Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0009143

Physical Location & Seq

Genomic Coordinates Forward (+)

44423347 .. 44430948

Gene Structure 7602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0271800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3375 bp

ATGTCATCTCTGAGCAGAGAGCTGGTTTTCCTGATCCTTCAATTTCTGGAGGAGGAGAAATTCAAGGAGGCTGTACATAGGCTCGAGCAAGAGTCTGGATTCTTCTTCAACATGAAGTACTTCGAGGAGAAAGCGTTGCAGGGAGAGTGGGACGAGGTGGAGAAGTACCTGTCCGGGTTCACCAAGGTGGACGAGAATCGCTACTCTATGAAGATCTTCTTCGAGGTCAGAAAGCAGAAGTATCTGGAAGCGCTGGACAGGCAAGACAGGGCGAAAGCTGTTGAGATACTGGTGAAGGATTTGAAGGTGTTTTCGACGTTTAATGAGGACTTGTACAAGGAAATCACTCATCTGTTGACTCTTGACAACTTCAGAGAAAATGAGCAGTTGTCCAAGTATGGTGATACTAAATCCGCTAGAAGCATAATGCTGGTAGAGCTGAAGAAATTGATAGAAGCAAATCCTCTATTCCGAGAGAAGCTGGTTTTCCCCACTTTGAAGGCGTCACGCTTGCGAACTTTGATCAATCAAAGTTTAAATTGGCAGCACCAGCTATGCAAAAACCCAAGGCCAAATCCTGACATTAAGACCTTATTCATGGACCACTCGTGTTCTCCTCCAAATGGAGCTCGTGCATCGACTCCAGTTACTCTTCCAGTTGCAGCTCTTACAAAGCCTTTAACTTATGCTCCTCTTGGAGCACATGGTGGACCTTTCCCGCCGGCTGCTGCTGCTGCAGCTGCCAATGCGAATGCTTTAGCAGGATGGATGTCCAATTCTAATCCGTCCTTGTCTATGCAATCAGCTCCGGTCTCTGCTTCACCCCTGCCTGTTCAACTTGGTCAAGTTTCTTCTTTAAAGCATCCAAGAACACCATCAACTGGTCTTGGAATGATTGATTATCAAGGCTCTGATCATGAGCAACTAATGAAACGCCTGCGGACTGCACAATCTGTTGATGAGGTTAGCTATCCTCCTCAACATGCTTCCTGGTCGCTAGATGACCTACCGAGAAATGTATCTTGTACACTTCGTCAGGGATCCACTATTATGAGCATGGATTTCCACCCATCTCATCACACATTACTTGCTGTTGGCTGTAGTAATGGAGAAGTTACTCTGTGGGAAGTTGGGTTACGAGAGAAGCTGGTGTCCAAGCCATTCAAAGTATGGGAAATGACTACTTGTTCTCTGACATTTCAGGCGGCTATTGTCAAAGATTCATCAATGTCTGTTAACCGTGTATCATGGAGTCCAGATGGGAACTTAATCGGTGTTGCATTTACGAAACATTTGGTTCATCTGTATGCTTATCAAGGGTCTAATGATCTACGCCAACATTTGGAGATTGATGCCCACAGTGGAGGTGTGAATGACTTGGCATTCTCTCATCCAAACAAGCAATTGTGTGTTGTAACATGTGGAGATGATAAGATGATAAAGGTTTGGGATCTGGCTGGAAGGAAGCTTTTCCACTTTGAAGGTCATGAAGCACCTGTTTACTCGGTTTGCCCCCACCAGAAGGAGAATATTCAGTTTATATTTTCAACTGCTGTTGATGGAAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCAGGGCACTGGTGCACCACAATGCTGTACAGTGATGATGGAAATAGATTGTTCTCTTGTGGGACTAGTAAAGATGGTGACTCCTTCCTGGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACCTATAGTGGGTTCAGAAAGAAATCTAATGGCATCGCTCAATTTGATACCACAAGAAATCACTTTTTGGCTGTTGGTGAAGATAGCCAGATAAAATTTTGGGATATGGATAATACAAATGTTCTTCTCAGTATAGAGGCTGATGGTGGACTACCACCTGTTCCTCGCTTGAGATTCAATAAGGAAGGGAATCTGCTTGTTGTTACCACGGCTGACAATGGATTCAAGGTTCTTGCAAATGCTGATGGTCTTAGATCCTTAAGAGCAATTGAAAATCGATCTTATGAAGCATCAAGAACACCTATTGAGATGAAGGTATCTACTTCGGCCATGGTTCCAAATATCAATCCTGCCATTAATAAAGTAGATCGTATGGATGCAAGCTCTCCTGCCAGACCTACTCAGATTCTTAATGGAGTTGATCCTATGGCAAGAAGTGTAGAAAAGCGAAGACTATTAGATGATGCATCCGAGAAAAATAAACGTTGGGAACTGGCAGAGATTGTTGATCCTGTTCACTGTCGAGTGGCTACGATGCCAGATAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTGTACACAAATTCTGGTGCCGGCATTCTGGCTCTTGGTTCAAATGGGGTCCAGAAGCTATGGAAGTGGAGAAGCAGTGAACAAAATCCAAATGGAAAGGCCACTGCTAGTGTCGTTCCGCAACATTGGCAACCCAACAATGGTCTTTTGATGACTAATGATGTCCCTGAAAACTGTGAGGAAGCAGTTCCATGTATTGCACTCTCAAAGAATGACTCCTATGTGATGTCGGCATGTGGCGGAAAAGTGTCTCTGTTCAATATGATGACATTTAAGGTAATGACAACTTTCATGTCACCTCCACCAGCCTCAACCTTCCTAGCATTTCATCCTCTGGATAATAACATCATAGCAATTGGAACAGAAGATTCAACTATCCACATTTACAATGTTAGAGTTGATGAGGTCAAATCAAAACTGAAAGGCCACCAGAAACACATTACTGGATTAGCGTTTTCTGTTAACCTTAAGATCATGGTTTCATCAGGTGCTGATGCTCAACTATGCTTTTGGAACATGGATACGTGGGAGAAGAGGAAGTCACTGACAATTCAACTGCCAGCCGGAAAGGCGCCTGTTGGTGACACACAAGTGCAATTCTATTCTGATCAAACTCGGTTGTTGGTATGCCATGAGACTCAACTAGCACTATATGATGCTGCCAAAGCAGAATGCATTCGACAGTGGATGCCGCAAGATGTACTGCCTGCACCAATAACATGTGCAGCATATTCCCCCAATAGTCAACTAGTCTATGCGGGTTTTACTGATGGCAATATTGGAGTATTTGATGCTGATAGTCTGAAATTAAGATGTCGTATTTCCATGTCAGTGTACTTGGCACAAGCATCCCCAAGCAGCCAAACTGTGTACCCGCTAGCTCTTACAGCACATCTGCAGGAGCCATATCAGTTCGCCGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAGCCCTCAGACGCTGAGGGGAAGTGGGGAGTTACAGTGCCTGTCGATAACGGAGCACAGAACGGCTGGACAGCAACATCTTCGTCTAACAATCCTGCTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005829 GO:0005911 GO:0006996 GO:0007032 GO:0007033 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0010256 GO:0012505 GO:0016043 GO:0016050 GO:0022603 GO:0030054 GO:0031156 GO:0031285 GO:0031286 GO:0031410 GO:0031982 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032182 GO:0032501 GO:0032502 GO:0036257 GO:0042173 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043621 GO:0043900 GO:0043901 GO:0043937 GO:0043938 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045881 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051241 GO:0055044 GO:0065007 GO:0071840 GO:0075260 GO:0075262 GO:0097708 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ALIX_LYPXL_bnd BRO1

Protein Analysis

1124

Amino Acids

124.64

Weight (kDa)

6.58

Isoelectric Point (pI)

43.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	3.6e-11	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	9.4e-62	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	2.8e-25	TOPLESS, zinc finger domain
Beta-prop_EML_2	PF23414	331 - 467	7.6e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	348 - 563	5.6e-11	WDR3 second beta-propeller domain
Beta-prop_RIG_2nd	PF23775	357 - 499	6.5e-07	RIG second beta-propeller
Beta-prop_WDR3_1st	PF25173	389 - 526	6.2e-14	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	389 - 529	3.3e-06	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	393 - 491	9.3e-09	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	403 - 560	4e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	407 - 623	2.9e-21	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	410 - 663	1.5e-20	THOC3 beta-propeller domain
WDR55	PF24796	411 - 526	5.1e-08	WDR55
Beta-prop_EML	PF23409	448 - 652	8.3e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	449 - 484	1.8e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	457 - 529	3.8e-08	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	464 - 571	2.3e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	465 - 658	3.6e-10	Prp19 WD40 domain
WDR55	PF24796	498 - 658	5e-07	WDR55
Beta-prop_WDR5	PF25175	513 - 661	9e-06	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	519 - 658	3.6e-07	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	816 - 957	2.5e-14	Prp19 WD40 domain
WDR55	PF24796	830 - 947	1.6e-08	WDR55
Beta-prop_WDR3_1st	PF25173	830 - 955	5e-16	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	831 - 956	1.3e-12	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	831 - 954	1e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	832 - 1042	2.7e-18	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	832 - 1040	2e-21	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	833 - 948	9.7e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	836 - 954	1.1e-13	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	849 - 948	6.9e-09	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	853 - 977	9e-12	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	855 - 948	3.4e-08	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	863 - 926	3.3e-06	RIG first beta-propeller
WD40_Prp19	PF24814	880 - 1044	1.2e-16	Prp19 WD40 domain
WD40_Gbeta	PF25391	883 - 1038	1.3e-06	G protein beta WD-40 repeat protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15130
fragaria_vesca	FvH4_4g26260 FvH4_4g26260
malus_domestica	MD13G1031100.v1.1 MD16G1033300.v1.1
prunus_persica	Prupe.1G321000_v2.0.a1 Prupe.1G321000_v2.0.a1
pyrus_communis	pycom13g02720 pycom16g02790
rosa_chinensis	RchiOBHm_Chr4g0433861
rosa_laevigata	RLG00000006722
rosa_multiflora	Rmu_co8294453.1_g000001 Rmu_sc0000352.1_g000001 Rmu_ssc0000164.1_g000001
rosa_roxburghii	Rroxscaffold_5G00375260
rosa_rugosa	Rorug04G0271800 Rorug04G0271900
rosa_samantha	Rh4AG326600 Rh4BG334000 Rh4CG349700 Rh4DG330800
rosa_wichuraiana	Rw4G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	2336, 2890
AccB7I	CCANNNNNTGG	2 cut(s)	881, 1342
AciI	CCGC	9 cut(s)	414, 719, 940, 1205, 2438, 2559, 3011, 3077, 3194
AclI	AACGTT	1 cut(s)	2227
AclWI	GGATC	9 cut(s)	28, 1035, 1048, 1458, 1992, 2159, 2246, 2288, 3262
AcoI	YGGCCR	1 cut(s)	2070
AcsI	RAATTY	3 cut(s)	59, 1838, 2329
AcuI	CTGAAG	2 cut(s)	355, 461
AcyI	GRCGYC	2 cut(s)	503, 2891
AdeI	CACNNNGTG	1 cut(s)	707
AfeI	AGCGCT	1 cut(s)	252
AfiI	CCNNNNNNNGG	6 cut(s)	623, 881, 1342, 1522, 1752, 2459
AflII	CTTAAG	2 cut(s)	2002, 2786
AflIII	ACRYGT	2 cut(s)	1418, 3038
AhlI	ACTAGT	2 cut(s)	1682, 3067
AjnI	CCWGG	4 cut(s)	989, 1598, 1618, 1704
AleI	CACNNNNGTG	2 cut(s)	185, 2909
AloI	GAACNNNNNNTCC	2 cut(s)	1956, 1988
Alw21I	GWGCWC	4 cut(s)	631, 703, 1634, 3328
Alw26I	GTCTC	3 cut(s)	817, 2574, 2948
Alw44I	GTGCAC	1 cut(s)	1630
AlwI	GGATC	9 cut(s)	28, 1035, 1048, 1458, 1992, 2159, 2246, 2288, 3262
AlwNI	CAGNNNCTG	3 cut(s)	2810, 3284, 3311
Ama87I	CYCGRG	1 cut(s)	83
Aor51HI	AGCGCT	1 cut(s)	252
AoxI	GGCC	4 cut(s)	569, 2070, 2418, 2743
ApaLI	GTGCAC	1 cut(s)	1630
ApoI	RAATTY	3 cut(s)	59, 1838, 2329
AseI	ATTAAT	1 cut(s)	2100
Asp700I	GAANNNNTTC	5 cut(s)	119, 215, 1469, 2505, 2994
AspLEI	GCGC	2 cut(s)	253, 2893
AspS9I	GGNCC	4 cut(s)	601, 710, 1743, 2368
AsuC2I	CCSGG	1 cut(s)	175
AsuHPI	GGTGA	8 cut(s)	172, 304, 413, 813, 1706, 1832, 2607, 2912
AsuNHI	GCTAGC	1 cut(s)	3196
AvaI	CYCGRG	1 cut(s)	83
AvaII	GGWCC	4 cut(s)	601, 710, 1743, 2368
BaeGI	GKGCMC	2 cut(s)	1626, 1634
BamHI	GGATCC	1 cut(s)	1040
BanI	GGYRCC	2 cut(s)	2336, 2890
BanII	GRGCYC	3 cut(s)	631, 1598, 3276
BarI	GAAGNNNNNNTAC	2 cut(s)	1119, 1151
BauI	CACGAG	2 cut(s)	607, 630
BbsI	GAAGAC	1 cut(s)	2200
Bbv12I	GWGCWC	4 cut(s)	631, 703, 1634, 3328
BbvCI	CCTCAGC	1 cut(s)	3285
BceAI	ACGGC	3 cut(s)	1968, 3221, 3349
BcgI	CGANNNNNNTGC	2 cut(s)	3333, 3367
BciT130I	CCWGG	4 cut(s)	991, 1600, 1620, 1706
BciVI	GTATCC	1 cut(s)	2833
BclI	TGATCA	3 cut(s)	522, 913, 2926
BcnI	CCSGG	1 cut(s)	175
BcoDI	GTCTC	3 cut(s)	817, 2574, 2948
BcuI	ACTAGT	2 cut(s)	1682, 3067
BfaI	CTAG	8 cut(s)	417, 998, 1683, 2427, 2639, 2963, 3068, 3197
BfmI	CTRYAG	4 cut(s)	735, 1099, 1747, 3215
BfoI	RGCGCY	2 cut(s)	254, 2894
BfrI	CTTAAG	2 cut(s)	2002, 2786
BfuI	GTATCC	1 cut(s)	2833
BglI	GCCNNNNNGGC	1 cut(s)	2888
BglII	AGATCT	1 cut(s)	213
BmcAI	AGTACT	1 cut(s)	119
Bme1390I	CCNGG	5 cut(s)	175, 991, 1600, 1620, 1706
Bme18I	GGWCC	4 cut(s)	601, 710, 1743, 2368
BmeT110I	CYCGRG	1 cut(s)	83
BmgT120I	GGNCC	4 cut(s)	601, 710, 1743, 2368
BmiI	GGNNCC	7 cut(s)	1042, 1597, 2079, 2338, 2369, 2892, 3222
BmrFI	CCNGG	5 cut(s)	175, 991, 1600, 1620, 1706
BmtI	GCTAGC	1 cut(s)	3200
BpiI	GAAGAC	1 cut(s)	2200
BpmI	CTGGAG	4 cut(s)	68, 627, 1582, 1602
Bpu10I	CCTNAGC	1 cut(s)	3285
BpuEI	CTTGAG	1 cut(s)	1933
BpuMI	CCSGG	1 cut(s)	175
Bsa29I	ATCGAT	1 cut(s)	2020
BsaAI	YACGTR	1 cut(s)	2844
BsaHI	GRCGYC	2 cut(s)	503, 2891
BsaI	GGTCTC	1 cut(s)	817
BsaJI	CCNNGG	6 cut(s)	183, 566, 1599, 1619, 1950, 2073
BsaWI	WCCGGW	1 cut(s)	808
BsaXI	ACNNNNNCTCC	8 cut(s)	119, 149, 673, 703, 2380, 2410, 3291, 3321
Bsc4I	CCNNNNNNNGG	6 cut(s)	623, 881, 1342, 1522, 1752, 2459
Bse118I	RCCGGY	2 cut(s)	721, 2339
Bse1I	ACTGG	7 cut(s)	294, 644, 656, 886, 1631, 2241, 2767
BseBI	CCWGG	4 cut(s)	991, 1600, 1620, 1706
BseCI	ATCGAT	1 cut(s)	2020
BseDI	CCNNGG	6 cut(s)	183, 566, 1599, 1619, 1950, 2073
BseGI	GGATG	9 cut(s)	770, 774, 862, 1390, 2125, 2210, 2647, 3012, 3167
BseLI	CCNNNNNNNGG	6 cut(s)	623, 881, 1342, 1522, 1752, 2459
BseMII	CTCAG	4 cut(s)	1885, 2159, 3276, 3291
BseNI	ACTGG	7 cut(s)	294, 644, 656, 886, 1631, 2241, 2767
BseRI	GAGGAG	6 cut(s)	65, 68, 140, 606, 681, 966
BseSI	GKGCMC	2 cut(s)	1626, 1634
BsgI	GTGCAG	3 cut(s)	930, 3012, 3063
BshFI	GGCC	4 cut(s)	571, 2072, 2420, 2745
BshNI	GGYRCC	2 cut(s)	2336, 2890
BshVI	ATCGAT	1 cut(s)	2020
BsiHKAI	GWGCWC	4 cut(s)	631, 703, 1634, 3328
BsiHKCI	CYCGRG	1 cut(s)	83
BsiSI	CCGG	5 cut(s)	174, 722, 809, 2340, 2883
BslFI	GGGAC	3 cut(s)	164, 1693, 2468
BslI	CCNNNNNNNGG	6 cut(s)	623, 881, 1342, 1522, 1752, 2459
BsmAI	GTCTC	3 cut(s)	817, 2574, 2948
BsmFI	GGGAC	3 cut(s)	164, 1693, 2468
BsmI	GAATGC	5 cut(s)	757, 1382, 2343, 2994, 2996
BsnI	GGCC	4 cut(s)	571, 2072, 2420, 2745
Bso31I	GGTCTC	1 cut(s)	817
BsoBI	CYCGRG	1 cut(s)	83
Bsp1286I	GDGCHC	7 cut(s)	631, 703, 1598, 1626, 1634, 3276, 3328
Bsp1407I	TGTACA	5 cut(s)	73, 333, 1025, 1644, 2322
Bsp19I	CCATGG	1 cut(s)	2073
BspACI	CCGC	9 cut(s)	414, 719, 940, 1205, 2438, 2559, 3011, 3077, 3194
BspANI	GGCC	4 cut(s)	571, 2072, 2420, 2745
BspCNI	CTCAG	4 cut(s)	1884, 2158, 3277, 3290
BspDI	ATCGAT	1 cut(s)	2020
BspHI	TCATGA	2 cut(s)	916, 1486
BspLI	GGNNCC	7 cut(s)	1042, 1597, 2079, 2338, 2369, 2892, 3222
BspMAI	CTGCAG	2 cut(s)	739, 3219
BspOI	GCTAGC	1 cut(s)	3200
BspPI	GGATC	9 cut(s)	28, 1035, 1048, 1458, 1992, 2159, 2246, 2288, 3262
BspT107I	GGYRCC	2 cut(s)	2336, 2890
BspTI	CTTAAG	2 cut(s)	2002, 2786
BspTNI	GGTCTC	1 cut(s)	817
BsrFI	RCCGGY	2 cut(s)	721, 2339
BsrGI	TGTACA	5 cut(s)	73, 333, 1025, 1644, 2322
BsrI	ACTGG	7 cut(s)	294, 644, 656, 886, 1631, 2241, 2767
BssAI	RCCGGY	2 cut(s)	721, 2339
BssECI	CCNNGG	6 cut(s)	183, 566, 1599, 1619, 1950, 2073
BssNI	GRCGYC	2 cut(s)	503, 2891
BssSI	CACGAG	2 cut(s)	607, 630
BssT1I	CCWWGG	3 cut(s)	183, 566, 2073
Bst2BI	CACGAG	2 cut(s)	607, 630
Bst2UI	CCWGG	4 cut(s)	991, 1600, 1620, 1706
Bst4CI	ACNGT	8 cut(s)	1241, 1361, 1649, 2264, 2495, 3003, 3187, 3307
Bst6I	CTCTTC	2 cut(s)	657, 2846
BstACI	GRCGYC	2 cut(s)	503, 2891
BstAFI	CTTAAG	2 cut(s)	2002, 2786
BstAUI	TGTACA	5 cut(s)	73, 333, 1025, 1644, 2322
BstBAI	YACGTR	1 cut(s)	2844
BstC8I	GCNNGC	8 cut(s)	512, 723, 938, 2125, 2341, 2880, 3027, 3198
BstDEI	CTNAG	6 cut(s)	11, 1871, 1994, 2145, 3277, 3285
BstDSI	CCRYGG	2 cut(s)	1950, 2073
BstF5I	GGATG	9 cut(s)	770, 774, 862, 1390, 2125, 2210, 2647, 3012, 3167
BstH2I	RGCGCY	2 cut(s)	254, 2894
BstHHI	GCGC	2 cut(s)	253, 2893
BstMAI	GTCTC	3 cut(s)	817, 2574, 2948
BstNI	CCWGG	4 cut(s)	991, 1600, 1620, 1706
BstNSI	RCATGY	4 cut(s)	986, 1422, 2556, 3042
BstSCI	CCNGG	5 cut(s)	173, 989, 1598, 1618, 1704
BstSFI	CTRYAG	4 cut(s)	735, 1099, 1747, 3215
BstSLI	GKGCMC	2 cut(s)	1626, 1634
BstV2I	GAAGAC	1 cut(s)	2200
BstX2I	RGATCY	6 cut(s)	213, 1040, 1450, 1997, 2293, 3254
BstXI	CCANNNNNNTGG	1 cut(s)	1626
BstYI	RGATCY	6 cut(s)	213, 1040, 1450, 1997, 2293, 3254
Bsu15I	ATCGAT	1 cut(s)	2020
BsuI	GTATCC	1 cut(s)	2833
BsuRI	GGCC	4 cut(s)	571, 2072, 2420, 2745
BsuTUI	ATCGAT	1 cut(s)	2020
BtgI	CCRYGG	2 cut(s)	1950, 2073
BtgZI	GCGATG	1 cut(s)	1762
BtsCI	GGATG	9 cut(s)	770, 774, 862, 1390, 2125, 2210, 2647, 3012, 3167
BtsI	GCAGTG	2 cut(s)	2401, 2421
Cac8I	GCNNGC	8 cut(s)	512, 723, 938, 2125, 2341, 2880, 3027, 3198
CaiI	CAGNNNCTG	3 cut(s)	2810, 3284, 3311
CciI	TCATGA	2 cut(s)	916, 1486
CfoI	GCGC	2 cut(s)	253, 2893
Cfr10I	RCCGGY	2 cut(s)	721, 2339
Cfr13I	GGNCC	4 cut(s)	601, 710, 1743, 2368
ClaI	ATCGAT	1 cut(s)	2020
CseI	GACGC	2 cut(s)	492, 3290
DdeI	CTNAG	6 cut(s)	11, 1871, 1994, 2145, 3277, 3285
DinI	GGCGCC	1 cut(s)	2892
DraI	TTTAAA	2 cut(s)	537, 858
DraIII	CACNNNGTG	1 cut(s)	707
EaeI	YGGCCR	1 cut(s)	2070
Eam1104I	CTCTTC	2 cut(s)	657, 2846
EarI	CTCTTC	2 cut(s)	657, 2846
EciI	GGCGGA	1 cut(s)	2574
Ecl136II	GAGCTC	1 cut(s)	629
Eco130I	CCWWGG	3 cut(s)	183, 566, 2073
Eco24I	GRGCYC	3 cut(s)	631, 1598, 3276
Eco31I	GGTCTC	1 cut(s)	817
Eco47I	GGWCC	4 cut(s)	601, 710, 1743, 2368
Eco47III	AGCGCT	1 cut(s)	252
Eco53kI	GAGCTC	1 cut(s)	629
Eco57I	CTGAAG	2 cut(s)	355, 461
Eco88I	CYCGRG	1 cut(s)	83
EcoICRI	GAGCTC	1 cut(s)	629
EcoO109I	RGGNCCY	1 cut(s)	1743
EcoRII	CCWGG	4 cut(s)	989, 1598, 1618, 1704
EcoT14I	CCWWGG	3 cut(s)	183, 566, 2073
EcoT22I	ATGCAT	2 cut(s)	2212, 2996
EcoT38I	GRGCYC	3 cut(s)	631, 1598, 3276
EgeI	GGCGCC	1 cut(s)	2892
EheI	GGCGCC	1 cut(s)	2892
ErhI	CCWWGG	3 cut(s)	183, 566, 2073
FalI	AAGNNNNNCTT	2 cut(s)	203, 235
FaqI	GGGAC	3 cut(s)	164, 1693, 2468
FauI	CCCGC	3 cut(s)	726, 3070, 3201
FbaI	TGATCA	3 cut(s)	522, 913, 2926
FokI	GGATG	9 cut(s)	777, 781, 849, 1377, 2132, 2197, 2634, 3019, 3154
FriOI	GRGCYC	3 cut(s)	631, 1598, 3276
FspBI	CTAG	8 cut(s)	417, 998, 1683, 2427, 2639, 2963, 3068, 3197
GlaI	GCGC	2 cut(s)	252, 2892
GsuI	CTGGAG	4 cut(s)	68, 627, 1582, 1602
HaeII	RGCGCY	2 cut(s)	254, 2894
HaeIII	GGCC	4 cut(s)	571, 2072, 2420, 2745
HapII	CCGG	5 cut(s)	174, 722, 809, 2340, 2883
HgaI	GACGC	2 cut(s)	492, 3290
HhaI	GCGC	2 cut(s)	253, 2893
Hin1I	GRCGYC	2 cut(s)	503, 2891
Hin6I	GCGC	2 cut(s)	251, 2891
HinP1I	GCGC	2 cut(s)	251, 2891
HincII	GTYRAC	5 cut(s)	357, 1237, 1606, 2782, 3065
HindII	GTYRAC	5 cut(s)	357, 1237, 1606, 2782, 3065
HindIII	AAGCTT	2 cut(s)	1466, 1572
HpaI	GTTAAC	2 cut(s)	1237, 2782
HpaII	CCGG	5 cut(s)	174, 722, 809, 2340, 2883
HphI	GGTGA	8 cut(s)	172, 304, 413, 813, 1706, 1832, 2607, 2912
Hpy99I	CGWCG	1 cut(s)	319
HpyCH4III	ACNGT	8 cut(s)	1241, 1361, 1649, 2264, 2495, 3003, 3187, 3307
HpyCH4IV	ACGT	3 cut(s)	317, 2227, 2843
HpyF3I	CTNAG	6 cut(s)	11, 1871, 1994, 2145, 3277, 3285
HpySE526I	ACGT	3 cut(s)	317, 2227, 2843
Hsp92I	GRCGYC	2 cut(s)	503, 2891
HspAI	GCGC	2 cut(s)	251, 2891
KasI	GGCGCC	1 cut(s)	2890
KroI	GCCGGC	2 cut(s)	721, 2339
KroNI	GCCGGC	2 cut(s)	723, 2341
Ksp22I	TGATCA	3 cut(s)	522, 913, 2926
KspAI	GTTAAC	2 cut(s)	1237, 2782
LmnI	GCTCC	8 cut(s)	626, 694, 698, 811, 1601, 1621, 3220, 3323
MaeI	CTAG	8 cut(s)	417, 998, 1683, 2427, 2639, 2963, 3068, 3197
MaeII	ACGT	3 cut(s)	317, 2227, 2843
MfeI	CAATTG	3 cut(s)	1403, 2010, 2673
MflI	RGATCY	6 cut(s)	213, 1040, 1450, 1997, 2293, 3254
MhlI	GDGCHC	7 cut(s)	631, 703, 1598, 1626, 1634, 3276, 3328
Mly113I	GGCGCC	1 cut(s)	2891
MlyI	GAGTC	7 cut(s)	101, 352, 634, 1261, 1691, 2528, 2950
MmeI	TCCRAC	1 cut(s)	3220
Mph1103I	ATGCAT	2 cut(s)	2212, 2996
MroNI	GCCGGC	2 cut(s)	721, 2339
MroXI	GAANNNNTTC	5 cut(s)	119, 215, 1469, 2505, 2994
MslI	CAYNNNNRTG	6 cut(s)	185, 1049, 1305, 1637, 2909, 3149
MspA1I	CMGCKG	1 cut(s)	740
MspCI	CTTAAG	2 cut(s)	2002, 2786
MspI	CCGG	5 cut(s)	174, 722, 809, 2340, 2883
MspR9I	CCNGG	5 cut(s)	175, 991, 1600, 1620, 1706
MunI	CAATTG	3 cut(s)	1403, 2010, 2673
Mva1269I	GAATGC	5 cut(s)	757, 1382, 2343, 2994, 2996
MvaI	CCWGG	4 cut(s)	991, 1600, 1620, 1706
NaeI	GCCGGC	2 cut(s)	723, 2341
NarI	GGCGCC	1 cut(s)	2891
NciI	CCSGG	1 cut(s)	175
NcoI	CCATGG	1 cut(s)	2073
NgoMIV	GCCGGC	2 cut(s)	721, 2339
NheI	GCTAGC	1 cut(s)	3196
NlaIV	GGNNCC	7 cut(s)	1042, 1597, 2079, 2338, 2369, 2892, 3222
NmuCI	GTSAC	5 cut(s)	504, 1694, 2613, 2859, 2900
NsiI	ATGCAT	2 cut(s)	2212, 2996
NspI	RCATGY	4 cut(s)	986, 1422, 2556, 3042
OliI	CACNNNNGTG	2 cut(s)	185, 2909
PaeR7I	CTCGAG	1 cut(s)	83
PagI	TCATGA	2 cut(s)	916, 1486
PciI	ACATGT	2 cut(s)	1418, 3038
PctI	GAATGC	5 cut(s)	757, 1382, 2343, 2994, 2996
PdiI	GCCGGC	2 cut(s)	723, 2341
PdmI	GAANNNNTTC	5 cut(s)	119, 215, 1469, 2505, 2994
PfeI	GAWTC	8 cut(s)	99, 196, 1220, 1917, 1933, 1965, 2149, 2687
PflMI	CCANNNNNTGG	2 cut(s)	881, 1342
PleI	GAGTC	7 cut(s)	100, 352, 634, 1260, 1691, 2528, 2950
PluTI	GGCGCC	1 cut(s)	2894
PpsI	GAGTC	7 cut(s)	100, 352, 634, 1260, 1691, 2528, 2950
Ppu21I	YACGTR	1 cut(s)	2844
PpuMI	RGGWCCY	1 cut(s)	1743
PscI	ACATGT	2 cut(s)	1418, 3038
PshBI	ATTAAT	1 cut(s)	2100
Psp124BI	GAGCTC	1 cut(s)	631
Psp1406I	AACGTT	1 cut(s)	2227
Psp5II	RGGWCCY	1 cut(s)	1743
Psp6I	CCWGG	4 cut(s)	989, 1598, 1618, 1704
PspGI	CCWGG	4 cut(s)	989, 1598, 1618, 1704
PspN4I	GGNNCC	7 cut(s)	1042, 1597, 2079, 2338, 2369, 2892, 3222
PspPI	GGNCC	4 cut(s)	601, 710, 1743, 2368
PspPPI	RGGWCCY	1 cut(s)	1743
PspXI	VCTCGAGB	1 cut(s)	83
PstI	CTGCAG	2 cut(s)	739, 3219
PstNI	CAGNNNCTG	3 cut(s)	2810, 3284, 3311
PsuI	RGATCY	6 cut(s)	213, 1040, 1450, 1997, 2293, 3254
PvuII	CAGCTG	1 cut(s)	740
RseI	CAYNNNNRTG	6 cut(s)	185, 1049, 1305, 1637, 2909, 3149
SacI	GAGCTC	1 cut(s)	631
Sau96I	GGNCC	4 cut(s)	601, 710, 1743, 2368
ScaI	AGTACT	1 cut(s)	119
SchI	GAGTC	7 cut(s)	101, 352, 634, 1261, 1691, 2528, 2950
ScrFI	CCNGG	5 cut(s)	175, 991, 1600, 1620, 1706
SduI	GDGCHC	7 cut(s)	631, 703, 1598, 1626, 1634, 3276, 3328
SfcI	CTRYAG	4 cut(s)	735, 1099, 1747, 3215
SfoI	GGCGCC	1 cut(s)	2892
Sfr274I	CTCGAG	1 cut(s)	83
SinI	GGWCC	4 cut(s)	601, 710, 1743, 2368
SlaI	CTCGAG	1 cut(s)	83
SmiMI	CAYNNNNRTG	6 cut(s)	185, 1049, 1305, 1637, 2909, 3149
SmlI	CTYRAG	4 cut(s)	83, 1912, 2002, 2786
SmoI	CTYRAG	4 cut(s)	83, 1912, 2002, 2786
SpeI	ACTAGT	2 cut(s)	1682, 3067
SsiI	CCGC	9 cut(s)	414, 719, 940, 1205, 2438, 2559, 3011, 3077, 3194
SspDI	GGCGCC	1 cut(s)	2890
SspI	AATATT	2 cut(s)	1531, 3097
SspMI	CTAG	8 cut(s)	417, 998, 1683, 2427, 2639, 2963, 3068, 3197
SstI	GAGCTC	1 cut(s)	631
StyD4I	CCNGG	5 cut(s)	173, 989, 1598, 1618, 1704
StyI	CCWWGG	3 cut(s)	183, 566, 2073
TaaI	ACNGT	8 cut(s)	1241, 1361, 1649, 2264, 2495, 3003, 3187, 3307
TaiI	ACGT	3 cut(s)	320, 2230, 2846
TatI	WGTACW	8 cut(s)	73, 117, 333, 1025, 1644, 2322, 3019, 3153
TauI	GCSGC	2 cut(s)	1208, 3013
TfiI	GAWTC	8 cut(s)	99, 196, 1220, 1917, 1933, 1965, 2149, 2687
TseFI	GTSAC	5 cut(s)	504, 1694, 2613, 2859, 2900
Tsp45I	GTSAC	5 cut(s)	504, 1694, 2613, 2859, 2900
TspGWI	ACGGA	2 cut(s)	774, 3336
Van91I	CCANNNNNTGG	2 cut(s)	881, 1342
Vha464I	CTTAAG	2 cut(s)	2002, 2786
VneI	GTGCAC	1 cut(s)	1630
VpaK11BI	GGWCC	4 cut(s)	601, 710, 1743, 2368
VspI	ATTAAT	1 cut(s)	2100
XapI	RAATTY	3 cut(s)	59, 1838, 2329
XceI	RCATGY	4 cut(s)	986, 1422, 2556, 3042
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1167
XhoI	CTCGAG	1 cut(s)	83
XmnI	GAANNNNTTC	5 cut(s)	119, 215, 1469, 2505, 2994
XspI	CTAG	8 cut(s)	417, 998, 1683, 2427, 2639, 2963, 3068, 3197
ZrmI	AGTACT	1 cut(s)	119
Zsp2I	ATGCAT	2 cut(s)	2212, 2996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.