Prupe.1G310800_v2.0.a1

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014725
Physical Location & Seq
Reverse (-)
30213885 .. 30216651
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G310800.1

Sequence Viewer

Length: 792 bp
ATGTCCGGGAGAAATCGTATGTCTCGCCATGCTGATGGTTATCGAGGTTACCATGATGATCCTCGACCTCTCATGAACCGAGGACTGGGACACTTGAGTGTACACCCTGCAGCTTTGGAAGAAGAATTTGCAATGAAACACAGAGAAATGCAGCGAATCGTTGCTGAGAACCGGCTTGTAATTGACGATAATACCCTTCTTCAAAGGGAATTGACAGATGCGAAAGATGAGATTCATAGATTGGGGCAGGTCTTACCTAAACTCCGGGCTGACAAAGAGGCACAGTCTAGGGAGCTAATTGAGAAAGGATTGAAGCTAGAAGCTGATCTCCGTGCTACTGAGCCTATAAAGGCAGAGGTTATGCAGTTAAGAGCTGAAGTGCAGAAATTAAGTGCTTTGCGGCAAGAGTTGTCTTCCCAAGTCCAAGGTCTGACACAGGAGATAACTCGATTGCAAGCTGAGAATCAGCAGCTAATTCCCATGAGGGCTGATATTGATGGGATGCGAAACGAGCTTGTTGAGACCAGGAGAGCTTATGAATATGAAAGGAAAGCCAGTGAAGAACAAGTGGAACAAAAGCAAGCAATGGAAAATAACCTTGTTTCCATGGCTCGTGAAATAGAGAAGCTAAGAGCAGAGCAGCTAAATGCAGACAGGAGGGCACGAGAACGAGGTGGTGGAGGTTATGGAATGATGAATGGAAGTCCTGGTATGAGATATTCACGTGGTTCATATGGCAATGGCTACAGCAGTGGTTGGGGACCTTATGATAAGCGTGGTTCACGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

30.17

Weight (kDa)

9.02

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AciI CCGC 1 cut(s) 400
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 396
AcvI CACGTG 1 cut(s) 725
AfaI GTAC 1 cut(s) 102
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 2 cut(s) 203, 313
AjnI CCWGG 2 cut(s) 524, 706
AleI CACNNNNGTG 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 27, 515
AlwI GGATC 1 cut(s) 53
ApeKI GCWGC 4 cut(s) 110, 151, 469, 640
ApoI RAATTY 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 761
AsuC2I CCSGG 2 cut(s) 7, 266
AvaII GGWCC 1 cut(s) 761
BaeGI GKGCMC 1 cut(s) 664
BauI CACGAG 2 cut(s) 612, 663
BbrPI CACGTG 1 cut(s) 725
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 4 cut(s) 122, 163, 481, 652
BccI CCATC 2 cut(s) 29, 491
BciT130I CCWGG 2 cut(s) 526, 708
BcnI CCSGG 2 cut(s) 7, 266
BcoDI GTCTC 2 cut(s) 27, 515
BfaI CTAG 2 cut(s) 288, 317
BfmI CTRYAG 2 cut(s) 108, 745
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 5 cut(s) 111, 152, 401, 470, 641
BlsI GCNGC 5 cut(s) 112, 153, 402, 471, 642
Bme1390I CCNGG 4 cut(s) 7, 266, 526, 708
Bme18I GGWCC 1 cut(s) 761
BmgT120I GGNCC 1 cut(s) 761
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 4 cut(s) 7, 266, 526, 708
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 2 cut(s) 208, 492
BmuI ACTGGG 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 115
BpuMI CCSGG 2 cut(s) 7, 266
BsaAI YACGTR 1 cut(s) 725
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 3 cut(s) 79, 424, 606
BsaXI ACNNNNNCTCC 3 cut(s) 31, 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 2 cut(s) 90, 555
Bse3DI GCAATG 3 cut(s) 138, 591, 745
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 2 cut(s) 526, 708
BseDI CCNNGG 3 cut(s) 79, 424, 606
BseGI GGATG 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 3 cut(s) 138, 591, 745
BseMII CTCAG 3 cut(s) 156, 330, 450
BseNI ACTGG 2 cut(s) 90, 555
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 4 cut(s) 122, 163, 481, 652
BsgI GTGCAG 1 cut(s) 401
BsiSI CCGG 3 cut(s) 6, 172, 265
BslFI GGGAC 2 cut(s) 102, 774
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 2 cut(s) 27, 515
BsmFI GGGAC 2 cut(s) 102, 774
Bso31I GGTCTC 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 664
Bsp1407I TGTACA 1 cut(s) 100
Bsp143I GATC 2 cut(s) 58, 325
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 1 cut(s) 400
BspCNI CTCAG 3 cut(s) 157, 331, 451
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 515
BsrDI GCAATG 3 cut(s) 138, 591, 745
BsrFI RCCGGY 1 cut(s) 171
BsrGI TGTACA 1 cut(s) 100
BsrI ACTGG 2 cut(s) 90, 555
BssAI RCCGGY 1 cut(s) 171
BssECI CCNNGG 3 cut(s) 79, 424, 606
BssMI GATC 2 cut(s) 58, 325
BssSI CACGAG 2 cut(s) 612, 663
BssT1I CCWWGG 2 cut(s) 424, 606
Bst2BI CACGAG 2 cut(s) 612, 663
Bst2UI CCWGG 2 cut(s) 526, 708
Bst4CI ACNGT 1 cut(s) 285
BstAUI TGTACA 1 cut(s) 100
BstBAI YACGTR 1 cut(s) 725
BstC8I GCNNGC 2 cut(s) 456, 582
BstDEI CTNAG 4 cut(s) 165, 339, 459, 629
BstDSI CCRYGG 1 cut(s) 606
BstEII GGTNACC 1 cut(s) 47
BstF5I GGATG 1 cut(s) 507
BstKTI GATC 2 cut(s) 61, 328
BstMAI GTCTC 2 cut(s) 27, 515
BstMBI GATC 2 cut(s) 58, 325
BstMWI GCNNNNNNNGC 1 cut(s) 511
BstNI CCWGG 2 cut(s) 526, 708
BstPI GGTNACC 1 cut(s) 47
BstSCI CCNGG 4 cut(s) 5, 264, 524, 706
BstSFI CTRYAG 2 cut(s) 108, 745
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 4 cut(s) 122, 163, 481, 652
BstV2I GAAGAC 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 2 cut(s) 562, 757
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 2 cut(s) 456, 582
CciI TCATGA 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 761
Csp6I GTAC 1 cut(s) 101
CviAII CATG 5 cut(s) 29, 53, 73, 481, 607
CviQI GTAC 1 cut(s) 101
DdeI CTNAG 4 cut(s) 165, 339, 459, 629
DpnI GATC 2 cut(s) 60, 327
DpnII GATC 2 cut(s) 58, 325
Eco130I CCWWGG 2 cut(s) 424, 606
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 1 cut(s) 761
Eco57I CTGAAG 1 cut(s) 396
Eco72I CACGTG 1 cut(s) 725
Eco91I GGTNACC 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 761
EcoO65I GGTNACC 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 524, 706
EcoT14I CCWWGG 2 cut(s) 424, 606
ErhI CCWWGG 2 cut(s) 424, 606
FaeI CATG 5 cut(s) 32, 56, 76, 484, 610
FaqI GGGAC 2 cut(s) 102, 774
FatI CATG 5 cut(s) 28, 52, 72, 480, 606
FauNDI CATATG 1 cut(s) 733
Fnu4HI GCNGC 5 cut(s) 111, 152, 401, 470, 641
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 5 cut(s) 111, 152, 401, 470, 641
FspBI CTAG 2 cut(s) 288, 317
GluI GCNGC 5 cut(s) 111, 152, 401, 470, 641
HapII CCGG 3 cut(s) 6, 172, 265
Hin1II CATG 5 cut(s) 32, 56, 76, 484, 610
HinfI GANTC 3 cut(s) 156, 232, 463
HpaII CCGG 3 cut(s) 6, 172, 265
Hpy166II GTNNAC 3 cut(s) 101, 103, 782
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 2 cut(s) 73, 614
Hpy8I GTNNAC 3 cut(s) 101, 103, 782
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 285
HpyCH4IV ACGT 1 cut(s) 724
HpyCH4V TGCA 7 cut(s) 110, 131, 151, 364, 382, 454, 650
HpyF10VI GCNNNNNNNGC 1 cut(s) 511
HpyF3I CTNAG 4 cut(s) 165, 339, 459, 629
HpySE526I ACGT 1 cut(s) 724
Hsp92II CATG 5 cut(s) 32, 56, 76, 484, 610
Kzo9I GATC 2 cut(s) 58, 325
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 4 cut(s) 122, 163, 481, 652
LweI GCATC 2 cut(s) 208, 492
MaeI CTAG 2 cut(s) 288, 317
MaeII ACGT 1 cut(s) 724
MaeIII GTNAC 1 cut(s) 47
MalI GATC 2 cut(s) 60, 327
MboI GATC 2 cut(s) 58, 325
MboII GAAGA 5 cut(s) 131, 134, 191, 405, 572
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 6 cut(s) 125, 180, 209, 297, 386, 474
MseI TTAA 3 cut(s) 368, 389, 790
MslI CAYNNNNRTG 2 cut(s) 33, 96
MspI CCGG 3 cut(s) 6, 172, 265
MspR9I CCNGG 4 cut(s) 7, 266, 526, 708
MvaI CCWGG 2 cut(s) 526, 708
MwoI GCNNNNNNNGC 1 cut(s) 511
NciI CCSGG 2 cut(s) 7, 266
NcoI CCATGG 1 cut(s) 606
NdeI CATATG 1 cut(s) 733
NdeII GATC 2 cut(s) 58, 325
NlaIII CATG 5 cut(s) 32, 56, 76, 484, 610
NlaIV GGNNCC 1 cut(s) 762
OliI CACNNNNGTG 1 cut(s) 96
PagI TCATGA 1 cut(s) 72
PfeI GAWTC 3 cut(s) 156, 232, 463
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 5 cut(s) 112, 153, 402, 471, 642
PmaCI CACGTG 1 cut(s) 725
PmlI CACGTG 1 cut(s) 725
Ppu21I YACGTR 1 cut(s) 725
PpuMI RGGWCCY 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 2 cut(s) 524, 706
PspCI CACGTG 1 cut(s) 725
PspEI GGTNACC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 524, 706
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 1 cut(s) 761
PspPPI RGGWCCY 1 cut(s) 761
PstI CTGCAG 1 cut(s) 112
RsaI GTAC 1 cut(s) 102
RsaNI GTAC 1 cut(s) 101
RseI CAYNNNNRTG 2 cut(s) 33, 96
SaqAI TTAA 3 cut(s) 368, 389, 790
SatI GCNGC 5 cut(s) 111, 152, 401, 470, 641
Sau3AI GATC 2 cut(s) 58, 325
Sau96I GGNCC 1 cut(s) 761
ScrFI CCNGG 4 cut(s) 7, 266, 526, 708
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 2 cut(s) 208, 492
SfcI CTRYAG 2 cut(s) 108, 745
SinI GGWCC 1 cut(s) 761
SmiMI CAYNNNNRTG 2 cut(s) 33, 96
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 6 cut(s) 125, 180, 209, 297, 386, 474
SsiI CCGC 1 cut(s) 400
SspMI CTAG 2 cut(s) 288, 317
StyD4I CCNGG 4 cut(s) 5, 264, 524, 706
StyI CCWWGG 2 cut(s) 424, 606
TaaI ACNGT 1 cut(s) 285
TaiI ACGT 1 cut(s) 727
TaqI TCGA 3 cut(s) 43, 64, 448
TasI AATT 6 cut(s) 125, 180, 209, 297, 386, 474
TatI WGTACW 1 cut(s) 100
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 3 cut(s) 156, 232, 463
Tru1I TTAA 3 cut(s) 368, 389, 790
Tru9I TTAA 3 cut(s) 368, 389, 790
TscAI CASTG 2 cut(s) 562, 757
TseI GCWGC 4 cut(s) 110, 151, 469, 640
TspDTI ATGAA 7 cut(s) 89, 149, 224, 552, 558, 710, 720
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 2 cut(s) 562, 757
VpaK11BI GGWCC 1 cut(s) 761
XapI RAATTY 1 cut(s) 125
XspI CTAG 2 cut(s) 288, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.