pycom13g03660

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014725
Physical Location & Seq
Forward (+)
2427518 .. 2428537
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03660.2

Sequence Viewer

Length: 720 bp
ATGAACAGAGGGCCGGGACCCTTGTCTATTCACCTTGCAGCTTTGGAAGAACAACTTGCTGTGCAGCATAGAGAAATGCAGCAAATTATTGCTGAGAACCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGCAACTGGCAGATGCGAAAGATGAAATTCATAAATTGGGGCAGGCCATACCTAAAGTCCAGGCTGAGAAAGAGGCGCAGTCTAGGGAGTTGATTGAGAGAGGATTGAAGCTAGAAGCCGATCTCCGTGCTACAGAGCCTTTAAAGGCAGAAGTTGTGCAGTTAAGAGCTGAAGTTCATAAATTAAATAATTTACGGCAGGAACTGTCTTCCCAAGTCCAAGGTCTGACACAGGACGTTACTCGGTTGCAAGCAGAGAATCGGCAACTAATTGCCATGAGGGCTGATATTGATGGGATGCGAAACGAGCTTGTCGAGACCAGGAGAGCTTATGAATTTGAGAGGAAAGCCAATGAAGAACTAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTCGTTTCCATGGCTCGTGAAATAGAGAAGCTAAGAGCAGAGCAGCTGAATGCAGACAGGAGGGCACGAGGACTAGGTGGTGGAGGTTATGGAATGATGAATGGAAGTCCCGAGATGAGATATTCAGGTCGTCCGTATCGCAGCGGCCATAGTACTGGTTGGGGACCCTATGATAAGCGTGGATCACATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.17

Weight (kDa)

7.09

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 669
AclWI GGATC 1 cut(s) 715
AcoI YGGCCR 1 cut(s) 670
AcsI RAATTY 2 cut(s) 159, 467
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 679
AgsI TTSAA 3 cut(s) 131, 241, 500
AhlI ACTAGT 1 cut(s) 493
AjnI CCWGG 2 cut(s) 192, 452
AloI GAACNNNNNNTCC 2 cut(s) 515, 547
AluBI AGCT 7 cut(s) 41, 244, 302, 442, 461, 556, 571
AluI AGCT 7 cut(s) 41, 244, 302, 442, 461, 556, 571
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 715
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 3 cut(s) 11, 177, 670
ApeKI GCWGC 5 cut(s) 38, 64, 79, 568, 666
ApoI RAATTY 2 cut(s) 159, 467
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 3 cut(s) 11, 17, 689
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 2 cut(s) 17, 689
BaeGI GKGCMC 1 cut(s) 592
BauI CACGAG 2 cut(s) 540, 591
BbsI GAAGAC 1 cut(s) 333
BbvI GCAGC 5 cut(s) 50, 76, 91, 580, 678
BccI CCATC 1 cut(s) 419
BceAI ACGGC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 2 cut(s) 194, 454
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 443
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 4 cut(s) 216, 245, 494, 599
BfmI CTRYAG 1 cut(s) 264
BglI GCCNNNNNGGC 1 cut(s) 413
BisI GCNGC 6 cut(s) 39, 65, 80, 569, 667, 670
BlsI GCNGC 6 cut(s) 40, 66, 81, 570, 668, 671
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 3 cut(s) 15, 194, 454
Bme18I GGWCC 2 cut(s) 17, 689
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 3 cut(s) 11, 17, 689
BmiI GGNNCC 4 cut(s) 18, 19, 690, 691
BmrFI CCNGG 3 cut(s) 15, 194, 454
BmsI GCATC 2 cut(s) 136, 420
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 352, 534
Bse118I RCCGGY 1 cut(s) 99
Bse1I ACTGG 2 cut(s) 144, 685
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 2 cut(s) 194, 454
BseDI CCNNGG 2 cut(s) 352, 534
BseGI GGATG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 84, 189
BseNI ACTGG 2 cut(s) 144, 685
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 5 cut(s) 50, 76, 91, 580, 678
BsgI GTGCAG 2 cut(s) 83, 311
BshFI GGCC 3 cut(s) 13, 179, 672
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 2 cut(s) 14, 100
BslFI GGGAC 3 cut(s) 30, 618, 702
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 3 cut(s) 30, 618, 702
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 3 cut(s) 13, 179, 672
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 2 cut(s) 253, 707
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 1 cut(s) 669
BspANI GGCC 3 cut(s) 13, 179, 672
BspCNI CTCAG 2 cut(s) 85, 190
BspLI GGNNCC 4 cut(s) 18, 19, 690, 691
BspPI GGATC 1 cut(s) 715
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 99
BsrI ACTGG 2 cut(s) 144, 685
BssAI RCCGGY 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 352, 534
BssMI GATC 2 cut(s) 253, 707
BssSI CACGAG 2 cut(s) 540, 591
BssT1I CCWWGG 2 cut(s) 352, 534
Bst2BI CACGAG 2 cut(s) 540, 591
Bst2UI CCWGG 2 cut(s) 194, 454
Bst4CI ACNGT 1 cut(s) 339
BstC8I GCNNGC 3 cut(s) 177, 384, 510
BstDEI CTNAG 3 cut(s) 93, 198, 557
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 2 cut(s) 256, 710
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 253, 707
BstMWI GCNNNNNNNGC 2 cut(s) 413, 439
BstNI CCWGG 2 cut(s) 194, 454
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 3 cut(s) 13, 192, 452
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 5 cut(s) 50, 76, 91, 580, 678
BstV2I GAAGAC 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 680
BsuRI GGCC 3 cut(s) 13, 179, 672
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 1 cut(s) 435
Cac8I GCNNGC 3 cut(s) 177, 384, 510
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 11, 17, 689
Csp6I GTAC 1 cut(s) 678
CviAII CATG 2 cut(s) 409, 535
CviQI GTAC 1 cut(s) 678
DdeI CTNAG 3 cut(s) 93, 198, 557
DpnI GATC 2 cut(s) 255, 709
DpnII GATC 2 cut(s) 253, 707
DraI TTTAAA 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 670
Eco130I CCWWGG 2 cut(s) 352, 534
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 17, 689
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 635
EcoO109I RGGNCCY 2 cut(s) 17, 689
EcoRII CCWGG 2 cut(s) 192, 452
EcoT14I CCWWGG 2 cut(s) 352, 534
ErhI CCWWGG 2 cut(s) 352, 534
FaeI CATG 2 cut(s) 412, 538
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 3 cut(s) 30, 618, 702
FatI CATG 2 cut(s) 408, 534
Fnu4HI GCNGC 6 cut(s) 39, 65, 80, 569, 667, 670
FokI GGATG 1 cut(s) 442
Fsp4HI GCNGC 6 cut(s) 39, 65, 80, 569, 667, 670
FspBI CTAG 4 cut(s) 216, 245, 494, 599
GlaI GCGC 1 cut(s) 210
GluI GCNGC 6 cut(s) 39, 65, 80, 569, 667, 670
HaeIII GGCC 3 cut(s) 13, 179, 672
HapII CCGG 2 cut(s) 14, 100
HhaI GCGC 1 cut(s) 211
Hin1II CATG 2 cut(s) 412, 538
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HinfI GANTC 1 cut(s) 391
HpaII CCGG 2 cut(s) 14, 100
HphI GGTGA 1 cut(s) 23
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 3 cut(s) 448, 542, 635
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 6 cut(s) 38, 64, 79, 292, 382, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 439
HpyF3I CTNAG 3 cut(s) 93, 198, 557
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 2 cut(s) 412, 538
HspAI GCGC 1 cut(s) 209
KflI GGGWCCC 2 cut(s) 17, 689
Kzo9I GATC 2 cut(s) 253, 707
Lsp1109I GCAGC 5 cut(s) 50, 76, 91, 580, 678
LweI GCATC 2 cut(s) 136, 420
MaeI CTAG 4 cut(s) 216, 245, 494, 599
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 255, 709
MboI GATC 2 cut(s) 253, 707
MboII GAAGA 4 cut(s) 59, 119, 333, 500
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 8 cut(s) 84, 108, 159, 167, 314, 322, 402, 467
MnlI CCTC 8 cut(s) 199, 227, 405, 468, 536, 579, 587, 602
MseI TTAA 4 cut(s) 275, 296, 317, 718
MspA1I CMGCKG 2 cut(s) 571, 669
MspI CCGG 2 cut(s) 14, 100
MspR9I CCNGG 3 cut(s) 15, 194, 454
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 2 cut(s) 194, 454
MwoI GCNNNNNNNGC 2 cut(s) 413, 439
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 534
NdeII GATC 2 cut(s) 253, 707
NlaIII CATG 2 cut(s) 412, 538
NlaIV GGNNCC 4 cut(s) 18, 19, 690, 691
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 6 cut(s) 40, 66, 81, 570, 668, 671
PpuMI RGGWCCY 2 cut(s) 17, 689
PshAI GACNNNNGTC 1 cut(s) 22
Psp5II RGGWCCY 2 cut(s) 17, 689
Psp6I CCWGG 2 cut(s) 192, 452
PspGI CCWGG 2 cut(s) 192, 452
PspN4I GGNNCC 4 cut(s) 18, 19, 690, 691
PspPI GGNCC 3 cut(s) 11, 17, 689
PspPPI RGGWCCY 2 cut(s) 17, 689
PstNI CAGNNNCTG 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 1 cut(s) 679
RsaNI GTAC 1 cut(s) 678
SaqAI TTAA 4 cut(s) 275, 296, 317, 718
SatI GCNGC 6 cut(s) 39, 65, 80, 569, 667, 670
Sau3AI GATC 2 cut(s) 253, 707
Sau96I GGNCC 3 cut(s) 11, 17, 689
ScaI AGTACT 1 cut(s) 679
ScrFI CCNGG 3 cut(s) 15, 194, 454
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 2 cut(s) 136, 420
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 2 cut(s) 17, 689
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 8 cut(s) 84, 108, 159, 167, 314, 322, 402, 467
SsiI CCGC 1 cut(s) 669
SspMI CTAG 4 cut(s) 216, 245, 494, 599
StyD4I CCNGG 3 cut(s) 13, 192, 452
StyI CCWWGG 2 cut(s) 352, 534
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 1 cut(s) 372
TaqI TCGA 1 cut(s) 447
TasI AATT 8 cut(s) 84, 108, 159, 167, 314, 322, 402, 467
TatI WGTACW 1 cut(s) 677
TauI GCSGC 1 cut(s) 672
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 275, 296, 317, 718
Tru9I TTAA 4 cut(s) 275, 296, 317, 718
TseI GCWGC 5 cut(s) 38, 64, 79, 568, 666
TspDTI ATGAA 7 cut(s) 17, 152, 171, 299, 480, 501, 638
TspGWI ACGGA 2 cut(s) 248, 648
VpaK11BI GGWCC 2 cut(s) 17, 689
XapI RAATTY 2 cut(s) 159, 467
XspI CTAG 4 cut(s) 216, 245, 494, 599
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.