Rh4AG338800

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014725
Physical Location & Seq
Forward (+)
64760140 .. 64766075
5936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG338800.1

Sequence Viewer

Length: 1371 bp
ATGTTGTTCAGCTTTCCCAGTGTCGTTAACATTTGGAGCAAATTGCTGGAAAGTAATCCAACTATGTTTCAAAATAGTAAAACTGCTGAATCAGCTTTTGGTGCAGCAATCAAGGTTGTGAGAGATATCCAAGGAAAGTCTTTTATTCTCTATGGCACTACTTCAAAAGTCTCGTTTGTAGCTGCTGATGTTGATGGCAATGTTCCTTTCTTCTGGGGAATTGATTTTGAAGGCAATCTTGTTATTGCTGATGATGCAGAAGTTGTGAAGGACCGTGGGAAGTTGTTTGCACCATTTCCTAAAGGGAGCTTATCAACAGAACGGAAACTTAGAGAAAATGATAGAGTGGTGGCTGAAGGAAAGATATCATCAACAAACCGAAGTCCAAAAGTTCATTGTGTGCCTCTTGGACAAGAGTGTTGGAAGGTTTGGGTTGATGTGGCGCTTGATGATCTAGCCTGCTTCTTTTGGTTTACTATAAAACTGGAAGTATGTACCAGAACTGGAACCAAAGCATCGTTCCCGTTGAAGATCGAAGCTTTTTCAGGCAGTCACTCCTTCTCTTCTCTGATCACTATTATGGCAGGGAGAAATCGTATGCCTCGCCATGCTGATGGCTTTCGAGGTTATCGTGATGATCCTCGGCCTGTTATGCACCGTGGACCACAACCGTTTTCTATTCACCCTGCAGCTTTGGAAGAAGAACTTGCAATGCAGCACAGAGAAATGCAACGAATAATCGCTGAGAATCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGGAATTAACAGATGCAAAAGATGAGATTCATAGATTAGGGCAGGTCATACCCAAACTCCGGGCTGAGAAAGAGGCAGAGACTAGGGAGTTGATTGAGAGAGGATTAAAACTTGAAGCTGATCTCCGTGCTGTTGAGCCTGTAGGGGCAGAGGTTTTGCAATTGAGAGCTGAAATTCAGAAATTAAATGCTTTACGGCAGGAGTTGTCTACCCAAGTCCAAGGTCTCACTCAAGACGTCACTCGATTACAAGCTGAGAATCAGCAACTAGTTGCCATGAGGGCTGATATTGATGGCATGCAAAAAGAGCTTGTTGAGACAAGGAGAGCTTATGAATATGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTGATTTCCATGGCTCGTGAAATAGAGCAGCTACGAGCAGAGCAGATGAATGCAGGCAGGAGGGCACGGGGGCTAGGTGGCGGGGGTTATGGAATGATGAGTGGAGGTCCTGAAATGAGATATTCCGGTGGTTCGTATGGCAATGTCTATAGAAATGGTTGGGGACCTTATGACAGGCCTGGACCACGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

456

Amino Acids

51.41

Weight (kDa)

7.68

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 36 - 106 1.8e-19 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1023
Acc36I ACCTGC 1 cut(s) 817
AccI GTMKAC 1 cut(s) 992
AciI CCGC 1 cut(s) 1257
AclWI GGATC 1 cut(s) 632
AcsI RAATTY 1 cut(s) 957
AcuI CTGAAG 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 1020
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 1 cut(s) 843
AgsI TTSAA 7 cut(s) 71, 165, 230, 529, 782, 899, 1151
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 1 cut(s) 1354
AjuI GAANNNNNNNTTGG 4 cut(s) 81, 113, 503, 535
AloI GAACNNNNNNTCC 2 cut(s) 1166, 1198
Alw26I GTCTC 4 cut(s) 175, 857, 1013, 1094
AlwI GGATC 1 cut(s) 632
AoxI GGCC 2 cut(s) 644, 1352
ApeKI GCWGC 5 cut(s) 104, 182, 689, 715, 1204
ApoI RAATTY 1 cut(s) 957
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 5 cut(s) 271, 662, 1283, 1340, 1358
AsuC2I CCSGG 1 cut(s) 845
AsuHPI GGTGA 1 cut(s) 674
AvaII GGWCC 5 cut(s) 271, 662, 1283, 1340, 1358
BaeGI GKGCMC 1 cut(s) 1243
BauI CACGAG 1 cut(s) 1191
BbvI GCAGC 5 cut(s) 116, 169, 701, 727, 1216
BccI CCATC 3 cut(s) 188, 608, 1070
BceAI ACGGC 1 cut(s) 995
BciT130I CCWGG 1 cut(s) 1356
BclI TGATCA 1 cut(s) 570
BcnI CCSGG 1 cut(s) 845
BcoDI GTCTC 4 cut(s) 175, 857, 1013, 1094
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 4 cut(s) 455, 867, 1052, 1250
BfmI CTRYAG 3 cut(s) 687, 924, 1324
BfoI RGCGCY 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 817
BglI GCCNNNNNGGC 1 cut(s) 1064
BisI GCNGC 5 cut(s) 105, 183, 690, 716, 1205
BlsI GCNGC 5 cut(s) 106, 184, 691, 717, 1206
Bme1390I CCNGG 2 cut(s) 845, 1356
Bme18I GGWCC 5 cut(s) 271, 662, 1283, 1340, 1358
BmgT120I GGNCC 5 cut(s) 271, 662, 1283, 1340, 1358
BmiI GGNNCC 2 cut(s) 508, 1341
BmrFI CCNGG 2 cut(s) 845, 1356
BmrI ACTGGG 1 cut(s) 12
BmsI GCATC 3 cut(s) 244, 524, 787
BmuI ACTGGG 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 999
BpuMI CCSGG 1 cut(s) 845
BsaHI GRCGYC 1 cut(s) 1020
BsaI GGTCTC 1 cut(s) 1013
BsaJI CCNNGG 7 cut(s) 130, 274, 641, 658, 1003, 1185, 1361
BsaWI WCCGGW 1 cut(s) 1301
BsaXI ACNNNNNCTCC 4 cut(s) 580, 610, 825, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 843
Bse1I ACTGG 3 cut(s) 18, 489, 508
Bse3DI GCAATG 4 cut(s) 205, 717, 1170, 1324
BseBI CCWGG 1 cut(s) 1356
BseDI CCNNGG 7 cut(s) 130, 274, 641, 658, 1003, 1185, 1361
BseLI CCNNNNNNNGG 1 cut(s) 843
BseMI GCAATG 4 cut(s) 205, 717, 1170, 1324
BseMII CTCAG 3 cut(s) 735, 840, 1029
BseNI ACTGG 3 cut(s) 18, 489, 508
BseSI GKGCMC 1 cut(s) 1243
BseXI GCAGC 5 cut(s) 116, 169, 701, 727, 1216
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 2 cut(s) 646, 1354
BsiSI CCGG 2 cut(s) 844, 1302
BslFI GGGAC 1 cut(s) 1353
BslI CCNNNNNNNGG 1 cut(s) 843
BsmAI GTCTC 4 cut(s) 175, 857, 1013, 1094
BsmFI GGGAC 1 cut(s) 1353
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 2 cut(s) 646, 1354
Bso31I GGTCTC 1 cut(s) 1013
Bsp1286I GDGCHC 1 cut(s) 1243
Bsp143I GATC 5 cut(s) 451, 531, 570, 637, 904
Bsp19I CCATGG 1 cut(s) 1185
BspACI CCGC 1 cut(s) 1257
BspANI GGCC 2 cut(s) 646, 1354
BspCNI CTCAG 3 cut(s) 736, 841, 1030
BspLI GGNNCC 2 cut(s) 508, 1341
BspMAI CTGCAG 1 cut(s) 691
BspMI ACCTGC 1 cut(s) 817
BspPI GGATC 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 1013
BsrDI GCAATG 4 cut(s) 205, 717, 1170, 1324
BsrI ACTGG 3 cut(s) 18, 489, 508
BssECI CCNNGG 7 cut(s) 130, 274, 641, 658, 1003, 1185, 1361
BssMI GATC 5 cut(s) 451, 531, 570, 637, 904
BssNI GRCGYC 1 cut(s) 1020
BssSI CACGAG 1 cut(s) 1191
BssT1I CCWWGG 3 cut(s) 130, 1003, 1185
Bst2BI CACGAG 1 cut(s) 1191
Bst2UI CCWGG 1 cut(s) 1356
Bst4CI ACNGT 3 cut(s) 275, 659, 672
Bst6I CTCTTC 1 cut(s) 568
BstACI GRCGYC 1 cut(s) 1020
BstC8I GCNNGC 4 cut(s) 460, 1082, 1161, 1231
BstDEI CTNAG 4 cut(s) 329, 744, 849, 1038
BstDSI CCRYGG 4 cut(s) 274, 658, 1185, 1361
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 5 cut(s) 454, 534, 573, 640, 907
BstMAI GTCTC 4 cut(s) 175, 857, 1013, 1094
BstMBI GATC 5 cut(s) 451, 531, 570, 637, 904
BstMWI GCNNNNNNNGC 6 cut(s) 92, 101, 254, 652, 1064, 1090
BstNI CCWGG 1 cut(s) 1356
BstNSI RCATGY 1 cut(s) 1084
BstSCI CCNGG 2 cut(s) 843, 1354
BstSFI CTRYAG 3 cut(s) 687, 924, 1324
BstSLI GKGCMC 1 cut(s) 1243
BstV1I GCAGC 5 cut(s) 116, 169, 701, 727, 1216
BstXI CCANNNNNNTGG 1 cut(s) 614
BsuRI GGCC 2 cut(s) 646, 1354
BtgI CCRYGG 4 cut(s) 274, 658, 1185, 1361
BtsIMutI CAGTG 1 cut(s) 25
BveI ACCTGC 1 cut(s) 817
Cac8I GCNNGC 4 cut(s) 460, 1082, 1161, 1231
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 5 cut(s) 271, 662, 1283, 1340, 1358
Csp6I GTAC 1 cut(s) 495
CviAII CATG 4 cut(s) 608, 1060, 1081, 1186
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 4 cut(s) 329, 744, 849, 1038
DpnI GATC 5 cut(s) 453, 533, 572, 639, 906
DpnII GATC 5 cut(s) 451, 531, 570, 637, 904
Eam1104I CTCTTC 1 cut(s) 568
EarI CTCTTC 1 cut(s) 568
Eco130I CCWWGG 3 cut(s) 130, 1003, 1185
Eco147I AGGCCT 1 cut(s) 1354
Eco31I GGTCTC 1 cut(s) 1013
Eco32I GATATC 2 cut(s) 127, 366
Eco47I GGWCC 5 cut(s) 271, 662, 1283, 1340, 1358
Eco57I CTGAAG 1 cut(s) 375
EcoO109I RGGNCCY 2 cut(s) 1283, 1340
EcoRII CCWGG 1 cut(s) 1354
EcoRV GATATC 2 cut(s) 127, 366
EcoT14I CCWWGG 3 cut(s) 130, 1003, 1185
ErhI CCWWGG 3 cut(s) 130, 1003, 1185
FaeI CATG 4 cut(s) 611, 1063, 1084, 1189
FalI AAGNNNNNCTT 6 cut(s) 222, 254, 690, 722, 1096, 1128
FaqI GGGAC 1 cut(s) 1353
FatI CATG 4 cut(s) 607, 1059, 1080, 1185
FauI CCCGC 1 cut(s) 1250
FbaI TGATCA 1 cut(s) 570
FblI GTMKAC 1 cut(s) 992
Fnu4HI GCNGC 5 cut(s) 105, 183, 690, 716, 1205
Fsp4HI GCNGC 5 cut(s) 105, 183, 690, 716, 1205
FspBI CTAG 4 cut(s) 455, 867, 1052, 1250
GlaI GCGC 1 cut(s) 444
GluI GCNGC 5 cut(s) 105, 183, 690, 716, 1205
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 2 cut(s) 646, 1354
HapII CCGG 2 cut(s) 844, 1302
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 1020
Hin1II CATG 4 cut(s) 611, 1063, 1084, 1189
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 4 cut(s) 89, 748, 811, 1042
HpaI GTTAAC 1 cut(s) 28
HpaII CCGG 2 cut(s) 844, 1302
HphI GGTGA 1 cut(s) 674
Hpy166II GTNNAC 4 cut(s) 28, 474, 662, 993
Hpy188I TCNGA 2 cut(s) 570, 963
Hpy188III TCNNGA 4 cut(s) 632, 1016, 1193, 1286
Hpy8I GTNNAC 4 cut(s) 28, 474, 662, 993
HpyAV CCTTC 6 cut(s) 224, 262, 350, 418, 568, 785
HpyCH4III ACNGT 3 cut(s) 275, 659, 672
HpyCH4IV ACGT 1 cut(s) 1020
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 101, 254, 652, 1064, 1090
HpyF3I CTNAG 4 cut(s) 329, 744, 849, 1038
HpySE526I ACGT 1 cut(s) 1020
Hsp92I GRCGYC 1 cut(s) 1020
Hsp92II CATG 4 cut(s) 611, 1063, 1084, 1189
HspAI GCGC 1 cut(s) 443
Ksp22I TGATCA 1 cut(s) 570
KspAI GTTAAC 1 cut(s) 28
Kzo9I GATC 5 cut(s) 451, 531, 570, 637, 904
LmnI GCTCC 2 cut(s) 36, 306
Lsp1109I GCAGC 5 cut(s) 116, 169, 701, 727, 1216
LweI GCATC 3 cut(s) 244, 524, 787
MaeI CTAG 4 cut(s) 455, 867, 1052, 1250
MaeII ACGT 1 cut(s) 1020
MaeIII GTNAC 2 cut(s) 551, 1021
MalI GATC 5 cut(s) 453, 533, 572, 639, 906
MboI GATC 5 cut(s) 451, 531, 570, 637, 904
MboII GAAGA 7 cut(s) 202, 541, 555, 710, 713, 770, 1151
MfeI CAATTG 1 cut(s) 944
MhlI GDGCHC 1 cut(s) 1243
MluCI AATT 7 cut(s) 41, 219, 759, 788, 944, 957, 965
MmeI TCCRAC 2 cut(s) 83, 401
MseI TTAA 5 cut(s) 27, 791, 890, 968, 1369
MslI CAYNNNNRTG 2 cut(s) 578, 612
MspI CCGG 2 cut(s) 844, 1302
MspR9I CCNGG 2 cut(s) 845, 1356
MunI CAATTG 1 cut(s) 944
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 1 cut(s) 1356
MwoI GCNNNNNNNGC 6 cut(s) 92, 101, 254, 652, 1064, 1090
NciI CCSGG 1 cut(s) 845
NcoI CCATGG 1 cut(s) 1185
NdeII GATC 5 cut(s) 451, 531, 570, 637, 904
NlaIII CATG 4 cut(s) 611, 1063, 1084, 1189
NlaIV GGNNCC 2 cut(s) 508, 1341
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 2 cut(s) 551, 1021
NspI RCATGY 1 cut(s) 1084
PaeI GCATGC 1 cut(s) 1084
PceI AGGCCT 1 cut(s) 1354
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PctI GAATGC 1 cut(s) 1231
PfeI GAWTC 4 cut(s) 89, 748, 811, 1042
PkrI GCNGC 5 cut(s) 106, 184, 691, 717, 1206
PpuMI RGGWCCY 2 cut(s) 1283, 1340
Psp5II RGGWCCY 2 cut(s) 1283, 1340
Psp6I CCWGG 1 cut(s) 1354
PspGI CCWGG 1 cut(s) 1354
PspN4I GGNNCC 2 cut(s) 508, 1341
PspPI GGNCC 5 cut(s) 271, 662, 1283, 1340, 1358
PspPPI RGGWCCY 2 cut(s) 1283, 1340
PstI CTGCAG 1 cut(s) 691
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
RseI CAYNNNNRTG 2 cut(s) 578, 612
SaqAI TTAA 5 cut(s) 27, 791, 890, 968, 1369
SatI GCNGC 5 cut(s) 105, 183, 690, 716, 1205
Sau3AI GATC 5 cut(s) 451, 531, 570, 637, 904
Sau96I GGNCC 5 cut(s) 271, 662, 1283, 1340, 1358
ScrFI CCNGG 2 cut(s) 845, 1356
SduI GDGCHC 1 cut(s) 1243
SfaNI GCATC 3 cut(s) 244, 524, 787
SfcI CTRYAG 3 cut(s) 687, 924, 1324
SinI GGWCC 5 cut(s) 271, 662, 1283, 1340, 1358
SmiMI CAYNNNNRTG 2 cut(s) 578, 612
SmlI CTYRAG 1 cut(s) 1014
SmoI CTYRAG 1 cut(s) 1014
SpeI ACTAGT 1 cut(s) 1051
SphI GCATGC 1 cut(s) 1084
Sse9I AATT 7 cut(s) 41, 219, 759, 788, 944, 957, 965
SseBI AGGCCT 1 cut(s) 1354
SsiI CCGC 1 cut(s) 1257
SspMI CTAG 4 cut(s) 455, 867, 1052, 1250
StuI AGGCCT 1 cut(s) 1354
StyD4I CCNGG 2 cut(s) 843, 1354
StyI CCWWGG 3 cut(s) 130, 1003, 1185
TaaI ACNGT 3 cut(s) 275, 659, 672
TaiI ACGT 1 cut(s) 1023
TaqI TCGA 3 cut(s) 534, 622, 1027
TasI AATT 7 cut(s) 41, 219, 759, 788, 944, 957, 965
TfiI GAWTC 4 cut(s) 89, 748, 811, 1042
Tru1I TTAA 5 cut(s) 27, 791, 890, 968, 1369
Tru9I TTAA 5 cut(s) 27, 791, 890, 968, 1369
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 2 cut(s) 551, 1021
TseI GCWGC 5 cut(s) 104, 182, 689, 715, 1204
Tsp45I GTSAC 2 cut(s) 551, 1021
TspDTI ATGAA 5 cut(s) 383, 803, 1131, 1152, 1238
TspGWI ACGGA 2 cut(s) 337, 899
TspRI CASTG 1 cut(s) 25
VpaK11BI GGWCC 5 cut(s) 271, 662, 1283, 1340, 1358
XapI RAATTY 1 cut(s) 957
XceI RCATGY 1 cut(s) 1084
XmiI GTMKAC 1 cut(s) 992
XspI CTAG 4 cut(s) 455, 867, 1052, 1250
ZraI GACGTC 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.