FvH4_4g27580

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011790
Physical Location & Seq
Reverse (-)
28526476 .. 28529943
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27580.t1

Sequence Viewer

Length: 1440 bp
ATGGCCTCCTCTCCTTTCCTCTCCAAGTCCCCCATTTCCTTCTCCTCTTCCGTCTCCACCTCCCTCCCATGGCGCCCTTCTCTCTCCTCCTCCTCCTCCGCCGCATTCCGCTCCAAGCCCCGCCGCAGGTCCCTCACCGTCCAATCCAAGATCAGGGAGATCTTCATGCCCGCCCTCAGCTCCACCATGACCGAGGGCAAGATCGTCTCCTGGATCAAATCCGAAGGCGACGTCCTCTCCAAGGGCGAGAGCGTCGTCGTCGTCGAGTCCGATAAAGCCGACATGGACGTCGAGACTTTCTACGACGGCATCCTCGCCGCGATTGTCGTCGGCGAGGGCGAGTCCGCCCCCGTCGGCGCCGCCATCGGGATTCTCGCCGAGACTGAAGCCGAGCTCGAAGAGGCCAAGGCCAAAGCCAAGACTGATTCCGGCGCCGCGAAGCCCGAGGCCGCTGCCGTTACGCCCTCTACTCCTCCTGTTACTGCCAATCCTGCTCCTGCTATTGCGCAGCCTGCTCCTGCTGCCACGTCAGCTGCGCCGGCTGGGCCCAGGAAGACTGTGGCCACTCCCTTTGCGAAGAAGCTGGCTAAGCAGCACAAGGTGGACATTGCCGCCGTGGTGGGGACCGGCCCGTTCGGGAGAATTACTCCTGCTGACGTGGAGGCTGCCGCGGGAATTCAGCCCAAGAAATCCGTGGCTGTAGAGTCTGCTACGGCTGCGGCGGCTCCGTCCAAGCCTGCTGCCGCCCCGGCATTGCCTCTACTTCCAGGCTCGACTGTCGTGCCTTTCACCACAATGCAGGCTGCAGTTTCGAAGAATATGATTGAGAGTCTCTCGGTGCCGACTTTCCGGGTGGGTTATCCGGTGAACAGCGATGCCATCGATGCGCTGTATGCGAAGTTGAAATCAAAGGGTGTGACCATGACTGCCTTGCTTGCCAAGGCGGTTGCTCTGGCGCTCGCTCAGCACCCGGTTGTGAATGCCAGCTGCAAAGACGGAAAGAGCTTTTACTACAACAGTAGCATAAACATCGCCGTTGCTGTGGCTATTGACGGCGGGTTGATAACCCCTGTTCTTCAGGATGCAGATAAGGTGGATTTGTATTTGTTATCCCAGAAATGGAAAGAGCTGGTGGGAAAGGCCCGCTCAAAGCAACTTCAGCCCCATGAGTACAACTCAGGAACTTTCACTCTCTCCAATTTGGGTATGTTTGGAGTGGACAGGTTCGATGCTATTCTTCCTCCAGGCCAGGGTGCTATCATGGCTGTTGGAGCTTCAAAACCCACCGTTGTAGCTGATGCAGACGGATTCTTCAGTGTAAAAAGTAAAATGCTGGTGAATGTAACAGCAGATCACCGAATTGTTTATGGAGCTGATTTGGCTGCCTTCCTAAAGACATTTGCAAAGATTGTTGAGAACCCAGAAAGCCTGACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

480

Amino Acids

49.54

Weight (kDa)

9.05

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 53 - 123 6.7e-14 Biotin-requiring enzyme
E3_binding PF02817 187 - 221 2.3e-13 e3 binding domain
2-oxoacid_dh PF00198 258 - 478 1e-63 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25860
fragaria_vesca FvH4_4g27580
malus_domestica MD13G1043400.v1.1 MD16G1044200.v1.1
prunus_persica Prupe.1G309100_v2.0.a1
pyrus_communis pycom13g03820 pycom16g03840
rosa_chinensis RchiOBHm_Chr4g0435441
rosa_laevigata RLG00000006592
rosa_multiflora Rmu_sc0008521.1_g000001
rosa_roxburghii Rroxscaffold_5G00376680
rosa_rugosa Rorug04G0285000 Rorug04G0285100 Rorug05G0266800
rosa_samantha Rh4AG340800 Rh4BG349100 Rh4CG363600 Rh4DG343300
rosa_wichuraiana Rw4G029720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AatII GACGTC 2 cut(s) 234, 291
Acc16I TGCGCA 1 cut(s) 507
Acc36I ACCTGC 1 cut(s) 117
AccB1I GGYRCC 4 cut(s) 72, 356, 431, 838
AccBSI CCGCTC 2 cut(s) 111, 1146
AccII CGCG 3 cut(s) 320, 437, 671
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 1 cut(s) 675
AcuI CTGAAG 4 cut(s) 405, 1061, 1142, 1297
AcyI GRCGYC 5 cut(s) 73, 231, 288, 357, 432
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 1 cut(s) 1172
AfiI CCNNNNNNNGG 8 cut(s) 69, 126, 153, 241, 366, 621, 1119, 1250
AgsI TTSAA 2 cut(s) 904, 1278
AjiI CACGTC 2 cut(s) 528, 658
AjnI CCWGG 5 cut(s) 209, 548, 766, 1243, 1248
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 5 cut(s) 58, 211, 287, 374, 836
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 9 cut(s) 3, 402, 408, 447, 545, 561, 628, 1140, 1246
ApaI GGGCCC 1 cut(s) 549
ApoI RAATTY 1 cut(s) 675
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
AspLEI GCGC 7 cut(s) 75, 359, 434, 508, 538, 889, 958
AspS9I GGNCC 6 cut(s) 129, 545, 546, 624, 629, 1141
AsuC2I CCSGG 3 cut(s) 749, 851, 971
AsuHPI GGTGA 5 cut(s) 127, 781, 877, 1346, 1348
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 2 cut(s) 129, 624
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 1 cut(s) 563
BanI GGYRCC 4 cut(s) 72, 356, 431, 838
BanII GRGCYC 2 cut(s) 396, 549
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 1 cut(s) 396
BbvCI CCTCAGC 1 cut(s) 176
BccI CCATC 2 cut(s) 371, 887
BceAI ACGGC 6 cut(s) 322, 440, 599, 729, 1019, 1069
BcgI CGANNNNNNTGC 6 cut(s) 434, 468, 763, 797, 1012, 1046
BciT130I CCWGG 5 cut(s) 211, 550, 768, 1245, 1250
BcnI CCSGG 3 cut(s) 749, 851, 971
BcoDI GTCTC 5 cut(s) 58, 211, 287, 374, 836
BfmI CTRYAG 2 cut(s) 699, 804
BfoI RGCGCY 4 cut(s) 76, 360, 435, 959
BfuAI ACCTGC 1 cut(s) 117
BglI GCCNNNNNGGC 2 cut(s) 544, 749
BglII AGATCT 1 cut(s) 159
BlpI GCTNAGC 2 cut(s) 588, 963
Bme1390I CCNGG 8 cut(s) 211, 550, 749, 768, 851, 971, 1245, 1250
Bme18I GGWCC 2 cut(s) 129, 624
BmeT110I CYCGRG 1 cut(s) 443
BmgBI CACGTC 2 cut(s) 528, 658
BmgT120I GGNCC 6 cut(s) 129, 545, 546, 624, 629, 1141
BmiI GGNNCC 8 cut(s) 74, 131, 358, 433, 547, 625, 726, 840
BmrFI CCNGG 8 cut(s) 211, 550, 749, 768, 851, 971, 1245, 1250
BmsI GCATC 6 cut(s) 318, 865, 874, 1072, 1219, 1288
BpiI GAAGAC 1 cut(s) 560
BplI GAGNNNNNCTC 6 cut(s) 631, 663, 818, 850, 1160, 1192
BpmI CTGGAG 1 cut(s) 1227
Bpu10I CCTNAGC 1 cut(s) 176
Bpu1102I GCTNAGC 2 cut(s) 588, 963
Bpu14I TTCGAA 1 cut(s) 812
BpuMI CCSGG 3 cut(s) 749, 851, 971
Bsa29I ATCGAT 1 cut(s) 882
BsaHI GRCGYC 5 cut(s) 73, 231, 288, 357, 432
BsaWI WCCGGW 1 cut(s) 862
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 8 cut(s) 69, 126, 153, 241, 366, 621, 1119, 1250
Bse118I RCCGGY 2 cut(s) 538, 626
Bse3DI GCAATG 2 cut(s) 606, 752
BseBI CCWGG 5 cut(s) 211, 550, 768, 1245, 1250
BseCI ATCGAT 1 cut(s) 882
BseGI GGATG 2 cut(s) 309, 1087
BseLI CCNNNNNNNGG 8 cut(s) 69, 126, 153, 241, 366, 621, 1119, 1250
BseMI GCAATG 2 cut(s) 606, 752
BseMII CTCAG 3 cut(s) 190, 977, 1191
BseRI GAGGAG 6 cut(s) 34, 76, 79, 82, 85, 462
BseSI GKGCMC 1 cut(s) 549
BseYI CCCAGC 1 cut(s) 542
Bsh1236I CGCG 3 cut(s) 320, 437, 671
BshFI GGCC 9 cut(s) 5, 404, 410, 449, 547, 563, 630, 1142, 1248
BshNI GGYRCC 4 cut(s) 72, 356, 431, 838
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 7 cut(s) 429, 539, 627, 749, 850, 863, 971
BslFI GGGAC 3 cut(s) 13, 115, 637
BslI CCNNNNNNNGG 8 cut(s) 69, 126, 153, 241, 366, 621, 1119, 1250
BsmAI GTCTC 5 cut(s) 58, 211, 287, 374, 836
BsmBI CGTCTC 2 cut(s) 58, 211
BsmFI GGGAC 3 cut(s) 13, 115, 637
BsmI GAATGC 2 cut(s) 104, 985
BsnI GGCC 9 cut(s) 5, 404, 410, 449, 547, 563, 630, 1142, 1248
BsoBI CYCGRG 1 cut(s) 443
Bsp119I TTCGAA 1 cut(s) 812
Bsp120I GGGCCC 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 396, 549
Bsp143I GATC 5 cut(s) 150, 159, 201, 213, 1351
Bsp1720I GCTNAGC 2 cut(s) 588, 963
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 9 cut(s) 5, 404, 410, 449, 547, 563, 630, 1142, 1248
BspCNI CTCAG 3 cut(s) 189, 976, 1190
BspDI ATCGAT 1 cut(s) 882
BspFNI CGCG 3 cut(s) 320, 437, 671
BspLI GGNNCC 8 cut(s) 74, 131, 358, 433, 547, 625, 726, 840
BspMAI CTGCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 4 cut(s) 72, 356, 431, 838
BsrBI CCGCTC 2 cut(s) 111, 1146
BsrDI GCAATG 2 cut(s) 606, 752
BsrFI RCCGGY 2 cut(s) 538, 626
BssAI RCCGGY 2 cut(s) 538, 626
BssMI GATC 5 cut(s) 150, 159, 201, 213, 1351
BssNI GRCGYC 5 cut(s) 73, 231, 288, 357, 432
BssT1I CCWWGG 4 cut(s) 68, 240, 405, 939
Bst2UI CCWGG 5 cut(s) 211, 550, 768, 1245, 1250
Bst4CI ACNGT 5 cut(s) 139, 559, 778, 1019, 1288
Bst6I CTCTTC 2 cut(s) 52, 393
BstACI GRCGYC 5 cut(s) 73, 231, 288, 357, 432
BstBI TTCGAA 1 cut(s) 812
BstDEI CTNAG 4 cut(s) 176, 588, 963, 1177
BstDSI CCRYGG 4 cut(s) 68, 615, 669, 693
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 309, 1087
BstFNI CGCG 3 cut(s) 320, 437, 671
BstH2I RGCGCY 4 cut(s) 76, 360, 435, 959
BstHHI GCGC 7 cut(s) 75, 359, 434, 508, 538, 889, 958
BstKTI GATC 5 cut(s) 153, 162, 204, 216, 1354
BstMAI GTCTC 5 cut(s) 58, 211, 287, 374, 836
BstMBI GATC 5 cut(s) 150, 159, 201, 213, 1351
BstNI CCWGG 5 cut(s) 211, 550, 768, 1245, 1250
BstSCI CCNGG 8 cut(s) 209, 548, 747, 766, 849, 969, 1243, 1248
BstSFI CTRYAG 2 cut(s) 699, 804
BstSLI GKGCMC 1 cut(s) 549
BstUI CGCG 3 cut(s) 320, 437, 671
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
Bsu15I ATCGAT 1 cut(s) 882
BsuRI GGCC 9 cut(s) 5, 404, 410, 449, 547, 563, 630, 1142, 1248
BsuTUI ATCGAT 1 cut(s) 882
BtgI CCRYGG 4 cut(s) 68, 615, 669, 693
BtgZI GCGATG 2 cut(s) 888, 1015
BtrI CACGTC 2 cut(s) 528, 658
BtsCI GGATG 2 cut(s) 309, 1087
BtsIMutI CAGTG 1 cut(s) 1321
BveI ACCTGC 1 cut(s) 117
CfoI GCGC 7 cut(s) 75, 359, 434, 508, 538, 889, 958
Cfr10I RCCGGY 2 cut(s) 538, 626
Cfr13I GGNCC 6 cut(s) 129, 545, 546, 624, 629, 1141
Cfr42I CCGCGG 1 cut(s) 672
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 1171
CspCI CAANNNNNGTGG 2 cut(s) 553, 588
CviAII CATG 7 cut(s) 69, 166, 187, 283, 922, 1166, 1261
CviQI GTAC 1 cut(s) 1171
DdeI CTNAG 4 cut(s) 176, 588, 963, 1177
DinI GGCGCC 3 cut(s) 74, 358, 433
DpnI GATC 5 cut(s) 152, 161, 203, 215, 1353
DpnII GATC 5 cut(s) 150, 159, 201, 213, 1351
DraIII CACNNNGTG 1 cut(s) 601
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 2 cut(s) 52, 393
EarI CTCTTC 2 cut(s) 52, 393
EciI GGCGGA 2 cut(s) 88, 334
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 4 cut(s) 68, 240, 405, 939
Eco24I GRGCYC 2 cut(s) 396, 549
Eco47I GGWCC 2 cut(s) 129, 624
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 4 cut(s) 405, 1061, 1142, 1297
Eco88I CYCGRG 1 cut(s) 443
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 675
EcoRII CCWGG 5 cut(s) 209, 548, 766, 1243, 1248
EcoT14I CCWWGG 4 cut(s) 68, 240, 405, 939
EcoT38I GRGCYC 2 cut(s) 396, 549
EgeI GGCGCC 3 cut(s) 74, 358, 433
EheI GGCGCC 3 cut(s) 74, 358, 433
ErhI CCWWGG 4 cut(s) 68, 240, 405, 939
Esp3I CGTCTC 2 cut(s) 58, 211
FaeI CATG 7 cut(s) 72, 169, 190, 286, 925, 1169, 1264
FaqI GGGAC 3 cut(s) 13, 115, 637
FatI CATG 7 cut(s) 68, 165, 186, 282, 921, 1165, 1260
FauI CCCGC 5 cut(s) 128, 178, 664, 1049, 1151
FokI GGATG 2 cut(s) 296, 1094
FriOI GRGCYC 2 cut(s) 396, 549
FspI TGCGCA 1 cut(s) 507
GlaI GCGC 7 cut(s) 74, 358, 433, 507, 537, 888, 957
GsaI CCCAGC 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 1227
HaeII RGCGCY 4 cut(s) 76, 360, 435, 959
HaeIII GGCC 9 cut(s) 5, 404, 410, 449, 547, 563, 630, 1142, 1248
HapII CCGG 7 cut(s) 429, 539, 627, 749, 850, 863, 971
HgaI GACGC 1 cut(s) 241
HhaI GCGC 7 cut(s) 75, 359, 434, 508, 538, 889, 958
Hin1I GRCGYC 5 cut(s) 73, 231, 288, 357, 432
Hin1II CATG 7 cut(s) 72, 169, 190, 286, 925, 1169, 1264
Hin6I GCGC 7 cut(s) 73, 357, 432, 506, 536, 887, 956
HinP1I GCGC 7 cut(s) 73, 357, 432, 506, 536, 887, 956
HinfI GANTC 7 cut(s) 266, 341, 370, 425, 704, 829, 1308
HpaII CCGG 7 cut(s) 429, 539, 627, 749, 850, 863, 971
HphI GGTGA 5 cut(s) 127, 781, 877, 1346, 1348
Hpy166II GTNNAC 3 cut(s) 604, 868, 1219
Hpy188I TCNGA 2 cut(s) 223, 271
Hpy188III TCNNGA 5 cut(s) 292, 367, 637, 1079, 1179
Hpy8I GTNNAC 3 cut(s) 604, 868, 1219
Hpy99I CGWCG 9 cut(s) 233, 257, 260, 263, 266, 293, 308, 332, 356
HpyAV CCTTC 4 cut(s) 49, 87, 218, 1396
HpyCH4III ACNGT 5 cut(s) 139, 559, 778, 1019, 1288
HpyCH4IV ACGT 4 cut(s) 231, 288, 527, 657
HpyCH4V TGCA 6 cut(s) 799, 806, 990, 1085, 1301, 1403
HpyF3I CTNAG 4 cut(s) 176, 588, 963, 1177
HpySE526I ACGT 4 cut(s) 231, 288, 527, 657
Hsp92I GRCGYC 5 cut(s) 73, 231, 288, 357, 432
Hsp92II CATG 7 cut(s) 72, 169, 190, 286, 925, 1169, 1264
HspAI GCGC 7 cut(s) 73, 357, 432, 506, 536, 887, 956
KasI GGCGCC 3 cut(s) 72, 356, 431
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
KspI CCGCGG 1 cut(s) 672
Kzo9I GATC 5 cut(s) 150, 159, 201, 213, 1351
LmnI GCTCC 7 cut(s) 116, 185, 499, 520, 730, 1271, 1370
LweI GCATC 6 cut(s) 318, 865, 874, 1072, 1219, 1288
MaeII ACGT 4 cut(s) 231, 288, 527, 657
MaeIII GTNAC 4 cut(s) 457, 478, 916, 1342
MalI GATC 5 cut(s) 152, 161, 203, 215, 1353
MbiI CCGCTC 2 cut(s) 111, 1146
MboI GATC 5 cut(s) 150, 159, 201, 213, 1351
MboII GAAGA 9 cut(s) 39, 154, 410, 565, 589, 826, 1067, 1229, 1303
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 396, 549
MlsI TGGCCA 1 cut(s) 563
MluCI AATT 4 cut(s) 642, 675, 1198, 1359
MluNI TGGCCA 1 cut(s) 563
Mly113I GGCGCC 3 cut(s) 73, 357, 432
MlyI GAGTC 4 cut(s) 275, 350, 713, 838
MmeI TCCRAC 1 cut(s) 1249
Mox20I TGGCCA 1 cut(s) 563
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 563
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 1 cut(s) 563
MspA1I CMGCKG 4 cut(s) 452, 533, 671, 987
MspI CCGG 7 cut(s) 429, 539, 627, 749, 850, 863, 971
MspR9I CCNGG 8 cut(s) 211, 550, 749, 768, 851, 971, 1245, 1250
Mva1269I GAATGC 2 cut(s) 104, 985
MvaI CCWGG 5 cut(s) 211, 550, 768, 1245, 1250
MvnI CGCG 3 cut(s) 320, 437, 671
NaeI GCCGGC 1 cut(s) 540
NarI GGCGCC 3 cut(s) 73, 357, 432
NciI CCSGG 3 cut(s) 749, 851, 971
NcoI CCATGG 1 cut(s) 68
NdeII GATC 5 cut(s) 150, 159, 201, 213, 1351
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 7 cut(s) 72, 169, 190, 286, 925, 1169, 1264
NlaIV GGNNCC 8 cut(s) 74, 131, 358, 433, 547, 625, 726, 840
NmeAIII GCCGAG 2 cut(s) 403, 415
NmuCI GTSAC 1 cut(s) 916
NsbI TGCGCA 1 cut(s) 507
NspV TTCGAA 1 cut(s) 812
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 267, 336
PctI GAATGC 2 cut(s) 104, 985
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 370, 425, 1308
PfoI TCCNGGA 1 cut(s) 209
PleI GAGTC 4 cut(s) 274, 349, 712, 837
PluTI GGCGCC 3 cut(s) 76, 360, 435
PpsI GAGTC 4 cut(s) 274, 349, 712, 837
PpuMI RGGWCCY 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 129
Psp6I CCWGG 5 cut(s) 209, 548, 766, 1243, 1248
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 5 cut(s) 209, 548, 766, 1243, 1248
PspN4I GGNNCC 8 cut(s) 74, 131, 358, 433, 547, 625, 726, 840
PspOMI GGGCCC 1 cut(s) 545
PspPI GGNCC 6 cut(s) 129, 545, 546, 624, 629, 1141
PspPPI RGGWCCY 1 cut(s) 129
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 1 cut(s) 159
PvuII CAGCTG 2 cut(s) 533, 987
RsaI GTAC 1 cut(s) 1172
RsaNI GTAC 1 cut(s) 1171
RseI CAYNNNNRTG 1 cut(s) 794
SacI GAGCTC 1 cut(s) 396
SacII CCGCGG 1 cut(s) 672
Sau3AI GATC 5 cut(s) 150, 159, 201, 213, 1351
Sau96I GGNCC 6 cut(s) 129, 545, 546, 624, 629, 1141
SchI GAGTC 4 cut(s) 275, 350, 713, 838
ScrFI CCNGG 8 cut(s) 211, 550, 749, 768, 851, 971, 1245, 1250
SduI GDGCHC 2 cut(s) 396, 549
SfaNI GCATC 6 cut(s) 318, 865, 874, 1072, 1219, 1288
SfcI CTRYAG 2 cut(s) 699, 804
SfoI GGCGCC 3 cut(s) 74, 358, 433
Sfr303I CCGCGG 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 812
SgrBI CCGCGG 1 cut(s) 672
SinI GGWCC 2 cut(s) 129, 624
SmiMI CAYNNNNRTG 1 cut(s) 794
Sse9I AATT 4 cut(s) 642, 675, 1198, 1359
SspDI GGCGCC 3 cut(s) 72, 356, 431
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 8 cut(s) 209, 548, 747, 766, 849, 969, 1243, 1248
StyI CCWWGG 4 cut(s) 68, 240, 405, 939
TaaI ACNGT 5 cut(s) 139, 559, 778, 1019, 1288
TaiI ACGT 4 cut(s) 234, 291, 530, 660
TaqI TCGA 7 cut(s) 264, 291, 396, 773, 812, 882, 1227
TaqII GACCGA 1 cut(s) 206
TasI AATT 4 cut(s) 642, 675, 1198, 1359
TatI WGTACW 1 cut(s) 1170
TfiI GAWTC 3 cut(s) 370, 425, 1308
TscAI CASTG 1 cut(s) 1321
TseFI GTSAC 1 cut(s) 916
Tsp45I GTSAC 1 cut(s) 916
TspDTI ATGAA 1 cut(s) 154
TspGWI ACGGA 5 cut(s) 40, 682, 717, 1011, 1320
TspRI CASTG 1 cut(s) 1321
VpaK11BI GGWCC 2 cut(s) 129, 624
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 1 cut(s) 675
XcmI CCANNNNNNNNNTGG 2 cut(s) 556, 691
ZraI GACGTC 2 cut(s) 232, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.