RLG00000006609

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014725
Physical Location & Seq
Reverse (-)
8982382 .. 8986530
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006609

Sequence Viewer

Length: 1200 bp
ATGGATTTTGAGTGTGAAGGTATCACTAGAGTCGGCCAGCCAAGTTGCGATTTCATCGGAGTGTCGAGATCGAAGCTTTTTCAGGCAGTCACTCCTTCTCTGATCACTGTTATGGCAGGGAGAAATCGTATGCCTCGCCATGCTGATGGCTTTCGAGCTTATCGTGATGATCCTCGGCCTGTTATGCATCGTGGACCACAACCGTTTTCTATTCACCCTGCAGCTTTGGAAGAAGAACTTGCAATGCAGCACAGAGAAATGCAACGAATAATTGCTGAGAATCGGCTTGTAATTGATGATAATACCCTTCTTCAAAGGGAATTAACAGATGCAAAAGATGAGATTCATAGATTAGGGCAGGTCATACCCAAACTCCGGGCCGAGAAAGAGGCAGAGACTAGGGAGTTGATTGAGAGAGGATTAAAACTTGAAGCTGATCTTCGTGCTGTTGAGCCTGTGAGGGCAGAGGTTTTGCAATTGAGAGCTGAAATTCAGAAATTAAATGCTTTACGGCAGGAGTTGTCTACCCAAGTCCAAGGTCTCACTCAAGACGTCACTCAATTACAAGCTGAGAATCAGAAACTAGTTGCCATGAGGACTGATATTGATGGCATGCGAAAAGAGCTTGTTGAGACGAGGAGAGCTTATGAATATGAGAGGAAAGCCAATGAAGAACAAGTTGAACAAAAGCAAGCAATGGAAAAGAACCTGATTTCCATGGCTCGTGAAATAGAGCAGCTACGAGCAGAGCAGATGAATGCAGGCAGGAGGGCACGGGGGCTAGGTGGCGGGGGTTATGGAATGATGAGTGGAGGTCCTGAAATGAGATATTCCGGTGGTTCGTATGGCAATGAGTCTACAGAGACTGACTATATCAATATTAAGCGTTTGGTGGATGACCTATGGGATCGCAGAAAAGTCCACTTGAGAAACAGCCTGCTGCAACAAACCGCTACTTCACCCGCTGCTGCAACTCCCACATCATGTCGAAAGAAGAAGAATGAACAAGCCATTTTTCTGGAATATGTGAAGGATCACATTGATGAATTTATCAATGCATCTGTGGATGAACACGCGTCTTGCTTCAAGAAGACCATCAACAAGATGTTTAAAATGTCAAAGATTGTTACTGAGAAGAATGCTGCTGATACCAAGGAAGTCGAAAGTTCGCTGCCCCTTCACACAACAGTAGCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

45.43

Weight (kDa)

6.55

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 349
AccI GTMKAC 2 cut(s) 524, 857
AccII CGCG 1 cut(s) 1076
AciI CCGC 3 cut(s) 789, 951, 963
AclWI GGATC 3 cut(s) 164, 915, 1041
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 489, 1046
AcyI GRCGYC 1 cut(s) 552
AfiI CCNNNNNNNGG 1 cut(s) 375
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 4 cut(s) 314, 431, 683, 1087
AhlI ACTAGT 1 cut(s) 583
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 9 cut(s) 76, 158, 224, 434, 485, 569, 625, 644, 739
AluI AGCT 9 cut(s) 76, 158, 224, 434, 485, 569, 625, 644, 739
Alw26I GTCTC 4 cut(s) 389, 545, 626, 857
AlwI GGATC 3 cut(s) 164, 915, 1041
AlwNI CAGNNNCTG 1 cut(s) 866
AoxI GGCC 3 cut(s) 34, 176, 378
ApeKI GCWGC 8 cut(s) 221, 247, 736, 940, 965, 968, 1142, 1171
ApoI RAATTY 2 cut(s) 489, 1046
AspS9I GGNCC 3 cut(s) 194, 378, 815
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 2 cut(s) 206, 951
AvaII GGWCC 2 cut(s) 194, 815
BaeGI GKGCMC 1 cut(s) 775
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 1097
BbvI GCAGC 8 cut(s) 233, 259, 748, 927, 952, 955, 1129, 1158
BccI CCATC 3 cut(s) 140, 602, 1103
BceAI ACGGC 1 cut(s) 527
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 4 cut(s) 389, 545, 626, 857
BcuI ACTAGT 1 cut(s) 583
BfaI CTAG 4 cut(s) 27, 399, 584, 782
BfmI CTRYAG 2 cut(s) 219, 858
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 8 cut(s) 222, 248, 737, 941, 966, 969, 1143, 1172
BlsI GCNGC 8 cut(s) 223, 249, 738, 942, 967, 970, 1144, 1173
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 2 cut(s) 194, 815
BmgT120I GGNCC 3 cut(s) 194, 378, 815
BmrFI CCNGG 1 cut(s) 377
BmsI GCATC 3 cut(s) 196, 319, 1067
BpiI GAAGAC 1 cut(s) 1097
BpuEI CTTGAG 2 cut(s) 531, 946
BpuMI CCSGG 1 cut(s) 377
BsaHI GRCGYC 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 4 cut(s) 173, 535, 717, 1152
BsaWI WCCGGW 1 cut(s) 833
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 357, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse3DI GCAATG 3 cut(s) 249, 702, 856
BseDI CCNNGG 4 cut(s) 173, 535, 717, 1152
BseGI GGATG 2 cut(s) 901, 1072
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMI GCAATG 3 cut(s) 249, 702, 856
BseMII CTCAG 3 cut(s) 267, 561, 1122
BseRI GAGGAG 1 cut(s) 652
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 8 cut(s) 233, 259, 748, 927, 952, 955, 1129, 1158
Bsh1236I CGCG 1 cut(s) 1076
BshFI GGCC 3 cut(s) 36, 178, 380
BsiSI CCGG 2 cut(s) 376, 834
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 4 cut(s) 389, 545, 626, 857
BsmBI CGTCTC 1 cut(s) 626
BsmI GAATGC 2 cut(s) 763, 1144
BsnI GGCC 3 cut(s) 36, 178, 380
Bso31I GGTCTC 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
Bsp19I CCATGG 1 cut(s) 717
BspACI CCGC 3 cut(s) 789, 951, 963
BspANI GGCC 3 cut(s) 36, 178, 380
BspCNI CTCAG 3 cut(s) 268, 562, 1123
BspFNI CGCG 1 cut(s) 1076
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 3 cut(s) 164, 915, 1041
BspTNI GGTCTC 1 cut(s) 545
BsrDI GCAATG 3 cut(s) 249, 702, 856
BssECI CCNNGG 4 cut(s) 173, 535, 717, 1152
BssMI GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
BssNI GRCGYC 1 cut(s) 552
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 3 cut(s) 535, 717, 1152
Bst2BI CACGAG 1 cut(s) 723
Bst4CI ACNGT 3 cut(s) 109, 204, 1189
BstACI GRCGYC 1 cut(s) 552
BstC8I GCNNGC 5 cut(s) 38, 614, 693, 763, 938
BstDEI CTNAG 3 cut(s) 276, 570, 1131
BstDSI CCRYGG 1 cut(s) 717
BstF5I GGATG 2 cut(s) 901, 1072
BstFNI CGCG 1 cut(s) 1076
BstKTI GATC 6 cut(s) 71, 105, 172, 439, 910, 1036
BstMAI GTCTC 4 cut(s) 389, 545, 626, 857
BstMBI GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 184, 622
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 375
BstSFI CTRYAG 2 cut(s) 219, 858
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 1 cut(s) 1076
BstV1I GCAGC 8 cut(s) 233, 259, 748, 927, 952, 955, 1129, 1158
BstV2I GAAGAC 1 cut(s) 1097
BstXI CCANNNNNNTGG 2 cut(s) 146, 1018
BsuRI GGCC 3 cut(s) 36, 178, 380
BtgI CCRYGG 1 cut(s) 717
BtsCI GGATG 2 cut(s) 901, 1072
BtsIMutI CAGTG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 5 cut(s) 38, 614, 693, 763, 938
CaiI CAGNNNCTG 1 cut(s) 866
Cfr13I GGNCC 3 cut(s) 194, 378, 815
CseI GACGC 1 cut(s) 1065
CviAII CATG 5 cut(s) 140, 592, 613, 718, 984
DdeI CTNAG 3 cut(s) 276, 570, 1131
DpnI GATC 6 cut(s) 70, 104, 171, 438, 909, 1035
DpnII GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
DraI TTTAAA 1 cut(s) 1111
EaeI YGGCCR 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 535, 717, 1152
Eco31I GGTCTC 1 cut(s) 545
Eco47I GGWCC 2 cut(s) 194, 815
EcoO109I RGGNCCY 1 cut(s) 815
EcoT14I CCWWGG 3 cut(s) 535, 717, 1152
EcoT22I ATGCAT 2 cut(s) 189, 1060
ErhI CCWWGG 3 cut(s) 535, 717, 1152
Esp3I CGTCTC 1 cut(s) 626
FaeI CATG 5 cut(s) 143, 595, 616, 721, 987
FalI AAGNNNNNCTT 4 cut(s) 222, 254, 423, 455
FatI CATG 5 cut(s) 139, 591, 612, 717, 983
FauI CCCGC 2 cut(s) 782, 970
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 2 cut(s) 524, 857
Fnu4HI GCNGC 8 cut(s) 222, 248, 737, 941, 966, 969, 1143, 1172
FokI GGATG 2 cut(s) 908, 1079
Fsp4HI GCNGC 8 cut(s) 222, 248, 737, 941, 966, 969, 1143, 1172
FspBI CTAG 4 cut(s) 27, 399, 584, 782
GluI GCNGC 8 cut(s) 222, 248, 737, 941, 966, 969, 1143, 1172
HaeIII GGCC 3 cut(s) 36, 178, 380
HapII CCGG 2 cut(s) 376, 834
HgaI GACGC 1 cut(s) 1065
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 5 cut(s) 143, 595, 616, 721, 987
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 5 cut(s) 30, 280, 343, 574, 854
HpaII CCGG 2 cut(s) 376, 834
HphI GGTGA 2 cut(s) 206, 951
Hpy166II GTNNAC 4 cut(s) 194, 525, 858, 922
Hpy188I TCNGA 4 cut(s) 59, 102, 495, 579
Hpy188III TCNNGA 7 cut(s) 66, 164, 548, 725, 818, 1019, 1087
Hpy8I GTNNAC 4 cut(s) 194, 525, 858, 922
HpyAV CCTTC 5 cut(s) 11, 105, 317, 1024, 1187
HpyCH4III ACNGT 3 cut(s) 109, 204, 1189
HpyCH4IV ACGT 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 622
HpyF3I CTNAG 3 cut(s) 276, 570, 1131
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 5 cut(s) 143, 595, 616, 721, 987
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
Lsp1109I GCAGC 8 cut(s) 233, 259, 748, 927, 952, 955, 1129, 1158
LweI GCATC 3 cut(s) 196, 319, 1067
MaeI CTAG 4 cut(s) 27, 399, 584, 782
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 3 cut(s) 88, 553, 1126
MalI GATC 6 cut(s) 70, 104, 171, 438, 909, 1035
MboI GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
MboII GAAGA 9 cut(s) 242, 245, 302, 431, 683, 1006, 1009, 1102, 1147
MfeI CAATTG 1 cut(s) 476
MhlI GDGCHC 1 cut(s) 775
MluCI AATT 8 cut(s) 270, 291, 320, 476, 489, 497, 560, 1046
MluI ACGCGT 1 cut(s) 1074
MlyI GAGTC 2 cut(s) 39, 863
Mph1103I ATGCAT 2 cut(s) 189, 1060
MseI TTAA 5 cut(s) 323, 422, 500, 882, 1110
MslI CAYNNNNRTG 4 cut(s) 59, 110, 144, 1041
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 2 cut(s) 376, 834
MspR9I CCNGG 1 cut(s) 377
MunI CAATTG 1 cut(s) 476
Mva1269I GAATGC 2 cut(s) 763, 1144
MvnI CGCG 1 cut(s) 1076
MwoI GCNNNNNNNGC 2 cut(s) 184, 622
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 717
NdeII GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
NlaIII CATG 5 cut(s) 143, 595, 616, 721, 987
NmeAIII GCCGAG 2 cut(s) 154, 406
NmuCI GTSAC 2 cut(s) 88, 553
NsiI ATGCAT 2 cut(s) 189, 1060
NspI RCATGY 1 cut(s) 616
PaeI GCATGC 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PctI GAATGC 2 cut(s) 763, 1144
PfeI GAWTC 3 cut(s) 280, 343, 574
PkrI GCNGC 8 cut(s) 223, 249, 738, 942, 967, 970, 1144, 1173
PleI GAGTC 2 cut(s) 38, 862
PpsI GAGTC 2 cut(s) 38, 862
PpuMI RGGWCCY 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 815
PspPI GGNCC 3 cut(s) 194, 378, 815
PspPPI RGGWCCY 1 cut(s) 815
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 866
RseI CAYNNNNRTG 4 cut(s) 59, 110, 144, 1041
SaqAI TTAA 5 cut(s) 323, 422, 500, 882, 1110
SatI GCNGC 8 cut(s) 222, 248, 737, 941, 966, 969, 1143, 1172
Sau3AI GATC 6 cut(s) 68, 102, 169, 436, 907, 1033
Sau96I GGNCC 3 cut(s) 194, 378, 815
SchI GAGTC 2 cut(s) 39, 863
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 3 cut(s) 196, 319, 1067
SfcI CTRYAG 2 cut(s) 219, 858
SinI GGWCC 2 cut(s) 194, 815
SmiMI CAYNNNNRTG 4 cut(s) 59, 110, 144, 1041
SmlI CTYRAG 2 cut(s) 546, 925
SmoI CTYRAG 2 cut(s) 546, 925
SpeI ACTAGT 1 cut(s) 583
SphI GCATGC 1 cut(s) 616
Sse9I AATT 8 cut(s) 270, 291, 320, 476, 489, 497, 560, 1046
SsiI CCGC 3 cut(s) 789, 951, 963
SspI AATATT 1 cut(s) 880
SspMI CTAG 4 cut(s) 27, 399, 584, 782
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 3 cut(s) 535, 717, 1152
TaaI ACNGT 3 cut(s) 109, 204, 1189
TaiI ACGT 1 cut(s) 555
TaqI TCGA 5 cut(s) 65, 71, 154, 988, 1161
TasI AATT 8 cut(s) 270, 291, 320, 476, 489, 497, 560, 1046
TfiI GAWTC 3 cut(s) 280, 343, 574
Tru1I TTAA 5 cut(s) 323, 422, 500, 882, 1110
Tru9I TTAA 5 cut(s) 323, 422, 500, 882, 1110
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 2 cut(s) 88, 553
TseI GCWGC 8 cut(s) 221, 247, 736, 940, 965, 968, 1142, 1171
Tsp45I GTSAC 2 cut(s) 88, 553
TspDTI ATGAA 8 cut(s) 43, 335, 663, 684, 770, 1017, 1059, 1083
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 194, 815
XapI RAATTY 2 cut(s) 489, 1046
XceI RCATGY 1 cut(s) 616
XmiI GTMKAC 2 cut(s) 524, 857
XspI CTAG 4 cut(s) 27, 399, 584, 782
ZraI GACGTC 1 cut(s) 553
Zsp2I ATGCAT 2 cut(s) 189, 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.