MD13G1042300.v1.1

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014725
Physical Location & Seq
Forward (+)
2923892 .. 2926729
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1042300.v1.1.491

Sequence Viewer

Length: 720 bp
ATGAACAGAGGGCCGGGATCCTTGTCTATTCACCTTGCAGCTTTGGAAGAACAACTTGCTGTGCAGCATAGAGAAATGCAGCGAATTATTGCTGAGAACCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGCAATTGGCAGATGCGAAAGATGAAATTCATAGACTGGGGCAGGCTATACCTAAAATCCGGGCTGAGAAAGAGGCGCAGTCTCGGGAGTTGATTGAGAGAGGATTGAAGCTAGAAGCTGATCTCCATGCTACAGAGCCTTTAAAGGCAGAAGTTGTGCAGTTAAGAGCTGAAGTTCATAAATTAAATAATTTACGGCAGGAACTGTCTTCCCAAGTCCAAGGTCTGACACAAGACGTTACTCGGTTGCAAGTGGAGAATCGGCAACTAATTGCCATGGGGGCTGATATTGATGGGATGCGAAACGAGCTTGTCGAGACCAGGAGAGCTTATGAATTTGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTCGTTTCCATGGCTCGTGAAATAGAGAAGCTAAGAGCAGAGCAGCTGAATGCAGACAGGAGGGCACGAGGACTAGGTGGTGGGGGTTACGGAATGATGAATGGAAGTCCCGAGATGAGATATTCAGGTCGTCCGTATCGCAACGGCTATAGCACTGGTTGGGGACCCTATGATAAGCGTGGATCACGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.26

Weight (kDa)

7.98

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 12, 25, 715
AcsI RAATTY 2 cut(s) 159, 467
AcuI CTGAAG 1 cut(s) 324
AgsI TTSAA 3 cut(s) 131, 241, 500
AjnI CCWGG 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 515, 547
AluBI AGCT 8 cut(s) 41, 244, 251, 302, 442, 461, 556, 571
AluI AGCT 8 cut(s) 41, 244, 251, 302, 442, 461, 556, 571
Alw26I GTCTC 2 cut(s) 219, 443
AlwI GGATC 3 cut(s) 12, 25, 715
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 2 cut(s) 216, 635
AoxI GGCC 1 cut(s) 11
ApeKI GCWGC 4 cut(s) 38, 64, 79, 568
ApoI RAATTY 2 cut(s) 159, 467
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 11, 689
AsuC2I CCSGG 2 cut(s) 15, 194
AsuHPI GGTGA 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 216, 635
AvaII GGWCC 1 cut(s) 689
BaeGI GKGCMC 1 cut(s) 592
BamHI GGATCC 1 cut(s) 17
BauI CACGAG 2 cut(s) 540, 591
BbsI GAAGAC 1 cut(s) 333
BbvI GCAGC 4 cut(s) 50, 76, 91, 580
BccI CCATC 1 cut(s) 419
BceAI ACGGC 2 cut(s) 344, 685
BciT130I CCWGG 1 cut(s) 454
BcnI CCSGG 2 cut(s) 15, 194
BcoDI GTCTC 2 cut(s) 219, 443
BfaI CTAG 2 cut(s) 245, 599
BfmI CTRYAG 2 cut(s) 264, 673
BglI GCCNNNNNGGC 1 cut(s) 413
BisI GCNGC 4 cut(s) 39, 65, 80, 569
BlsI GCNGC 4 cut(s) 40, 66, 81, 570
Bme1390I CCNGG 3 cut(s) 15, 194, 454
Bme18I GGWCC 1 cut(s) 689
BmeT110I CYCGRG 2 cut(s) 216, 635
BmgT120I GGNCC 2 cut(s) 11, 689
BmiI GGNNCC 3 cut(s) 19, 690, 691
BmrFI CCNGG 3 cut(s) 15, 194, 454
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 136, 420
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 333
BpuMI CCSGG 2 cut(s) 15, 194
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 352, 408, 534
Bse118I RCCGGY 1 cut(s) 99
Bse1I ACTGG 2 cut(s) 174, 685
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 3 cut(s) 352, 408, 534
BseGI GGATG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 84, 189
BseNI ACTGG 2 cut(s) 174, 685
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 4 cut(s) 50, 76, 91, 580
BsgI GTGCAG 2 cut(s) 83, 311
BshFI GGCC 1 cut(s) 13
BsiHKCI CYCGRG 2 cut(s) 216, 635
BsiSI CCGG 3 cut(s) 14, 100, 193
BslFI GGGAC 2 cut(s) 618, 702
BsmAI GTCTC 2 cut(s) 219, 443
BsmFI GGGAC 2 cut(s) 618, 702
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 1 cut(s) 13
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 2 cut(s) 216, 635
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 3 cut(s) 17, 253, 707
Bsp19I CCATGG 2 cut(s) 408, 534
BspANI GGCC 1 cut(s) 13
BspCNI CTCAG 2 cut(s) 85, 190
BspLI GGNNCC 3 cut(s) 19, 690, 691
BspPI GGATC 3 cut(s) 12, 25, 715
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 99
BsrI ACTGG 2 cut(s) 174, 685
BssAI RCCGGY 1 cut(s) 99
BssECI CCNNGG 3 cut(s) 352, 408, 534
BssMI GATC 3 cut(s) 17, 253, 707
BssSI CACGAG 2 cut(s) 540, 591
BssT1I CCWWGG 3 cut(s) 352, 408, 534
Bst2BI CACGAG 2 cut(s) 540, 591
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 177, 510
BstDEI CTNAG 3 cut(s) 93, 198, 557
BstDSI CCRYGG 2 cut(s) 408, 534
BstF5I GGATG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 3 cut(s) 20, 256, 710
BstMAI GTCTC 2 cut(s) 219, 443
BstMBI GATC 3 cut(s) 17, 253, 707
BstMWI GCNNNNNNNGC 2 cut(s) 413, 439
BstNI CCWGG 1 cut(s) 454
BstSCI CCNGG 3 cut(s) 13, 192, 452
BstSFI CTRYAG 2 cut(s) 264, 673
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 4 cut(s) 50, 76, 91, 580
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 1 cut(s) 13
BtgI CCRYGG 2 cut(s) 408, 534
BtsCI GGATG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 2 cut(s) 177, 510
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 11, 689
CviAII CATG 3 cut(s) 260, 409, 535
DdeI CTNAG 3 cut(s) 93, 198, 557
DpnI GATC 3 cut(s) 19, 255, 709
DpnII GATC 3 cut(s) 17, 253, 707
DraI TTTAAA 1 cut(s) 276
Eco130I CCWWGG 3 cut(s) 352, 408, 534
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 689
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 216, 635
EcoO109I RGGNCCY 1 cut(s) 689
EcoRII CCWGG 1 cut(s) 452
EcoT14I CCWWGG 3 cut(s) 352, 408, 534
ErhI CCWWGG 3 cut(s) 352, 408, 534
FaeI CATG 3 cut(s) 263, 412, 538
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 2 cut(s) 618, 702
FatI CATG 3 cut(s) 259, 408, 534
Fnu4HI GCNGC 4 cut(s) 39, 65, 80, 569
FokI GGATG 1 cut(s) 442
Fsp4HI GCNGC 4 cut(s) 39, 65, 80, 569
FspBI CTAG 2 cut(s) 245, 599
GlaI GCGC 1 cut(s) 210
GluI GCNGC 4 cut(s) 39, 65, 80, 569
HaeIII GGCC 1 cut(s) 13
HapII CCGG 3 cut(s) 14, 100, 193
HhaI GCGC 1 cut(s) 211
Hin1II CATG 3 cut(s) 263, 412, 538
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HinfI GANTC 1 cut(s) 391
HpaII CCGG 3 cut(s) 14, 100, 193
HphI GGTGA 1 cut(s) 23
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 4 cut(s) 218, 448, 542, 635
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 6 cut(s) 38, 64, 79, 292, 382, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 439
HpyF3I CTNAG 3 cut(s) 93, 198, 557
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 3 cut(s) 263, 412, 538
HspAI GCGC 1 cut(s) 209
KflI GGGWCCC 1 cut(s) 689
Kzo9I GATC 3 cut(s) 17, 253, 707
Lsp1109I GCAGC 4 cut(s) 50, 76, 91, 580
LweI GCATC 2 cut(s) 136, 420
MaeI CTAG 2 cut(s) 245, 599
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 2 cut(s) 370, 611
MalI GATC 3 cut(s) 19, 255, 709
MboI GATC 3 cut(s) 17, 253, 707
MboII GAAGA 4 cut(s) 59, 119, 333, 500
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 8 cut(s) 84, 108, 137, 159, 314, 322, 402, 467
MnlI CCTC 6 cut(s) 199, 227, 468, 536, 579, 587
MseI TTAA 4 cut(s) 275, 296, 317, 718
MspA1I CMGCKG 1 cut(s) 571
MspI CCGG 3 cut(s) 14, 100, 193
MspR9I CCNGG 3 cut(s) 15, 194, 454
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 454
MwoI GCNNNNNNNGC 2 cut(s) 413, 439
NciI CCSGG 2 cut(s) 15, 194
NcoI CCATGG 2 cut(s) 408, 534
NdeII GATC 3 cut(s) 17, 253, 707
NlaIII CATG 3 cut(s) 263, 412, 538
NlaIV GGNNCC 3 cut(s) 19, 690, 691
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 4 cut(s) 40, 66, 81, 570
PpuMI RGGWCCY 1 cut(s) 689
Psp5II RGGWCCY 1 cut(s) 689
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 19, 690, 691
PspPI GGNCC 2 cut(s) 11, 689
PspPPI RGGWCCY 1 cut(s) 689
PstNI CAGNNNCTG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 571
SaqAI TTAA 4 cut(s) 275, 296, 317, 718
SatI GCNGC 4 cut(s) 39, 65, 80, 569
Sau3AI GATC 3 cut(s) 17, 253, 707
Sau96I GGNCC 2 cut(s) 11, 689
ScrFI CCNGG 3 cut(s) 15, 194, 454
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 2 cut(s) 136, 420
SfcI CTRYAG 2 cut(s) 264, 673
SinI GGWCC 1 cut(s) 689
Sse9I AATT 8 cut(s) 84, 108, 137, 159, 314, 322, 402, 467
SspMI CTAG 2 cut(s) 245, 599
StyD4I CCNGG 3 cut(s) 13, 192, 452
StyI CCWWGG 3 cut(s) 352, 408, 534
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 1 cut(s) 372
TaqI TCGA 1 cut(s) 447
TasI AATT 8 cut(s) 84, 108, 137, 159, 314, 322, 402, 467
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 275, 296, 317, 718
Tru9I TTAA 4 cut(s) 275, 296, 317, 718
TscAI CASTG 1 cut(s) 685
TseI GCWGC 4 cut(s) 38, 64, 79, 568
TspDTI ATGAA 7 cut(s) 17, 152, 171, 299, 480, 501, 638
TspGWI ACGGA 2 cut(s) 630, 648
TspRI CASTG 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 2 cut(s) 159, 467
XspI CTAG 2 cut(s) 245, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.