FvH4_4g26640

Lipid phosphate phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009769
Physical Location & Seq
Reverse (-)
27953739 .. 27957491
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26640.t3

Sequence Viewer

Length: 960 bp
ATGCCGGAGATTCTGTTGGGTTCTCACACCGTAAGGTCTCATGGAGTGCAAGTGGCGAGGTTTCATATGCATGATTGGATCATTCTGGTTGTTCTTGCAGCCATAGATCTTGGTCTGAACTTGATAGAGCCTTTTCACCGATTTGTTGGAGAGGATATGATGACAGACTTGAAATACCCTCTGAAAGACAATACGGTTCCCGTCTGGAGTGTTCCGATAATTGCAATACTGTTACCTCTTGCCATTATTCTTGTTTACTACTTCATCAGAAGGGATGTCTATGATCTACACCATGCCATTTTAGGCCTTCTGTTTTCTGTCTTCATAACCGCTGTTTTAACCGATGCAATCAAAGATGGTGTTGGACGACCTCGTCCAGACTTCTTTTGGCGTTGTTTCCCTGATGGAAAAGGGGTGTTTGATCGTATCACAAAGAATGTCATGTGTACTGGAAAGAAGAGTGTCATTAAGGAAGGACACAAAAGCTTCCCAAGTGGGCATACTTCATGGTCCTTCGCAGGTCTTACTTTCCTTGCATGGTACTTGTCAGGGAAAGTGAGGGTGTTTGATCGTAAGGGGCATGTTGCAAAACTCTGTATTGTTTTTGCCCCATTGCTTCTTGCAGCTATGGTAGGAGTTTCTCGAGTTGATGACTACTGGCATCACTGGCAAGATGTTTTTGCTGGAGGTCTACTAGGAGTCACTGTTTCTTCATTTTGTTACTTGCAATTCTTTCCACCGCCATATGACACTGATGGTTGGGGACCTCATGCGTACTTTCAGATGCTGGCAGACTCTGCAAATGCTCAGCCACTGTCCACCAATGCAAATAATCTTAATGTCCAGCAAGCAGAACTCGAGAGTATATACATCCATCATCAGCAGGTTGTTGAAGCATCACATAGTCAAACCAGTAGTGGAGACACGAACCCCATGCTAAATGGTGGGAGAATACACTAG

Protein Analysis

320

Amino Acids

35.78

Weight (kDa)

6.78

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 104 - 249 2.7e-29 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 372
Acc36I ACCTGC 2 cut(s) 509, 874
AccI GTMKAC 1 cut(s) 691
AciI CCGC 2 cut(s) 330, 740
AclWI GGATC 1 cut(s) 86
AfaI GTAC 3 cut(s) 448, 542, 776
AgsI TTSAA 2 cut(s) 172, 893
AluBI AGCT 2 cut(s) 486, 626
AluI AGCT 2 cut(s) 486, 626
Alw26I GTCTC 2 cut(s) 42, 915
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 3 cut(s) 787, 797, 814
Ama87I CYCGRG 2 cut(s) 642, 857
AoxI GGCC 1 cut(s) 304
ApeKI GCWGC 2 cut(s) 98, 623
AspS9I GGNCC 2 cut(s) 510, 764
AsuHPI GGTGA 1 cut(s) 128
AvaI CYCGRG 2 cut(s) 642, 857
AvaII GGWCC 2 cut(s) 510, 764
BbsI GAAGAC 1 cut(s) 313
BbvI GCAGC 2 cut(s) 110, 635
BccI CCATC 4 cut(s) 350, 398, 749, 882
BcgI CGANNNNNNTGC 2 cut(s) 916, 950
BcoDI GTCTC 2 cut(s) 42, 915
BfaI CTAG 2 cut(s) 695, 958
BfuAI ACCTGC 2 cut(s) 509, 874
BglII AGATCT 1 cut(s) 106
BisI GCNGC 2 cut(s) 99, 624
BlpI GCTNAGC 1 cut(s) 807
BlsI GCNGC 2 cut(s) 100, 625
Bme18I GGWCC 2 cut(s) 510, 764
BmeT110I CYCGRG 2 cut(s) 642, 857
BmgT120I GGNCC 2 cut(s) 510, 764
BmiI GGNNCC 2 cut(s) 198, 765
BmsI GCATC 4 cut(s) 334, 670, 774, 905
BpiI GAAGAC 1 cut(s) 313
BpmI CTGGAG 2 cut(s) 226, 705
Bpu1102I GCTNAGC 1 cut(s) 807
BsaI GGTCTC 1 cut(s) 42
BsaXI ACNNNNNCTCC 5 cut(s) 29, 36, 66, 690, 720
Bse1I ACTGG 4 cut(s) 454, 662, 671, 912
Bse3DI GCAATG 1 cut(s) 611
BseGI GGATG 2 cut(s) 280, 870
BseMI GCAATG 1 cut(s) 611
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 4 cut(s) 454, 662, 671, 912
BseXI GCAGC 2 cut(s) 110, 635
BshFI GGCC 1 cut(s) 306
BsiHKCI CYCGRG 2 cut(s) 642, 857
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 1 cut(s) 777
BsmAI GTCTC 2 cut(s) 42, 915
BsmFI GGGAC 1 cut(s) 777
BsnI GGCC 1 cut(s) 306
Bso31I GGTCTC 1 cut(s) 42
BsoBI CYCGRG 2 cut(s) 642, 857
Bsp143I GATC 5 cut(s) 78, 106, 283, 421, 568
Bsp1720I GCTNAGC 1 cut(s) 807
BspACI CCGC 2 cut(s) 330, 740
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 1 cut(s) 820
BspLI GGNNCC 2 cut(s) 198, 765
BspMI ACCTGC 2 cut(s) 509, 874
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 611
BsrI ACTGG 4 cut(s) 454, 662, 671, 912
BssMI GATC 5 cut(s) 78, 106, 283, 421, 568
Bst4CI ACNGT 5 cut(s) 31, 196, 231, 706, 816
Bst6I CTCTTC 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 797
BstC8I GCNNGC 2 cut(s) 789, 849
BstDEI CTNAG 1 cut(s) 807
BstF5I GGATG 2 cut(s) 280, 870
BstKTI GATC 5 cut(s) 81, 109, 286, 424, 571
BstMAI GTCTC 2 cut(s) 42, 915
BstMBI GATC 5 cut(s) 78, 106, 283, 421, 568
BstMWI GCNNNNNNNGC 2 cut(s) 667, 797
BstNSI RCATGY 1 cut(s) 584
BstV1I GCAGC 2 cut(s) 110, 635
BstV2I GAAGAC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuRI GGCC 1 cut(s) 306
BtsCI GGATG 2 cut(s) 280, 870
BtsIMutI CAGTG 4 cut(s) 664, 702, 750, 812
BveI ACCTGC 2 cut(s) 509, 874
Cac8I GCNNGC 2 cut(s) 789, 849
CaiI CAGNNNCTG 3 cut(s) 787, 797, 814
Cfr13I GGNCC 2 cut(s) 510, 764
Csp6I GTAC 3 cut(s) 447, 541, 775
CviAII CATG 9 cut(s) 41, 71, 293, 442, 507, 537, 581, 770, 934
CviJI RGCY 6 cut(s) 101, 130, 306, 486, 626, 811
CviKI_1 RGCY 6 cut(s) 101, 130, 306, 486, 626, 811
CviQI GTAC 3 cut(s) 447, 541, 775
DdeI CTNAG 1 cut(s) 807
DpnI GATC 5 cut(s) 80, 108, 285, 423, 570
DpnII GATC 5 cut(s) 78, 106, 283, 421, 568
DrdI GACNNNNNNGTC 1 cut(s) 372
DseDI GACNNNNNNGTC 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
Eco147I AGGCCT 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 42
Eco47I GGWCC 2 cut(s) 510, 764
Eco88I CYCGRG 2 cut(s) 642, 857
EcoO109I RGGNCCY 1 cut(s) 764
EcoT22I ATGCAT 1 cut(s) 72
FaeI CATG 9 cut(s) 44, 74, 296, 445, 510, 540, 584, 773, 937
FaqI GGGAC 1 cut(s) 777
FatI CATG 9 cut(s) 40, 70, 292, 441, 506, 536, 580, 769, 933
FauNDI CATATG 2 cut(s) 66, 745
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 2 cut(s) 99, 624
FokI GGATG 2 cut(s) 287, 857
Fsp4HI GCNGC 2 cut(s) 99, 624
FspBI CTAG 2 cut(s) 695, 958
GluI GCNGC 2 cut(s) 99, 624
GsuI CTGGAG 2 cut(s) 226, 705
HaeIII GGCC 1 cut(s) 306
HapII CCGG 1 cut(s) 5
Hin1II CATG 9 cut(s) 44, 74, 296, 445, 510, 540, 584, 773, 937
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 3 cut(s) 10, 699, 794
HpaII CCGG 1 cut(s) 5
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 4 cut(s) 256, 447, 692, 819
Hpy188I TCNGA 5 cut(s) 117, 183, 216, 269, 783
Hpy188III TCNNGA 4 cut(s) 205, 377, 642, 859
Hpy8I GTNNAC 4 cut(s) 256, 447, 692, 819
HpyAV CCTTC 4 cut(s) 264, 317, 467, 523
HpyCH4III ACNGT 5 cut(s) 31, 196, 231, 706, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 667, 797
HpyF3I CTNAG 1 cut(s) 807
Hsp92II CATG 9 cut(s) 44, 74, 296, 445, 510, 540, 584, 773, 937
Kzo9I GATC 5 cut(s) 78, 106, 283, 421, 568
Lsp1109I GCAGC 2 cut(s) 110, 635
LweI GCATC 4 cut(s) 334, 670, 774, 905
MaeI CTAG 2 cut(s) 695, 958
MaeIII GTNAC 3 cut(s) 231, 700, 719
MalI GATC 5 cut(s) 80, 108, 285, 423, 570
MboI GATC 5 cut(s) 78, 106, 283, 421, 568
MboII GAAGA 3 cut(s) 313, 469, 702
MflI RGATCY 1 cut(s) 106
MluCI AATT 2 cut(s) 219, 728
MlyI GAGTC 2 cut(s) 708, 788
MmeI TCCRAC 2 cut(s) 127, 343
MnlI CCTC 8 cut(s) 51, 145, 189, 246, 381, 552, 680, 777
Mph1103I ATGCAT 1 cut(s) 72
MseI TTAA 3 cut(s) 338, 468, 837
MslI CAYNNNNRTG 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 1 cut(s) 5
MwoI GCNNNNNNNGC 2 cut(s) 667, 797
NdeI CATATG 2 cut(s) 66, 745
NdeII GATC 5 cut(s) 78, 106, 283, 421, 568
NlaIII CATG 9 cut(s) 44, 74, 296, 445, 510, 540, 584, 773, 937
NlaIV GGNNCC 2 cut(s) 198, 765
NmuCI GTSAC 1 cut(s) 700
NsiI ATGCAT 1 cut(s) 72
NspI RCATGY 1 cut(s) 584
PaeR7I CTCGAG 2 cut(s) 642, 857
PceI AGGCCT 1 cut(s) 306
PfeI GAWTC 1 cut(s) 10
PflFI GACNNNGTC 1 cut(s) 372
PkrI GCNGC 2 cut(s) 100, 625
PleI GAGTC 2 cut(s) 707, 788
PpsI GAGTC 2 cut(s) 707, 788
PpuMI RGGWCCY 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 764
PspN4I GGNNCC 2 cut(s) 198, 765
PspPI GGNCC 2 cut(s) 510, 764
PspPPI RGGWCCY 1 cut(s) 764
PstNI CAGNNNCTG 3 cut(s) 787, 797, 814
PsuI RGATCY 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 372
RsaI GTAC 3 cut(s) 448, 542, 776
RsaNI GTAC 3 cut(s) 447, 541, 775
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 3 cut(s) 338, 468, 837
SatI GCNGC 2 cut(s) 99, 624
Sau3AI GATC 5 cut(s) 78, 106, 283, 421, 568
Sau96I GGNCC 2 cut(s) 510, 764
SchI GAGTC 2 cut(s) 708, 788
SfaNI GCATC 4 cut(s) 334, 670, 774, 905
Sfr274I CTCGAG 2 cut(s) 642, 857
SinI GGWCC 2 cut(s) 510, 764
SlaI CTCGAG 2 cut(s) 642, 857
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 2 cut(s) 642, 857
SmoI CTYRAG 2 cut(s) 642, 857
Sse9I AATT 2 cut(s) 219, 728
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 2 cut(s) 330, 740
SspMI CTAG 2 cut(s) 695, 958
StuI AGGCCT 1 cut(s) 306
TaaI ACNGT 5 cut(s) 31, 196, 231, 706, 816
TaqI TCGA 2 cut(s) 643, 858
TasI AATT 2 cut(s) 219, 728
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 3 cut(s) 338, 468, 837
Tru9I TTAA 3 cut(s) 338, 468, 837
TscAI CASTG 4 cut(s) 671, 709, 757, 819
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 2 cut(s) 98, 623
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 5 cut(s) 53, 253, 313, 495, 702
TspRI CASTG 4 cut(s) 671, 709, 757, 819
Tth111I GACNNNGTC 1 cut(s) 372
VpaK11BI GGWCC 2 cut(s) 510, 764
XceI RCATGY 1 cut(s) 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 384
XhoI CTCGAG 2 cut(s) 642, 857
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 2 cut(s) 695, 958
Zsp2I ATGCAT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.