Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009143

Physical Location & Seq

Genomic Coordinates Reverse (-)

2233717 .. 2239986

Gene Structure 6270 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1031100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGGCGTCTTCCGCCTCCGGCACCGCTACTAATGTCATGCTCGCCATCTTCGAGAAGAAAACGACCCAAATCGACCTCTACCGCCCTCTTCGCAACTACATAGCCTTCAACTACTCCGAGCGCGAAGCCCAGAACCTAGAAGACGACCTCCAAACCGTCAAACAGCTCCGCTCCGACGTCGAGCGCCAACCCGACCCGTCGCTCACCACCCGCCGCGACCTCCTTCAAAATTACTTCAAAGCCCTCTGCCTCGTCGAGACCCGCTTCCCCATCTCGCCGGACAAGGATCACATCAACACCGTCACCTTCGTCTGGCACGACGCTTTCAAGAACAAGCAGAAGGCTTCCCAGCAGAACATCCACCTGGAAAAGGCTGCCGTTTTGTTCAATTTGGGCGCCGTGTACAGTCAGATTGGGCTCTCCTACGATCGCGCTACCGTCGACGGTCGCCGGCAGGCGTCGCACGCGTTCATTGCGGCGGCTGGGGCGTTCGCGTTTCTGCGGGACAATGCGGCGACCAAGGCGTCGATTGGGAGCTCGACGACGGTGGATTTGTCGGTGGAGTGCGCTGGGATGCTGGAGAGGCTGATGCTGGCGCAGGCCCAAGAGTGCGTCTTTGAGAATACTATTGCCAAAGGGAGCACGCCAGGTGTTTGTGCCAAGATCTCGAGGCAGGTTGGGCTGTATTACGAGGAAGCTTTAGCGACATGTAATGCTCCGCCCTTGAACCAGCATTTTGACAGGGGCTGGATATCACACATGCAGTTAAAGGCAGCTCTCTTTTATGCAGAAACTTGCTATAGGTATGGTCTAGAGCTCCATGAGAAAGAAGAGATTGCAGAAGAAATTGCACGGTTAAAGAGTGGGATTAGTGCTTTGTCTGAGGCTAAGAAAAACACAAAAGGGGCTGCTGCACAGATTCTGGACGCAATTAGTAAGTTAGATGTCCATTTGAACCGTAACCTGGAGAGGGCTGTAAAGGAGAATGATAGAGTTTATCTCATGAGGGTTCCTTCCCCTGGTTCCTTACCACCTCTTCCGGCATTTTCCATGGTGAAGCCTTTGGCAATGAGTGAGGTCCTGGATGCGAGCAAGGAGAAGATGTTTGCTAGTCTTGTTCCAGACAGTAGTGCAAAAGCTCTTTCCAGGTACACTGAAATGGTTGATGACATTATCAGAACACAAGCAGAAAAATTACAACAAGCCAGTGAACTGACCAGAGTAAGGCTCAAGGAAATGGACCTTCCAGAGTCTATTCTTGCATTGGAAGGGAATTTTACTCTTCCTACAGATCTTAAAGAAGATGTGGAGGCAGTGCAGATATGTGGAGGCCCTGCTGGTTTGGAAGCTGAGTTACAGCAGCTTAAGGATTTGAAAAGGGTAAACCAGGAAATTCTGGTACAGATTGAGGAACTCTTGCAGAAAGAGGCCAGAGAAGATGCTCAGTTTAGAAGCCAATTTGGAACCCGGTGGACGAGGCCGCAGTCAAGTACTTTGACAAAGAACTTACAGGATAGGCTAAATAGGTTTGCAGGGAACATGAAGCAAGCTGCAGATAGTGATGCTCGAATTGAGCGCTCGGTCAGAGAACATTTAGCTCTCATGTCCATCCTTGATCGTCGTCCGATTGAATCTGCCCTCCCAACTCTGGCTAGGCCAATAATGTCTTTGGATGCCAATGAAGATGCTATTTTGGGGGCCCTGAAGCAGAGCTTGAGGCAACTGGAAACCCTTGGTGCTCAACGAGCTGGTCTTGAAGACATGCTTAAAGAGATGAAAAGAAAGGATGATATACTCCCTAAGTTGATGACATCAACTGTCTCCTATGAGGATCTTTTCAGGAAGGAGATATCTAAGTATGATCTCATAAGTGAAGACATTGCCCATAATATTGAGGCACAAGAACAATTATTGCAGCAAATCCAGGCCCAAAATGCTGAGTTTGCTGCAATCTTCAATCTAGAAGATTATAAAGCATCTCGTGAGAAATGTTACAAGCAGATTCAAGCTGCCATAGCAAAGTTCCGCGAAATTAAGGAGAACATAAATGAGGGCTTAAAGTTCTATGTTACTCTTCAGGATGCAATAACGAATGTAAAGCAGCAGTGCAATGACTTTGTGATGACTAGAAACATTCAGTGCAAGGAAATGATTGAAGATGTTCACAAAAAAATGGCAGGCCTCAGTTTCCAGGATACGAAAACCACAGGTGCGTACGACAACAACTATCGTTCTGTGGGACAACCTGGTTCACAACAGCCAACAGATCCTCGTCCTCAAACCCCACCATACTACCATCCTCCTGAGCAGCCAGCAATTGCTGGATATACTCACCCTCCCCCCTATAGCTCAACGCAGCAGATGCCATCTCCTTACCATGTGCCACCACAACCTACAAGTCCATATGCACCTCCTCAAGCTGGTAGTCCATATGCACCTCCTCAAGCTGGTAGTCCATACCCACCTCCTCAAGCTGGTAGTCCATACCCACCTCCACAGACCCAGCAACAGCCACCTGCAAACCATGACTATGGTCAACCTGCTTATCCAGGGTGGCGTGGCCCATACTATAATGCCCATGCACAACAACCTGGATCGCACCCTCGGCCTCCTTACACCATTCCAGGTCAATACCGCCCTCCGCAACAGGGTGGCTACTATAAGCAACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005829 GO:0005911 GO:0006996 GO:0007032 GO:0007033 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0010256 GO:0012505 GO:0016043 GO:0016050 GO:0022603 GO:0030054 GO:0031156 GO:0031285 GO:0031286 GO:0031410 GO:0031982 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032182 GO:0032501 GO:0032502 GO:0036257 GO:0042173 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043621 GO:0043900 GO:0043901 GO:0043937 GO:0043938 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045881 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051241 GO:0055044 GO:0065007 GO:0071840 GO:0075260 GO:0075262 GO:0097708 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ALIX_LYPXL_bnd BRO1

Protein Analysis

891

Amino Acids

99.74

Weight (kDa)

6.34

Isoelectric Point (pI)

59.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRO1	PF03097	13 - 392	2.4e-109	BRO1-like domain
ALIX_LYPXL_bnd	PF13949	427 - 712	2.6e-75	ALIX V-shaped domain binding to HIV

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15130
fragaria_vesca	FvH4_4g26260 FvH4_4g26260
malus_domestica	MD13G1031100.v1.1 MD16G1033300.v1.1
prunus_persica	Prupe.1G321000_v2.0.a1 Prupe.1G321000_v2.0.a1
pyrus_communis	pycom13g02720 pycom16g02790
rosa_chinensis	RchiOBHm_Chr4g0433861
rosa_laevigata	RLG00000006722
rosa_multiflora	Rmu_co8294453.1_g000001 Rmu_sc0000352.1_g000001 Rmu_ssc0000164.1_g000001
rosa_roxburghii	Rroxscaffold_5G00375260
rosa_rugosa	Rorug04G0271800 Rorug04G0271900
rosa_samantha	Rh4AG326600 Rh4BG334000 Rh4CG349700 Rh4DG330800
rosa_wichuraiana	Rw4G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1971
AarI	CACCTGC	1 cut(s)	2524
AasI	GACNNNNNNGTC	1 cut(s)	523
AatII	GACGTC	1 cut(s)	180
Acc36I	ACCTGC	3 cut(s)	664, 2524, 2548
AccB1I	GGYRCC	2 cut(s)	20, 395
AccBSI	CCGCTC	1 cut(s)	171
AccI	GTMKAC	1 cut(s)	441
AccII	CGCG	6 cut(s)	123, 216, 432, 467, 494, 2028
AclWI	GGATC	4 cut(s)	294, 1839, 2261, 2602
AcsI	RAATTY	2 cut(s)	1273, 1392
AcuI	CTGAAG	2 cut(s)	1724, 2060
AcyI	GRCGYC	5 cut(s)	5, 177, 396, 458, 524
AfaI	GTAC	5 cut(s)	404, 1151, 1401, 1492, 2216
AfeI	AGCGCT	1 cut(s)	1577
AflII	CTTAAG	1 cut(s)	1364
AflIII	ACRYGT	2 cut(s)	465, 707
Alw21I	GWGCWC	4 cut(s)	539, 644, 819, 1741
Alw26I	GTCTC	2 cut(s)	251, 1825
AlwI	GGATC	4 cut(s)	294, 1839, 2261, 2602
AlwNI	CAGNNNCTG	2 cut(s)	747, 922
Ama87I	CYCGRG	1 cut(s)	667
Aor51HI	AGCGCT	1 cut(s)	1577
ApaI	GGGCCC	1 cut(s)	1702
ApoI	RAATTY	2 cut(s)	1273, 1392
ArsI	GACNNNNNNTTYG	2 cut(s)	535, 567
Asp700I	GAANNNNTTC	1 cut(s)	2160
AspLEI	GCGC	7 cut(s)	123, 186, 398, 434, 569, 598, 1578
AspS9I	GGNCC	8 cut(s)	601, 1078, 1240, 1331, 1698, 1699, 1927, 2561
AsuC2I	CCSGG	1 cut(s)	1468
AsuHPI	GGTGA	4 cut(s)	196, 295, 1066, 2324
AvaI	CYCGRG	1 cut(s)	667
AvaII	GGWCC	2 cut(s)	1078, 1240
BaeGI	GKGCMC	1 cut(s)	1702
BanI	GGYRCC	2 cut(s)	20, 395
BanII	GRGCYC	4 cut(s)	420, 539, 819, 1702
BarI	GAAGNNNNNNTAC	2 cut(s)	1338, 1370
BauI	CACGAG	1 cut(s)	1980
BbsI	GAAGAC	3 cut(s)	147, 1764, 1881
Bbv12I	GWGCWC	4 cut(s)	539, 644, 819, 1741
BccI	CCATC	5 cut(s)	53, 278, 1616, 2304, 2374
BceAI	ACGGC	2 cut(s)	362, 383
BciVI	GTATCC	1 cut(s)	2188
BcnI	CCSGG	1 cut(s)	1468
BcoDI	GTCTC	2 cut(s)	251, 1825
BfaI	CTAG	6 cut(s)	137, 812, 1110, 1653, 1961, 2127
BfmI	CTRYAG	4 cut(s)	799, 1287, 1551, 2344
BfoI	RGCGCY	3 cut(s)	187, 399, 1579
BfrI	CTTAAG	1 cut(s)	1364
BfuAI	ACCTGC	3 cut(s)	664, 2524, 2548
BfuI	GTATCC	1 cut(s)	2188
BglII	AGATCT	2 cut(s)	663, 1291
BmcAI	AGTACT	1 cut(s)	1492
Bme18I	GGWCC	2 cut(s)	1078, 1240
BmeT110I	CYCGRG	1 cut(s)	667
BmgT120I	GGNCC	8 cut(s)	601, 1078, 1240, 1331, 1698, 1699, 1927, 2561
BmiI	GGNNCC	7 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700
BoxI	GACNNNNGTC	1 cut(s)	2532
BpiI	GAAGAC	3 cut(s)	147, 1764, 1881
BplI	GAGNNNNNCTC	4 cut(s)	984, 1016, 1212, 1244
BpmI	CTGGAG	2 cut(s)	599, 986
Bpu10I	CCTNAGC	1 cut(s)	2304
BpuEI	CTTGAG	5 cut(s)	1214, 1735, 2400, 2427, 2454
BpuMI	CCSGG	1 cut(s)	1468
BsaHI	GRCGYC	5 cut(s)	5, 177, 396, 458, 524
BsaI	GGTCTC	1 cut(s)	251
BsaJI	CCNNGG	6 cut(s)	519, 1018, 1050, 1732, 2549, 2603
BsaXI	ACNNNNNCTCC	4 cut(s)	1088, 1118, 2320, 2350
Bse118I	RCCGGY	1 cut(s)	450
Bse1I	ACTGG	2 cut(s)	1206, 1728
Bse3DI	GCAATG	4 cut(s)	471, 1074, 1878, 2116
BseDI	CCNNGG	6 cut(s)	519, 1018, 1050, 1732, 2549, 2603
BseGI	GGATG	8 cut(s)	357, 579, 1090, 1608, 1678, 1792, 2086, 2296
BseMI	GCAATG	4 cut(s)	471, 1074, 1878, 2116
BseMII	CTCAG	6 cut(s)	873, 1341, 1457, 1929, 2197, 2295
BseNI	ACTGG	2 cut(s)	1206, 1728
BseRI	GAGGAG	3 cut(s)	2403, 2430, 2457
BseSI	GKGCMC	1 cut(s)	1702
BseYI	CCCAGC	4 cut(s)	348, 482, 569, 2502
BsgI	GTGCAG	2 cut(s)	897, 1337
Bsh1236I	CGCG	6 cut(s)	123, 216, 432, 467, 494, 2028
Bsh1285I	CGRYCG	2 cut(s)	430, 448
BshNI	GGYRCC	2 cut(s)	20, 395
BsiEI	CGRYCG	2 cut(s)	430, 448
BsiHKAI	GWGCWC	4 cut(s)	539, 644, 819, 1741
BsiHKCI	CYCGRG	1 cut(s)	667
BsiSI	CCGG	5 cut(s)	18, 278, 451, 1040, 1468
BsiWI	CGTACG	1 cut(s)	2214
BslFI	GGGAC	2 cut(s)	518, 2253
BsmAI	GTCTC	2 cut(s)	251, 1825
BsmFI	GGGAC	2 cut(s)	518, 2253
Bso31I	GGTCTC	1 cut(s)	251
BsoBI	CYCGRG	1 cut(s)	667
Bsp120I	GGGCCC	1 cut(s)	1698
Bsp1286I	GDGCHC	6 cut(s)	420, 539, 644, 819, 1702, 1741
Bsp1407I	TGTACA	1 cut(s)	402
Bsp143I	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
Bsp19I	CCATGG	1 cut(s)	1050
BspCNI	CTCAG	6 cut(s)	874, 1342, 1456, 1930, 2196, 2296
BspFNI	CGCG	6 cut(s)	123, 216, 432, 467, 494, 2028
BspHI	TCATGA	1 cut(s)	1002
BspLI	GGNNCC	7 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700
BspMAI	CTGCAG	1 cut(s)	1555
BspMI	ACCTGC	3 cut(s)	664, 2524, 2548
BspPI	GGATC	4 cut(s)	294, 1839, 2261, 2602
BspT107I	GGYRCC	2 cut(s)	20, 395
BspTI	CTTAAG	1 cut(s)	1364
BspTNI	GGTCTC	1 cut(s)	251
BsrBI	CCGCTC	1 cut(s)	171
BsrDI	GCAATG	4 cut(s)	471, 1074, 1878, 2116
BsrFI	RCCGGY	1 cut(s)	450
BsrGI	TGTACA	1 cut(s)	402
BsrI	ACTGG	2 cut(s)	1206, 1728
BssAI	RCCGGY	1 cut(s)	450
BssECI	CCNNGG	6 cut(s)	519, 1018, 1050, 1732, 2549, 2603
BssMI	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
BssNI	GRCGYC	5 cut(s)	5, 177, 396, 458, 524
BssSI	CACGAG	1 cut(s)	1980
BssT1I	CCWWGG	3 cut(s)	519, 1050, 1732
Bst2BI	CACGAG	1 cut(s)	1980
Bst6I	CTCTTC	5 cut(s)	93, 825, 1041, 1287, 2079
BstACI	GRCGYC	5 cut(s)	5, 177, 396, 458, 524
BstAFI	CTTAAG	1 cut(s)	1364
BstAPI	GCANNNNNTGC	1 cut(s)	2362
BstAUI	TGTACA	1 cut(s)	402
BstDEI	CTNAG	9 cut(s)	882, 888, 1350, 1443, 1800, 1854, 1938, 2183, 2304
BstDSI	CCRYGG	1 cut(s)	1050
BstENI	CCTNNNNNAGG	1 cut(s)	368
BstF5I	GGATG	8 cut(s)	357, 579, 1090, 1608, 1678, 1792, 2086, 2296
BstFNI	CGCG	6 cut(s)	123, 216, 432, 467, 494, 2028
BstH2I	RGCGCY	3 cut(s)	187, 399, 1579
BstHHI	GCGC	7 cut(s)	123, 186, 398, 434, 569, 598, 1578
BstKTI	GATC	9 cut(s)	289, 430, 666, 1294, 1618, 1834, 1864, 2269, 2597
BstMAI	GTCTC	2 cut(s)	251, 1825
BstMBI	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
BstMCI	CGRYCG	2 cut(s)	430, 448
BstNSI	RCATGY	3 cut(s)	711, 763, 1765
BstPAI	GACNNNNGTC	1 cut(s)	2532
BstSFI	CTRYAG	4 cut(s)	799, 1287, 1551, 2344
BstSLI	GKGCMC	1 cut(s)	1702
BstUI	CGCG	6 cut(s)	123, 216, 432, 467, 494, 2028
BstV2I	GAAGAC	3 cut(s)	147, 1764, 1881
BstX2I	RGATCY	4 cut(s)	663, 1291, 1831, 2266
BstXI	CCANNNNNNTGG	1 cut(s)	2531
BstYI	RGATCY	4 cut(s)	663, 1291, 1831, 2266
BsuI	GTATCC	1 cut(s)	2188
BtgI	CCRYGG	1 cut(s)	1050
BtsCI	GGATG	8 cut(s)	357, 579, 1090, 1608, 1678, 1792, 2086, 2296
BtsI	GCAGTG	2 cut(s)	1320, 2111
BtsIMutI	CAGTG	5 cut(s)	1152, 1213, 1320, 2111, 2144
BveI	ACCTGC	3 cut(s)	664, 2524, 2548
CaiI	CAGNNNCTG	2 cut(s)	747, 922
CciI	TCATGA	1 cut(s)	1002
CfoI	GCGC	7 cut(s)	123, 186, 398, 434, 569, 598, 1578
Cfr10I	RCCGGY	1 cut(s)	450
Cfr13I	GGNCC	8 cut(s)	601, 1078, 1240, 1331, 1698, 1699, 1927, 2561
CseI	GACGC	5 cut(s)	329, 447, 513, 601, 935
CsiI	ACCWGGT	1 cut(s)	2245
Csp6I	GTAC	5 cut(s)	403, 1150, 1400, 1491, 2215
CviQI	GTAC	5 cut(s)	403, 1150, 1400, 1491, 2215
DdeI	CTNAG	9 cut(s)	882, 888, 1350, 1443, 1800, 1854, 1938, 2183, 2304
DinI	GGCGCC	1 cut(s)	397
DpnI	GATC	9 cut(s)	288, 429, 665, 1293, 1617, 1833, 1863, 2268, 2596
DpnII	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
DrdI	GACNNNNNNGTC	1 cut(s)	523
DseDI	GACNNNNNNGTC	1 cut(s)	523
Eam1104I	CTCTTC	5 cut(s)	93, 825, 1041, 1287, 2079
EarI	CTCTTC	5 cut(s)	93, 825, 1041, 1287, 2079
EciI	GGCGGA	1 cut(s)	708
Ecl136II	GAGCTC	2 cut(s)	537, 817
Eco130I	CCWWGG	3 cut(s)	519, 1050, 1732
Eco147I	AGGCCT	1 cut(s)	2181
Eco24I	GRGCYC	4 cut(s)	420, 539, 819, 1702
Eco31I	GGTCTC	1 cut(s)	251
Eco32I	GATATC	2 cut(s)	753, 1851
Eco47I	GGWCC	2 cut(s)	1078, 1240
Eco47III	AGCGCT	1 cut(s)	1577
Eco53kI	GAGCTC	2 cut(s)	537, 817
Eco57I	CTGAAG	2 cut(s)	1724, 2060
Eco88I	CYCGRG	1 cut(s)	667
EcoICRI	GAGCTC	2 cut(s)	537, 817
EcoNI	CCTNNNNNAGG	1 cut(s)	368
EcoO109I	RGGNCCY	4 cut(s)	1078, 1331, 1698, 1699
EcoRV	GATATC	2 cut(s)	753, 1851
EcoT14I	CCWWGG	3 cut(s)	519, 1050, 1732
EcoT38I	GRGCYC	4 cut(s)	420, 539, 819, 1702
EgeI	GGCGCC	1 cut(s)	397
EheI	GGCGCC	1 cut(s)	397
ErhI	CCWWGG	3 cut(s)	519, 1050, 1732
FalI	AAGNNNNNCTT	4 cut(s)	1697, 1729, 1749, 1781
FaqI	GGGAC	2 cut(s)	518, 2253
FauI	CCCGC	3 cut(s)	218, 269, 495
FauNDI	CATATG	2 cut(s)	2404, 2431
FblI	GTMKAC	1 cut(s)	441
FokI	GGATG	8 cut(s)	344, 586, 1097, 1595, 1685, 1799, 2093, 2283
FriOI	GRGCYC	4 cut(s)	420, 539, 819, 1702
FspBI	CTAG	6 cut(s)	137, 812, 1110, 1653, 1961, 2127
GlaI	GCGC	7 cut(s)	122, 185, 397, 433, 568, 597, 1577
GsaI	CCCAGC	4 cut(s)	352, 486, 573, 2506
GsuI	CTGGAG	2 cut(s)	599, 986
HaeII	RGCGCY	3 cut(s)	187, 399, 1579
HapII	CCGG	5 cut(s)	18, 278, 451, 1040, 1468
HgaI	GACGC	5 cut(s)	329, 447, 513, 601, 935
HhaI	GCGC	7 cut(s)	123, 186, 398, 434, 569, 598, 1578
Hin1I	GRCGYC	5 cut(s)	5, 177, 396, 458, 524
Hin6I	GCGC	7 cut(s)	121, 184, 396, 432, 567, 596, 1576
HinP1I	GCGC	7 cut(s)	121, 184, 396, 432, 567, 596, 1576
HincII	GTYRAC	2 cut(s)	442, 2537
HindII	GTYRAC	2 cut(s)	442, 2537
HindIII	AAGCTT	1 cut(s)	696
HinfI	GANTC	4 cut(s)	919, 1250, 1631, 2002
HpaII	CCGG	5 cut(s)	18, 278, 451, 1040, 1468
HphI	GGTGA	4 cut(s)	196, 295, 1066, 2324
Hpy166II	GTNNAC	9 cut(s)	403, 442, 1152, 1211, 1384, 1473, 2164, 2252, 2537
Hpy188I	TCNGA	7 cut(s)	118, 175, 411, 883, 1178, 1586, 1626
Hpy8I	GTNNAC	9 cut(s)	403, 442, 1152, 1211, 1384, 1473, 2164, 2252, 2537
HpyAV	CCTTC	8 cut(s)	115, 233, 316, 334, 1023, 1253, 1262, 1837
HpyCH4IV	ACGT	1 cut(s)	177
HpyF3I	CTNAG	9 cut(s)	882, 888, 1350, 1443, 1800, 1854, 1938, 2183, 2304
HpySE526I	ACGT	1 cut(s)	177
Hsp92I	GRCGYC	5 cut(s)	5, 177, 396, 458, 524
HspAI	GCGC	7 cut(s)	121, 184, 396, 432, 567, 596, 1576
KasI	GGCGCC	1 cut(s)	395
KroI	GCCGGC	1 cut(s)	450
KroNI	GCCGGC	1 cut(s)	452
Kzo9I	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
LmnI	GCTCC	6 cut(s)	171, 176, 534, 639, 721, 822
MabI	ACCWGGT	1 cut(s)	2245
MaeI	CTAG	6 cut(s)	137, 812, 1110, 1653, 1961, 2127
MaeII	ACGT	1 cut(s)	177
MaeIII	GTNAC	5 cut(s)	301, 959, 1353, 1991, 2068
MalI	GATC	9 cut(s)	288, 429, 665, 1293, 1617, 1833, 1863, 2268, 2596
MbiI	CCGCTC	1 cut(s)	171
MboI	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
MfeI	CAATTG	1 cut(s)	2316
MflI	RGATCY	4 cut(s)	663, 1291, 1831, 2266
MhlI	GDGCHC	6 cut(s)	420, 539, 644, 819, 1702, 1741
MluI	ACGCGT	1 cut(s)	465
Mly113I	GGCGCC	1 cut(s)	396
MlyI	GAGTC	1 cut(s)	1259
MmeI	TCCRAC	1 cut(s)	198
MroNI	GCCGGC	1 cut(s)	450
MroXI	GAANNNNTTC	1 cut(s)	2160
MseI	TTAA	7 cut(s)	767, 857, 1296, 1365, 1767, 2034, 2057
MslI	CAYNNNNRTG	2 cut(s)	1157, 2529
MspCI	CTTAAG	1 cut(s)	1364
MspI	CCGG	5 cut(s)	18, 278, 451, 1040, 1468
MunI	CAATTG	1 cut(s)	2316
MvnI	CGCG	6 cut(s)	123, 216, 432, 467, 494, 2028
NaeI	GCCGGC	1 cut(s)	452
NarI	GGCGCC	1 cut(s)	396
NciI	CCSGG	1 cut(s)	1468
NcoI	CCATGG	1 cut(s)	1050
NdeI	CATATG	2 cut(s)	2404, 2431
NdeII	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
NgoMIV	GCCGGC	1 cut(s)	450
NlaIV	GGNNCC	7 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700
NmeAIII	GCCGAG	1 cut(s)	2584
NmuCI	GTSAC	1 cut(s)	301
NspI	RCATGY	3 cut(s)	711, 763, 1765
PaeR7I	CTCGAG	1 cut(s)	667
PagI	TCATGA	1 cut(s)	1002
PaqCI	CACCTGC	1 cut(s)	2524
PceI	AGGCCT	1 cut(s)	2181
PciI	ACATGT	1 cut(s)	707
PcsI	WCGNNNNNNNCGW	2 cut(s)	48, 315
PdiI	GCCGGC	1 cut(s)	452
PdmI	GAANNNNTTC	1 cut(s)	2160
PfeI	GAWTC	3 cut(s)	919, 1631, 2002
Pfl23II	CGTACG	1 cut(s)	2214
PfoI	TCCNGGA	2 cut(s)	1080, 2190
Ple19I	CGATCG	1 cut(s)	430
PleI	GAGTC	1 cut(s)	1258
PluTI	GGCGCC	1 cut(s)	399
PpsI	GAGTC	1 cut(s)	1258
PpuMI	RGGWCCY	1 cut(s)	1078
PscI	ACATGT	1 cut(s)	707
PshAI	GACNNNNGTC	1 cut(s)	2532
PsiI	TTATAA	1 cut(s)	1971
Psp124BI	GAGCTC	2 cut(s)	539, 819
Psp5II	RGGWCCY	1 cut(s)	1078
PspFI	CCCAGC	4 cut(s)	348, 482, 569, 2502
PspLI	CGTACG	1 cut(s)	2214
PspN4I	GGNNCC	7 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700
PspOMI	GGGCCC	1 cut(s)	1698
PspPI	GGNCC	8 cut(s)	601, 1078, 1240, 1331, 1698, 1699, 1927, 2561
PspPPI	RGGWCCY	1 cut(s)	1078
PstI	CTGCAG	1 cut(s)	1555
PstNI	CAGNNNCTG	2 cut(s)	747, 922
PsuI	RGATCY	4 cut(s)	663, 1291, 1831, 2266
PvuI	CGATCG	1 cut(s)	430
RsaI	GTAC	5 cut(s)	404, 1151, 1401, 1492, 2216
RsaNI	GTAC	5 cut(s)	403, 1150, 1400, 1491, 2215
RseI	CAYNNNNRTG	2 cut(s)	1157, 2529
SacI	GAGCTC	2 cut(s)	539, 819
SalI	GTCGAC	1 cut(s)	440
SaqAI	TTAA	7 cut(s)	767, 857, 1296, 1365, 1767, 2034, 2057
Sau3AI	GATC	9 cut(s)	286, 427, 663, 1291, 1615, 1831, 1861, 2266, 2594
Sau96I	GGNCC	8 cut(s)	601, 1078, 1240, 1331, 1698, 1699, 1927, 2561
ScaI	AGTACT	1 cut(s)	1492
SchI	GAGTC	1 cut(s)	1259
SduI	GDGCHC	6 cut(s)	420, 539, 644, 819, 1702, 1741
SexAI	ACCWGGT	1 cut(s)	2245
SfcI	CTRYAG	4 cut(s)	799, 1287, 1551, 2344
SfoI	GGCGCC	1 cut(s)	397
Sfr274I	CTCGAG	1 cut(s)	667
SgrDI	CGTCGACG	1 cut(s)	440
SinI	GGWCC	2 cut(s)	1078, 1240
SlaI	CTCGAG	1 cut(s)	667
SmiMI	CAYNNNNRTG	2 cut(s)	1157, 2529
SmlI	CTYRAG	7 cut(s)	667, 1229, 1364, 1714, 2415, 2442, 2469
SmoI	CTYRAG	7 cut(s)	667, 1229, 1364, 1714, 2415, 2442, 2469
SseBI	AGGCCT	1 cut(s)	2181
SspDI	GGCGCC	1 cut(s)	395
SspI	AATATT	1 cut(s)	1891
SspMI	CTAG	6 cut(s)	137, 812, 1110, 1653, 1961, 2127
SstI	GAGCTC	2 cut(s)	539, 819
StuI	AGGCCT	1 cut(s)	2181
StyI	CCWWGG	3 cut(s)	519, 1050, 1732
TaiI	ACGT	1 cut(s)	180
TaqI	TCGA	9 cut(s)	51, 72, 180, 255, 441, 527, 539, 668, 1567
TaqII	GACCGA	1 cut(s)	1570
TatI	WGTACW	2 cut(s)	402, 1490
TauI	GCSGC	5 cut(s)	216, 479, 482, 515, 1483
TfiI	GAWTC	3 cut(s)	919, 1631, 2002
Tru1I	TTAA	7 cut(s)	767, 857, 1296, 1365, 1767, 2034, 2057
Tru9I	TTAA	7 cut(s)	767, 857, 1296, 1365, 1767, 2034, 2057
TscAI	CASTG	5 cut(s)	1159, 1213, 1320, 2111, 2144
TseFI	GTSAC	1 cut(s)	301
Tsp45I	GTSAC	1 cut(s)	301
TspDTI	ATGAA	4 cut(s)	460, 1556, 1695, 1790
TspRI	CASTG	5 cut(s)	1159, 1213, 1320, 2111, 2144
Vha464I	CTTAAG	1 cut(s)	1364
VpaK11BI	GGWCC	2 cut(s)	1078, 1240
XagI	CCTNNNNNAGG	1 cut(s)	368
XapI	RAATTY	2 cut(s)	1273, 1392
XbaI	TCTAGA	2 cut(s)	811, 1960
XceI	RCATGY	3 cut(s)	711, 763, 1765
XhoI	CTCGAG	1 cut(s)	667
XmiI	GTMKAC	1 cut(s)	441
XmnI	GAANNNNTTC	1 cut(s)	2160
XspI	CTAG	6 cut(s)	137, 812, 1110, 1653, 1961, 2127
ZraI	GACGTC	1 cut(s)	178
ZrmI	AGTACT	1 cut(s)	1492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.