Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009143

Physical Location & Seq

Genomic Coordinates Reverse (-)

2413175 .. 2418712

Gene Structure 5538 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1033300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGGCGTCTTCCGCCTCCGGTACCGCCACCCACGTCATGCTCGCAATCTTCGAGAAGAAGACGACCCAAATCGACCTCTACCGCCCTCTCCGCAATTACATCGCCTTCAACTACTCCGAGCGCGAAGCCCAGAACCTCGAAGACGACCTCCAAACCGTCAAACAGCTTCGCTCCGACGTCGAGCGCCAACCCGACCCATCGCTCACCACCCGCCGCGACCTGCTCCAAAATTACTTTAAAACCCTCTGCCTCGTCGAGACCCGCTTCCCCATCTCGCCGGACAAGGATCACATCAACACCGTCACCTTCGTCTGGCACGACGCCTTCAAGAACAAGCAGAAGGCTTCCCAGCAGAACATCCACCTGGAAAAGGCCGCCGTTTTGTTCAATTTGGGGGCCGTGTACAGTCAGATTGGGCTCTCCTACGATCGCACGACCGTCGATGGTCGACGGCAGGCGTCGCACGCGTTTATTGCGGCGGCTGGGGCATTCGCATTTCTGAGGGACAATGCGGCGACCAAGGCGTCGATTGGGAGCTCCACGACGGTGGATTTGTCGGTGGAGTGCGCTGGGATGCTGGAGAGGCTGATCCTGGCTCAGGCCCAAGAGTGCGTCTTTGAGAATACGATTGCCAAAGGGAGCACGCCGGGTGTTTGCGCCAAGATCTCGAGGCAGGTTGGGCTGTATTACGAGGAAGCTTTAGTGACATGTAATGCTCCGCCCTTGAACCAGCATTTTGACAGGGCCTGGATATCACACATGCAGTTAAAGGCAGCTCTCTTTTATGCAGAAGCTTGCTATAGGTATGGTCTAGAGTTGCATGAGAAAGATGAGATTGCAGAAGAAATTGCACGGTTAAAGAGTGGGATCAGTGCTTTGTCTGAGGCTAAGAAAAACACAAAGGGGGCTGCTGCACAGATTCTGGACGCAATTAGCAAGTTAGAGGTCAATCTGAACCGTAACCTGGAGAGGGCTGTGAAGGAGAATGATAGAGTTTATCTCATGAGGGTTCCTTCCCTTGGTTCCTTACCACCTCTTCCGGCATTTTCCATGGTGAAGCCTTTGGCAATGAGCGAGGTCCTGGATGCGAGCAAGGAGAAGATGTTTGCCAGTCTTGTTCCAGACAGTAGTGCAAAAGCTCTTTCTAGGTACACTGAAATGGTTGATGACATTATCAGAACACAAGCAGAGAAGTTACAACAAGCCAGTGAACTGACCAGAGTAAGGCTTAAGGAAATGGACCTTCCGGAGTCTATTCTTGCCTTGGAAGGGAATTTTACTCTTCCTACAGATCTTAAAGAAGATGTGGAGGCAGTGCAGTTATGTGGAGGTCCTGCTGGTTTGGAAGCTGAGTTACAGCAGCTTAGGGATTTGAAAAGAGTAAACCAGGAAATTTTGGTACATATTGAGGAACTCTTGCAGAAAGAAGCCAGAGAAGATGCTCAGTTTAGAAGCCAATTTGGAACCCGGTGGACGAGGCCGCAGTCAAGTACTCTGACAAAGAACTTACAGGATAGGCTAAATAGGTTTGCAGGCAACTTGAAGCAAGCTGCAGATAGTGATGCTCGGATCGAGCGCTCGGTCCGAGAACATTCAGCTCTCATGTCAATCCTTGATCGTCGTCCGATTGAATCTGCCCTCCCAACTCTGGCTAGGCCAATAATGTCTTTGGATGCCAATGAAGATGCTATTTTGGGGGCCCTGAAGCAGAGCTTGAGGCAACTGGAAACCCTTGGTGCTCAACGAGCTGGTCTTGAAGACATGCTTAAAGAGATGAAAAGAAAGGATGATATACTTCCTAAGTTGATGACGTCAACCGGCTCCTATGAGGATCTTTTCAGGAAGGAGATATCTAAATATGATAATATAAGTGAAGACATTGCCCATAATATTGAGGCACAAGAACAATTATTGCTACAAATCCAGGCCCAAAATGATGAGTTTGCTGCAATCTTCAATCTAGAAGATTATAAAGCATCTCGTGAGAAATGTTACAAGCAGATTCAAGCTGCCATAGCAAAGTTCCGCGAAATTAAGGAGAATATAAATGAGGGCTTAAAGTTCTACGTTACTCTTCAGGATGCAATAACGAATGTAAAGCAGCAGTGCAGTGACTTTGTGATGACTAGAAACATCCAGTGCAAGGAAATGATTGAAGATGTGCACAAACAAATGGCAGGCCTCAGTTTCCAGGATACTAAAACCACAGGTGCATACGACAACAACTATCCTTCTGTGGGACATCAAGCTCAGAGATCTGGTTCACAACAGCCAACAGATCCTCGTTCTCAAACACCACCGTACTACCATCCTCCTGAGCAGCCAGCAATGGCTGGATATTCTCACCCTCCCCCCTATAGCTCAACGCAGCAGATGCCATCTCCTTACATTGTGCCACCTCAACCTACCAGTCCATACGCTCCTCAAGCTGGTAGTCCATACCCACCTCCTCAAGCTGGTAGTCCGTACCCACCTCCACACACCCAGCAACAGCCACCTGCAAACCATGACTATGGTCAACCTGCTTATCCAGGGTGGAGGGGTCCATACTACAATGCCCATGCACAACAACCCGGATCGCACCCTCGACCTCCTTACACCATTCCAGGTCAATACCGTCCTCCGCAACAGGGTGGTTACTATAGCCAACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005829 GO:0005911 GO:0006996 GO:0007032 GO:0007033 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009987 GO:0010256 GO:0012505 GO:0016043 GO:0016050 GO:0022603 GO:0030054 GO:0031156 GO:0031285 GO:0031286 GO:0031410 GO:0031982 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032182 GO:0032501 GO:0032502 GO:0036257 GO:0042173 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043621 GO:0043900 GO:0043901 GO:0043937 GO:0043938 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0045596 GO:0045597 GO:0045881 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051241 GO:0055044 GO:0065007 GO:0071840 GO:0075260 GO:0075262 GO:0097708 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ALIX_LYPXL_bnd BRO1

Protein Analysis

885

Amino Acids

99.16

Weight (kDa)

6.16

Isoelectric Point (pI)

58.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRO1	PF03097	13 - 392	2.1e-108	BRO1-like domain
ALIX_LYPXL_bnd	PF13949	427 - 712	2.3e-76	ALIX V-shaped domain binding to HIV

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15130
fragaria_vesca	FvH4_4g26260 FvH4_4g26260
malus_domestica	MD13G1031100.v1.1 MD16G1033300.v1.1
prunus_persica	Prupe.1G321000_v2.0.a1 Prupe.1G321000_v2.0.a1
pyrus_communis	pycom13g02720 pycom16g02790
rosa_chinensis	RchiOBHm_Chr4g0433861
rosa_laevigata	RLG00000006722
rosa_multiflora	Rmu_co8294453.1_g000001 Rmu_sc0000352.1_g000001 Rmu_ssc0000164.1_g000001
rosa_roxburghii	Rroxscaffold_5G00375260
rosa_rugosa	Rorug04G0271800 Rorug04G0271900
rosa_samantha	Rh4AG326600 Rh4BG334000 Rh4CG349700 Rh4DG330800
rosa_wichuraiana	Rw4G028310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1971
AarI	CACCTGC	1 cut(s)	2506
AasI	GACNNNNNNGTC	1 cut(s)	523
AatII	GACGTC	2 cut(s)	180, 1814
Acc36I	ACCTGC	4 cut(s)	228, 664, 2506, 2530
Acc65I	GGTACC	1 cut(s)	20
AccB1I	GGYRCC	1 cut(s)	20
AccI	GTMKAC	1 cut(s)	448
AccII	CGCG	4 cut(s)	123, 216, 467, 2028
AccIII	TCCGGA	1 cut(s)	1246
AclWI	GGATC	7 cut(s)	294, 583, 875, 1577, 1839, 2273, 2584
AcsI	RAATTY	2 cut(s)	1273, 1392
AcuI	CTGAAG	2 cut(s)	1724, 2060
AcyI	GRCGYC	6 cut(s)	5, 177, 321, 458, 524, 1811
AfaI	GTAC	7 cut(s)	22, 404, 1151, 1401, 1492, 2303, 2468
AfeI	AGCGCT	1 cut(s)	1577
AflII	CTTAAG	1 cut(s)	1229
AflIII	ACRYGT	2 cut(s)	465, 707
AjiI	CACGTC	1 cut(s)	34
AleI	CACNNNNGTG	1 cut(s)	545
Alw21I	GWGCWC	4 cut(s)	539, 644, 1741, 2166
Alw26I	GTCTC	1 cut(s)	251
Alw44I	GTGCAC	1 cut(s)	2162
AlwI	GGATC	7 cut(s)	294, 583, 875, 1577, 1839, 2273, 2584
AlwNI	CAGNNNCTG	2 cut(s)	747, 922
Ama87I	CYCGRG	1 cut(s)	667
Aor13HI	TCCGGA	1 cut(s)	1246
Aor51HI	AGCGCT	1 cut(s)	1577
AoxI	GGCC	9 cut(s)	372, 396, 600, 744, 1478, 1655, 1698, 1926, 2179
ApaI	GGGCCC	1 cut(s)	1702
ApaLI	GTGCAC	1 cut(s)	2162
ApoI	RAATTY	2 cut(s)	1273, 1392
ArsI	GACNNNNNNTTYG	2 cut(s)	535, 567
Asp718I	GGTACC	1 cut(s)	20
AspLEI	GCGC	5 cut(s)	123, 186, 569, 659, 1578
AsuC2I	CCSGG	3 cut(s)	648, 1468, 2574
AsuHPI	GGTGA	4 cut(s)	196, 295, 1066, 2336
AvaI	CYCGRG	1 cut(s)	667
AvaII	GGWCC	5 cut(s)	1078, 1240, 1331, 1582, 2543
BaeGI	GKGCMC	2 cut(s)	1702, 2166
BanI	GGYRCC	1 cut(s)	20
BanII	GRGCYC	3 cut(s)	420, 539, 1702
BarI	GAAGNNNNNNTAC	2 cut(s)	1338, 1370
BauI	CACGAG	1 cut(s)	1980
BbsI	GAAGAC	4 cut(s)	65, 147, 1764, 1881
Bbv12I	GWGCWC	4 cut(s)	539, 644, 1741, 2166
BccI	CCATC	5 cut(s)	205, 278, 437, 2316, 2386
BceAI	ACGGC	3 cut(s)	362, 383, 467
BcgI	CGANNNNNNTGC	4 cut(s)	82, 116, 421, 455
BciVI	GTATCC	1 cut(s)	2188
BcnI	CCSGG	3 cut(s)	648, 1468, 2574
BcoDI	GTCTC	1 cut(s)	251
BfaI	CTAG	5 cut(s)	812, 1146, 1653, 1961, 2127
BfmI	CTRYAG	5 cut(s)	799, 1287, 1551, 2356, 2641
BfoI	RGCGCY	2 cut(s)	187, 1579
BfrI	CTTAAG	1 cut(s)	1229
BfuAI	ACCTGC	4 cut(s)	228, 664, 2506, 2530
BfuI	GTATCC	1 cut(s)	2188
BglII	AGATCT	3 cut(s)	663, 1291, 2255
BmcAI	AGTACT	1 cut(s)	1492
Bme18I	GGWCC	5 cut(s)	1078, 1240, 1331, 1582, 2543
BmeT110I	CYCGRG	1 cut(s)	667
BmgBI	CACGTC	1 cut(s)	34
BmiI	GGNNCC	9 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700, 1822, 2544
BmsI	GCATC	9 cut(s)	564, 1075, 1429, 1552, 1663, 1675, 1985, 2071, 2364
BoxI	GACNNNNGTC	1 cut(s)	2514
BpiI	GAAGAC	4 cut(s)	65, 147, 1764, 1881
BplI	GAGNNNNNCTC	2 cut(s)	984, 1016
BpmI	CTGGAG	2 cut(s)	599, 986
Bpu10I	CCTNAGC	3 cut(s)	597, 1364, 2316
BpuEI	CTTGAG	3 cut(s)	1735, 2409, 2436
BpuMI	CCSGG	3 cut(s)	648, 1468, 2574
BsaHI	GRCGYC	6 cut(s)	5, 177, 321, 458, 524, 1811
BsaI	GGTCTC	1 cut(s)	251
BsaJI	CCNNGG	6 cut(s)	519, 1018, 1050, 1263, 1732, 2531
BsaWI	WCCGGW	2 cut(s)	17, 1246
BsaXI	ACNNNNNCTCC	2 cut(s)	1088, 1118
Bse118I	RCCGGY	1 cut(s)	1817
Bse1I	ACTGG	5 cut(s)	1110, 1206, 1728, 2137, 2409
Bse3DI	GCAATG	3 cut(s)	1074, 1878, 2334
BseAI	TCCGGA	1 cut(s)	1246
BseDI	CCNNGG	6 cut(s)	519, 1018, 1050, 1263, 1732, 2531
BseGI	GGATG	8 cut(s)	357, 579, 1090, 1678, 1792, 2086, 2133, 2308
BseMI	GCAATG	3 cut(s)	1074, 1878, 2334
BseMII	CTCAG	8 cut(s)	491, 611, 873, 1341, 1457, 2197, 2264, 2307
BseNI	ACTGG	5 cut(s)	1110, 1206, 1728, 2137, 2409
BseRI	GAGGAG	2 cut(s)	2412, 2439
BseSI	GKGCMC	2 cut(s)	1702, 2166
BseYI	CCCAGC	4 cut(s)	348, 482, 569, 2484
BsgI	GTGCAG	3 cut(s)	897, 1337, 2128
Bsh1236I	CGCG	4 cut(s)	123, 216, 467, 2028
Bsh1285I	CGRYCG	2 cut(s)	430, 438
BshFI	GGCC	9 cut(s)	374, 398, 602, 746, 1480, 1657, 1700, 1928, 2181
BshNI	GGYRCC	1 cut(s)	20
BsiEI	CGRYCG	2 cut(s)	430, 438
BsiHKAI	GWGCWC	4 cut(s)	539, 644, 1741, 2166
BsiHKCI	CYCGRG	1 cut(s)	667
BsiSI	CCGG	8 cut(s)	18, 278, 647, 1040, 1247, 1468, 1818, 2574
BslFI	GGGAC	2 cut(s)	518, 2253
BsmAI	GTCTC	1 cut(s)	251
BsmFI	GGGAC	2 cut(s)	518, 2253
BsmI	GAATGC	1 cut(s)	488
BsnI	GGCC	9 cut(s)	374, 398, 602, 746, 1480, 1657, 1700, 1928, 2181
Bso31I	GGTCTC	1 cut(s)	251
BsoBI	CYCGRG	1 cut(s)	667
Bsp120I	GGGCCC	1 cut(s)	1698
Bsp1286I	GDGCHC	6 cut(s)	420, 539, 644, 1702, 1741, 2166
Bsp13I	TCCGGA	1 cut(s)	1246
Bsp1407I	TGTACA	1 cut(s)	402
Bsp19I	CCATGG	1 cut(s)	1050
BspANI	GGCC	9 cut(s)	374, 398, 602, 746, 1480, 1657, 1700, 1928, 2181
BspCNI	CTCAG	8 cut(s)	492, 610, 874, 1342, 1456, 2196, 2263, 2308
BspEI	TCCGGA	1 cut(s)	1246
BspFNI	CGCG	4 cut(s)	123, 216, 467, 2028
BspHI	TCATGA	1 cut(s)	1002
BspLI	GGNNCC	9 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700, 1822, 2544
BspMAI	CTGCAG	1 cut(s)	1555
BspMI	ACCTGC	4 cut(s)	228, 664, 2506, 2530
BspPI	GGATC	7 cut(s)	294, 583, 875, 1577, 1839, 2273, 2584
BspT107I	GGYRCC	1 cut(s)	20
BspTI	CTTAAG	1 cut(s)	1229
BspTNI	GGTCTC	1 cut(s)	251
BsrDI	GCAATG	3 cut(s)	1074, 1878, 2334
BsrFI	RCCGGY	1 cut(s)	1817
BsrGI	TGTACA	1 cut(s)	402
BsrI	ACTGG	5 cut(s)	1110, 1206, 1728, 2137, 2409
BssAI	RCCGGY	1 cut(s)	1817
BssECI	CCNNGG	6 cut(s)	519, 1018, 1050, 1263, 1732, 2531
BssNI	GRCGYC	6 cut(s)	5, 177, 321, 458, 524, 1811
BssSI	CACGAG	1 cut(s)	1980
BssT1I	CCWWGG	5 cut(s)	519, 1018, 1050, 1263, 1732
Bst2BI	CACGAG	1 cut(s)	1980
Bst6I	CTCTTC	3 cut(s)	1041, 1287, 2079
BstACI	GRCGYC	6 cut(s)	5, 177, 321, 458, 524, 1811
BstAFI	CTTAAG	1 cut(s)	1229
BstAPI	GCANNNNNTGC	1 cut(s)	2374
BstAUI	TGTACA	1 cut(s)	402
BstDSI	CCRYGG	1 cut(s)	1050
BstENI	CCTNNNNNAGG	2 cut(s)	368, 596
BstF5I	GGATG	8 cut(s)	357, 579, 1090, 1678, 1792, 2086, 2133, 2308
BstFNI	CGCG	4 cut(s)	123, 216, 467, 2028
BstH2I	RGCGCY	2 cut(s)	187, 1579
BstHHI	GCGC	5 cut(s)	123, 186, 569, 659, 1578
BstMAI	GTCTC	1 cut(s)	251
BstMCI	CGRYCG	2 cut(s)	430, 438
BstNSI	RCATGY	3 cut(s)	711, 763, 1765
BstPAI	GACNNNNGTC	1 cut(s)	2514
BstSFI	CTRYAG	5 cut(s)	799, 1287, 1551, 2356, 2641
BstSLI	GKGCMC	2 cut(s)	1702, 2166
BstUI	CGCG	4 cut(s)	123, 216, 467, 2028
BstV2I	GAAGAC	4 cut(s)	65, 147, 1764, 1881
BstX2I	RGATCY	5 cut(s)	663, 1291, 1831, 2255, 2278
BstXI	CCANNNNNNTGG	2 cut(s)	547, 2513
BstYI	RGATCY	5 cut(s)	663, 1291, 1831, 2255, 2278
BsuI	GTATCC	1 cut(s)	2188
BsuRI	GGCC	9 cut(s)	374, 398, 602, 746, 1480, 1657, 1700, 1928, 2181
BtgI	CCRYGG	1 cut(s)	1050
BtgZI	GCGATG	2 cut(s)	85, 183
BtrI	CACGTC	1 cut(s)	34
BtsCI	GGATG	8 cut(s)	357, 579, 1090, 1678, 1792, 2086, 2133, 2308
BtsI	GCAGTG	3 cut(s)	1320, 2111, 2116
BtsIMutI	CAGTG	7 cut(s)	877, 1152, 1213, 1320, 2111, 2116, 2144
BveI	ACCTGC	4 cut(s)	228, 664, 2506, 2530
CaiI	CAGNNNCTG	2 cut(s)	747, 922
CciI	TCATGA	1 cut(s)	1002
CfoI	GCGC	5 cut(s)	123, 186, 569, 659, 1578
Cfr10I	RCCGGY	1 cut(s)	1817
CpoI	CGGWCCG	1 cut(s)	1582
CseI	GACGC	5 cut(s)	329, 447, 513, 601, 935
Csp6I	GTAC	7 cut(s)	21, 403, 1150, 1400, 1491, 2302, 2467
CspI	CGGWCCG	1 cut(s)	1582
CviQI	GTAC	7 cut(s)	21, 403, 1150, 1400, 1491, 2302, 2467
DraI	TTTAAA	1 cut(s)	238
DrdI	GACNNNNNNGTC	1 cut(s)	523
DseDI	GACNNNNNNGTC	1 cut(s)	523
Eam1104I	CTCTTC	3 cut(s)	1041, 1287, 2079
EarI	CTCTTC	3 cut(s)	1041, 1287, 2079
EciI	GGCGGA	1 cut(s)	708
Ecl136II	GAGCTC	1 cut(s)	537
Eco130I	CCWWGG	5 cut(s)	519, 1018, 1050, 1263, 1732
Eco147I	AGGCCT	1 cut(s)	2181
Eco24I	GRGCYC	3 cut(s)	420, 539, 1702
Eco31I	GGTCTC	1 cut(s)	251
Eco32I	GATATC	2 cut(s)	753, 1851
Eco47I	GGWCC	5 cut(s)	1078, 1240, 1331, 1582, 2543
Eco47III	AGCGCT	1 cut(s)	1577
Eco53kI	GAGCTC	1 cut(s)	537
Eco57I	CTGAAG	2 cut(s)	1724, 2060
Eco88I	CYCGRG	1 cut(s)	667
EcoICRI	GAGCTC	1 cut(s)	537
EcoNI	CCTNNNNNAGG	2 cut(s)	368, 596
EcoO109I	RGGNCCY	5 cut(s)	744, 1078, 1331, 1698, 1699
EcoRV	GATATC	2 cut(s)	753, 1851
EcoT14I	CCWWGG	5 cut(s)	519, 1018, 1050, 1263, 1732
EcoT38I	GRGCYC	3 cut(s)	420, 539, 1702
ErhI	CCWWGG	5 cut(s)	519, 1018, 1050, 1263, 1732
FalI	AAGNNNNNCTT	4 cut(s)	1697, 1729, 1749, 1781
FaqI	GGGAC	2 cut(s)	518, 2253
FauI	CCCGC	2 cut(s)	218, 269
FblI	GTMKAC	1 cut(s)	448
FokI	GGATG	8 cut(s)	344, 586, 1097, 1685, 1799, 2093, 2120, 2295
FriOI	GRGCYC	3 cut(s)	420, 539, 1702
FspBI	CTAG	5 cut(s)	812, 1146, 1653, 1961, 2127
GlaI	GCGC	5 cut(s)	122, 185, 568, 658, 1577
GsaI	CCCAGC	4 cut(s)	352, 486, 573, 2488
GsuI	CTGGAG	2 cut(s)	599, 986
HaeII	RGCGCY	2 cut(s)	187, 1579
HaeIII	GGCC	9 cut(s)	374, 398, 602, 746, 1480, 1657, 1700, 1928, 2181
HapII	CCGG	8 cut(s)	18, 278, 647, 1040, 1247, 1468, 1818, 2574
HgaI	GACGC	5 cut(s)	329, 447, 513, 601, 935
HhaI	GCGC	5 cut(s)	123, 186, 569, 659, 1578
Hin1I	GRCGYC	6 cut(s)	5, 177, 321, 458, 524, 1811
Hin6I	GCGC	5 cut(s)	121, 184, 567, 657, 1576
HinP1I	GCGC	5 cut(s)	121, 184, 567, 657, 1576
HincII	GTYRAC	3 cut(s)	449, 1815, 2519
HindII	GTYRAC	3 cut(s)	449, 1815, 2519
HindIII	AAGCTT	2 cut(s)	696, 792
HinfI	GANTC	4 cut(s)	919, 1250, 1631, 2002
HpaII	CCGG	8 cut(s)	18, 278, 647, 1040, 1247, 1468, 1818, 2574
HphI	GGTGA	4 cut(s)	196, 295, 1066, 2336
HpyCH4IV	ACGT	4 cut(s)	33, 177, 1811, 2067
HpySE526I	ACGT	4 cut(s)	33, 177, 1811, 2067
Hsp92I	GRCGYC	6 cut(s)	5, 177, 321, 458, 524, 1811
HspAI	GCGC	5 cut(s)	121, 184, 567, 657, 1576
Kpn2I	TCCGGA	1 cut(s)	1246
KpnI	GGTACC	1 cut(s)	24
LmnI	GCTCC	8 cut(s)	176, 228, 534, 542, 639, 721, 1826, 2425
LweI	GCATC	9 cut(s)	564, 1075, 1429, 1552, 1663, 1675, 1985, 2071, 2364
MaeI	CTAG	5 cut(s)	812, 1146, 1653, 1961, 2127
MaeII	ACGT	4 cut(s)	33, 177, 1811, 2067
MaeIII	GTNAC	9 cut(s)	301, 703, 959, 1194, 1353, 1991, 2068, 2111, 2636
MflI	RGATCY	5 cut(s)	663, 1291, 1831, 2255, 2278
MhlI	GDGCHC	6 cut(s)	420, 539, 644, 1702, 1741, 2166
MluI	ACGCGT	1 cut(s)	465
MlyI	GAGTC	1 cut(s)	1259
MmeI	TCCRAC	1 cut(s)	198
MroI	TCCGGA	1 cut(s)	1246
MseI	TTAA	8 cut(s)	237, 767, 857, 1230, 1296, 1767, 2034, 2057
MslI	CAYNNNNRTG	3 cut(s)	545, 1157, 2511
MspCI	CTTAAG	1 cut(s)	1229
MspI	CCGG	8 cut(s)	18, 278, 647, 1040, 1247, 1468, 1818, 2574
Mva1269I	GAATGC	1 cut(s)	488
MvnI	CGCG	4 cut(s)	123, 216, 467, 2028
NciI	CCSGG	3 cut(s)	648, 1468, 2574
NcoI	CCATGG	1 cut(s)	1050
NlaIV	GGNNCC	9 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700, 1822, 2544
NmuCI	GTSAC	3 cut(s)	301, 703, 2111
NspI	RCATGY	3 cut(s)	711, 763, 1765
OliI	CACNNNNGTG	1 cut(s)	545
PaeR7I	CTCGAG	1 cut(s)	667
PagI	TCATGA	1 cut(s)	1002
PaqCI	CACCTGC	1 cut(s)	2506
PceI	AGGCCT	1 cut(s)	2181
PciI	ACATGT	1 cut(s)	707
PcsI	WCGNNNNNNNCGW	2 cut(s)	48, 315
PctI	GAATGC	1 cut(s)	488
PfeI	GAWTC	3 cut(s)	919, 1631, 2002
PfoI	TCCNGGA	2 cut(s)	1080, 2190
Ple19I	CGATCG	1 cut(s)	430
PleI	GAGTC	1 cut(s)	1258
PpsI	GAGTC	1 cut(s)	1258
PpuMI	RGGWCCY	2 cut(s)	1078, 1331
PscI	ACATGT	1 cut(s)	707
PshAI	GACNNNNGTC	1 cut(s)	2514
PsiI	TTATAA	1 cut(s)	1971
Psp124BI	GAGCTC	1 cut(s)	539
Psp5II	RGGWCCY	2 cut(s)	1078, 1331
PspFI	CCCAGC	4 cut(s)	348, 482, 569, 2484
PspN4I	GGNNCC	9 cut(s)	22, 397, 1011, 1024, 1465, 1699, 1700, 1822, 2544
PspOMI	GGGCCC	1 cut(s)	1698
PspPPI	RGGWCCY	2 cut(s)	1078, 1331
PstI	CTGCAG	1 cut(s)	1555
PstNI	CAGNNNCTG	2 cut(s)	747, 922
PsuI	RGATCY	5 cut(s)	663, 1291, 1831, 2255, 2278
PvuI	CGATCG	1 cut(s)	430
RsaI	GTAC	7 cut(s)	22, 404, 1151, 1401, 1492, 2303, 2468
RsaNI	GTAC	7 cut(s)	21, 403, 1150, 1400, 1491, 2302, 2467
RseI	CAYNNNNRTG	3 cut(s)	545, 1157, 2511
Rsr2I	CGGWCCG	1 cut(s)	1582
RsrII	CGGWCCG	1 cut(s)	1582
SacI	GAGCTC	1 cut(s)	539
SalI	GTCGAC	1 cut(s)	447
SaqAI	TTAA	8 cut(s)	237, 767, 857, 1230, 1296, 1767, 2034, 2057
ScaI	AGTACT	1 cut(s)	1492
SchI	GAGTC	1 cut(s)	1259
SduI	GDGCHC	6 cut(s)	420, 539, 644, 1702, 1741, 2166
SfaNI	GCATC	9 cut(s)	564, 1075, 1429, 1552, 1663, 1675, 1985, 2071, 2364
SfcI	CTRYAG	5 cut(s)	799, 1287, 1551, 2356, 2641
Sfr274I	CTCGAG	1 cut(s)	667
SinI	GGWCC	5 cut(s)	1078, 1240, 1331, 1582, 2543
SlaI	CTCGAG	1 cut(s)	667
SmiMI	CAYNNNNRTG	3 cut(s)	545, 1157, 2511
SmlI	CTYRAG	5 cut(s)	667, 1229, 1714, 2424, 2451
SmoI	CTYRAG	5 cut(s)	667, 1229, 1714, 2424, 2451
SseBI	AGGCCT	1 cut(s)	2181
SspI	AATATT	1 cut(s)	1891
SspMI	CTAG	5 cut(s)	812, 1146, 1653, 1961, 2127
SstI	GAGCTC	1 cut(s)	539
StuI	AGGCCT	1 cut(s)	2181
StyI	CCWWGG	5 cut(s)	519, 1018, 1050, 1263, 1732
TaiI	ACGT	4 cut(s)	36, 180, 1814, 2070
TaqII	GACCGA	1 cut(s)	1570
TatI	WGTACW	2 cut(s)	402, 1490
TauI	GCSGC	6 cut(s)	216, 377, 479, 482, 515, 1483
TfiI	GAWTC	3 cut(s)	919, 1631, 2002
Tru1I	TTAA	8 cut(s)	237, 767, 857, 1230, 1296, 1767, 2034, 2057
Tru9I	TTAA	8 cut(s)	237, 767, 857, 1230, 1296, 1767, 2034, 2057
TscAI	CASTG	7 cut(s)	877, 1159, 1213, 1320, 2111, 2116, 2144
TseFI	GTSAC	3 cut(s)	301, 703, 2111
Tsp45I	GTSAC	3 cut(s)	301, 703, 2111
TspDTI	ATGAA	2 cut(s)	1695, 1790
TspGWI	ACGGA	1 cut(s)	2454
TspRI	CASTG	7 cut(s)	877, 1159, 1213, 1320, 2111, 2116, 2144
Vha464I	CTTAAG	1 cut(s)	1229
VneI	GTGCAC	1 cut(s)	2162
VpaK11BI	GGWCC	5 cut(s)	1078, 1240, 1331, 1582, 2543
XagI	CCTNNNNNAGG	2 cut(s)	368, 596
XapI	RAATTY	2 cut(s)	1273, 1392
XbaI	TCTAGA	2 cut(s)	811, 1960
XceI	RCATGY	3 cut(s)	711, 763, 1765
XhoI	CTCGAG	1 cut(s)	667
XmiI	GTMKAC	1 cut(s)	448
XspI	CTAG	5 cut(s)	812, 1146, 1653, 1961, 2127
ZraI	GACGTC	2 cut(s)	178, 1812
ZrmI	AGTACT	1 cut(s)	1492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.