pycom13g03680

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014725
Physical Location & Seq
Forward (+)
2440825 .. 2441893
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03680.1

Sequence Viewer

Length: 723 bp
ATGAACAGAGGGCTGGGACCCTTGTCTATGCACCTTGCAGCTTTGGAAGAACAACTTGCTGTGCAGCATAGAGAAATGCAGCGAATTATTGCTGAGAACCGGCTTGTAATTGAAGATAATACCCTTCTTCAAAGGCAATTGGCAGATGCGAAAGATGAAATTCATAGATTGGGGCAGGCCATACCTAAAATCCGGGCTGAGAAAGAGGCGCAGTCTCAGGAGTTGATTGAGAGAGGATTGAAGCTAGAAGCCGATCATCTCTGTGCTACAGATCCTTTAAAGGCAGAAGTTGTGCAGTTAAGAGCTGAAGTTCATAAATTAAATAATTTACGGCAGGAACTGTCTTCCCAAATCCAAGGTCTGACACAGGACGTTACTCAGTTGCAAGCAGAGAATCGGCAACTAATTGCCATGAGGGCTGATATTGATGGGATGCGAAACGAGCTTGTCGAGACCAGGAGAGCTTATGAATTTGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTTGTTTCCATGGCTCGTGAAATAGAGAAGCTAAGAGCAGAGCAGCTGAATGCAGACAGGAGGGCACGAGGACTAGGTGGTGGGGGTTATGGAATGATGAATGGAAGTCCCGAGATGAGATATTCAGGGCGTCCGTATCGCAGCGGCCATAGCACTGGTTGGGGACCCTATGATAAGCGTGGATCACCGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

27.32

Weight (kDa)

6.68

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 672, 716
AclWI GGATC 2 cut(s) 266, 718
AcoI YGGCCR 1 cut(s) 673
AcsI RAATTY 2 cut(s) 159, 470
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 658
AgsI TTSAA 4 cut(s) 113, 131, 241, 503
AjnI CCWGG 1 cut(s) 455
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 7 cut(s) 41, 244, 305, 445, 464, 559, 574
AluI AGCT 7 cut(s) 41, 244, 305, 445, 464, 559, 574
Alw26I GTCTC 2 cut(s) 219, 446
AlwI GGATC 2 cut(s) 266, 718
AlwNI CAGNNNCTG 1 cut(s) 340
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 2 cut(s) 177, 673
ApeKI GCWGC 5 cut(s) 38, 64, 79, 571, 669
ApoI RAATTY 2 cut(s) 159, 470
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 17, 692
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 2 cut(s) 17, 692
BaeGI GKGCMC 1 cut(s) 595
BauI CACGAG 2 cut(s) 543, 594
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 5 cut(s) 50, 76, 91, 583, 681
BccI CCATC 1 cut(s) 422
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 1 cut(s) 457
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 219, 446
BfaI CTAG 2 cut(s) 245, 602
BfmI CTRYAG 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 416
BisI GCNGC 6 cut(s) 39, 65, 80, 572, 670, 673
BlsI GCNGC 6 cut(s) 40, 66, 81, 573, 671, 674
Bme1390I CCNGG 2 cut(s) 194, 457
Bme18I GGWCC 2 cut(s) 17, 692
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 2 cut(s) 17, 692
BmiI GGNNCC 4 cut(s) 18, 19, 693, 694
BmrFI CCNGG 2 cut(s) 194, 457
BmsI GCATC 2 cut(s) 136, 423
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 658
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 355, 537
Bse118I RCCGGY 1 cut(s) 99
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 2 cut(s) 355, 537
BseGI GGATG 1 cut(s) 438
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 4 cut(s) 84, 189, 230, 392
BseNI ACTGG 1 cut(s) 688
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 5 cut(s) 50, 76, 91, 583, 681
BseYI CCCAGC 1 cut(s) 13
BsgI GTGCAG 2 cut(s) 83, 314
BshFI GGCC 2 cut(s) 179, 675
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 2 cut(s) 100, 193
BslFI GGGAC 3 cut(s) 30, 621, 705
BsmAI GTCTC 2 cut(s) 219, 446
BsmFI GGGAC 3 cut(s) 30, 621, 705
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 2 cut(s) 179, 675
Bso31I GGTCTC 1 cut(s) 446
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 3 cut(s) 253, 271, 710
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 2 cut(s) 672, 716
BspANI GGCC 2 cut(s) 179, 675
BspCNI CTCAG 4 cut(s) 85, 190, 229, 391
BspLI GGNNCC 4 cut(s) 18, 19, 693, 694
BspPI GGATC 2 cut(s) 266, 718
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 1 cut(s) 99
BsrI ACTGG 1 cut(s) 688
BssAI RCCGGY 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 355, 537
BssMI GATC 3 cut(s) 253, 271, 710
BssNI GRCGYC 1 cut(s) 658
BssSI CACGAG 2 cut(s) 543, 594
BssT1I CCWWGG 2 cut(s) 355, 537
Bst2BI CACGAG 2 cut(s) 543, 594
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 658
BstC8I GCNNGC 3 cut(s) 177, 387, 513
BstDEI CTNAG 5 cut(s) 93, 198, 216, 378, 560
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 3 cut(s) 256, 274, 713
BstMAI GTCTC 2 cut(s) 219, 446
BstMBI GATC 3 cut(s) 253, 271, 710
BstMWI GCNNNNNNNGC 3 cut(s) 416, 442, 678
BstNI CCWGG 1 cut(s) 457
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 192, 455
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 595
BstV1I GCAGC 5 cut(s) 50, 76, 91, 583, 681
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 2 cut(s) 179, 675
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 1 cut(s) 438
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 3 cut(s) 177, 387, 513
CaiI CAGNNNCTG 1 cut(s) 340
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 17, 692
CseI GACGC 1 cut(s) 647
CviAII CATG 2 cut(s) 412, 538
DdeI CTNAG 5 cut(s) 93, 198, 216, 378, 560
DpnI GATC 3 cut(s) 255, 273, 712
DpnII GATC 3 cut(s) 253, 271, 710
DraI TTTAAA 1 cut(s) 279
EaeI YGGCCR 1 cut(s) 673
Eco130I CCWWGG 2 cut(s) 355, 537
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 2 cut(s) 17, 692
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 638
EcoO109I RGGNCCY 2 cut(s) 17, 692
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 355, 537
ErhI CCWWGG 2 cut(s) 355, 537
FaeI CATG 2 cut(s) 415, 541
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 3 cut(s) 30, 621, 705
FatI CATG 2 cut(s) 411, 537
Fnu4HI GCNGC 6 cut(s) 39, 65, 80, 572, 670, 673
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 6 cut(s) 39, 65, 80, 572, 670, 673
FspBI CTAG 2 cut(s) 245, 602
GlaI GCGC 1 cut(s) 210
GluI GCNGC 6 cut(s) 39, 65, 80, 572, 670, 673
GsaI CCCAGC 1 cut(s) 17
HaeIII GGCC 2 cut(s) 179, 675
HapII CCGG 2 cut(s) 100, 193
HgaI GACGC 1 cut(s) 647
HhaI GCGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 658
Hin1II CATG 2 cut(s) 415, 541
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HinfI GANTC 1 cut(s) 394
HpaII CCGG 2 cut(s) 100, 193
HphI GGTGA 1 cut(s) 705
Hpy188I TCNGA 1 cut(s) 363
Hpy188III TCNNGA 4 cut(s) 218, 451, 545, 638
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 31, 38, 64, 79, 295, 385, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 442, 678
HpyF3I CTNAG 5 cut(s) 93, 198, 216, 378, 560
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 658
Hsp92II CATG 2 cut(s) 415, 541
HspAI GCGC 1 cut(s) 209
KflI GGGWCCC 2 cut(s) 17, 692
Kzo9I GATC 3 cut(s) 253, 271, 710
Lsp1109I GCAGC 5 cut(s) 50, 76, 91, 583, 681
LweI GCATC 2 cut(s) 136, 423
MaeI CTAG 2 cut(s) 245, 602
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 373
MalI GATC 3 cut(s) 255, 273, 712
MboI GATC 3 cut(s) 253, 271, 710
MboII GAAGA 5 cut(s) 59, 119, 125, 336, 503
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 8 cut(s) 84, 108, 137, 159, 317, 325, 405, 470
MnlI CCTC 6 cut(s) 199, 227, 408, 471, 582, 590
MseI TTAA 4 cut(s) 278, 299, 320, 721
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 574, 672
MspI CCGG 2 cut(s) 100, 193
MspR9I CCNGG 2 cut(s) 194, 457
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 1 cut(s) 457
MwoI GCNNNNNNNGC 3 cut(s) 416, 442, 678
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 537
NdeII GATC 3 cut(s) 253, 271, 710
NlaIII CATG 2 cut(s) 415, 541
NlaIV GGNNCC 4 cut(s) 18, 19, 693, 694
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 1 cut(s) 583
PfeI GAWTC 1 cut(s) 394
PkrI GCNGC 6 cut(s) 40, 66, 81, 573, 671, 674
PpuMI RGGWCCY 2 cut(s) 17, 692
PshAI GACNNNNGTC 1 cut(s) 22
Psp5II RGGWCCY 2 cut(s) 17, 692
Psp6I CCWGG 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 4 cut(s) 18, 19, 693, 694
PspPI GGNCC 2 cut(s) 17, 692
PspPPI RGGWCCY 2 cut(s) 17, 692
PstNI CAGNNNCTG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 574
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 4 cut(s) 278, 299, 320, 721
SatI GCNGC 6 cut(s) 39, 65, 80, 572, 670, 673
Sau3AI GATC 3 cut(s) 253, 271, 710
Sau96I GGNCC 2 cut(s) 17, 692
ScrFI CCNGG 2 cut(s) 194, 457
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 2 cut(s) 136, 423
SfcI CTRYAG 1 cut(s) 267
SinI GGWCC 2 cut(s) 17, 692
SmiMI CAYNNNNRTG 1 cut(s) 261
Sse9I AATT 8 cut(s) 84, 108, 137, 159, 317, 325, 405, 470
SsiI CCGC 2 cut(s) 672, 716
SspMI CTAG 2 cut(s) 245, 602
StyD4I CCNGG 2 cut(s) 192, 455
StyI CCWWGG 2 cut(s) 355, 537
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 450
TasI AATT 8 cut(s) 84, 108, 137, 159, 317, 325, 405, 470
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 4 cut(s) 278, 299, 320, 721
Tru9I TTAA 4 cut(s) 278, 299, 320, 721
TscAI CASTG 1 cut(s) 688
TseI GCWGC 5 cut(s) 38, 64, 79, 571, 669
TspDTI ATGAA 7 cut(s) 17, 152, 171, 302, 483, 504, 641
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 1 cut(s) 688
VpaK11BI GGWCC 2 cut(s) 17, 692
XapI RAATTY 2 cut(s) 159, 470
XspI CTAG 2 cut(s) 245, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.