FvH4_4g27400

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014725
Physical Location & Seq
Forward (+)
28413275 .. 28417004
3730 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27400.t1

Sequence Viewer

Length: 792 bp
ATGGCAGGGAGAAATCGTATGCCTCGCCATGCTGATGGCTTTCGAGGTTATCGTGATGATCCTCGGCCTGGTATTCACCGTCGACCAGAGGCATTTTCTATTCACCCTGCAGCTTTGGAAGAAGAACTTGCAATGCAGCACAGAGAAATGCAACGAATAATTGCTGAGAATCGGCTTGTAATTGATGACAATACTGTTCTTCAAAGGGCGTTAACAGATGCAAAAGATGAGATTCATAGATTAGGGCAGGTCATACCCAAACTGCGGGTTGAGAAAGAGGCAGAGACTAGGGAGTTGATTGAGAGAGGATTGAAGCTTGAAGCTGATCTCCGTGCTGTTGAGCCTATAAGGGCAGAGGTCGTGCAATTGAGAGCTGAAATTCAGAAATTAAATGCTCTACGGCAGGAGTTGTCTACCCAAATCCAAGGTCTCACTCAGGAAGTCACTCGATTACAAGCTGAGAATCAGCAACTAGTTGCCATGAGGACTGATGTCGATGGCATGCGAAAAGAGCTTGTCGAGACAAGGAGAGCTTATGAATATGAGAGGAAAGCCAATGAAGAACAAGTTGGACAAAAGCAAGCAATGGAAAAGAACCTGATTGATATGGCTCGAGAAATAGAGCAGCTACGAGCAGAGCAGATGAATGCAGACAGGAGAGCACGGGGACTAGGTGGCGGGGGTTATGGAATGATGAATGGAGGTCCTGAAATGAGATATTCAGGTGCTTTGTATGGCAGTGCCTCTAGAAATGGTTGGGGACCTTATGACAGGCCTGGGTCACGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

30.16

Weight (kDa)

8.82

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccI GTMKAC 2 cut(s) 82, 413
AciI CCGC 2 cut(s) 265, 678
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 1 cut(s) 378
AfiI CCNNNNNNNGG 2 cut(s) 68, 264
AgsI TTSAA 3 cut(s) 203, 313, 320
AhlI ACTAGT 1 cut(s) 472
AjnI CCWGG 2 cut(s) 67, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 8 cut(s) 113, 316, 323, 374, 458, 514, 533, 628
AluI AGCT 8 cut(s) 113, 316, 323, 374, 458, 514, 533, 628
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 3 cut(s) 278, 434, 515
AlwI GGATC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 612
AoxI GGCC 2 cut(s) 65, 773
ApeKI GCWGC 3 cut(s) 110, 136, 625
ApoI RAATTY 1 cut(s) 378
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
AspS9I GGNCC 2 cut(s) 704, 761
AsuHPI GGTGA 2 cut(s) 68, 95
AvaI CYCGRG 1 cut(s) 612
AvaII GGWCC 2 cut(s) 704, 761
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 122, 148, 637
BccI CCATC 2 cut(s) 29, 491
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 2 cut(s) 69, 777
BcoDI GTCTC 3 cut(s) 278, 434, 515
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 4 cut(s) 288, 473, 671, 747
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 3 cut(s) 111, 137, 626
BlsI GCNGC 3 cut(s) 112, 138, 627
Bme1390I CCNGG 2 cut(s) 69, 777
Bme18I GGWCC 2 cut(s) 704, 761
BmeT110I CYCGRG 1 cut(s) 612
BmgT120I GGNCC 2 cut(s) 704, 761
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 2 cut(s) 69, 777
BmsI GCATC 1 cut(s) 208
BoxI GACNNNNGTC 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 62, 424, 776
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 264
Bse3DI GCAATG 2 cut(s) 138, 591
BseBI CCWGG 2 cut(s) 69, 777
BseDI CCNNGG 3 cut(s) 62, 424, 776
BseLI CCNNNNNNNGG 2 cut(s) 68, 264
BseMI GCAATG 2 cut(s) 138, 591
BseMII CTCAG 3 cut(s) 156, 449, 450
BseXI GCAGC 3 cut(s) 122, 148, 637
BshFI GGCC 2 cut(s) 67, 775
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 612
BslFI GGGAC 2 cut(s) 681, 774
BslI CCNNNNNNNGG 2 cut(s) 68, 264
BsmAI GTCTC 3 cut(s) 278, 434, 515
BsmFI GGGAC 2 cut(s) 681, 774
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 2 cut(s) 67, 775
Bso31I GGTCTC 1 cut(s) 434
BsoBI CYCGRG 1 cut(s) 612
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 2 cut(s) 58, 325
BspACI CCGC 2 cut(s) 265, 678
BspANI GGCC 2 cut(s) 67, 775
BspCNI CTCAG 3 cut(s) 157, 448, 451
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 138, 591
BssECI CCNNGG 3 cut(s) 62, 424, 776
BssMI GATC 2 cut(s) 58, 325
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 2 cut(s) 69, 777
Bst4CI ACNGT 2 cut(s) 80, 196
BstC8I GCNNGC 2 cut(s) 503, 582
BstDEI CTNAG 3 cut(s) 165, 435, 459
BstKTI GATC 2 cut(s) 61, 328
BstMAI GTCTC 3 cut(s) 278, 434, 515
BstMBI GATC 2 cut(s) 58, 325
BstMWI GCNNNNNNNGC 1 cut(s) 511
BstNI CCWGG 2 cut(s) 69, 777
BstNSI RCATGY 1 cut(s) 505
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 2 cut(s) 67, 775
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 3 cut(s) 122, 148, 637
BstXI CCANNNNNNTGG 1 cut(s) 35
BsuRI GGCC 2 cut(s) 67, 775
BtsI GCAGTG 1 cut(s) 745
BtsIMutI CAGTG 1 cut(s) 745
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 2 cut(s) 503, 582
Cfr13I GGNCC 2 cut(s) 704, 761
CviAII CATG 3 cut(s) 29, 481, 502
DdeI CTNAG 3 cut(s) 165, 435, 459
DpnI GATC 2 cut(s) 60, 327
DpnII GATC 2 cut(s) 58, 325
Eco130I CCWWGG 1 cut(s) 424
Eco147I AGGCCT 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 704, 761
Eco88I CYCGRG 1 cut(s) 612
EcoO109I RGGNCCY 2 cut(s) 704, 761
EcoRII CCWGG 2 cut(s) 67, 775
EcoT14I CCWWGG 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 3 cut(s) 32, 484, 505
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 517, 549
FaqI GGGAC 2 cut(s) 681, 774
FatI CATG 3 cut(s) 28, 480, 501
FauI CCCGC 2 cut(s) 258, 671
FblI GTMKAC 2 cut(s) 82, 413
Fnu4HI GCNGC 3 cut(s) 111, 137, 626
Fsp4HI GCNGC 3 cut(s) 111, 137, 626
FspBI CTAG 4 cut(s) 288, 473, 671, 747
GluI GCNGC 3 cut(s) 111, 137, 626
HaeIII GGCC 2 cut(s) 67, 775
Hin1II CATG 3 cut(s) 32, 484, 505
HincII GTYRAC 2 cut(s) 83, 213
HindII GTYRAC 2 cut(s) 83, 213
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 3 cut(s) 169, 232, 463
HpaI GTTAAC 1 cut(s) 213
HphI GGTGA 2 cut(s) 68, 95
Hpy166II GTNNAC 3 cut(s) 83, 213, 414
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 6 cut(s) 53, 437, 520, 614, 707, 747
Hpy8I GTNNAC 3 cut(s) 83, 213, 414
Hpy99I CGWCG 1 cut(s) 84
HpyCH4III ACNGT 2 cut(s) 80, 196
HpyCH4V TGCA 7 cut(s) 110, 131, 136, 151, 221, 364, 650
HpyF10VI GCNNNNNNNGC 1 cut(s) 511
HpyF3I CTNAG 3 cut(s) 165, 435, 459
Hsp92II CATG 3 cut(s) 32, 484, 505
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 2 cut(s) 58, 325
Lsp1109I GCAGC 3 cut(s) 122, 148, 637
LweI GCATC 1 cut(s) 208
MaeI CTAG 4 cut(s) 288, 473, 671, 747
MaeIII GTNAC 2 cut(s) 442, 780
MalI GATC 2 cut(s) 60, 327
MboI GATC 2 cut(s) 58, 325
MboII GAAGA 4 cut(s) 131, 134, 191, 572
MfeI CAATTG 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 5 cut(s) 159, 180, 365, 378, 386
MmeI TCCRAC 1 cut(s) 550
MseI TTAA 3 cut(s) 212, 389, 790
MslI CAYNNNNRTG 1 cut(s) 33
MspR9I CCNGG 2 cut(s) 69, 777
MunI CAATTG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 2 cut(s) 69, 777
MwoI GCNNNNNNNGC 1 cut(s) 511
NdeII GATC 2 cut(s) 58, 325
NlaIII CATG 3 cut(s) 32, 484, 505
NlaIV GGNNCC 1 cut(s) 762
NmeAIII GCCGAG 1 cut(s) 43
NmuCI GTSAC 2 cut(s) 442, 780
NspI RCATGY 1 cut(s) 505
PaeI GCATGC 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 612
PceI AGGCCT 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 3 cut(s) 169, 232, 463
PkrI GCNGC 3 cut(s) 112, 138, 627
PpuMI RGGWCCY 2 cut(s) 704, 761
PshAI GACNNNNGTC 1 cut(s) 491
Psp5II RGGWCCY 2 cut(s) 704, 761
Psp6I CCWGG 2 cut(s) 67, 775
PspGI CCWGG 2 cut(s) 67, 775
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 2 cut(s) 704, 761
PspPPI RGGWCCY 2 cut(s) 704, 761
PstI CTGCAG 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 33
SalI GTCGAC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 212, 389, 790
SatI GCNGC 3 cut(s) 111, 137, 626
Sau3AI GATC 2 cut(s) 58, 325
Sau96I GGNCC 2 cut(s) 704, 761
ScrFI CCNGG 2 cut(s) 69, 777
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 108
Sfr274I CTCGAG 1 cut(s) 612
SinI GGWCC 2 cut(s) 704, 761
SlaI CTCGAG 1 cut(s) 612
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SpeI ACTAGT 1 cut(s) 472
SphI GCATGC 1 cut(s) 505
Sse9I AATT 5 cut(s) 159, 180, 365, 378, 386
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 2 cut(s) 265, 678
SspMI CTAG 4 cut(s) 288, 473, 671, 747
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 2 cut(s) 67, 775
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 80, 196
TaqI TCGA 6 cut(s) 43, 82, 448, 495, 519, 613
TasI AATT 5 cut(s) 159, 180, 365, 378, 386
TfiI GAWTC 3 cut(s) 169, 232, 463
Tru1I TTAA 3 cut(s) 212, 389, 790
Tru9I TTAA 3 cut(s) 212, 389, 790
TscAI CASTG 1 cut(s) 745
TseFI GTSAC 2 cut(s) 442, 780
TseI GCWGC 3 cut(s) 110, 136, 625
Tsp45I GTSAC 2 cut(s) 442, 780
TspDTI ATGAA 5 cut(s) 224, 552, 573, 659, 710
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 1 cut(s) 745
VpaK11BI GGWCC 2 cut(s) 704, 761
XapI RAATTY 1 cut(s) 378
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 1 cut(s) 505
XhoI CTCGAG 1 cut(s) 612
XmiI GTMKAC 2 cut(s) 82, 413
XspI CTAG 4 cut(s) 288, 473, 671, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.