Rh4DG341300

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014725
Physical Location & Seq
Forward (+)
56346075 .. 56352309
6235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG341300.1

Sequence Viewer

Length: 1368 bp
ATGACGTCCCAATGCTCATGCGCCGCCAAGGAGGTCAAGGACTACGGTACTGCCAAGGCCATCGAAGGCGAATTCAAGCCCGTCCAGTTGTTCAGCTTTCCCAGTGTCGTTAACATTTGGAGCAAATTGCTGGAAAGTAATCCAACTATGTTTCAAAATAGTAAAACTGCTGAATCAGCTTTTGGTGCAGCAATCAAGGTATGCTCGGATAGAATGGGACAATTTGGCATGAGAGAGGTTGTGAGAGATATCCAAGGAAAGTCTTTTATTCTCTATGGCACTACTTCAAAAGTCTCGTTTGTAGCTGCTGATGTTGATGGCAATGTTCCTTTCTTCTGGGGAATTGATTTTGAAGGCAATCTTGTTATTGCTGATGATGCAGAAGTTGTGAAGGACCGTGGGAAGTTGTTTGCACCATTTCCTAAAGGGAGCTTATCAACAGAACGGAAACTTAGAGAAAATGATAGAGTGGTGGCTGAAGGAAAGATATCATCAACAAACCGAACCAAAGCATCGTTCCCGTTGAAGATCGAAGCTTTTTCAGGCAGTCACTCCTTCTCTTCTCTGATCACTATTATGGCAGGGAGAAATCGTATGCCTCGCCATGCTGATGGCTTTCGAGGTTATCGTGATGATCCTCGGCCTGTTATGCACCGTGGACCACAACCGTTTTCTATTCACCCTGCAGCTTTGGAAGAAGAACTTGCAATGCAGCACAGAGAAATGCAACGAATAATCGCTGAGAATCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGGAATTAACAGATGCAAAAGATGAGATTCATAGATTAGGGCAGGTCATACCCAAACTCCGGGCTGAGAAAGAGGCAGAGACTAGGGAGTTGATTGAGAGAGGATTAAAACTTGAAGCTGATCTCCGTGCTGTTGAGCCTGTAGGGGCAGAGGTTTTGCAATTGAGAGCTGAAATTCAGAAATTAAATGCTTTACGGCAGGAGTTGTCTACCCAAGTCCAAGGTCTCACTCAAGACGTCACTCGATTACAAGCTGAGAATCAGCAACTAGTTGCCATGAGGGCTGATATTGATGGCATGCAAAAAGAGCTTGTTGAGACAAGGAGAGCTTATGAATATGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTGATTTCCATGGCTCGTGAAATAGAGCAGCTACGAGCAGAGCAGATGAATGCAGGCAGGAGGGCACGGGGGCTAGGTGGCGGGGGTTATGGAATGATGAGTGGAGGTCCTGAAATGAGATATTCCGGTGGTTCGTATGGCAATGTCTATAGAAATGGTTGGGGACCTTATGACAGGCCTGGACCACGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

51.05

Weight (kDa)

8.26

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 64 - 147 4.6e-20 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 1020
Acc36I ACCTGC 1 cut(s) 814
AccI GTMKAC 1 cut(s) 989
AciI CCGC 2 cut(s) 24, 1254
AclWI GGATC 1 cut(s) 629
AcsI RAATTY 2 cut(s) 71, 954
AcuI CTGAAG 1 cut(s) 498
AcyI GRCGYC 2 cut(s) 5, 1017
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 840
AgsI TTSAA 8 cut(s) 76, 155, 288, 353, 526, 779, 896, 1148
AhlI ACTAGT 1 cut(s) 1048
AjnI CCWGG 1 cut(s) 1351
AjuI GAANNNNNNNTTGG 4 cut(s) 165, 197, 500, 532
AloI GAACNNNNNNTCC 2 cut(s) 1163, 1195
Alw26I GTCTC 4 cut(s) 298, 854, 1010, 1091
AlwI GGATC 1 cut(s) 629
AoxI GGCC 3 cut(s) 57, 641, 1349
ApeKI GCWGC 5 cut(s) 188, 305, 686, 712, 1201
ApoI RAATTY 2 cut(s) 71, 954
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 5 cut(s) 394, 659, 1280, 1337, 1355
AsuC2I CCSGG 1 cut(s) 842
AsuHPI GGTGA 1 cut(s) 671
AvaII GGWCC 5 cut(s) 394, 659, 1280, 1337, 1355
BaeGI GKGCMC 1 cut(s) 1240
BauI CACGAG 1 cut(s) 1188
BbvI GCAGC 5 cut(s) 200, 292, 698, 724, 1213
BccI CCATC 4 cut(s) 68, 311, 605, 1067
BceAI ACGGC 1 cut(s) 992
BciT130I CCWGG 1 cut(s) 1353
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 1 cut(s) 842
BcoDI GTCTC 4 cut(s) 298, 854, 1010, 1091
BcuI ACTAGT 1 cut(s) 1048
BfaI CTAG 3 cut(s) 864, 1049, 1247
BfmI CTRYAG 3 cut(s) 684, 921, 1321
BfuAI ACCTGC 1 cut(s) 814
BglI GCCNNNNNGGC 1 cut(s) 1061
BisI GCNGC 6 cut(s) 24, 189, 306, 687, 713, 1202
BlsI GCNGC 6 cut(s) 25, 190, 307, 688, 714, 1203
Bme1390I CCNGG 2 cut(s) 842, 1353
Bme18I GGWCC 5 cut(s) 394, 659, 1280, 1337, 1355
BmgT120I GGNCC 5 cut(s) 394, 659, 1280, 1337, 1355
BmiI GGNNCC 1 cut(s) 1338
BmrFI CCNGG 2 cut(s) 842, 1353
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 3 cut(s) 367, 521, 784
BmuI ACTGGG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 996
BpuMI CCSGG 1 cut(s) 842
BsaHI GRCGYC 2 cut(s) 5, 1017
BsaI GGTCTC 1 cut(s) 1010
BsaJI CCNNGG 9 cut(s) 27, 54, 253, 397, 638, 655, 1000, 1182, 1358
BsaWI WCCGGW 1 cut(s) 1298
BsaXI ACNNNNNCTCC 4 cut(s) 577, 607, 822, 852
Bsc4I CCNNNNNNNGG 1 cut(s) 840
Bse1I ACTGG 2 cut(s) 85, 102
Bse3DI GCAATG 4 cut(s) 328, 714, 1167, 1321
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 9 cut(s) 27, 54, 253, 397, 638, 655, 1000, 1182, 1358
BseLI CCNNNNNNNGG 1 cut(s) 840
BseMI GCAATG 4 cut(s) 328, 714, 1167, 1321
BseMII CTCAG 3 cut(s) 732, 837, 1026
BseNI ACTGG 2 cut(s) 85, 102
BseSI GKGCMC 1 cut(s) 1240
BseXI GCAGC 5 cut(s) 200, 292, 698, 724, 1213
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 3 cut(s) 59, 643, 1351
BsiSI CCGG 2 cut(s) 841, 1299
BslFI GGGAC 2 cut(s) 231, 1350
BslI CCNNNNNNNGG 1 cut(s) 840
BsmAI GTCTC 4 cut(s) 298, 854, 1010, 1091
BsmFI GGGAC 2 cut(s) 231, 1350
BsmI GAATGC 1 cut(s) 1228
BsnI GGCC 3 cut(s) 59, 643, 1351
Bso31I GGTCTC 1 cut(s) 1010
Bsp1286I GDGCHC 1 cut(s) 1240
Bsp143I GATC 4 cut(s) 528, 567, 634, 901
Bsp19I CCATGG 1 cut(s) 1182
BspACI CCGC 2 cut(s) 24, 1254
BspANI GGCC 3 cut(s) 59, 643, 1351
BspCNI CTCAG 3 cut(s) 733, 838, 1027
BspLI GGNNCC 1 cut(s) 1338
BspMAI CTGCAG 1 cut(s) 688
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 1010
BsrDI GCAATG 4 cut(s) 328, 714, 1167, 1321
BsrI ACTGG 2 cut(s) 85, 102
BssECI CCNNGG 9 cut(s) 27, 54, 253, 397, 638, 655, 1000, 1182, 1358
BssMI GATC 4 cut(s) 528, 567, 634, 901
BssNI GRCGYC 2 cut(s) 5, 1017
BssSI CACGAG 1 cut(s) 1188
BssT1I CCWWGG 5 cut(s) 27, 54, 253, 1000, 1182
Bst2BI CACGAG 1 cut(s) 1188
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 4 cut(s) 47, 398, 656, 669
Bst6I CTCTTC 1 cut(s) 565
BstACI GRCGYC 2 cut(s) 5, 1017
BstC8I GCNNGC 3 cut(s) 1079, 1158, 1228
BstDEI CTNAG 4 cut(s) 452, 741, 846, 1035
BstDSI CCRYGG 4 cut(s) 397, 655, 1182, 1358
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 4 cut(s) 531, 570, 637, 904
BstMAI GTCTC 4 cut(s) 298, 854, 1010, 1091
BstMBI GATC 4 cut(s) 528, 567, 634, 901
BstMWI GCNNNNNNNGC 6 cut(s) 176, 185, 377, 649, 1061, 1087
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 2 cut(s) 840, 1351
BstSFI CTRYAG 3 cut(s) 684, 921, 1321
BstSLI GKGCMC 1 cut(s) 1240
BstV1I GCAGC 5 cut(s) 200, 292, 698, 724, 1213
BstXI CCANNNNNNTGG 1 cut(s) 611
BsuRI GGCC 3 cut(s) 59, 643, 1351
BtgI CCRYGG 4 cut(s) 397, 655, 1182, 1358
BtsIMutI CAGTG 1 cut(s) 109
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 3 cut(s) 1079, 1158, 1228
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 5 cut(s) 394, 659, 1280, 1337, 1355
Csp6I GTAC 1 cut(s) 48
CviAII CATG 6 cut(s) 18, 229, 605, 1057, 1078, 1183
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 4 cut(s) 452, 741, 846, 1035
DpnI GATC 4 cut(s) 530, 569, 636, 903
DpnII GATC 4 cut(s) 528, 567, 634, 901
Eam1104I CTCTTC 1 cut(s) 565
EarI CTCTTC 1 cut(s) 565
Eco130I CCWWGG 5 cut(s) 27, 54, 253, 1000, 1182
Eco147I AGGCCT 1 cut(s) 1351
Eco31I GGTCTC 1 cut(s) 1010
Eco32I GATATC 2 cut(s) 250, 489
Eco47I GGWCC 5 cut(s) 394, 659, 1280, 1337, 1355
Eco57I CTGAAG 1 cut(s) 498
EcoO109I RGGNCCY 2 cut(s) 1280, 1337
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 1351
EcoRV GATATC 2 cut(s) 250, 489
EcoT14I CCWWGG 5 cut(s) 27, 54, 253, 1000, 1182
ErhI CCWWGG 5 cut(s) 27, 54, 253, 1000, 1182
FaeI CATG 6 cut(s) 21, 232, 608, 1060, 1081, 1186
FalI AAGNNNNNCTT 6 cut(s) 345, 377, 687, 719, 1093, 1125
FaqI GGGAC 2 cut(s) 231, 1350
FatI CATG 6 cut(s) 17, 228, 604, 1056, 1077, 1182
FauI CCCGC 1 cut(s) 1247
FbaI TGATCA 1 cut(s) 567
FblI GTMKAC 1 cut(s) 989
Fnu4HI GCNGC 6 cut(s) 24, 189, 306, 687, 713, 1202
Fsp4HI GCNGC 6 cut(s) 24, 189, 306, 687, 713, 1202
FspBI CTAG 3 cut(s) 864, 1049, 1247
GlaI GCGC 1 cut(s) 22
GluI GCNGC 6 cut(s) 24, 189, 306, 687, 713, 1202
HaeIII GGCC 3 cut(s) 59, 643, 1351
HapII CCGG 2 cut(s) 841, 1299
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 2 cut(s) 5, 1017
Hin1II CATG 6 cut(s) 21, 232, 608, 1060, 1081, 1186
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 4 cut(s) 173, 745, 808, 1039
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 2 cut(s) 841, 1299
HphI GGTGA 1 cut(s) 671
Hpy166II GTNNAC 3 cut(s) 112, 659, 990
Hpy188I TCNGA 3 cut(s) 208, 567, 960
Hpy188III TCNNGA 4 cut(s) 629, 1013, 1190, 1283
Hpy8I GTNNAC 3 cut(s) 112, 659, 990
HpyAV CCTTC 6 cut(s) 59, 347, 385, 473, 565, 782
HpyCH4III ACNGT 4 cut(s) 47, 398, 656, 669
HpyCH4IV ACGT 2 cut(s) 5, 1017
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 185, 377, 649, 1061, 1087
HpyF3I CTNAG 4 cut(s) 452, 741, 846, 1035
HpySE526I ACGT 2 cut(s) 5, 1017
Hsp92I GRCGYC 2 cut(s) 5, 1017
Hsp92II CATG 6 cut(s) 21, 232, 608, 1060, 1081, 1186
HspAI GCGC 1 cut(s) 21
Ksp22I TGATCA 1 cut(s) 567
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 4 cut(s) 528, 567, 634, 901
LmnI GCTCC 2 cut(s) 120, 429
Lsp1109I GCAGC 5 cut(s) 200, 292, 698, 724, 1213
LweI GCATC 3 cut(s) 367, 521, 784
MaeI CTAG 3 cut(s) 864, 1049, 1247
MaeII ACGT 2 cut(s) 5, 1017
MaeIII GTNAC 2 cut(s) 548, 1018
MalI GATC 4 cut(s) 530, 569, 636, 903
MboI GATC 4 cut(s) 528, 567, 634, 901
MboII GAAGA 7 cut(s) 325, 538, 552, 707, 710, 767, 1148
MfeI CAATTG 1 cut(s) 941
MhlI GDGCHC 1 cut(s) 1240
MluCI AATT 9 cut(s) 71, 125, 221, 342, 756, 785, 941, 954, 962
MmeI TCCRAC 1 cut(s) 167
MseI TTAA 5 cut(s) 111, 788, 887, 965, 1366
MslI CAYNNNNRTG 2 cut(s) 575, 609
MspI CCGG 2 cut(s) 841, 1299
MspR9I CCNGG 2 cut(s) 842, 1353
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 1 cut(s) 1228
MvaI CCWGG 1 cut(s) 1353
MwoI GCNNNNNNNGC 6 cut(s) 176, 185, 377, 649, 1061, 1087
NciI CCSGG 1 cut(s) 842
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 4 cut(s) 528, 567, 634, 901
NlaIII CATG 6 cut(s) 21, 232, 608, 1060, 1081, 1186
NlaIV GGNNCC 1 cut(s) 1338
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 2 cut(s) 548, 1018
NspI RCATGY 1 cut(s) 1081
PaeI GCATGC 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 1351
PcsI WCGNNNNNNNCGW 1 cut(s) 625
PctI GAATGC 1 cut(s) 1228
PfeI GAWTC 4 cut(s) 173, 745, 808, 1039
PkrI GCNGC 6 cut(s) 25, 190, 307, 688, 714, 1203
PpuMI RGGWCCY 2 cut(s) 1280, 1337
Psp5II RGGWCCY 2 cut(s) 1280, 1337
Psp6I CCWGG 1 cut(s) 1351
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 1 cut(s) 1338
PspPI GGNCC 5 cut(s) 394, 659, 1280, 1337, 1355
PspPPI RGGWCCY 2 cut(s) 1280, 1337
PstI CTGCAG 1 cut(s) 688
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 2 cut(s) 575, 609
SaqAI TTAA 5 cut(s) 111, 788, 887, 965, 1366
SatI GCNGC 6 cut(s) 24, 189, 306, 687, 713, 1202
Sau3AI GATC 4 cut(s) 528, 567, 634, 901
Sau96I GGNCC 5 cut(s) 394, 659, 1280, 1337, 1355
ScrFI CCNGG 2 cut(s) 842, 1353
SduI GDGCHC 1 cut(s) 1240
SfaNI GCATC 3 cut(s) 367, 521, 784
SfcI CTRYAG 3 cut(s) 684, 921, 1321
SinI GGWCC 5 cut(s) 394, 659, 1280, 1337, 1355
SmiMI CAYNNNNRTG 2 cut(s) 575, 609
SmlI CTYRAG 1 cut(s) 1011
SmoI CTYRAG 1 cut(s) 1011
SpeI ACTAGT 1 cut(s) 1048
SphI GCATGC 1 cut(s) 1081
Sse9I AATT 9 cut(s) 71, 125, 221, 342, 756, 785, 941, 954, 962
SseBI AGGCCT 1 cut(s) 1351
SsiI CCGC 2 cut(s) 24, 1254
SspMI CTAG 3 cut(s) 864, 1049, 1247
StuI AGGCCT 1 cut(s) 1351
StyD4I CCNGG 2 cut(s) 840, 1351
StyI CCWWGG 5 cut(s) 27, 54, 253, 1000, 1182
TaaI ACNGT 4 cut(s) 47, 398, 656, 669
TaiI ACGT 2 cut(s) 8, 1020
TaqI TCGA 4 cut(s) 63, 531, 619, 1024
TasI AATT 9 cut(s) 71, 125, 221, 342, 756, 785, 941, 954, 962
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 4 cut(s) 173, 745, 808, 1039
Tru1I TTAA 5 cut(s) 111, 788, 887, 965, 1366
Tru9I TTAA 5 cut(s) 111, 788, 887, 965, 1366
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 2 cut(s) 548, 1018
TseI GCWGC 5 cut(s) 188, 305, 686, 712, 1201
Tsp45I GTSAC 2 cut(s) 548, 1018
TspDTI ATGAA 4 cut(s) 800, 1128, 1149, 1235
TspGWI ACGGA 2 cut(s) 460, 896
TspRI CASTG 1 cut(s) 109
VpaK11BI GGWCC 5 cut(s) 394, 659, 1280, 1337, 1355
XapI RAATTY 2 cut(s) 71, 954
XceI RCATGY 1 cut(s) 1081
XmiI GTMKAC 1 cut(s) 989
XspI CTAG 3 cut(s) 864, 1049, 1247
ZraI GACGTC 2 cut(s) 6, 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.