pycom16g03150

Lipid phosphate phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009769
Physical Location & Seq
Reverse (-)
1976743 .. 1978474
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03150.1

Sequence Viewer

Length: 918 bp
ATGCATGATTGGCTCATTCTGTTAGTTCTCGCCGGAATTGATCTCGGTTTGAACCTGATAGAGCCGTTTCACCGCTTTGTTGGGGAGGGCATGATGACAGACCTCAAGTACCCTTTGAAAGACAATACAGTTCCCTTCTGGGGTGTCCCGATAATTGCGGTACTGTTGCCGCTTGCTGTCATTCTTGTGTACTACTTCATCCGAAAGGATGTCTATGATCTGCACCAGGCCATTTTGGGCCTTCTATTCTCTGTCTTCATAACCGCCATTTTAACTGATGCCATCAAAGATGGTGTTGGTCGACCTCGTCCAGACTTCTTTTGGCGTTGTTTCCCTGATGGAAAAGGGGTATTTGATCCTACCACAAAGGATGTCATGTGTACTGGGAGCAAGAGTGTCATTAAGGAAGGACATAAAAGCTTCCCAAGCGGGCATACTTCATGGTCCTTTGCAGGTCTTGGTTTCCTTGCATGGTACTTATCGGGGAAAGTAAGGGCGTTTGATCGTAGAGGACATGTTGATAAGCTTTGTATTGTCTTTCTACCACTACTTATTGCAGCACTGGTGGGAGTTTCTCGAGTTGATGACTATTGGCATCATTGGCAAGATGTTTGTGCTGGCAGCCTTATAGGAATCACCATCGCTTCATTTTGTTACCTGCAATTCTTTCCACCTCCATACGACGCAGATGGTTGGGGACCTCATGCATACTTTCAGATGCTGGCAGAATCTCAAAATCGCGATGAATCCTCGTCTAACAATGCCAATAGTCTTAGTGTGCAGCCTGCAGAGCTTACGAGTATATACGTCCAACCTCGGAATGGCGCTGAATTATCGCGAGGCAATAGCGGAGACACGAACTACATGCTACACGGACACGAAAATGGGAGAAGGCACCCGAGTATAGGCTGTTTGTAG

Protein Analysis

306

Amino Acids

34.05

Weight (kDa)

6.29

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 78 - 223 1.4e-30 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 443, 666
AccB1I GGYRCC 1 cut(s) 894
AccI GTMKAC 1 cut(s) 301
AccII CGCG 2 cut(s) 741, 838
AciI CCGC 6 cut(s) 73, 158, 170, 264, 429, 849
AclWI GGATC 1 cut(s) 350
AfaI GTAC 5 cut(s) 110, 162, 191, 382, 476
AfiI CCNNNNNNNGG 3 cut(s) 140, 821, 905
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 2 cut(s) 52, 118
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 420, 526, 793
AluI AGCT 3 cut(s) 420, 526, 793
Alw26I GTCTC 1 cut(s) 846
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 2 cut(s) 576, 898
AoxI GGCC 2 cut(s) 228, 238
ApeKI GCWGC 3 cut(s) 557, 621, 781
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 3 cut(s) 238, 444, 698
AsuHPI GGTGA 2 cut(s) 62, 628
AvaI CYCGRG 2 cut(s) 576, 898
AvaII GGWCC 2 cut(s) 444, 698
BanI GGYRCC 1 cut(s) 894
BbsI GAAGAC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 569, 633, 793
BccI CCATC 5 cut(s) 284, 290, 332, 647, 683
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 846
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 1 cut(s) 828
BfuAI ACCTGC 2 cut(s) 443, 666
BisI GCNGC 4 cut(s) 170, 558, 622, 782
BlsI GCNGC 4 cut(s) 171, 559, 623, 783
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 2 cut(s) 444, 698
BmeT110I CYCGRG 2 cut(s) 576, 898
BmgT120I GGNCC 3 cut(s) 238, 444, 698
BmiI GGNNCC 2 cut(s) 699, 896
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 3 cut(s) 268, 604, 708
BmuI ACTGGG 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 815
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 821, 905
Bse1I ACTGG 2 cut(s) 388, 567
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 815
BseGI GGATG 3 cut(s) 198, 214, 376
BseLI CCNNNNNNNGG 3 cut(s) 140, 821, 905
BseNI ACTGG 2 cut(s) 388, 567
BseXI GCAGC 3 cut(s) 569, 633, 793
BsgI GTGCAG 2 cut(s) 206, 800
Bsh1236I CGCG 2 cut(s) 741, 838
BshFI GGCC 2 cut(s) 230, 240
BshNI GGYRCC 1 cut(s) 894
BsiHKCI CYCGRG 2 cut(s) 576, 898
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 131, 711
BslI CCNNNNNNNGG 3 cut(s) 140, 821, 905
BsmAI GTCTC 1 cut(s) 846
BsmFI GGGAC 2 cut(s) 131, 711
BsnI GGCC 2 cut(s) 230, 240
BsoBI CYCGRG 2 cut(s) 576, 898
Bsp143I GATC 4 cut(s) 40, 217, 355, 502
Bsp68I TCGCGA 2 cut(s) 741, 838
BspACI CCGC 6 cut(s) 73, 158, 170, 264, 429, 849
BspANI GGCC 2 cut(s) 230, 240
BspFNI CGCG 2 cut(s) 741, 838
BspLI GGNNCC 2 cut(s) 699, 896
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 2 cut(s) 443, 666
BspPI GGATC 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 894
BsrI ACTGG 2 cut(s) 388, 567
BssECI CCNNGG 1 cut(s) 815
BssMI GATC 4 cut(s) 40, 217, 355, 502
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 130, 165
BstC8I GCNNGC 5 cut(s) 174, 431, 619, 723, 786
BstDEI CTNAG 1 cut(s) 773
BstF5I GGATG 3 cut(s) 198, 214, 376
BstFNI CGCG 2 cut(s) 741, 838
BstH2I RGCGCY 1 cut(s) 828
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 4 cut(s) 43, 220, 358, 505
BstMAI GTCTC 1 cut(s) 846
BstMBI GATC 4 cut(s) 40, 217, 355, 502
BstMWI GCNNNNNNNGC 4 cut(s) 10, 426, 601, 790
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 2 cut(s) 518, 868
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 2 cut(s) 741, 838
BstV1I GCAGC 3 cut(s) 569, 633, 793
BstV2I GAAGAC 1 cut(s) 247
BsuRI GGCC 2 cut(s) 230, 240
BtgZI GCGATG 2 cut(s) 625, 756
BtsCI GGATG 3 cut(s) 198, 214, 376
BtsIMutI CAGTG 1 cut(s) 560
BtuMI TCGCGA 2 cut(s) 741, 838
BveI ACCTGC 2 cut(s) 443, 666
Cac8I GCNNGC 5 cut(s) 174, 431, 619, 723, 786
CaiI CAGNNNCTG 1 cut(s) 721
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 3 cut(s) 238, 444, 698
CseI GACGC 1 cut(s) 692
Csp6I GTAC 5 cut(s) 109, 161, 190, 381, 475
CviAII CATG 8 cut(s) 5, 91, 376, 441, 471, 515, 704, 865
CviQI GTAC 5 cut(s) 109, 161, 190, 381, 475
DdeI CTNAG 1 cut(s) 773
DpnI GATC 4 cut(s) 42, 219, 357, 504
DpnII GATC 4 cut(s) 40, 217, 355, 502
Eco47I GGWCC 2 cut(s) 444, 698
Eco88I CYCGRG 2 cut(s) 576, 898
EcoO109I RGGNCCY 1 cut(s) 698
EcoRII CCWGG 1 cut(s) 225
EcoT22I ATGCAT 2 cut(s) 6, 709
FaeI CATG 8 cut(s) 8, 94, 379, 444, 474, 518, 707, 868
FaqI GGGAC 2 cut(s) 131, 711
FatI CATG 8 cut(s) 4, 90, 375, 440, 470, 514, 703, 864
FauI CCCGC 1 cut(s) 422
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 4 cut(s) 170, 558, 622, 782
FokI GGATG 3 cut(s) 185, 221, 383
Fsp4HI GCNGC 4 cut(s) 170, 558, 622, 782
GlaI GCGC 1 cut(s) 826
GluI GCNGC 4 cut(s) 170, 558, 622, 782
HaeII RGCGCY 1 cut(s) 828
HaeIII GGCC 2 cut(s) 230, 240
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 692
HhaI GCGC 1 cut(s) 827
Hin1II CATG 8 cut(s) 8, 94, 379, 444, 474, 518, 707, 868
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HindIII AAGCTT 2 cut(s) 418, 524
HinfI GANTC 3 cut(s) 633, 728, 746
HpaII CCGG 1 cut(s) 33
HphI GGTGA 2 cut(s) 62, 628
Hpy166II GTNNAC 3 cut(s) 190, 302, 381
Hpy188I TCNGA 3 cut(s) 203, 717, 819
Hpy188III TCNNGA 5 cut(s) 148, 311, 576, 740, 837
Hpy8I GTNNAC 3 cut(s) 190, 302, 381
Hpy99I CGWCG 1 cut(s) 686
HpyAV CCTTC 4 cut(s) 145, 251, 401, 885
HpyCH4III ACNGT 2 cut(s) 130, 165
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 9 cut(s) 4, 223, 452, 470, 557, 661, 707, 781, 788
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 426, 601, 790
HpyF3I CTNAG 1 cut(s) 773
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 8 cut(s) 8, 94, 379, 444, 474, 518, 707, 868
HspAI GCGC 1 cut(s) 825
Kzo9I GATC 4 cut(s) 40, 217, 355, 502
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 3 cut(s) 569, 633, 793
LweI GCATC 3 cut(s) 268, 604, 708
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 1 cut(s) 653
MalI GATC 4 cut(s) 42, 219, 357, 504
MboI GATC 4 cut(s) 40, 217, 355, 502
MboII GAAGA 1 cut(s) 247
MluCI AATT 4 cut(s) 36, 153, 662, 830
MmeI TCCRAC 1 cut(s) 835
MnlI CCTC 9 cut(s) 79, 113, 315, 503, 684, 711, 760, 825, 833
Mph1103I ATGCAT 2 cut(s) 6, 709
MseI TTAA 2 cut(s) 272, 402
MslI CAYNNNNRTG 2 cut(s) 185, 882
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 2 cut(s) 741, 838
MwoI GCNNNNNNNGC 4 cut(s) 10, 426, 601, 790
NdeII GATC 4 cut(s) 40, 217, 355, 502
NlaIII CATG 8 cut(s) 8, 94, 379, 444, 474, 518, 707, 868
NlaIV GGNNCC 2 cut(s) 699, 896
NruI TCGCGA 2 cut(s) 741, 838
NsiI ATGCAT 2 cut(s) 6, 709
NspI RCATGY 2 cut(s) 518, 868
PaeR7I CTCGAG 1 cut(s) 576
PciI ACATGT 1 cut(s) 514
PfeI GAWTC 3 cut(s) 633, 728, 746
PflFI GACNNNGTC 1 cut(s) 306
PkrI GCNGC 4 cut(s) 171, 559, 623, 783
PpuMI RGGWCCY 1 cut(s) 698
PscI ACATGT 1 cut(s) 514
Psp5II RGGWCCY 1 cut(s) 698
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 699, 896
PspPI GGNCC 3 cut(s) 238, 444, 698
PspPPI RGGWCCY 1 cut(s) 698
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 721
PsyI GACNNNGTC 1 cut(s) 306
RruI TCGCGA 2 cut(s) 741, 838
RsaI GTAC 5 cut(s) 110, 162, 191, 382, 476
RsaNI GTAC 5 cut(s) 109, 161, 190, 381, 475
RseI CAYNNNNRTG 2 cut(s) 185, 882
SalI GTCGAC 1 cut(s) 300
SaqAI TTAA 2 cut(s) 272, 402
SatI GCNGC 4 cut(s) 170, 558, 622, 782
Sau3AI GATC 4 cut(s) 40, 217, 355, 502
Sau96I GGNCC 3 cut(s) 238, 444, 698
ScrFI CCNGG 1 cut(s) 227
SfaNI GCATC 3 cut(s) 268, 604, 708
SfcI CTRYAG 1 cut(s) 786
Sfr274I CTCGAG 1 cut(s) 576
SinI GGWCC 2 cut(s) 444, 698
SlaI CTCGAG 1 cut(s) 576
SmiMI CAYNNNNRTG 2 cut(s) 185, 882
SmlI CTYRAG 2 cut(s) 104, 576
SmoI CTYRAG 2 cut(s) 104, 576
Sse9I AATT 4 cut(s) 36, 153, 662, 830
SsiI CCGC 6 cut(s) 73, 158, 170, 264, 429, 849
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 2 cut(s) 130, 165
TaiI ACGT 1 cut(s) 810
TaqI TCGA 2 cut(s) 301, 577
TasI AATT 4 cut(s) 36, 153, 662, 830
TatI WGTACW 2 cut(s) 189, 380
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 3 cut(s) 633, 728, 746
Tru1I TTAA 2 cut(s) 272, 402
Tru9I TTAA 2 cut(s) 272, 402
TscAI CASTG 1 cut(s) 567
TseI GCWGC 3 cut(s) 557, 621, 781
TspDTI ATGAA 5 cut(s) 187, 247, 429, 636, 759
TspGWI ACGGA 1 cut(s) 888
TspRI CASTG 1 cut(s) 567
Tth111I GACNNNGTC 1 cut(s) 306
VpaK11BI GGWCC 2 cut(s) 444, 698
XceI RCATGY 2 cut(s) 518, 868
XcmI CCANNNNNNNNNTGG 2 cut(s) 318, 818
XhoI CTCGAG 1 cut(s) 576
XmiI GTMKAC 1 cut(s) 301
Zsp2I ATGCAT 2 cut(s) 6, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.