MD16G1043100.v1.1

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014725
Physical Location & Seq
Forward (+)
3039057 .. 3041455
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1043100.v1.1.491

Sequence Viewer

Length: 774 bp
ATGCCTCGCCATTCTGATGGTTATCGAGGTTATCGTGATGTTCCGCGACCTGTTATGAACCGAGGACCGGGACCCTTGTCCATTCGCCCTGCAGCTCTGGAAGAAGAACTTGCTGTGCAGCGTAGAGAAATGCAGCGAATTATTGCTGAGAACCAGCTTGTAATTGATGATAATACCCTTCTTCAAAGGCAATTGACAGATGCGAGAGATGAAATTCATAGATTGGGGCAGCTCATACCTAAAATCCGGGCTGAGAAAGAGGCGCAGTCTAGGGAGCTGATTGAGAGAGAATTGAAGCTAGAAGCTGATCTCCGTGCTACAGAGCCTTTAAAGGCAGAAGTTATGCAGTTAAGAGCTGAAGTTCATAAACTAAATAATTTACGGCAGGAACTGTCTTCCCAAGTCCAAGGTTTGACACAGGATGTTACTCGGTTGCAATCGGAGAATCAGCAACTAATTGCCATGAGGGCTGATATTGATGTGATGCGGAACGAGCTTGTTGAGACCAGGAGAGCTTATGAATTTGAGAGGAAAGCCAATGAAGAACAAGTTGAGCAAAAGCAAGCAATGGAAAAGAATCTCGTCTCCATGGCTCGTGATATAGAGAAGCTAAGAGCAGAGCAGCTGAATGCAGACAGGAGGGCACAAGGACTAGGTGGTAGGAGTTACGGAATGATGAATGAAAGTCCCGATATGAGATATTCAGGTGGACCATATCGCAATGGATATAGCACCGGTTGGGGACCCTATGACAGGCGTGGATCACGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

29.8

Weight (kDa)

7.95

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 46
AciI CCGC 2 cut(s) 44, 487
AclWI GGATC 1 cut(s) 769
AcsI RAATTY 2 cut(s) 213, 521
AcuI CTGAAG 1 cut(s) 378
AfiI CCNNNNNNNGG 2 cut(s) 67, 753
AgeI ACCGGT 1 cut(s) 734
AgsI TTSAA 2 cut(s) 185, 295
AjnI CCWGG 1 cut(s) 506
Alw26I GTCTC 2 cut(s) 497, 589
AlwI GGATC 1 cut(s) 769
AlwNI CAGNNNCTG 1 cut(s) 391
ApeKI GCWGC 5 cut(s) 92, 118, 133, 229, 622
ApoI RAATTY 2 cut(s) 213, 521
AsiGI ACCGGT 1 cut(s) 734
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 4 cut(s) 65, 71, 710, 743
AsuC2I CCSGG 2 cut(s) 69, 248
AvaII GGWCC 4 cut(s) 65, 71, 710, 743
BaeGI GKGCMC 1 cut(s) 646
BauI CACGAG 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 5 cut(s) 104, 130, 145, 241, 634
BccI CCATC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 508
BcnI CCSGG 2 cut(s) 69, 248
BcoDI GTCTC 2 cut(s) 497, 589
BfaI CTAG 3 cut(s) 270, 299, 653
BfmI CTRYAG 2 cut(s) 90, 318
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 5 cut(s) 93, 119, 134, 230, 623
BlsI GCNGC 5 cut(s) 94, 120, 135, 231, 624
Bme1390I CCNGG 3 cut(s) 69, 248, 508
Bme18I GGWCC 4 cut(s) 65, 71, 710, 743
BmgT120I GGNCC 4 cut(s) 65, 71, 710, 743
BmiI GGNNCC 4 cut(s) 72, 73, 744, 745
BmrFI CCNGG 3 cut(s) 69, 248, 508
BmsI GCATC 2 cut(s) 190, 474
BoxI GACNNNNGTC 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 387
BpuMI CCSGG 2 cut(s) 69, 248
BsaBI GATNNNNATC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 61, 406, 588
BsaWI WCCGGW 1 cut(s) 734
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 753
Bse118I RCCGGY 1 cut(s) 734
Bse3DI GCAATG 2 cut(s) 573, 727
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 3 cut(s) 61, 406, 588
BseGI GGATG 1 cut(s) 427
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 67, 753
BseMI GCAATG 2 cut(s) 573, 727
BseMII CTCAG 2 cut(s) 138, 243
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 5 cut(s) 104, 130, 145, 241, 634
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 1 cut(s) 46
BshTI ACCGGT 1 cut(s) 734
BsiSI CCGG 3 cut(s) 68, 247, 735
BslFI GGGAC 3 cut(s) 84, 672, 756
BslI CCNNNNNNNGG 2 cut(s) 67, 753
BsmAI GTCTC 2 cut(s) 497, 589
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 3 cut(s) 84, 672, 756
BsmI GAATGC 1 cut(s) 634
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 2 cut(s) 307, 761
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 2 cut(s) 44, 487
BspCNI CTCAG 2 cut(s) 139, 244
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 4 cut(s) 72, 73, 744, 745
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 1 cut(s) 769
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 2 cut(s) 573, 727
BsrFI RCCGGY 1 cut(s) 734
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 3 cut(s) 61, 406, 588
BssMI GATC 2 cut(s) 307, 761
BssSI CACGAG 1 cut(s) 594
BssT1I CCWWGG 2 cut(s) 406, 588
Bst2BI CACGAG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 3 cut(s) 147, 252, 611
BstDSI CCRYGG 1 cut(s) 588
BstENI CCTNNNNNAGG 1 cut(s) 751
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 46
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 2 cut(s) 310, 764
BstMAI GTCTC 2 cut(s) 497, 589
BstMBI GATC 2 cut(s) 307, 761
BstMWI GCNNNNNNNGC 2 cut(s) 467, 493
BstNI CCWGG 1 cut(s) 508
BstPAI GACNNNNGTC 1 cut(s) 76
BstSCI CCNGG 3 cut(s) 67, 246, 506
BstSFI CTRYAG 2 cut(s) 90, 318
BstSLI GKGCMC 1 cut(s) 646
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 5 cut(s) 104, 130, 145, 241, 634
BstV2I GAAGAC 1 cut(s) 387
BstXI CCANNNNNNTGG 1 cut(s) 17
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 564
CaiI CAGNNNCTG 1 cut(s) 391
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 4 cut(s) 65, 71, 710, 743
CspAI ACCGGT 1 cut(s) 734
CviAII CATG 2 cut(s) 463, 589
DdeI CTNAG 3 cut(s) 147, 252, 611
DpnI GATC 2 cut(s) 309, 763
DpnII GATC 2 cut(s) 307, 761
DraI TTTAAA 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 406, 588
Eco31I GGTCTC 1 cut(s) 497
Eco47I GGWCC 4 cut(s) 65, 71, 710, 743
Eco57I CTGAAG 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 751
EcoO109I RGGNCCY 2 cut(s) 71, 743
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 2 cut(s) 406, 588
ErhI CCWWGG 2 cut(s) 406, 588
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 2 cut(s) 466, 592
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 3 cut(s) 84, 672, 756
FatI CATG 2 cut(s) 462, 588
Fnu4HI GCNGC 5 cut(s) 93, 119, 134, 230, 623
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 5 cut(s) 93, 119, 134, 230, 623
FspBI CTAG 3 cut(s) 270, 299, 653
GlaI GCGC 1 cut(s) 264
GluI GCNGC 5 cut(s) 93, 119, 134, 230, 623
HapII CCGG 3 cut(s) 68, 247, 735
HhaI GCGC 1 cut(s) 265
Hin1II CATG 2 cut(s) 466, 592
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 2 cut(s) 445, 577
HpaII CCGG 3 cut(s) 68, 247, 735
Hpy166II GTNNAC 1 cut(s) 710
Hpy188I TCNGA 2 cut(s) 16, 442
Hpy188III TCNNGA 5 cut(s) 35, 98, 596, 689, 765
Hpy8I GTNNAC 1 cut(s) 710
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 393
HpyCH4V TGCA 6 cut(s) 92, 118, 133, 346, 436, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 467, 493
HpyF3I CTNAG 3 cut(s) 147, 252, 611
Hsp92II CATG 2 cut(s) 466, 592
HspAI GCGC 1 cut(s) 263
KflI GGGWCCC 2 cut(s) 71, 743
Kzo9I GATC 2 cut(s) 307, 761
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 5 cut(s) 104, 130, 145, 241, 634
LweI GCATC 2 cut(s) 190, 474
MaeI CTAG 3 cut(s) 270, 299, 653
MaeIII GTNAC 2 cut(s) 424, 665
MalI GATC 2 cut(s) 309, 763
MboI GATC 2 cut(s) 307, 761
MboII GAAGA 5 cut(s) 113, 116, 173, 387, 554
MfeI CAATTG 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 8 cut(s) 138, 162, 191, 213, 290, 376, 456, 521
MnlI CCTC 7 cut(s) 15, 20, 56, 253, 459, 522, 633
MseI TTAA 3 cut(s) 329, 350, 772
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 3 cut(s) 68, 247, 735
MspR9I CCNGG 3 cut(s) 69, 248, 508
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 1 cut(s) 508
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 467, 493
NciI CCSGG 2 cut(s) 69, 248
NcoI CCATGG 1 cut(s) 588
NdeII GATC 2 cut(s) 307, 761
NlaIII CATG 2 cut(s) 466, 592
NlaIV GGNNCC 4 cut(s) 72, 73, 744, 745
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 2 cut(s) 445, 577
PinAI ACCGGT 1 cut(s) 734
PkrI GCNGC 5 cut(s) 94, 120, 135, 231, 624
PpuMI RGGWCCY 2 cut(s) 71, 743
PshAI GACNNNNGTC 1 cut(s) 76
Psp5II RGGWCCY 2 cut(s) 71, 743
Psp6I CCWGG 1 cut(s) 506
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 4 cut(s) 72, 73, 744, 745
PspPI GGNCC 4 cut(s) 65, 71, 710, 743
PspPPI RGGWCCY 2 cut(s) 71, 743
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 625
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 3 cut(s) 329, 350, 772
SatI GCNGC 5 cut(s) 93, 119, 134, 230, 623
Sau3AI GATC 2 cut(s) 307, 761
Sau96I GGNCC 4 cut(s) 65, 71, 710, 743
ScrFI CCNGG 3 cut(s) 69, 248, 508
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 2 cut(s) 190, 474
SfcI CTRYAG 2 cut(s) 90, 318
SinI GGWCC 4 cut(s) 65, 71, 710, 743
SmiMI CAYNNNNRTG 1 cut(s) 15
Sse9I AATT 8 cut(s) 138, 162, 191, 213, 290, 376, 456, 521
SsiI CCGC 2 cut(s) 44, 487
SspMI CTAG 3 cut(s) 270, 299, 653
StyD4I CCNGG 3 cut(s) 67, 246, 506
StyI CCWWGG 2 cut(s) 406, 588
TaaI ACNGT 1 cut(s) 393
TaqI TCGA 1 cut(s) 25
TasI AATT 8 cut(s) 138, 162, 191, 213, 290, 376, 456, 521
TfiI GAWTC 2 cut(s) 445, 577
Tru1I TTAA 3 cut(s) 329, 350, 772
Tru9I TTAA 3 cut(s) 329, 350, 772
TseI GCWGC 5 cut(s) 92, 118, 133, 229, 622
TspDTI ATGAA 8 cut(s) 71, 206, 225, 353, 534, 555, 692, 696
TspGWI ACGGA 2 cut(s) 302, 684
VpaK11BI GGWCC 4 cut(s) 65, 71, 710, 743
XagI CCTNNNNNAGG 1 cut(s) 751
XapI RAATTY 2 cut(s) 213, 521
XspI CTAG 3 cut(s) 270, 299, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.