Rw4G029550

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014725
Physical Location & Seq
Forward (+)
56218550 .. 56220993
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029550.1

Sequence Viewer

Length: 759 bp
ATGGCAGGGAGAAATCGTATGCCTCGCCATGCTGATGGCTTTCGAGGTTATCGTGATGATCCTCGGCCTGTGATGCACCGTGGACCACAACCGTTTTCTATTCACCCTGCAGCTTTGGAAGAAGAACTTGCAATGCAGCACAGAGAAATGCAACGAATAATCGCTGAGAATCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGGAATTAACAGATGCAAAAGATGAGATTCATAGATTAGGGCAGGTCATACCCAAACTCCGGGCTGAGAAAGAGGCAGAGACTAGGGAGTTGATTGAGAGAGGATTAAAACTTGAAGCTGATCTCCGTGCTGTTGAGCCTGTAAGGGCAGAGGTTTTGCAATTGAGAGCTGAAATTCAGAAATTAAATGCTTTACGGCAGGAGTTGTCTACCCAAGTCCAAGGTCTCACTCAAGACGTCACTCGATTACAAGCTGAGAATCAGCAACTAGTTGCCATGAGGGCTGATATTGATGGCATGCAAAAAGAGCTTGTTGAGACAAGGAGAGCTTATGAATATGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTGATTTCCATGGCTCGTGAAATAGAGCAGCTACGAGCAGAGCAGATGAATGCAGGCAGGAGGGTACGGGGGCTAGCTCCTGAAATGAGATATTCCGGTGGTTCGTATGGCAATGTCTATAGAAATGGTTGGGGACCTTATGACAGAATTCCTAGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

29.3

Weight (kDa)

7.01

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 413
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 2 cut(s) 378, 744
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 3 cut(s) 203, 320, 572
AhlI ACTAGT 1 cut(s) 472
AloI GAACNNNNNNTCC 2 cut(s) 587, 619
AluBI AGCT 8 cut(s) 113, 323, 374, 458, 514, 533, 628, 674
AluI AGCT 8 cut(s) 113, 323, 374, 458, 514, 533, 628, 674
Alw26I GTCTC 3 cut(s) 278, 434, 515
AlwI GGATC 1 cut(s) 53
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 110, 136, 625
ApoI RAATTY 2 cut(s) 378, 744
AspS9I GGNCC 2 cut(s) 83, 731
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 95
AsuNHI GCTAGC 1 cut(s) 670
AvaII GGWCC 2 cut(s) 83, 731
BauI CACGAG 1 cut(s) 612
BbvI GCAGC 3 cut(s) 122, 148, 637
BccI CCATC 2 cut(s) 29, 491
BceAI ACGGC 1 cut(s) 416
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 3 cut(s) 278, 434, 515
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 4 cut(s) 288, 473, 671, 750
BfmI CTRYAG 2 cut(s) 108, 715
BfuAI ACCTGC 1 cut(s) 238
BglI GCCNNNNNGGC 1 cut(s) 485
BisI GCNGC 3 cut(s) 111, 137, 626
BlsI GCNGC 3 cut(s) 112, 138, 627
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 2 cut(s) 83, 731
BmgT120I GGNCC 2 cut(s) 83, 731
BmiI GGNNCC 1 cut(s) 732
BmrFI CCNGG 1 cut(s) 266
BmsI GCATC 2 cut(s) 63, 208
BmtI GCTAGC 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 4 cut(s) 62, 79, 424, 606
BsaWI WCCGGW 1 cut(s) 692
BsaXI ACNNNNNCTCC 3 cut(s) 31, 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse3DI GCAATG 3 cut(s) 138, 591, 715
BseDI CCNNGG 4 cut(s) 62, 79, 424, 606
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 3 cut(s) 138, 591, 715
BseMII CTCAG 3 cut(s) 156, 261, 450
BseXI GCAGC 3 cut(s) 122, 148, 637
BshFI GGCC 1 cut(s) 67
BsiSI CCGG 2 cut(s) 265, 693
BslFI GGGAC 1 cut(s) 744
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 3 cut(s) 278, 434, 515
BsmFI GGGAC 1 cut(s) 744
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 434
Bsp143I GATC 2 cut(s) 58, 325
Bsp19I CCATGG 1 cut(s) 606
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 3 cut(s) 157, 262, 451
BspLI GGNNCC 1 cut(s) 732
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 238
BspOI GCTAGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 3 cut(s) 138, 591, 715
BssECI CCNNGG 4 cut(s) 62, 79, 424, 606
BssMI GATC 2 cut(s) 58, 325
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 2 cut(s) 424, 606
Bst2BI CACGAG 1 cut(s) 612
Bst4CI ACNGT 2 cut(s) 80, 93
BstACI GRCGYC 1 cut(s) 441
BstC8I GCNNGC 4 cut(s) 503, 582, 652, 672
BstDEI CTNAG 3 cut(s) 165, 270, 459
BstDSI CCRYGG 2 cut(s) 79, 606
BstKTI GATC 2 cut(s) 61, 328
BstMAI GTCTC 3 cut(s) 278, 434, 515
BstMBI GATC 2 cut(s) 58, 325
BstMWI GCNNNNNNNGC 3 cut(s) 73, 485, 511
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 1 cut(s) 264
BstSFI CTRYAG 2 cut(s) 108, 715
BstV1I GCAGC 3 cut(s) 122, 148, 637
BstXI CCANNNNNNTGG 1 cut(s) 35
BsuRI GGCC 1 cut(s) 67
BtgI CCRYGG 2 cut(s) 79, 606
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 4 cut(s) 503, 582, 652, 672
Cfr13I GGNCC 2 cut(s) 83, 731
Csp6I GTAC 1 cut(s) 662
CviAII CATG 4 cut(s) 29, 481, 502, 607
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 3 cut(s) 165, 270, 459
DpnI GATC 2 cut(s) 60, 327
DpnII GATC 2 cut(s) 58, 325
Eco130I CCWWGG 2 cut(s) 424, 606
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 83, 731
EcoO109I RGGNCCY 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 744
EcoT14I CCWWGG 2 cut(s) 424, 606
ErhI CCWWGG 2 cut(s) 424, 606
FaeI CATG 4 cut(s) 32, 484, 505, 610
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 517, 549
FaqI GGGAC 1 cut(s) 744
FatI CATG 4 cut(s) 28, 480, 501, 606
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 3 cut(s) 111, 137, 626
Fsp4HI GCNGC 3 cut(s) 111, 137, 626
FspBI CTAG 4 cut(s) 288, 473, 671, 750
GluI GCNGC 3 cut(s) 111, 137, 626
HaeIII GGCC 1 cut(s) 67
HapII CCGG 2 cut(s) 265, 693
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 4 cut(s) 32, 484, 505, 610
HinfI GANTC 3 cut(s) 169, 232, 463
HpaII CCGG 2 cut(s) 265, 693
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 83, 414
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 4 cut(s) 53, 437, 614, 677
Hpy8I GTNNAC 2 cut(s) 83, 414
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 80, 93
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 9 cut(s) 76, 110, 131, 136, 151, 221, 364, 505, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 485, 511
HpyF3I CTNAG 3 cut(s) 165, 270, 459
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 4 cut(s) 32, 484, 505, 610
Kzo9I GATC 2 cut(s) 58, 325
LmnI GCTCC 1 cut(s) 679
Lsp1109I GCAGC 3 cut(s) 122, 148, 637
LweI GCATC 2 cut(s) 63, 208
MaeI CTAG 4 cut(s) 288, 473, 671, 750
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 442
MalI GATC 2 cut(s) 60, 327
MboI GATC 2 cut(s) 58, 325
MboII GAAGA 4 cut(s) 131, 134, 191, 572
MfeI CAATTG 1 cut(s) 365
MluCI AATT 6 cut(s) 180, 209, 365, 378, 386, 744
MnlI CCTC 9 cut(s) 33, 38, 72, 271, 299, 349, 477, 540, 651
MseI TTAA 3 cut(s) 212, 311, 389
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 2 cut(s) 265, 693
MspR9I CCNGG 1 cut(s) 266
MunI CAATTG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 652
MwoI GCNNNNNNNGC 3 cut(s) 73, 485, 511
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 606
NdeII GATC 2 cut(s) 58, 325
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 4 cut(s) 32, 484, 505, 610
NlaIV GGNNCC 1 cut(s) 732
NmeAIII GCCGAG 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 505
PaeI GCATGC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 3 cut(s) 169, 232, 463
PkrI GCNGC 3 cut(s) 112, 138, 627
PpuMI RGGWCCY 1 cut(s) 731
Psp5II RGGWCCY 1 cut(s) 731
PspN4I GGNNCC 1 cut(s) 732
PspPI GGNCC 2 cut(s) 83, 731
PspPPI RGGWCCY 1 cut(s) 731
PstI CTGCAG 1 cut(s) 112
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 3 cut(s) 212, 311, 389
SatI GCNGC 3 cut(s) 111, 137, 626
Sau3AI GATC 2 cut(s) 58, 325
Sau96I GGNCC 2 cut(s) 83, 731
ScrFI CCNGG 1 cut(s) 266
SfaNI GCATC 2 cut(s) 63, 208
SfcI CTRYAG 2 cut(s) 108, 715
SinI GGWCC 2 cut(s) 83, 731
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
SpeI ACTAGT 1 cut(s) 472
SphI GCATGC 1 cut(s) 505
Sse9I AATT 6 cut(s) 180, 209, 365, 378, 386, 744
SspMI CTAG 4 cut(s) 288, 473, 671, 750
StyD4I CCNGG 1 cut(s) 264
StyI CCWWGG 2 cut(s) 424, 606
TaaI ACNGT 2 cut(s) 80, 93
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 43, 448
TasI AATT 6 cut(s) 180, 209, 365, 378, 386, 744
TfiI GAWTC 3 cut(s) 169, 232, 463
Tru1I TTAA 3 cut(s) 212, 311, 389
Tru9I TTAA 3 cut(s) 212, 311, 389
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 110, 136, 625
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 4 cut(s) 224, 552, 573, 659
TspGWI ACGGA 1 cut(s) 320
VpaK11BI GGWCC 2 cut(s) 83, 731
XapI RAATTY 2 cut(s) 378, 744
XceI RCATGY 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 4 cut(s) 288, 473, 671, 750
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.