Rh4BG346900

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014725
Physical Location & Seq
Forward (+)
50929833 .. 50930914
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG346900.1

Sequence Viewer

Length: 792 bp
ATGGCAGGGAGAAATCGTATGCCTCGCCATGCTGATGGCTTTCAAGGTTATCGTGATGATCCTCGGCCTGTTATGCACCGTGGACCACAACCGTTTTCTATTCACCCTGCAGCTTTGGAAGAAGAACTTGCAATGCAGCACAGAGAAATGCAACGAGTAATCGCTGAGAATCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGGAATTAACAGATGCAAAAGATGAGATTCATAGATTAGGGCAGGTCATACCCAAACTCCGGGCCGAGAAAGAGGCAGAGACTAGGGAGTTGATTGAGAGAGGATTGAAACTTGAAGCTGATCTCCGTGCTGTTGAGCCTGTAAGGGCAGAGGTTTTGCAATTGAGAGCTGAAATTCAGAAATTAAATGCTTTACGTCAGGAGTTGTCTACCCAAGTCCAAGGTCTCACTCAAGACGTCACTCGATTACAAGCTGAGAATCAGCAACTAGTTGCCATGAGGACTGATATTGATGGCATGCGAAAAGAGCTTGTTGAGACAAGGAGAGCTTATGAATATGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTGATTTCCATGGCTCGTGAAATAGAGCAGCTACGAGCAGAGCAGATGAATGCAGGCAGGAGGGCACGGGGGCTAGGTGGCGGGGGTTATGGAATGATGAGTGGAGGTCCTGAAATGAGATATTCCGGTGGTTCGTATGGCAATGTCTATAGAAATGGTTGGGGACCTTATGACAGGCCTGGACCACGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

30.2

Weight (kDa)

7.97

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 413
AciI CCGC 1 cut(s) 678
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 441
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 5 cut(s) 44, 203, 313, 320, 572
AhlI ACTAGT 1 cut(s) 472
AjnI CCWGG 1 cut(s) 775
AloI GAACNNNNNNTCC 2 cut(s) 587, 619
AluBI AGCT 7 cut(s) 113, 323, 374, 458, 514, 533, 628
AluI AGCT 7 cut(s) 113, 323, 374, 458, 514, 533, 628
Alw26I GTCTC 3 cut(s) 278, 434, 515
AlwI GGATC 1 cut(s) 53
AoxI GGCC 3 cut(s) 65, 267, 773
ApeKI GCWGC 3 cut(s) 110, 136, 625
ApoI RAATTY 1 cut(s) 378
AspS9I GGNCC 5 cut(s) 83, 267, 704, 761, 779
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 95
AvaII GGWCC 4 cut(s) 83, 704, 761, 779
BaeGI GKGCMC 1 cut(s) 664
BauI CACGAG 1 cut(s) 612
BbvI GCAGC 3 cut(s) 122, 148, 637
BccI CCATC 2 cut(s) 29, 491
BciT130I CCWGG 1 cut(s) 777
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 3 cut(s) 278, 434, 515
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 3 cut(s) 288, 473, 671
BfmI CTRYAG 2 cut(s) 108, 745
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 3 cut(s) 111, 137, 626
BlsI GCNGC 3 cut(s) 112, 138, 627
Bme1390I CCNGG 2 cut(s) 266, 777
Bme18I GGWCC 4 cut(s) 83, 704, 761, 779
BmgT120I GGNCC 5 cut(s) 83, 267, 704, 761, 779
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 2 cut(s) 266, 777
BmsI GCATC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 5 cut(s) 62, 79, 424, 606, 782
BsaWI WCCGGW 1 cut(s) 722
BsaXI ACNNNNNCTCC 3 cut(s) 31, 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse3DI GCAATG 3 cut(s) 138, 591, 745
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 5 cut(s) 62, 79, 424, 606, 782
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 3 cut(s) 138, 591, 745
BseMII CTCAG 2 cut(s) 156, 450
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 3 cut(s) 122, 148, 637
BshFI GGCC 3 cut(s) 67, 269, 775
BsiSI CCGG 2 cut(s) 265, 723
BslFI GGGAC 1 cut(s) 774
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 3 cut(s) 278, 434, 515
BsmFI GGGAC 1 cut(s) 774
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 3 cut(s) 67, 269, 775
Bso31I GGTCTC 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 2 cut(s) 58, 325
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 1 cut(s) 678
BspANI GGCC 3 cut(s) 67, 269, 775
BspCNI CTCAG 2 cut(s) 157, 451
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 3 cut(s) 138, 591, 745
BssECI CCNNGG 5 cut(s) 62, 79, 424, 606, 782
BssMI GATC 2 cut(s) 58, 325
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 2 cut(s) 424, 606
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 80, 93
BstACI GRCGYC 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 503, 582, 652
BstDEI CTNAG 2 cut(s) 165, 459
BstDSI CCRYGG 3 cut(s) 79, 606, 782
BstKTI GATC 2 cut(s) 61, 328
BstMAI GTCTC 3 cut(s) 278, 434, 515
BstMBI GATC 2 cut(s) 58, 325
BstMWI GCNNNNNNNGC 2 cut(s) 73, 511
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 2 cut(s) 264, 775
BstSFI CTRYAG 2 cut(s) 108, 745
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 3 cut(s) 122, 148, 637
BstXI CCANNNNNNTGG 1 cut(s) 35
BsuRI GGCC 3 cut(s) 67, 269, 775
BtgI CCRYGG 3 cut(s) 79, 606, 782
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 3 cut(s) 503, 582, 652
Cfr13I GGNCC 5 cut(s) 83, 267, 704, 761, 779
CviAII CATG 4 cut(s) 29, 481, 502, 607
DdeI CTNAG 2 cut(s) 165, 459
DpnI GATC 2 cut(s) 60, 327
DpnII GATC 2 cut(s) 58, 325
Eco130I CCWWGG 2 cut(s) 424, 606
Eco147I AGGCCT 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 4 cut(s) 83, 704, 761, 779
EcoO109I RGGNCCY 2 cut(s) 704, 761
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 2 cut(s) 424, 606
ErhI CCWWGG 2 cut(s) 424, 606
FaeI CATG 4 cut(s) 32, 484, 505, 610
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 517, 549
FaqI GGGAC 1 cut(s) 774
FatI CATG 4 cut(s) 28, 480, 501, 606
FauI CCCGC 1 cut(s) 671
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 3 cut(s) 111, 137, 626
Fsp4HI GCNGC 3 cut(s) 111, 137, 626
FspBI CTAG 3 cut(s) 288, 473, 671
GluI GCNGC 3 cut(s) 111, 137, 626
HaeIII GGCC 3 cut(s) 67, 269, 775
HapII CCGG 2 cut(s) 265, 723
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 4 cut(s) 32, 484, 505, 610
HinfI GANTC 3 cut(s) 169, 232, 463
HpaII CCGG 2 cut(s) 265, 723
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 83, 414
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 5 cut(s) 53, 404, 437, 614, 707
Hpy8I GTNNAC 2 cut(s) 83, 414
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 80, 93
HpyCH4IV ACGT 2 cut(s) 400, 441
HpyCH4V TGCA 8 cut(s) 76, 110, 131, 136, 151, 221, 364, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 511
HpyF3I CTNAG 2 cut(s) 165, 459
HpySE526I ACGT 2 cut(s) 400, 441
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 4 cut(s) 32, 484, 505, 610
Kzo9I GATC 2 cut(s) 58, 325
Lsp1109I GCAGC 3 cut(s) 122, 148, 637
LweI GCATC 1 cut(s) 208
MaeI CTAG 3 cut(s) 288, 473, 671
MaeII ACGT 2 cut(s) 400, 441
MaeIII GTNAC 1 cut(s) 442
MalI GATC 2 cut(s) 60, 327
MboI GATC 2 cut(s) 58, 325
MboII GAAGA 4 cut(s) 131, 134, 191, 572
MfeI CAATTG 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 5 cut(s) 180, 209, 365, 378, 386
MnlI CCTC 9 cut(s) 33, 72, 271, 299, 349, 477, 540, 651, 695
MseI TTAA 3 cut(s) 212, 389, 790
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 2 cut(s) 265, 723
MspR9I CCNGG 2 cut(s) 266, 777
MunI CAATTG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 2 cut(s) 73, 511
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 606
NdeII GATC 2 cut(s) 58, 325
NlaIII CATG 4 cut(s) 32, 484, 505, 610
NlaIV GGNNCC 1 cut(s) 762
NmeAIII GCCGAG 2 cut(s) 43, 295
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 505
PaeI GCATGC 1 cut(s) 505
PceI AGGCCT 1 cut(s) 775
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 3 cut(s) 169, 232, 463
PkrI GCNGC 3 cut(s) 112, 138, 627
PpuMI RGGWCCY 2 cut(s) 704, 761
Psp5II RGGWCCY 2 cut(s) 704, 761
Psp6I CCWGG 1 cut(s) 775
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 5 cut(s) 83, 267, 704, 761, 779
PspPPI RGGWCCY 2 cut(s) 704, 761
PstI CTGCAG 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 3 cut(s) 212, 389, 790
SatI GCNGC 3 cut(s) 111, 137, 626
Sau3AI GATC 2 cut(s) 58, 325
Sau96I GGNCC 5 cut(s) 83, 267, 704, 761, 779
ScrFI CCNGG 2 cut(s) 266, 777
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 2 cut(s) 108, 745
SinI GGWCC 4 cut(s) 83, 704, 761, 779
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
SpeI ACTAGT 1 cut(s) 472
SphI GCATGC 1 cut(s) 505
Sse9I AATT 5 cut(s) 180, 209, 365, 378, 386
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 1 cut(s) 678
SspMI CTAG 3 cut(s) 288, 473, 671
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 2 cut(s) 264, 775
StyI CCWWGG 2 cut(s) 424, 606
TaaI ACNGT 2 cut(s) 80, 93
TaiI ACGT 2 cut(s) 403, 444
TaqI TCGA 1 cut(s) 448
TasI AATT 5 cut(s) 180, 209, 365, 378, 386
TfiI GAWTC 3 cut(s) 169, 232, 463
Tru1I TTAA 3 cut(s) 212, 389, 790
Tru9I TTAA 3 cut(s) 212, 389, 790
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 110, 136, 625
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 4 cut(s) 224, 552, 573, 659
TspGWI ACGGA 1 cut(s) 320
VpaK11BI GGWCC 4 cut(s) 83, 704, 761, 779
XapI RAATTY 1 cut(s) 378
XceI RCATGY 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 3 cut(s) 288, 473, 671
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.