I	CCGCGG	1 cut(s)	483
CseI	GACGC	4 cut(s)	19, 332, 450, 604
Csp6I	GTAC	6 cut(s)	688, 1153, 1403, 2026, 2218, 2368
CviQI	GTAC	6 cut(s)	688, 1153, 1403, 2026, 2218, 2368
DinI	GGCGCC	2 cut(s)	400, 455
EaeI	YGGCCR	3 cut(s)	45, 483, 1885
EagI	CGGCCG	1 cut(s)	483
Eam1104I	CTCTTC	6 cut(s)	392, 828, 840, 1290, 1375, 2082
EarI	CTCTTC	6 cut(s)	392, 828, 840, 1290, 1375, 2082
EciI	GGCGGA	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	1417
EclXI	CGGCCG	1 cut(s)	483
Eco130I	CCWWGG	2 cut(s)	522, 1053
Eco147I	AGGCCT	4 cut(s)	347, 527, 1483, 1755
Eco24I	GRGCYC	3 cut(s)	1337, 1419, 1705
Eco31I	GGTCTC	1 cut(s)	2189
Eco32I	GATATC	1 cut(s)	1854
Eco47I	GGWCC	4 cut(s)	289, 1243, 2258, 2522
Eco47III	AGCGCT	1 cut(s)	1580
Eco52I	CGGCCG	1 cut(s)	483
Eco53kI	GAGCTC	1 cut(s)	1417
Eco57I	CTGAAG	3 cut(s)	1539, 1727, 2063
Eco81I	CCTNAGG	1 cut(s)	2382
Eco88I	CYCGRG	2 cut(s)	140, 368
EcoICRI	GAGCTC	1 cut(s)	1417
EcoNI	CCTNNNNNAGG	1 cut(s)	371
EcoO109I	RGGNCCY	4 cut(s)	1333, 1334, 1701, 1702
EcoRII	CCWGG	9 cut(s)	456, 649, 671, 1389, 1926, 2145, 2193, 2317, 2509
EcoRV	GATATC	1 cut(s)	1854
EcoT14I	CCWWGG	2 cut(s)	522, 1053
EcoT38I	GRGCYC	3 cut(s)	1337, 1419, 1705
EgeI	GGCGCC	2 cut(s)	400, 455
EheI	GGCGCC	2 cut(s)	400, 455
ErhI	CCWWGG	2 cut(s)	522, 1053
Esp3I	CGTCTC	1 cut(s)	468
FalI	AAGNNNNNCTT	2 cut(s)	1700, 1732
FaqI	GGGAC	2 cut(s)	187, 521
FauI	CCCGC	3 cut(s)	221, 2227, 2511
FblI	GTMKAC	3 cut(s)	8, 405, 444
FriOI	GRGCYC	3 cut(s)	1337, 1419, 1705
FspBI	CTAG	6 cut(s)	881, 903, 1113, 1221, 1656, 2130
GsuI	CTGGAG	3 cut(s)	602, 655, 836
HaeII	RGCGCY	3 cut(s)	402, 457, 1582
HapII	CCGG	5 cut(s)	209, 281, 486, 573, 1124
HgaI	GACGC	4 cut(s)	19, 332, 450, 604
Hin1I	GRCGYC	6 cut(s)	5, 11, 324, 399, 454, 461
HincII	GTYRAC	3 cut(s)	9, 445, 2498
HindII	GTYRAC	3 cut(s)	9, 445, 2498
HindIII	AAGCTT	4 cut(s)	699, 795, 1551, 1714
HinfI	GANTC	7 cut(s)	262, 887, 1253, 1442, 1634, 2005, 2198
HpaII	CCGG	5 cut(s)	209, 281, 486, 573, 1124
HphI	GGTGA	3 cut(s)	298, 1069, 1616
Hpy188I	TCNGA	7 cut(s)	121, 178, 504, 536, 1558, 1572, 1589
Hpy99I	CGWCG	7 cut(s)	10, 13, 260, 326, 446, 449, 550
HpyCH4III	ACNGT	8 cut(s)	442, 550, 858, 962, 1130, 1473, 1562, 2030
HpyCH4IV	ACGT	3 cut(s)	5, 36, 2281
HpySE526I	ACGT	3 cut(s)	5, 36, 2281
Hsp92I	GRCGYC	6 cut(s)	5, 11, 324, 399, 454, 461
KasI	GGCGCC	2 cut(s)	398, 453
Kpn2I	TCCGGA	1 cut(s)	1123
KroI	GCCGGC	1 cut(s)	485
KroNI	GCCGGC	1 cut(s)	487
KspI	CCGCGG	1 cut(s)	483
LguI	GCTCTTC	1 cut(s)	392
LmnI	GCTCC	7 cut(s)	174, 179, 184, 545, 817, 1031, 1829
MaeI	CTAG	6 cut(s)	881, 903, 1113, 1221, 1656, 2130
MaeII	ACGT	3 cut(s)	5, 36, 2281
MaeIII	GTNAC	6 cut(s)	304, 962, 1622, 1994, 2071, 2114
MbiI	CCGCTC	1 cut(s)	174
MfeI	CAATTG	1 cut(s)	1724
MflI	RGATCY	4 cut(s)	666, 1294, 1834, 2252
MhlI	GDGCHC	7 cut(s)	387, 880, 1337, 1419, 1705, 1744, 2169
MlsI	TGGCCA	2 cut(s)	47, 1887
MluI	ACGCGT	1 cut(s)	468
MluNI	TGGCCA	2 cut(s)	47, 1887
Mly113I	GGCGCC	2 cut(s)	399, 454
MlyI	GAGTC	3 cut(s)	256, 896, 1262
MmeI	TCCRAC	2 cut(s)	201, 661
Mox20I	TGGCCA	2 cut(s)	47, 1887
MroI	TCCGGA	1 cut(s)	1123
MroNI	GCCGGC	1 cut(s)	485
MscI	TGGCCA	2 cut(s)	47, 1887
MseI	TTAA	5 cut(s)	770, 860, 957, 1299, 2037
MslI	CAYNNNNRTG	4 cut(s)	548, 1160, 1410, 2490
Msp20I	TGGCCA	2 cut(s)	47, 1887
MspA1I	CMGCKG	2 cut(s)	482, 2213
MspI	CCGG	5 cut(s)	209, 281, 486, 573, 1124
MunI	CAATTG	1 cut(s)	1724
MvaI	CCWGG	9 cut(s)	458, 651, 673, 1391, 1928, 2147, 2195, 2319, 2511
MvnI	CGCG	8 cut(s)	126, 219, 435, 470, 482, 491, 497, 2560
NaeI	GCCGGC	1 cut(s)	487
NarI	GGCGCC	2 cut(s)	399, 454
NciI	CCSGG	2 cut(s)	209, 574
NcoI	CCATGG	1 cut(s)	1053
NgoMIV	GCCGGC	1 cut(s)	485
NlaIV	GGNNCC	9 cut(s)	400, 455, 1014, 1334, 1335, 1702, 1703, 1825, 2523
NmeAIII	GCCGAG	1 cut(s)	375
NmuCI	GTSAC	3 cut(s)	304, 1622, 2114
NspI	RCATGY	2 cut(s)	766, 1768
OliI	CACNNNNGTG	1 cut(s)	548
PaeR7I	CTCGAG	2 cut(s)	140, 368
PagI	TCATGA	1 cut(s)	1005
PceI	AGGCCT	4 cut(s)	347, 527, 1483, 1755
PciSI	GCTCTTC	1 cut(s)	392
PcsI	WCGNNNNNNNCGW	2 cut(s)	11, 318
PdiI	GCCGGC	1 cut(s)	487
PfeI	GAWTC	4 cut(s)	1442, 1634, 2005, 2198
PflFI	GACNNNGTC	1 cut(s)	13
PfoI	TCCNGGA	1 cut(s)	2193
Ple19I	CGATCG	2 cut(s)	433, 534
PleI	GAGTC	3 cut(s)	256, 895, 1261
PluTI	GGCGCC	2 cut(s)	402, 457
PpsI	GAGTC	3 cut(s)	256, 895, 1261
PsiI	TTATAA	1 cut(s)	1871
Psp124BI	GAGCTC	1 cut(s)	1419
Psp6I	CCWGG	9 cut(s)	456, 649, 671, 1389, 1926, 2145, 2193, 2317, 2509
PspGI	CCWGG	9 cut(s)	456, 649, 671, 1389, 1926, 2145, 2193, 2317, 2509
PspN4I	GGNNCC	9 cut(s)	400, 455, 1014, 1334, 1335, 1702, 1703, 1825, 2523
PspOMI	GGGCCC	2 cut(s)	1333, 1701
PspXI	VCTCGAGB	1 cut(s)	140
PstI	CTGCAG	2 cut(s)	1362, 1954
PsuI	RGATCY	4 cut(s)	666, 1294, 1834, 2252
PsyI	GACNNNGTC	1 cut(s)	13
PvuI	CGATCG	2 cut(s)	433, 534
PvuII	CAGCTG	1 cut(s)	2213
RsaI	GTAC	6 cut(s)	689, 1154, 1404, 2027, 2219, 2369
RsaNI	GTAC	6 cut(s)	688, 1153, 1403, 2026, 2218, 2368
RseI	CAYNNNNRTG	4 cut(s)	548, 1160, 1410, 2490
SacI	GAGCTC	1 cut(s)	1419
SacII	CCGCGG	1 cut(s)	483
SalI	GTCGAC	2 cut(s)	7, 443
SapI	GCTCTTC	1 cut(s)	392
SaqAI	TTAA	5 cut(s)	770, 860, 957, 1299, 2037
SchI	GAGTC	3 cut(s)	256, 896, 1262
SduI	GDGCHC	7 cut(s)	387, 880, 1337, 1419, 1705, 1744, 2169
SfcI	CTRYAG	4 cut(s)	406, 802, 1358, 1950
SfoI	GGCGCC	2 cut(s)	400, 455
Sfr274I	CTCGAG	2 cut(s)	140, 368
Sfr303I	CCGCGG	1 cut(s)	483
SgrBI	CCGCGG	1 cut(s)	483
SgrDI	CGTCGACG	2 cut(s)	7, 443
SinI	GGWCC	4 cut(s)	289, 1243, 2258, 2522
SlaI	CTCGAG	2 cut(s)	140, 368
SmiMI	CAYNNNNRTG	4 cut(s)	548, 1160, 1410, 2490
SmlI	CTYRAG	5 cut(s)	140, 368, 968, 1232, 2436
SmoI	CTYRAG	5 cut(s)	140, 368, 968, 1232, 2436
SseBI	AGGCCT	4 cut(s)	347, 527, 1483, 1755
SspDI	GGCGCC	2 cut(s)	398, 453
SspI	AATATT	1 cut(s)	1894
SspMI	CTAG	6 cut(s)	881, 903, 1113, 1221, 1656, 2130
SstI	GAGCTC	1 cut(s)	1419
StuI	AGGCCT	4 cut(s)	347, 527, 1483, 1755
StyI	CCWWGG	2 cut(s)	522, 1053
TaaI	ACNGT	8 cut(s)	442, 550, 858, 962, 1130, 1473, 1562, 2030
TaiI	ACGT	3 cut(s)	8, 39, 2284
TaqI	TCGA	9 cut(s)	8, 54, 75, 141, 258, 369, 444, 530, 1167
TatI	WGTACW	1 cut(s)	687
TauI	GCSGC	7 cut(s)	219, 380, 482, 485, 518, 710, 1723
TfiI	GAWTC	4 cut(s)	1442, 1634, 2005, 2198
Tru1I	TTAA	5 cut(s)	770, 860, 957, 1299, 2037
Tru9I	TTAA	5 cut(s)	770, 860, 957, 1299, 2037
TscAI	CASTG	7 cut(s)	1044, 1216, 1323, 1567, 2114, 2119, 2147
TseFI	GTSAC	3 cut(s)	304, 1622, 2114
Tsp45I	GTSAC	3 cut(s)	304, 1622, 2114
TspDTI	ATGAA	5 cut(s)	463, 995, 1698, 1793, 2502
TspGWI	ACGGA	4 cut(s)	292, 462, 1307, 2355
TspRI	CASTG	7 cut(s)	1044, 1216, 1323, 1567, 2114, 2119, 2147
Tth111I	GACNNNGTC	1 cut(s)	13
VneI	GTGCAC	1 cut(s)	2165
VpaK11BI	GGWCC	4 cut(s)	289, 1243, 2258, 2522
XagI	CCTNNNNNAGG	1 cut(s)	371
XapI	RAATTY	4 cut(s)	715, 921, 1276, 1395
XceI	RCATGY	2 cut(s)	766, 1768
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XhoI	CTCGAG	2 cut(s)	140, 368
XmiI	GTMKAC	3 cut(s)	8, 405, 444
XspI	CTAG	6 cut(s)	881, 903, 1113, 1221, 1656, 2130
ZraI	GACGTC	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.