Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0009143

Physical Location & Seq

Genomic Coordinates Reverse (-)

55040162 .. 55046672

Gene Structure 6511 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G028310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGGCGTCGACGCCGTCCTCCTCCGCCACCACGAACGTCATGCTCGCCATCTTCGAGAAGAAGACCACCCCAATCGATCTCTACCGCCCACTCCGCAACTACATCGCCTTCAATTACTCCGAGCGCGAGGCCCAGAACCTCGAAGACGACCTCCAAACCCTAAAGCAGCTCCGCTCCGACTTGGAGCGCCAACCCGACCCGTCCCTCCCCGCCCGCCGCGACCTCCTCCAAAATTACTTCAAAGCCCTCTGCCTCGTCGAGACCCGCTTCCCAATCTCGCCGGACAAGGACCACATCAACACCGTCACCTTCGTCTGGCACGACGCCTTCAAGAACAAGCAGAAGGCCTCGCAGCAGAACATCCACCTCGAGAAGGCCGCCGTTCTCTTCAACTTGGGCGCCGTCTACAGCCAGATTGGGCTTTCCTACGATCGCGCCACCGTCGACGGCCGCCGCCAGGCCTCCCACGCCTTCATCGCCTCCGCCGGCGCGTTTGCGTTTCTGCGGGACAATGCGGCGACCAAGGCCTCGATTGGGAGCTCCACGACGGTGGATTTGTCGGTGGAGTGCGCCGGGATGCTGGAGAGGTTGATGCTCGCTCAGGCGCAGGAGAGCGTCTTTGAGAATTCCATAGCCAAAGGCAGCACACCTGGTGTTTGTGCCAAGATCTCCAGGCAGGTTGGACTGTACTACGAGGAAGCTTTAGCTGCAACAAATTCTCCCACTTTGAACCAGCATTTTGACAGGGCTTGGATATCACACATGCAGTTAAAGGCCGCACTCTTTTTTGCAGAAGCTTGCTATAGGTATGGTCTAGAACTGCATGAGAAAGAAGAGATTGCAGAAGAAATTGCACGGTTAAAGAGTGGGATAAGTGCTCTAGCGGAGTCTAAGAAATCCACAAAGGGGGCAGCTGCACAAATTTTGGATGCAATTAGCAAGTTAGAGGCCAACCTTAACCGTAACCTTGAGAGGGCTATGAAGGAGAATGATAGAGTTTACCTCATGAGGGTTCCTTCTCCTAGTTCCTTACCGCCCCTTCCAGCATTTTCCATGGTGAAGCCTATGGCGATGAGCGAGGTCTTGGATGCAAGCAAGGAGAAGATGTTTGCTAGTCTTGTTCCGGACAGTAGTGCAAAAGCTCTTTCCAGGTACACTGAAATGGTCGATGATATTATCAGGACGCAAGCTGAAAAATTGCAACAAGCCAGTGAGCTGACCAGAGTTAGGCTAAAGGAAATGGACCTTCCAGAGTCTATTCTTGCTTTGGAAGGAAATTTTACTCTTCCTACGGATCTCAAAGAAGATGTGGAGGCAGTGCAAATTAGTGGGGGCCCTGCAGGTTTGGAAGCTGAGTTGCAGCAACTTAGAGATTTGAGAAGGGTGAACCAGGAAATTCTGGTACATATTGATGAGCTCTTGCAGAAAGAGGCAAGAGAAGATGCCCAGTTTAGAAGCCAATTTGGAACACGGTGGACTAGGCCTCAGTCAAGCACTCTGACTAAGAACTTACAGGATAGGCTAAGTAGGTTTGCAGCTAACTTGAAGCAAGCTTCAGACAGTGATGCTCGGATTGAGCGCTCAGTCAGAGAACATTCAGCTCTCATGTCCATCCTTGATCGTCGCCCGATTGAATCTGCCCTCCCAACTCTGGCTAGGCCAATAATGTCTTTGGATGCCAATGAAGATGCTATTTTAGGGGCCCTGAAGCAAAGCTTGCGGCAATTGGAAACGCTCGGTGCTCAACGAGCAGGCCTTGAAGACATGCTTAAAGAGATGAAAAGAAAGGATGACATACTTCCTAAGTTGATGACATCAACCGGCTCCTATGAGGATCTTTTCAGGAAGGAGATATCAAAATATGATCTTATAACTGAAGACATTGGCCACAATATTGAGGCACAAGAACAACTATTGCTGCAAATCCAGGCCCAAAATGATGAGTTTGCTGCAATCTTCAATCTTGAAGATTACAAAGCATCTCGTGAGAAATGTTACAAGCAGATTCAAGCTGCCATAGCAAAGTACCGTGAAATAAAGGATAACATAAATGAGGGTTTGAAGTTTTATGTTACTCTTCAGGATGCAATAACTAATGTAAAGCAGCAGTGCAGTGACTTTGTGATGACTAGAAATATCCAGTGCCAGGAAATGATTGAAGATGTGCACAAACAAATGGCAGGTCTCAGTTTCCAGGATTCTAAAAGCTCAGGTGCGTACAACAACTATCCTTCTCAAAGTCAAAGATCTGGTTCACAACAGCAAACAGATCCTCGTCCGCAAACACCCTACTACCGACCTCCTGAGCAGCCACAAATGGCTGGTTATGCTCAGCCTCCCCCCTACTCGCAGCAGATGCCACCTCCATACCATGTACCACCTCCTCAAGCTGGTAGTCCATACCCACCCCCTCAAGCTGGTAGTCCTTACCCACCTCCACAGGCCCAGCAACAGCCCCCTGCAGGCCATGAATATGGTCAACCTGCTTATCCAGGGTGGCGGGGTCCATACTACAACGCACAACAATCTGGATCGCATCCTCGACCTCCTTACACCATTCCACCTCAATACCCTCCGCACCAGAGTGGCTACTATAAGCAACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005829 GO:0005911 GO:0006996 GO:0007032 GO:0007033 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0010256 GO:0012505 GO:0016043 GO:0016050 GO:0022603 GO:0030054 GO:0031156 GO:0031285 GO:0031286 GO:0031410 GO:0031982 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032182 GO:0032501 GO:0032502 GO:0036257 GO:0042173 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043621 GO:0043900 GO:0043901 GO:0043937 GO:0043938 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045881 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051241 GO:0055044 GO:0065007 GO:0071840 GO:0075260 GO:0075262 GO:0097708 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ALIX_LYPXL_bnd BRO1

Protein Analysis

868

Amino Acids

97.49

Weight (kDa)

6.11

Isoelectric Point (pI)

62.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRO1	PF03097	14 - 393	1.6e-108	BRO1-like domain
ALIX_LYPXL_bnd	PF13949	428 - 713	8.2e-76	ALIX V-shaped domain binding to HIV

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15130
fragaria_vesca	FvH4_4g26260 FvH4_4g26260
malus_domestica	MD13G1031100.v1.1 MD16G1033300.v1.1
prunus_persica	Prupe.1G321000_v2.0.a1 Prupe.1G321000_v2.0.a1
pyrus_communis	pycom13g02720 pycom16g02790
rosa_chinensis	RchiOBHm_Chr4g0433861
rosa_laevigata	RLG00000006722
rosa_multiflora	Rmu_co8294453.1_g000001 Rmu_sc0000352.1_g000001 Rmu_ssc0000164.1_g000001
rosa_roxburghii	Rroxscaffold_5G00375260
rosa_rugosa	Rorug04G0271800 Rorug04G0271900
rosa_samantha	Rh4AG326600 Rh4BG334000 Rh4CG349700 Rh4DG330800
rosa_wichuraiana	Rw4G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1871
Acc36I	ACCTGC	4 cut(s)	667, 1331, 2171, 2491
AccB1I	GGYRCC	1 cut(s)	398
AccBSI	CCGCTC	1 cut(s)	174
AccI	GTMKAC	3 cut(s)	8, 405, 444
AccII	CGCG	4 cut(s)	126, 219, 435, 491
AccIII	TCCGGA	1 cut(s)	1123
AclWI	GGATC	4 cut(s)	1302, 1842, 2264, 2539
AcoI	YGGCCR	2 cut(s)	448, 1885
AcsI	RAATTY	5 cut(s)	625, 715, 921, 1276, 1395
AcuI	CTGAAG	4 cut(s)	1539, 1727, 1896, 2063
AcyI	GRCGYC	4 cut(s)	5, 11, 324, 399
AdeI	CACNNNGTG	1 cut(s)	653
AfaI	GTAC	6 cut(s)	689, 1154, 1404, 2027, 2219, 2376
AfeI	AGCGCT	1 cut(s)	1580
AjnI	CCWGG	9 cut(s)	456, 649, 671, 1148, 1389, 1926, 2145, 2193, 2491
AleI	CACNNNNGTG	2 cut(s)	548, 2583
Alw21I	GWGCWC	5 cut(s)	542, 880, 1419, 1744, 2169
Alw26I	GTCTC	2 cut(s)	254, 2189
Alw44I	GTGCAC	1 cut(s)	2165
AlwI	GGATC	4 cut(s)	1302, 1842, 2264, 2539
Ama87I	CYCGRG	1 cut(s)	368
Aor13HI	TCCGGA	1 cut(s)	1123
Aor51HI	AGCGCT	1 cut(s)	1580
ApaI	GGGCCC	2 cut(s)	1337, 1705
ApaLI	GTGCAC	1 cut(s)	2165
ApoI	RAATTY	5 cut(s)	625, 715, 921, 1276, 1395
ArsI	GACNNNNNNTTYG	4 cut(s)	538, 570, 2167, 2199
AspLEI	GCGC	8 cut(s)	126, 189, 401, 437, 491, 572, 607, 1581
AsuC2I	CCSGG	1 cut(s)	574
AsuHPI	GGTGA	3 cut(s)	298, 1069, 1396
AvaI	CYCGRG	1 cut(s)	368
AvaII	GGWCC	3 cut(s)	289, 1243, 2504
BaeGI	GKGCMC	3 cut(s)	1337, 1705, 2169
BalI	TGGCCA	1 cut(s)	1887
BanI	GGYRCC	1 cut(s)	398
BanII	GRGCYC	4 cut(s)	542, 1337, 1419, 1705
BauI	CACGAG	1 cut(s)	1983
BbsI	GAAGAC	4 cut(s)	68, 150, 1767, 1884
Bbv12I	GWGCWC	5 cut(s)	542, 880, 1419, 1744, 2169
BccI	CCATC	2 cut(s)	56, 1619
BceAI	ACGGC	3 cut(s)	365, 386, 463
BcgI	CGANNNNNNTGC	4 cut(s)	22, 56, 85, 119
BciT130I	CCWGG	9 cut(s)	458, 651, 673, 1150, 1391, 1928, 2147, 2195, 2493
BcnI	CCSGG	1 cut(s)	574
BcoDI	GTCTC	2 cut(s)	254, 2189
BfaI	CTAG	7 cut(s)	815, 881, 1023, 1113, 1479, 1656, 2130
BfmI	CTRYAG	4 cut(s)	406, 802, 1338, 2460
BfoI	RGCGCY	3 cut(s)	190, 402, 1582
BfuAI	ACCTGC	4 cut(s)	667, 1331, 2171, 2491
BglII	AGATCT	2 cut(s)	666, 2246
BlpI	GCTNAGC	1 cut(s)	2331
Bme18I	GGWCC	3 cut(s)	289, 1243, 2504
BmeT110I	CYCGRG	1 cut(s)	368
BmiI	GGNNCC	8 cut(s)	400, 1014, 1334, 1335, 1702, 1703, 1825, 2505
BmrI	ACTGGG	1 cut(s)	1441
BmuI	ACTGGG	1 cut(s)	1441
BpiI	GAAGAC	4 cut(s)	68, 150, 1767, 1884
BplI	GAGNNNNNCTC	2 cut(s)	987, 1019
BpmI	CTGGAG	2 cut(s)	602, 655
Bpu10I	CCTNAGC	3 cut(s)	600, 2209, 2304
Bpu1102I	GCTNAGC	1 cut(s)	2331
BpuEI	CTTGAG	3 cut(s)	989, 2370, 2397
BpuMI	CCSGG	1 cut(s)	574
Bsa29I	ATCGAT	1 cut(s)	75
BsaHI	GRCGYC	4 cut(s)	5, 11, 324, 399
BsaI	GGTCTC	2 cut(s)	254, 2189
BsaJI	CCNNGG	3 cut(s)	522, 1053, 2492
BsaWI	WCCGGW	1 cut(s)	1123
BsaXI	ACNNNNNCTCC	4 cut(s)	703, 733, 1091, 1121
Bse118I	RCCGGY	2 cut(s)	485, 1820
Bse1I	ACTGG	3 cut(s)	1209, 1447, 2140
BseAI	TCCGGA	1 cut(s)	1123
BseBI	CCWGG	9 cut(s)	458, 651, 673, 1150, 1391, 1928, 2147, 2195, 2493
BseCI	ATCGAT	1 cut(s)	75
BseDI	CCNNGG	3 cut(s)	522, 1053, 2492
BseGI	GGATG	9 cut(s)	360, 582, 934, 1093, 1611, 1681, 1795, 2089, 2536
BseMII	CTCAG	8 cut(s)	614, 1344, 1499, 1596, 2200, 2223, 2295, 2345
BseNI	ACTGG	3 cut(s)	1209, 1447, 2140
BseRI	GAGGAG	3 cut(s)	10, 215, 2373
BseSI	GKGCMC	3 cut(s)	1337, 1705, 2169
BseX3I	CGGCCG	1 cut(s)	448
BseYI	CCCAGC	1 cut(s)	2445
BsgI	GTGCAG	2 cut(s)	900, 2131
Bsh1236I	CGCG	4 cut(s)	126, 219, 435, 491
Bsh1285I	CGRYCG	2 cut(s)	433, 451
BshNI	GGYRCC	1 cut(s)	398
BshVI	ATCGAT	1 cut(s)	75
BsiEI	CGRYCG	2 cut(s)	433, 451
BsiHKAI	GWGCWC	5 cut(s)	542, 880, 1419, 1744, 2169
BsiHKCI	CYCGRG	1 cut(s)	368
BsiSI	CCGG	5 cut(s)	281, 486, 573, 1124, 1821
BslFI	GGGAC	2 cut(s)	187, 521
BsmAI	GTCTC	2 cut(s)	254, 2189
BsmFI	GGGAC	2 cut(s)	187, 521
Bso31I	GGTCTC	2 cut(s)	254, 2189
BsoBI	CYCGRG	1 cut(s)	368
Bsp120I	GGGCCC	2 cut(s)	1333, 1701
Bsp1286I	GDGCHC	7 cut(s)	542, 880, 1337, 1419, 1705, 1744, 2169
Bsp13I	TCCGGA	1 cut(s)	1123
Bsp1720I	GCTNAGC	1 cut(s)	2331
Bsp19I	CCATGG	1 cut(s)	1053
BspCNI	CTCAG	8 cut(s)	613, 1345, 1498, 1595, 2199, 2222, 2296, 2344
BspDI	ATCGAT	1 cut(s)	75
BspEI	TCCGGA	1 cut(s)	1123
BspFNI	CGCG	4 cut(s)	126, 219, 435, 491
BspHI	TCATGA	1 cut(s)	1005
BspLI	GGNNCC	8 cut(s)	400, 1014, 1334, 1335, 1702, 1703, 1825, 2505
BspMAI	CTGCAG	2 cut(s)	1342, 2464
BspMI	ACCTGC	4 cut(s)	667, 1331, 2171, 2491
BspPI	GGATC	4 cut(s)	1302, 1842, 2264, 2539
BspT107I	GGYRCC	1 cut(s)	398
BspTNI	GGTCTC	2 cut(s)	254, 2189
BsrBI	CCGCTC	1 cut(s)	174
BsrFI	RCCGGY	2 cut(s)	485, 1820
BsrI	ACTGG	3 cut(s)	1209, 1447, 2140
BssAI	RCCGGY	2 cut(s)	485, 1820
BssECI	CCNNGG	3 cut(s)	522, 1053, 2492
BssNI	GRCGYC	4 cut(s)	5, 11, 324, 399
BssSI	CACGAG	1 cut(s)	1983
BssT1I	CCWWGG	2 cut(s)	522, 1053
Bst2BI	CACGAG	1 cut(s)	1983
Bst2UI	CCWGG	9 cut(s)	458, 651, 673, 1150, 1391, 1928, 2147, 2195, 2493
Bst6I	CTCTTC	4 cut(s)	392, 828, 1290, 2082
BstACI	GRCGYC	4 cut(s)	5, 11, 324, 399
BstAPI	GCANNNNNTGC	2 cut(s)	1717, 2356
BstDSI	CCRYGG	1 cut(s)	1053
BstENI	CCTNNNNNAGG	1 cut(s)	371
BstF5I	GGATG	9 cut(s)	360, 582, 934, 1093, 1611, 1681, 1795, 2089, 2536
BstFNI	CGCG	4 cut(s)	126, 219, 435, 491
BstH2I	RGCGCY	3 cut(s)	190, 402, 1582
BstHHI	GCGC	8 cut(s)	126, 189, 401, 437, 491, 572, 607, 1581
BstMAI	GTCTC	2 cut(s)	254, 2189
BstMCI	CGRYCG	2 cut(s)	433, 451
BstNI	CCWGG	9 cut(s)	458, 651, 673, 1150, 1391, 1928, 2147, 2195, 2493
BstNSI	RCATGY	2 cut(s)	766, 1768
BstSFI	CTRYAG	4 cut(s)	406, 802, 1338, 2460
BstSLI	GKGCMC	3 cut(s)	1337, 1705, 2169
BstUI	CGCG	4 cut(s)	126, 219, 435, 491
BstV2I	GAAGAC	4 cut(s)	68, 150, 1767, 1884
BstX2I	RGATCY	5 cut(s)	666, 1294, 1834, 2246, 2269
BstXI	CCANNNNNNTGG	2 cut(s)	550, 2474
BstYI	RGATCY	5 cut(s)	666, 1294, 1834, 2246, 2269
BstZI	CGGCCG	1 cut(s)	448
Bsu15I	ATCGAT	1 cut(s)	75
BsuTUI	ATCGAT	1 cut(s)	75
BtgI	CCRYGG	1 cut(s)	1053
BtgZI	GCGATG	3 cut(s)	88, 460, 1085
BtsCI	GGATG	9 cut(s)	360, 582, 934, 1093, 1611, 1681, 1795, 2089, 2536
BtsI	GCAGTG	3 cut(s)	1323, 2114, 2119
BtsIMutI	CAGTG	7 cut(s)	1155, 1216, 1323, 1567, 2114, 2119, 2147
BveI	ACCTGC	4 cut(s)	667, 1331, 2171, 2491
CciI	TCATGA	1 cut(s)	1005
CfoI	GCGC	8 cut(s)	126, 189, 401, 437, 491, 572, 607, 1581
Cfr10I	RCCGGY	2 cut(s)	485, 1820
ClaI	ATCGAT	1 cut(s)	75
CseI	GACGC	4 cut(s)	19, 332, 604, 1192
CsiI	ACCWGGT	1 cut(s)	649
Csp6I	GTAC	6 cut(s)	688, 1153, 1403, 2026, 2218, 2375
CviAII	CATG	9 cut(s)	40, 763, 824, 1006, 1054, 1606, 1765, 2372, 2468
CviQI	GTAC	6 cut(s)	688, 1153, 1403, 2026, 2218, 2375
DinI	GGCGCC	1 cut(s)	400
DraIII	CACNNNGTG	1 cut(s)	653
EaeI	YGGCCR	2 cut(s)	448, 1885
EagI	CGGCCG	1 cut(s)	448
Eam1104I	CTCTTC	4 cut(s)	392, 828, 1290, 2082
EarI	CTCTTC	4 cut(s)	392, 828, 1290, 2082
EciI	GGCGGA	2 cut(s)	13, 472
Ecl136II	GAGCTC	2 cut(s)	540, 1417
EclXI	CGGCCG	1 cut(s)	448
Eco130I	CCWWGG	2 cut(s)	522, 1053
Eco147I	AGGCCT	5 cut(s)	347, 461, 527, 1483, 1755
Eco24I	GRGCYC	4 cut(s)	542, 1337, 1419, 1705
Eco31I	GGTCTC	2 cut(s)	254, 2189
Eco32I	GATATC	2 cut(s)	756, 1854
Eco47I	GGWCC	3 cut(s)	289, 1243, 2504
Eco47III	AGCGCT	1 cut(s)	1580
Eco52I	CGGCCG	1 cut(s)	448
Eco53kI	GAGCTC	2 cut(s)	540, 1417
Eco57I	CTGAAG	4 cut(s)	1539, 1727, 1896, 2063
Eco88I	CYCGRG	1 cut(s)	368
EcoICRI	GAGCTC	2 cut(s)	540, 1417
EcoNI	CCTNNNNNAGG	1 cut(s)	371
EcoO109I	RGGNCCY	4 cut(s)	1333, 1334, 1701, 1702
EcoRI	GAATTC	1 cut(s)	625
EcoRII	CCWGG	9 cut(s)	456, 649, 671, 1148, 1389, 1926, 2145, 2193, 2491
EcoRV	GATATC	2 cut(s)	756, 1854
EcoT14I	CCWWGG	2 cut(s)	522, 1053
EcoT38I	GRGCYC	4 cut(s)	542, 1337, 1419, 1705
EgeI	GGCGCC	1 cut(s)	400
EheI	GGCGCC	1 cut(s)	400
ErhI	CCWWGG	2 cut(s)	522, 1053
FaeI	CATG	9 cut(s)	43, 766, 827, 1009, 1057, 1609, 1768, 2375, 2471
FalI	AAGNNNNNCTT	4 cut(s)	1700, 1732, 1752, 1784
FaqI	GGGAC	2 cut(s)	187, 521
FatI	CATG	9 cut(s)	39, 762, 823, 1005, 1053, 1605, 1764, 2371, 2467
FauI	CCCGC	5 cut(s)	217, 221, 272, 498, 2493
FblI	GTMKAC	3 cut(s)	8, 405, 444
FokI	GGATG	9 cut(s)	347, 589, 941, 1100, 1598, 1688, 1802, 2096, 2523
FriOI	GRGCYC	4 cut(s)	542, 1337, 1419, 1705
FspBI	CTAG	7 cut(s)	815, 881, 1023, 1113, 1479, 1656, 2130
GlaI	GCGC	8 cut(s)	125, 188, 400, 436, 490, 571, 606, 1580
GsaI	CCCAGC	1 cut(s)	2449
GsuI	CTGGAG	2 cut(s)	602, 655
HaeII	RGCGCY	3 cut(s)	190, 402, 1582
HapII	CCGG	5 cut(s)	281, 486, 573, 1124, 1821
HgaI	GACGC	4 cut(s)	19, 332, 604, 1192
HhaI	GCGC	8 cut(s)	126, 189, 401, 437, 491, 572, 607, 1581
Hin1I	GRCGYC	4 cut(s)	5, 11, 324, 399
Hin1II	CATG	9 cut(s)	43, 766, 827, 1009, 1057, 1609, 1768, 2375, 2471
Hin6I	GCGC	8 cut(s)	124, 187, 399, 435, 489, 570, 605, 1579
HinP1I	GCGC	8 cut(s)	124, 187, 399, 435, 489, 570, 605, 1579
HincII	GTYRAC	3 cut(s)	9, 445, 2480
HindII	GTYRAC	3 cut(s)	9, 445, 2480
HindIII	AAGCTT	4 cut(s)	699, 795, 1551, 1714
HinfI	GANTC	5 cut(s)	887, 1253, 1634, 2005, 2198
HpaII	CCGG	5 cut(s)	281, 486, 573, 1124, 1821
HphI	GGTGA	3 cut(s)	298, 1069, 1396
Hpy188I	TCNGA	6 cut(s)	121, 178, 1500, 1558, 1572, 1589
Hpy99I	CGWCG	8 cut(s)	10, 13, 260, 326, 446, 449, 550, 1626
HpyCH4IV	ACGT	1 cut(s)	36
HpySE526I	ACGT	1 cut(s)	36
Hsp92I	GRCGYC	4 cut(s)	5, 11, 324, 399
Hsp92II	CATG	9 cut(s)	43, 766, 827, 1009, 1057, 1609, 1768, 2375, 2471
HspAI	GCGC	8 cut(s)	124, 187, 399, 435, 489, 570, 605, 1579
KasI	GGCGCC	1 cut(s)	398
Kpn2I	TCCGGA	1 cut(s)	1123
KroI	GCCGGC	1 cut(s)	485
KroNI	GCCGGC	1 cut(s)	487
LmnI	GCTCC	6 cut(s)	174, 179, 184, 537, 545, 1829
MabI	ACCWGGT	1 cut(s)	649
MaeI	CTAG	7 cut(s)	815, 881, 1023, 1113, 1479, 1656, 2130
MaeII	ACGT	1 cut(s)	36
MaeIII	GTNAC	5 cut(s)	304, 962, 1994, 2071, 2114
MbiI	CCGCTC	1 cut(s)	174
MfeI	CAATTG	1 cut(s)	1724
MflI	RGATCY	5 cut(s)	666, 1294, 1834, 2246, 2269
MhlI	GDGCHC	7 cut(s)	542, 880, 1337, 1419, 1705, 1744, 2169
MlsI	TGGCCA	1 cut(s)	1887
MluNI	TGGCCA	1 cut(s)	1887
Mly113I	GGCGCC	1 cut(s)	399
MlyI	GAGTC	2 cut(s)	896, 1262
MmeI	TCCRAC	2 cut(s)	201, 661
Mox20I	TGGCCA	1 cut(s)	1887
MreI	CGCCGGCG	1 cut(s)	485
MroI	TCCGGA	1 cut(s)	1123
MroNI	GCCGGC	1 cut(s)	485
MscI	TGGCCA	1 cut(s)	1887
MseI	TTAA	4 cut(s)	770, 860, 957, 1770
MslI	CAYNNNNRTG	5 cut(s)	548, 1160, 1410, 2472, 2583
Msp20I	TGGCCA	1 cut(s)	1887
MspA1I	CMGCKG	1 cut(s)	914
MspI	CCGG	5 cut(s)	281, 486, 573, 1124, 1821
MunI	CAATTG	1 cut(s)	1724
MvaI	CCWGG	9 cut(s)	458, 651, 673, 1150, 1391, 1928, 2147, 2195, 2493
MvnI	CGCG	4 cut(s)	126, 219, 435, 491
NaeI	GCCGGC	1 cut(s)	487
NarI	GGCGCC	1 cut(s)	399
NciI	CCSGG	1 cut(s)	574
NcoI	CCATGG	1 cut(s)	1053
NgoMIV	GCCGGC	1 cut(s)	485
NlaIII	CATG	9 cut(s)	43, 766, 827, 1009, 1057, 1609, 1768, 2375, 2471
NlaIV	GGNNCC	8 cut(s)	400, 1014, 1334, 1335, 1702, 1703, 1825, 2505
NmuCI	GTSAC	2 cut(s)	304, 2114
NspI	RCATGY	2 cut(s)	766, 1768
OliI	CACNNNNGTG	2 cut(s)	548, 2583
PaeR7I	CTCGAG	1 cut(s)	368
PagI	TCATGA	1 cut(s)	1005
PceI	AGGCCT	5 cut(s)	347, 461, 527, 1483, 1755
PcsI	WCGNNNNNNNCGW	2 cut(s)	51, 318
PdiI	GCCGGC	1 cut(s)	487
PfeI	GAWTC	3 cut(s)	1634, 2005, 2198
PflFI	GACNNNGTC	1 cut(s)	13
PfoI	TCCNGGA	1 cut(s)	2193
Ple19I	CGATCG	1 cut(s)	433
PleI	GAGTC	2 cut(s)	895, 1261
PluTI	GGCGCC	1 cut(s)	402
PpsI	GAGTC	2 cut(s)	895, 1261
PsiI	TTATAA	1 cut(s)	1871
Psp124BI	GAGCTC	2 cut(s)	542, 1419
Psp6I	CCWGG	9 cut(s)	456, 649, 671, 1148, 1389, 1926, 2145, 2193, 2491
PspFI	CCCAGC	1 cut(s)	2445
PspGI	CCWGG	9 cut(s)	456, 649, 671, 1148, 1389, 1926, 2145, 2193, 2491
PspN4I	GGNNCC	8 cut(s)	400, 1014, 1334, 1335, 1702, 1703, 1825, 2505
PspOMI	GGGCCC	2 cut(s)	1333, 1701
PstI	CTGCAG	2 cut(s)	1342, 2464
PsuI	RGATCY	5 cut(s)	666, 1294, 1834, 2246, 2269
PsyI	GACNNNGTC	1 cut(s)	13
PvuI	CGATCG	1 cut(s)	433
PvuII	CAGCTG	1 cut(s)	914
RsaI	GTAC	6 cut(s)	689, 1154, 1404, 2027, 2219, 2376
RsaNI	GTAC	6 cut(s)	688, 1153, 1403, 2026, 2218, 2375
RseI	CAYNNNNRTG	5 cut(s)	548, 1160, 1410, 2472, 2583
SacI	GAGCTC	2 cut(s)	542, 1419
SalI	GTCGAC	2 cut(s)	7, 443
SaqAI	TTAA	4 cut(s)	770, 860, 957, 1770
SbfI	CCTGCAGG	2 cut(s)	1342, 2464
SchI	GAGTC	2 cut(s)	896, 1262
SdaI	CCTGCAGG	2 cut(s)	1342, 2464
SduI	GDGCHC	7 cut(s)	542, 880, 1337, 1419, 1705, 1744, 2169
SexAI	ACCWGGT	1 cut(s)	649
SfcI	CTRYAG	4 cut(s)	406, 802, 1338, 2460
SfoI	GGCGCC	1 cut(s)	400
Sfr274I	CTCGAG	1 cut(s)	368
SgrAI	CRCCGGYG	1 cut(s)	485
SgrDI	CGTCGACG	2 cut(s)	7, 443
SinI	GGWCC	3 cut(s)	289, 1243, 2504
SlaI	CTCGAG	1 cut(s)	368
SmiMI	CAYNNNNRTG	5 cut(s)	548, 1160, 1410, 2472, 2583
SmlI	CTYRAG	4 cut(s)	368, 968, 2385, 2412
SmoI	CTYRAG	4 cut(s)	368, 968, 2385, 2412
Sse8387I	CCTGCAGG	2 cut(s)	1342, 2464
SseBI	AGGCCT	5 cut(s)	347, 461, 527, 1483, 1755
SspDI	GGCGCC	1 cut(s)	398
SspI	AATATT	1 cut(s)	1894
SspMI	CTAG	7 cut(s)	815, 881, 1023, 1113, 1479, 1656, 2130
SstI	GAGCTC	2 cut(s)	542, 1419
StuI	AGGCCT	5 cut(s)	347, 461, 527, 1483, 1755
StyI	CCWWGG	2 cut(s)	522, 1053
TaiI	ACGT	1 cut(s)	39
TatI	WGTACW	1 cut(s)	687
TauI	GCSGC	7 cut(s)	219, 380, 453, 456, 518, 779, 1723
TfiI	GAWTC	3 cut(s)	1634, 2005, 2198
Tru1I	TTAA	4 cut(s)	770, 860, 957, 1770
Tru9I	TTAA	4 cut(s)	770, 860, 957, 1770
TscAI	CASTG	7 cut(s)	1162, 1216, 1323, 1567, 2114, 2119, 2147
TseFI	GTSAC	2 cut(s)	304, 2114
Tsp45I	GTSAC	2 cut(s)	304, 2114
TspDTI	ATGAA	5 cut(s)	463, 995, 1698, 1793, 2484
TspGWI	ACGGA	1 cut(s)	1307
TspRI	CASTG	7 cut(s)	1162, 1216, 1323, 1567, 2114, 2119, 2147
Tth111I	GACNNNGTC	1 cut(s)	13
VneI	GTGCAC	1 cut(s)	2165
VpaK11BI	GGWCC	3 cut(s)	289, 1243, 2504
XagI	CCTNNNNNAGG	1 cut(s)	371
XapI	RAATTY	5 cut(s)	625, 715, 921, 1276, 1395
XbaI	TCTAGA	1 cut(s)	814
XceI	RCATGY	2 cut(s)	766, 1768
XhoI	CTCGAG	1 cut(s)	368
XmiI	GTMKAC	3 cut(s)	8, 405, 444
XspI	CTAG	7 cut(s)	815, 881, 1023, 1113, 1479, 1656, 2130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.