Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0009143

Physical Location & Seq

Genomic Coordinates Reverse (-)

1879831 .. 1882358

Gene Structure 2528 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g02720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGCTGGAGAGGCTGATGCTGGCGCAGGCCCAAGAGTGCGTCTTTGAGAATACTATTGCCAAAGGGAGCACGCCAGGTGTTTGTGCCAAGATCTCGAGGCAGGTTGGGCTGTATTACGAGGAAGCTTTAGCGACATGTAATGCTCCGCCCTTGTACCAGCATTTTGACAGGGCCTGGATATCACACATGCAGTTAAAGGCAGCTCTCTTTTATGCAGAAGCTTGCTATAGGTTTGGTATAGAGCTCCATGAGAAAGAAGAGATTGCAGAAGAAATTGCACGGTTAAAGAGTGGGATTAGTGCTTTGTCTGAGGCTAAGAAAAACACAAAGGGGGCTGCTGCACAGATTCTGGACGCAATTAGTAAGTTAGATGTCCATTTGAACCGTAACCTGGAGAGGGCTGTAAAGGAGAATGATAGAGTTTATCTCATGAGGGTTCCTTCCCCTGATTCCTTACCACCTCTTCCAGCATTTTCCATGGTGAAGCCTTTGGCAATGAGTGAGGTCCTGGATGCGAGCAAGGAGAAGATGTTTGCTAGTCTTGTTCCAGACAGTAGTGCAAAAGCTCTTTCCAGGTACACTGAAATGGTTGATGACATTATCAGAACACAAGCAGAAAAGCTACAACAAGCCAGTGAACTGACCAGAGTAAGGCTCAAGGAAATGGACCTTCCGGAGTCTATTCTTGCATTGGAAGGGAATTTTACTCTTCCTACAGATCTTAAAGAAGACGTGGAGGCAGTCCAGATATGTGGAGGCCCTGCTGGTTTGGAAGCTGAGTTACAGCAGCTTAAGGATTTGAAAAGGGTAAACCAGGAAATGCTGGTACAGATTGAGGAACTCTTGCAGAAAGAGGCCAGAGAAGATGCTCAGTTTAGAAGCCAATTTGGAACCCGGTGGACGAGGCCGCAGTCAAGTACTTTGACAAAGAGCTTACAGGATAGGCTAAATAGGTTTGCAGGGAACTTGAAGCAAGCTGCAGATAGTGATGCTCGGATTGAGCGCTCGGTCAGAGAACATTCAGCTCTCATGTCAATCCTTGATCGTCGTCCGATTGAATCTGCCCTCCCAACTCTGGCTAGGCCAATAATGTCTTTGGATGCCAATGAAGATGCTATTTTGGGGGCCCTGAAGCAGAGCTTGAGGCAACTGGAAACCCTTGGTGCCCAACGAGCTGGTCTTGAAGACATGCTTAAAGAGATGAAAAGAAAGGATGATATACTACCTAAGTTGATGACATCAACTGGCTCCTATGAGGATCTTTTCAGGAAGGAGATATCTAAGTATCGATCTTATAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005829 GO:0005911 GO:0006996 GO:0007032 GO:0007033 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0010256 GO:0012505 GO:0016043 GO:0016050 GO:0022603 GO:0030054 GO:0031156 GO:0031285 GO:0031286 GO:0031410 GO:0031982 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032182 GO:0032501 GO:0032502 GO:0036257 GO:0042173 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043621 GO:0043900 GO:0043901 GO:0043937 GO:0043938 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045881 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051241 GO:0055044 GO:0065007 GO:0071840 GO:0075260 GO:0075262 GO:0097708 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ALIX_LYPXL_bnd BRO1

Protein Analysis

434

Amino Acids

48.75

Weight (kDa)

5.96

Isoelectric Point (pI)

52.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRO1	PF03097	1 - 201	1.3e-54	BRO1-like domain
ALIX_LYPXL_bnd	PF13949	236 - 432	2.4e-49	ALIX V-shaped domain binding to HIV

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15130
fragaria_vesca	FvH4_4g26260 FvH4_4g26260
malus_domestica	MD13G1031100.v1.1 MD16G1033300.v1.1
prunus_persica	Prupe.1G321000_v2.0.a1 Prupe.1G321000_v2.0.a1
pyrus_communis	pycom13g02720 pycom16g02790
rosa_chinensis	RchiOBHm_Chr4g0433861
rosa_laevigata	RLG00000006722
rosa_multiflora	Rmu_co8294453.1_g000001 Rmu_sc0000352.1_g000001 Rmu_ssc0000164.1_g000001
rosa_roxburghii	Rroxscaffold_5G00375260
rosa_rugosa	Rorug04G0271800 Rorug04G0271900
rosa_samantha	Rh4AG326600 Rh4BG334000 Rh4CG349700 Rh4DG330800
rosa_wichuraiana	Rw4G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1296
Acc36I	ACCTGC	1 cut(s)	91
AccB1I	GGYRCC	1 cut(s)	1163
AccIII	TCCGGA	1 cut(s)	673
AciI	CCGC	2 cut(s)	146, 908
AclWI	GGATC	1 cut(s)	1266
AcsI	RAATTY	1 cut(s)	700
AcuI	CTGAAG	1 cut(s)	1151
AfaI	GTAC	4 cut(s)	155, 578, 828, 919
AfeI	AGCGCT	1 cut(s)	1004
AfiI	CCNNNNNNNGG	4 cut(s)	391, 397, 651, 1075
AflII	CTTAAG	1 cut(s)	791
AflIII	ACRYGT	1 cut(s)	134
AgsI	TTSAA	5 cut(s)	382, 802, 970, 1058, 1184
AjiI	CACGTC	1 cut(s)	733
AjnI	CCWGG	6 cut(s)	73, 173, 390, 507, 572, 813
Alw21I	GWGCWC	2 cut(s)	71, 246
AlwI	GGATC	1 cut(s)	1266
AlwNI	CAGNNNCTG	2 cut(s)	174, 349
Ama87I	CYCGRG	1 cut(s)	94
Aor13HI	TCCGGA	1 cut(s)	673
Aor51HI	AGCGCT	1 cut(s)	1004
AoxI	GGCC	7 cut(s)	27, 171, 757, 855, 905, 1082, 1125
ApaI	GGGCCC	1 cut(s)	1129
ApeKI	GCWGC	5 cut(s)	200, 335, 338, 787, 977
ApoI	RAATTY	1 cut(s)	700
AspLEI	GCGC	2 cut(s)	25, 1005
AspS9I	GGNCC	7 cut(s)	28, 171, 505, 667, 758, 1125, 1126
AsuC2I	CCSGG	1 cut(s)	895
AsuHPI	GGTGA	1 cut(s)	493
AvaI	CYCGRG	1 cut(s)	94
AvaII	GGWCC	2 cut(s)	505, 667
BaeGI	GKGCMC	2 cut(s)	1129, 1168
BanI	GGYRCC	1 cut(s)	1163
BanII	GRGCYC	2 cut(s)	246, 1129
BarI	GAAGNNNNNNTAC	2 cut(s)	765, 797
BbsI	GAAGAC	2 cut(s)	735, 1191
Bbv12I	GWGCWC	2 cut(s)	71, 246
BbvI	GCAGC	5 cut(s)	212, 322, 325, 799, 964
BciT130I	CCWGG	6 cut(s)	75, 175, 392, 509, 574, 815
BcnI	CCSGG	1 cut(s)	895
BfaI	CTAG	2 cut(s)	537, 1080
BfmI	CTRYAG	3 cut(s)	226, 714, 978
BfoI	RGCGCY	1 cut(s)	1006
BfrI	CTTAAG	1 cut(s)	791
BfuAI	ACCTGC	1 cut(s)	91
BglII	AGATCT	2 cut(s)	90, 718
BisI	GCNGC	6 cut(s)	201, 336, 339, 788, 908, 978
BlsI	GCNGC	6 cut(s)	202, 337, 340, 789, 909, 979
BmcAI	AGTACT	1 cut(s)	919
Bme1390I	CCNGG	7 cut(s)	75, 175, 392, 509, 574, 815, 895
Bme18I	GGWCC	2 cut(s)	505, 667
BmeT110I	CYCGRG	1 cut(s)	94
BmgBI	CACGTC	1 cut(s)	733
BmgT120I	GGNCC	7 cut(s)	28, 171, 505, 667, 758, 1125, 1126
BmiI	GGNNCC	6 cut(s)	438, 892, 1126, 1127, 1165, 1249
BmrFI	CCNGG	7 cut(s)	75, 175, 392, 509, 574, 815, 895
BmsI	GCATC	6 cut(s)	6, 502, 856, 979, 1090, 1102
BpiI	GAAGAC	2 cut(s)	735, 1191
BplI	GAGNNNNNCTC	4 cut(s)	411, 443, 639, 671
BpmI	CTGGAG	2 cut(s)	26, 413
BpuEI	CTTGAG	2 cut(s)	641, 1162
BpuMI	CCSGG	1 cut(s)	895
Bsa29I	ATCGAT	1 cut(s)	1288
BsaJI	CCNNGG	2 cut(s)	477, 1159
BsaWI	WCCGGW	1 cut(s)	673
BsaXI	ACNNNNNCTCC	2 cut(s)	515, 545
Bsc4I	CCNNNNNNNGG	4 cut(s)	391, 397, 651, 1075
Bse1I	ACTGG	3 cut(s)	633, 1155, 1249
Bse3DI	GCAATG	1 cut(s)	501
BseAI	TCCGGA	1 cut(s)	673
BseBI	CCWGG	6 cut(s)	75, 175, 392, 509, 574, 815
BseCI	ATCGAT	1 cut(s)	1288
BseDI	CCNNGG	2 cut(s)	477, 1159
BseGI	GGATG	3 cut(s)	517, 1105, 1219
BseLI	CCNNNNNNNGG	4 cut(s)	391, 397, 651, 1075
BseMI	GCAATG	1 cut(s)	501
BseMII	CTCAG	3 cut(s)	300, 768, 884
BseNI	ACTGG	3 cut(s)	633, 1155, 1249
BseSI	GKGCMC	2 cut(s)	1129, 1168
BseXI	GCAGC	5 cut(s)	212, 322, 325, 799, 964
BsgI	GTGCAG	1 cut(s)	324
BshFI	GGCC	7 cut(s)	29, 173, 759, 857, 907, 1084, 1127
BshNI	GGYRCC	1 cut(s)	1163
BshVI	ATCGAT	1 cut(s)	1288
BsiHKAI	GWGCWC	2 cut(s)	71, 246
BsiHKCI	CYCGRG	1 cut(s)	94
BsiSI	CCGG	2 cut(s)	674, 895
BslI	CCNNNNNNNGG	4 cut(s)	391, 397, 651, 1075
BsnI	GGCC	7 cut(s)	29, 173, 759, 857, 907, 1084, 1127
BsoBI	CYCGRG	1 cut(s)	94
Bsp120I	GGGCCC	1 cut(s)	1125
Bsp1286I	GDGCHC	4 cut(s)	71, 246, 1129, 1168
Bsp13I	TCCGGA	1 cut(s)	673
Bsp143I	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
Bsp19I	CCATGG	1 cut(s)	477
BspACI	CCGC	2 cut(s)	146, 908
BspANI	GGCC	7 cut(s)	29, 173, 759, 857, 907, 1084, 1127
BspCNI	CTCAG	3 cut(s)	301, 769, 883
BspDI	ATCGAT	1 cut(s)	1288
BspEI	TCCGGA	1 cut(s)	673
BspHI	TCATGA	1 cut(s)	429
BspLI	GGNNCC	6 cut(s)	438, 892, 1126, 1127, 1165, 1249
BspMAI	CTGCAG	1 cut(s)	982
BspMI	ACCTGC	1 cut(s)	91
BspPI	GGATC	1 cut(s)	1266
BspT107I	GGYRCC	1 cut(s)	1163
BspTI	CTTAAG	1 cut(s)	791
BsrDI	GCAATG	1 cut(s)	501
BsrI	ACTGG	3 cut(s)	633, 1155, 1249
BssECI	CCNNGG	2 cut(s)	477, 1159
BssMI	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
BssT1I	CCWWGG	2 cut(s)	477, 1159
Bst2UI	CCWGG	6 cut(s)	75, 175, 392, 509, 574, 815
Bst4CI	ACNGT	3 cut(s)	282, 386, 554
Bst6I	CTCTTC	3 cut(s)	252, 468, 714
BstAFI	CTTAAG	1 cut(s)	791
BstC8I	GCNNGC	6 cut(s)	21, 27, 71, 223, 517, 975
BstDEI	CTNAG	6 cut(s)	309, 315, 777, 870, 1227, 1281
BstDSI	CCRYGG	1 cut(s)	477
BstF5I	GGATG	3 cut(s)	517, 1105, 1219
BstH2I	RGCGCY	1 cut(s)	1006
BstHHI	GCGC	2 cut(s)	25, 1005
BstKTI	GATC	5 cut(s)	93, 721, 1045, 1261, 1292
BstMBI	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 106, 1172
BstNI	CCWGG	6 cut(s)	75, 175, 392, 509, 574, 815
BstNSI	RCATGY	3 cut(s)	138, 190, 1192
BstSCI	CCNGG	7 cut(s)	73, 173, 390, 507, 572, 813, 893
BstSFI	CTRYAG	3 cut(s)	226, 714, 978
BstSLI	GKGCMC	2 cut(s)	1129, 1168
BstV1I	GCAGC	5 cut(s)	212, 322, 325, 799, 964
BstV2I	GAAGAC	2 cut(s)	735, 1191
BstX2I	RGATCY	3 cut(s)	90, 718, 1258
BstXI	CCANNNNNNTGG	2 cut(s)	752, 1175
BstYI	RGATCY	3 cut(s)	90, 718, 1258
Bsu15I	ATCGAT	1 cut(s)	1288
BsuRI	GGCC	7 cut(s)	29, 173, 759, 857, 907, 1084, 1127
BsuTUI	ATCGAT	1 cut(s)	1288
BtgI	CCRYGG	1 cut(s)	477
BtrI	CACGTC	1 cut(s)	733
BtsCI	GGATG	3 cut(s)	517, 1105, 1219
BtsIMutI	CAGTG	2 cut(s)	579, 640
BveI	ACCTGC	1 cut(s)	91
Cac8I	GCNNGC	6 cut(s)	21, 27, 71, 223, 517, 975
CaiI	CAGNNNCTG	2 cut(s)	174, 349
CciI	TCATGA	1 cut(s)	429
CfoI	GCGC	2 cut(s)	25, 1005
Cfr13I	GGNCC	7 cut(s)	28, 171, 505, 667, 758, 1125, 1126
ClaI	ATCGAT	1 cut(s)	1288
CseI	GACGC	2 cut(s)	28, 362
Csp6I	GTAC	4 cut(s)	154, 577, 827, 918
CviAII	CATG	7 cut(s)	135, 187, 248, 430, 478, 1030, 1189
CviQI	GTAC	4 cut(s)	154, 577, 827, 918
DdeI	CTNAG	6 cut(s)	309, 315, 777, 870, 1227, 1281
DpnI	GATC	5 cut(s)	92, 720, 1044, 1260, 1291
DpnII	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
Eam1104I	CTCTTC	3 cut(s)	252, 468, 714
EarI	CTCTTC	3 cut(s)	252, 468, 714
EciI	GGCGGA	1 cut(s)	135
Ecl136II	GAGCTC	1 cut(s)	244
Eco130I	CCWWGG	2 cut(s)	477, 1159
Eco24I	GRGCYC	2 cut(s)	246, 1129
Eco32I	GATATC	2 cut(s)	180, 1278
Eco47I	GGWCC	2 cut(s)	505, 667
Eco47III	AGCGCT	1 cut(s)	1004
Eco53kI	GAGCTC	1 cut(s)	244
Eco57I	CTGAAG	1 cut(s)	1151
Eco88I	CYCGRG	1 cut(s)	94
EcoICRI	GAGCTC	1 cut(s)	244
EcoO109I	RGGNCCY	5 cut(s)	171, 505, 758, 1125, 1126
EcoRII	CCWGG	6 cut(s)	73, 173, 390, 507, 572, 813
EcoRV	GATATC	2 cut(s)	180, 1278
EcoT14I	CCWWGG	2 cut(s)	477, 1159
EcoT38I	GRGCYC	2 cut(s)	246, 1129
ErhI	CCWWGG	2 cut(s)	477, 1159
FaeI	CATG	7 cut(s)	138, 190, 251, 433, 481, 1033, 1192
FalI	AAGNNNNNCTT	4 cut(s)	1124, 1156, 1176, 1208
FatI	CATG	7 cut(s)	134, 186, 247, 429, 477, 1029, 1188
Fnu4HI	GCNGC	6 cut(s)	201, 336, 339, 788, 908, 978
FokI	GGATG	3 cut(s)	524, 1112, 1226
FriOI	GRGCYC	2 cut(s)	246, 1129
Fsp4HI	GCNGC	6 cut(s)	201, 336, 339, 788, 908, 978
FspBI	CTAG	2 cut(s)	537, 1080
GlaI	GCGC	2 cut(s)	24, 1004
GluI	GCNGC	6 cut(s)	201, 336, 339, 788, 908, 978
GsuI	CTGGAG	2 cut(s)	26, 413
HaeII	RGCGCY	1 cut(s)	1006
HaeIII	GGCC	7 cut(s)	29, 173, 759, 857, 907, 1084, 1127
HapII	CCGG	2 cut(s)	674, 895
HgaI	GACGC	2 cut(s)	28, 362
HhaI	GCGC	2 cut(s)	25, 1005
Hin1II	CATG	7 cut(s)	138, 190, 251, 433, 481, 1033, 1192
Hin6I	GCGC	2 cut(s)	23, 1003
HinP1I	GCGC	2 cut(s)	23, 1003
HindIII	AAGCTT	2 cut(s)	123, 219
HinfI	GANTC	4 cut(s)	346, 449, 677, 1058
HpaII	CCGG	2 cut(s)	674, 895
HphI	GGTGA	1 cut(s)	493
Hpy166II	GTNNAC	4 cut(s)	579, 638, 811, 900
Hpy188I	TCNGA	5 cut(s)	310, 605, 996, 1013, 1053
Hpy188III	TCNNGA	8 cut(s)	94, 350, 430, 548, 674, 745, 1181, 1267
Hpy8I	GTNNAC	4 cut(s)	579, 638, 811, 900
Hpy99I	CGWCG	1 cut(s)	1050
HpyAV	CCTTC	4 cut(s)	450, 680, 689, 1264
HpyCH4III	ACNGT	3 cut(s)	282, 386, 554
HpyCH4IV	ACGT	1 cut(s)	732
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 106, 1172
HpyF3I	CTNAG	6 cut(s)	309, 315, 777, 870, 1227, 1281
HpySE526I	ACGT	1 cut(s)	732
Hsp92II	CATG	7 cut(s)	138, 190, 251, 433, 481, 1033, 1192
HspAI	GCGC	2 cut(s)	23, 1003
Kpn2I	TCCGGA	1 cut(s)	673
Kzo9I	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
LmnI	GCTCC	4 cut(s)	66, 148, 249, 1253
Lsp1109I	GCAGC	5 cut(s)	212, 322, 325, 799, 964
LweI	GCATC	6 cut(s)	6, 502, 856, 979, 1090, 1102
MaeI	CTAG	2 cut(s)	537, 1080
MaeII	ACGT	1 cut(s)	732
MaeIII	GTNAC	2 cut(s)	386, 780
MalI	GATC	5 cut(s)	92, 720, 1044, 1260, 1291
MboI	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
MboII	GAAGA	9 cut(s)	269, 281, 455, 538, 701, 740, 875, 1121, 1196
MflI	RGATCY	3 cut(s)	90, 718, 1258
MhlI	GDGCHC	4 cut(s)	71, 246, 1129, 1168
MluCI	AATT	4 cut(s)	273, 357, 700, 884
MlyI	GAGTC	1 cut(s)	686
MroI	TCCGGA	1 cut(s)	673
MseI	TTAA	5 cut(s)	194, 284, 723, 792, 1194
MslI	CAYNNNNRTG	1 cut(s)	584
MspCI	CTTAAG	1 cut(s)	791
MspI	CCGG	2 cut(s)	674, 895
MspR9I	CCNGG	7 cut(s)	75, 175, 392, 509, 574, 815, 895
MvaI	CCWGG	6 cut(s)	75, 175, 392, 509, 574, 815
MwoI	GCNNNNNNNGC	3 cut(s)	10, 106, 1172
NciI	CCSGG	1 cut(s)	895
NcoI	CCATGG	1 cut(s)	477
NdeII	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
NlaIII	CATG	7 cut(s)	138, 190, 251, 433, 481, 1033, 1192
NlaIV	GGNNCC	6 cut(s)	438, 892, 1126, 1127, 1165, 1249
NspI	RCATGY	3 cut(s)	138, 190, 1192
PaeR7I	CTCGAG	1 cut(s)	94
PagI	TCATGA	1 cut(s)	429
PciI	ACATGT	1 cut(s)	134
PfeI	GAWTC	3 cut(s)	346, 449, 1058
PfoI	TCCNGGA	1 cut(s)	507
PkrI	GCNGC	6 cut(s)	202, 337, 340, 789, 909, 979
PleI	GAGTC	1 cut(s)	685
PpsI	GAGTC	1 cut(s)	685
PpuMI	RGGWCCY	1 cut(s)	505
PscI	ACATGT	1 cut(s)	134
PsiI	TTATAA	1 cut(s)	1296
Psp124BI	GAGCTC	1 cut(s)	246
Psp5II	RGGWCCY	1 cut(s)	505
Psp6I	CCWGG	6 cut(s)	73, 173, 390, 507, 572, 813
PspGI	CCWGG	6 cut(s)	73, 173, 390, 507, 572, 813
PspN4I	GGNNCC	6 cut(s)	438, 892, 1126, 1127, 1165, 1249
PspOMI	GGGCCC	1 cut(s)	1125
PspPI	GGNCC	7 cut(s)	28, 171, 505, 667, 758, 1125, 1126
PspPPI	RGGWCCY	1 cut(s)	505
PstI	CTGCAG	1 cut(s)	982
PstNI	CAGNNNCTG	2 cut(s)	174, 349
PsuI	RGATCY	3 cut(s)	90, 718, 1258
RsaI	GTAC	4 cut(s)	155, 578, 828, 919
RsaNI	GTAC	4 cut(s)	154, 577, 827, 918
RseI	CAYNNNNRTG	1 cut(s)	584
SacI	GAGCTC	1 cut(s)	246
SaqAI	TTAA	5 cut(s)	194, 284, 723, 792, 1194
SatI	GCNGC	6 cut(s)	201, 336, 339, 788, 908, 978
Sau3AI	GATC	5 cut(s)	90, 718, 1042, 1258, 1289
Sau96I	GGNCC	7 cut(s)	28, 171, 505, 667, 758, 1125, 1126
ScaI	AGTACT	1 cut(s)	919
SchI	GAGTC	1 cut(s)	686
ScrFI	CCNGG	7 cut(s)	75, 175, 392, 509, 574, 815, 895
SduI	GDGCHC	4 cut(s)	71, 246, 1129, 1168
SfaNI	GCATC	6 cut(s)	6, 502, 856, 979, 1090, 1102
SfcI	CTRYAG	3 cut(s)	226, 714, 978
Sfr274I	CTCGAG	1 cut(s)	94
SinI	GGWCC	2 cut(s)	505, 667
SlaI	CTCGAG	1 cut(s)	94
SmiMI	CAYNNNNRTG	1 cut(s)	584
SmlI	CTYRAG	4 cut(s)	94, 656, 791, 1141
SmoI	CTYRAG	4 cut(s)	94, 656, 791, 1141
Sse9I	AATT	4 cut(s)	273, 357, 700, 884
SsiI	CCGC	2 cut(s)	146, 908
SspMI	CTAG	2 cut(s)	537, 1080
SstI	GAGCTC	1 cut(s)	246
StyD4I	CCNGG	7 cut(s)	73, 173, 390, 507, 572, 813, 893
StyI	CCWWGG	2 cut(s)	477, 1159
TaaI	ACNGT	3 cut(s)	282, 386, 554
TaiI	ACGT	1 cut(s)	735
TaqI	TCGA	2 cut(s)	95, 1288
TaqII	GACCGA	1 cut(s)	997
TasI	AATT	4 cut(s)	273, 357, 700, 884
TatI	WGTACW	1 cut(s)	917
TauI	GCSGC	1 cut(s)	910
TfiI	GAWTC	3 cut(s)	346, 449, 1058
Tru1I	TTAA	5 cut(s)	194, 284, 723, 792, 1194
Tru9I	TTAA	5 cut(s)	194, 284, 723, 792, 1194
TscAI	CASTG	2 cut(s)	586, 640
TseI	GCWGC	5 cut(s)	200, 335, 338, 787, 977
TspDTI	ATGAA	2 cut(s)	1122, 1217
TspRI	CASTG	2 cut(s)	586, 640
Vha464I	CTTAAG	1 cut(s)	791
VpaK11BI	GGWCC	2 cut(s)	505, 667
XapI	RAATTY	1 cut(s)	700
XceI	RCATGY	3 cut(s)	138, 190, 1192
XhoI	CTCGAG	1 cut(s)	94
XspI	CTAG	2 cut(s)	537, 1080
ZrmI	AGTACT	1 cut(s)	919

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.