pycom13g03820

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011790
Physical Location & Seq
Reverse (-)
2539038 .. 2541490
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03820.4

Sequence Viewer

Length: 1422 bp
ATGGCGTCGTCTCCTTTCCTCTCCAAGACCACCATTTCCTTCTCCTCCTCCATCTCCACCGCCCTCCCATGGCGCCCCTCCCCCTCCTCCTCCTCCACCGCATTCCGCTCCATTCCCCGCAGCCGCACCCTCACCGTCCAATCCAAGATCAGGGAGATCTTCATGCCTGCTCTCAGCTCCACTATGACCGAGGGCAAGATCGTCTCCTGGGTCAAATCCGAGGGCGACGTCCTCTCCAAAGGCGAGAGCGTCGTTGTTGTCGAGTCTGACAAAGCCGACATGGACGTAGAGACCTTCTACGACGGGATTCTCGCCGCAATCGTAGTCGGGGAAGGCGAGACCGCCCCCGTCGGCGCCGCCATCGGGCTTCTCGCTGAGACCGAAGAGGAAGTCGCCGAGGCCAGGGAAAAAGCCAAATCCACTTCGTCTTCTTCATCCCCAGCTGTTACGCCCACGCCTCCTCCTGCTACCGCCACTCCCGCCCCTGCAATTGCCCAGCCTGCTCCATCTGCTACTCCTACTTACACTGCCAAAAAGGGCGTGGCCACTCCTTTTGCTAAGAAGCTAGCCAAGCAGCACAAGGTCGAAATTGCCTCCGTGGTGGGCACCGGCCCATTTGGCCGAATTACGCCTGCTGACGTGGAGGCGGCCGCCGGAATTGTCAAGCCTGCTAAACCCGTCGCTGCCGAGCCCACTCCGGTGGCCGCTGCTCCACCCAAGCCTTCTGCTGCAGCCCCGCCTCTGCTTCCCGGCTCAACCGTGGTGCCATTTACCACGATGCAGGCTGCTGTTTCGAAGAACATGGTCGAAAGTCTCTCGGTGCCGACTTTCCGGGTCGGCTACCCGATTAACAGTGACGCCATCGACGCCCTTTATGAGAAGGTGAAACCAAAGGGTGTGACAATGACTGCATTGTTGGCCAAGGCAGCTGCTATGGCACTTGCTCAGCACCCGGTTGTGAATGCGAGCTGCAAAGACGGGAAGAGCTTTTCGTATAACAGCAGCATAAACATTGCTGTTGCTGTGGCAATCAATGGAGGACTCATAACCCCTGTTCTTCAGGATGCTGATAAGTTGGATTTGTATTTGCTATCCCAGAAATGGAAAGAGTTAGTGGAGAAGGCCCGTTCAAAGCAACTTCAGCCCCACGAGTACACAACAGGAACTTTCACCTTGTCCAATTTGGGCATGTTTGGAGTGGACAGGTTTGATGCTATTCTTCCACCAGGGCAGGGGGCTATCATGGCTGTTGGAGCTTCAAAGCCAACTGTTGTGGCTGATACAGATGGATTCTTCAGTGTAAAAAGTAAAATGCTGGTGAATGTAACTGCTGATCACCGGATTGTTTATGGTGCTGATTTGGCTGCCTTCCTCAAGACATTTGCAAAGATTGTTGAGAACCCAGAGAGCCTCACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

474

Amino Acids

49.21

Weight (kDa)

8.63

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25860
fragaria_vesca FvH4_4g27580
malus_domestica MD13G1043400.v1.1 MD16G1044200.v1.1
prunus_persica Prupe.1G309100_v2.0.a1
pyrus_communis pycom13g03820 pycom16g03840
rosa_chinensis RchiOBHm_Chr4g0435441
rosa_laevigata RLG00000006592
rosa_multiflora Rmu_sc0008521.1_g000001
rosa_roxburghii Rroxscaffold_5G00376680
rosa_rugosa Rorug04G0285000 Rorug04G0285100 Rorug05G0266800
rosa_samantha Rh4AG340800 Rh4BG349100 Rh4CG363600 Rh4DG343300
rosa_wichuraiana Rw4G029720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 231
AccB1I GGYRCC 5 cut(s) 72, 353, 605, 763, 820
AccBSI CCGCTC 1 cut(s) 108
AcoI YGGCCR 5 cut(s) 543, 619, 648, 702, 918
AcuI CTGAAG 3 cut(s) 1043, 1124, 1279
AcyI GRCGYC 6 cut(s) 5, 73, 228, 354, 858, 867
AfaI GTAC 1 cut(s) 1154
AfiI CCNNNNNNNGG 6 cut(s) 69, 150, 363, 402, 1101, 1232
AgsI TTSAA 2 cut(s) 1131, 1260
AjiI CACGTC 1 cut(s) 640
AjnI CCWGG 3 cut(s) 206, 401, 1225
AleI CACNNNNGTG 1 cut(s) 698
AluBI AGCT 7 cut(s) 177, 443, 565, 929, 969, 987, 1256
AluI AGCT 7 cut(s) 177, 443, 565, 929, 969, 987, 1256
Alw26I GTCTC 6 cut(s) 15, 208, 284, 332, 371, 818
AoxI GGCC 8 cut(s) 399, 543, 610, 619, 648, 702, 918, 1122
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
AspLEI GCGC 2 cut(s) 75, 356
AspS9I GGNCC 2 cut(s) 611, 1123
AsuC2I CCSGG 3 cut(s) 750, 833, 953
AsuHPI GGTGA 5 cut(s) 124, 895, 1162, 1328, 1330
AsuII TTCGAA 1 cut(s) 794
AsuNHI GCTAGC 1 cut(s) 565
BaeGI GKGCMC 1 cut(s) 608
BalI TGGCCA 2 cut(s) 545, 920
BanI GGYRCC 5 cut(s) 72, 353, 605, 763, 820
BanII GRGCYC 1 cut(s) 693
BauI CACGAG 1 cut(s) 1148
BbsI GAAGAC 1 cut(s) 420
BccI CCATC 5 cut(s) 59, 368, 514, 869, 1280
BciT130I CCWGG 3 cut(s) 208, 403, 1227
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 3 cut(s) 750, 833, 953
BcoDI GTCTC 6 cut(s) 15, 208, 284, 332, 371, 818
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 729
BfoI RGCGCY 2 cut(s) 76, 357
BglI GCCNNNNNGGC 1 cut(s) 618
BglII AGATCT 1 cut(s) 156
BlpI GCTNAGC 1 cut(s) 945
Bme1390I CCNGG 6 cut(s) 208, 403, 750, 833, 953, 1227
BmgBI CACGTC 1 cut(s) 640
BmgT120I GGNCC 2 cut(s) 611, 1123
BmiI GGNNCC 5 cut(s) 74, 355, 607, 765, 822
BmrFI CCNGG 6 cut(s) 208, 403, 750, 833, 953, 1227
BmsI GCATC 3 cut(s) 768, 1054, 1201
BmtI GCTAGC 1 cut(s) 569
BpiI GAAGAC 1 cut(s) 420
Bpu1102I GCTNAGC 1 cut(s) 945
Bpu14I TTCGAA 1 cut(s) 794
BpuEI CTTGAG 1 cut(s) 1358
BpuMI CCSGG 3 cut(s) 750, 833, 953
BsaHI GRCGYC 6 cut(s) 5, 73, 228, 354, 858, 867
BsaI GGTCTC 3 cut(s) 284, 332, 371
BsaWI WCCGGW 2 cut(s) 697, 1338
BsaXI ACNNNNNCTCC 6 cut(s) 218, 248, 445, 460, 475, 490
Bsc4I CCNNNNNNNGG 6 cut(s) 69, 150, 363, 402, 1101, 1232
Bse118I RCCGGY 1 cut(s) 608
Bse3DI GCAATG 1 cut(s) 1011
BseBI CCWGG 3 cut(s) 208, 403, 1227
BseGI GGATG 2 cut(s) 434, 1069
BseLI CCNNNNNNNGG 6 cut(s) 69, 150, 363, 402, 1101, 1232
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 3 cut(s) 187, 366, 959
BseRI GAGGAG 6 cut(s) 34, 37, 76, 79, 82, 450
BseSI GKGCMC 1 cut(s) 608
BseX3I CGGCCG 1 cut(s) 648
BseYI CCCAGC 2 cut(s) 439, 495
Bsh1285I CGRYCG 1 cut(s) 651
BshFI GGCC 8 cut(s) 401, 545, 612, 621, 650, 704, 920, 1124
BshNI GGYRCC 5 cut(s) 72, 353, 605, 763, 820
BsiEI CGRYCG 1 cut(s) 651
BsiSI CCGG 7 cut(s) 609, 654, 698, 750, 832, 953, 1339
BslI CCNNNNNNNGG 6 cut(s) 69, 150, 363, 402, 1101, 1232
BsmAI GTCTC 6 cut(s) 15, 208, 284, 332, 371, 818
BsmBI CGTCTC 2 cut(s) 15, 208
BsmI GAATGC 2 cut(s) 101, 967
BsnI GGCC 8 cut(s) 401, 545, 612, 621, 650, 704, 920, 1124
Bso31I GGTCTC 3 cut(s) 284, 332, 371
Bsp119I TTCGAA 1 cut(s) 794
Bsp1286I GDGCHC 2 cut(s) 608, 693
Bsp143I GATC 4 cut(s) 147, 156, 198, 1333
Bsp1720I GCTNAGC 1 cut(s) 945
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 8 cut(s) 401, 545, 612, 621, 650, 704, 920, 1124
BspCNI CTCAG 3 cut(s) 186, 367, 958
BspLI GGNNCC 5 cut(s) 74, 355, 607, 765, 822
BspMAI CTGCAG 1 cut(s) 733
BspOI GCTAGC 1 cut(s) 569
BspQI GCTCTTC 1 cut(s) 977
BspT104I TTCGAA 1 cut(s) 794
BspT107I GGYRCC 5 cut(s) 72, 353, 605, 763, 820
BspTNI GGTCTC 3 cut(s) 284, 332, 371
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 1011
BsrFI RCCGGY 1 cut(s) 608
BssAI RCCGGY 1 cut(s) 608
BssMI GATC 4 cut(s) 147, 156, 198, 1333
BssNI GRCGYC 6 cut(s) 5, 73, 228, 354, 858, 867
BssSI CACGAG 1 cut(s) 1148
BssT1I CCWWGG 2 cut(s) 68, 921
Bst2BI CACGAG 1 cut(s) 1148
Bst2UI CCWGG 3 cut(s) 208, 403, 1227
Bst4CI ACNGT 4 cut(s) 136, 760, 854, 1270
Bst6I CTCTTC 2 cut(s) 378, 977
BstACI GRCGYC 6 cut(s) 5, 73, 228, 354, 858, 867
BstBI TTCGAA 1 cut(s) 794
BstC8I GCNNGC 7 cut(s) 168, 501, 567, 633, 669, 783, 967
BstDEI CTNAG 4 cut(s) 173, 375, 558, 945
BstDSI CCRYGG 3 cut(s) 68, 597, 759
BstF5I GGATG 2 cut(s) 434, 1069
BstH2I RGCGCY 2 cut(s) 76, 357
BstHHI GCGC 2 cut(s) 75, 356
BstKTI GATC 4 cut(s) 150, 159, 201, 1336
BstMAI GTCTC 6 cut(s) 15, 208, 284, 332, 371, 818
BstMBI GATC 4 cut(s) 147, 156, 198, 1333
BstMCI CGRYCG 1 cut(s) 651
BstNI CCWGG 3 cut(s) 208, 403, 1227
BstNSI RCATGY 1 cut(s) 1192
BstSCI CCNGG 6 cut(s) 206, 401, 748, 831, 951, 1225
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 1 cut(s) 608
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 700
BstYI RGATCY 1 cut(s) 156
BstZI CGGCCG 1 cut(s) 648
BsuRI GGCC 8 cut(s) 401, 545, 612, 621, 650, 704, 920, 1124
BtgI CCRYGG 3 cut(s) 68, 597, 759
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 2 cut(s) 434, 1069
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 3 cut(s) 525, 859, 1303
Cac8I GCNNGC 7 cut(s) 168, 501, 567, 633, 669, 783, 967
CciNI GCGGCCGC 1 cut(s) 648
CfoI GCGC 2 cut(s) 75, 356
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 2 cut(s) 611, 1123
CseI GACGC 3 cut(s) 238, 866, 875
Csp6I GTAC 1 cut(s) 1153
CspCI CAANNNNNGTGG 4 cut(s) 535, 570, 1254, 1289
CviAII CATG 6 cut(s) 69, 163, 280, 802, 1189, 1243
CviQI GTAC 1 cut(s) 1153
DdeI CTNAG 4 cut(s) 173, 375, 558, 945
DinI GGCGCC 2 cut(s) 74, 355
DpnI GATC 4 cut(s) 149, 158, 200, 1335
DpnII GATC 4 cut(s) 147, 156, 198, 1333
EaeI YGGCCR 5 cut(s) 543, 619, 648, 702, 918
EagI CGGCCG 1 cut(s) 648
Eam1104I CTCTTC 2 cut(s) 378, 977
EarI CTCTTC 2 cut(s) 378, 977
EclXI CGGCCG 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 68, 921
Eco24I GRGCYC 1 cut(s) 693
Eco31I GGTCTC 3 cut(s) 284, 332, 371
Eco52I CGGCCG 1 cut(s) 648
Eco57I CTGAAG 3 cut(s) 1043, 1124, 1279
EcoRII CCWGG 3 cut(s) 206, 401, 1225
EcoT14I CCWWGG 2 cut(s) 68, 921
EcoT38I GRGCYC 1 cut(s) 693
EgeI GGCGCC 2 cut(s) 74, 355
EheI GGCGCC 2 cut(s) 74, 355
ErhI CCWWGG 2 cut(s) 68, 921
Esp3I CGTCTC 2 cut(s) 15, 208
FaeI CATG 6 cut(s) 72, 166, 283, 805, 1192, 1246
FatI CATG 6 cut(s) 68, 162, 279, 801, 1188, 1242
FauI CCCGC 3 cut(s) 125, 487, 744
FbaI TGATCA 1 cut(s) 1333
FokI GGATG 2 cut(s) 421, 1076
FriOI GRGCYC 1 cut(s) 693
FspBI CTAG 1 cut(s) 566
GlaI GCGC 2 cut(s) 74, 355
GsaI CCCAGC 2 cut(s) 443, 499
HaeII RGCGCY 2 cut(s) 76, 357
HaeIII GGCC 8 cut(s) 401, 545, 612, 621, 650, 704, 920, 1124
HapII CCGG 7 cut(s) 609, 654, 698, 750, 832, 953, 1339
HgaI GACGC 3 cut(s) 238, 866, 875
HhaI GCGC 2 cut(s) 75, 356
Hin1I GRCGYC 6 cut(s) 5, 73, 228, 354, 858, 867
Hin1II CATG 6 cut(s) 72, 166, 283, 805, 1192, 1246
Hin6I GCGC 2 cut(s) 73, 354
HinP1I GCGC 2 cut(s) 73, 354
HinfI GANTC 4 cut(s) 263, 307, 1041, 1290
HpaII CCGG 7 cut(s) 609, 654, 698, 750, 832, 953, 1339
HphI GGTGA 5 cut(s) 124, 895, 1162, 1328, 1330
Hpy166II GTNNAC 2 cut(s) 1155, 1201
Hpy188I TCNGA 2 cut(s) 220, 268
Hpy188III TCNNGA 2 cut(s) 1061, 1375
Hpy8I GTNNAC 2 cut(s) 1155, 1201
Hpy99I CGWCG 7 cut(s) 10, 230, 254, 305, 353, 683, 869
HpyAV CCTTC 7 cut(s) 49, 304, 326, 732, 874, 1114, 1378
HpyCH4III ACNGT 4 cut(s) 136, 760, 854, 1270
HpyCH4IV ACGT 3 cut(s) 228, 285, 639
HpyCH4V TGCA 6 cut(s) 488, 731, 781, 911, 972, 1385
HpyF3I CTNAG 4 cut(s) 173, 375, 558, 945
HpySE526I ACGT 3 cut(s) 228, 285, 639
Hsp92I GRCGYC 6 cut(s) 5, 73, 228, 354, 858, 867
Hsp92II CATG 6 cut(s) 72, 166, 283, 805, 1192, 1246
HspAI GCGC 2 cut(s) 73, 354
KasI GGCGCC 2 cut(s) 72, 353
Ksp22I TGATCA 1 cut(s) 1333
Kzo9I GATC 4 cut(s) 147, 156, 198, 1333
LguI GCTCTTC 1 cut(s) 977
LmnI GCTCC 5 cut(s) 113, 182, 508, 715, 1253
LweI GCATC 3 cut(s) 768, 1054, 1201
MaeI CTAG 1 cut(s) 566
MaeII ACGT 3 cut(s) 228, 285, 639
MaeIII GTNAC 4 cut(s) 445, 854, 898, 1324
MalI GATC 4 cut(s) 149, 158, 200, 1335
MbiI CCGCTC 1 cut(s) 108
MboI GATC 4 cut(s) 147, 156, 198, 1333
MboII GAAGA 9 cut(s) 151, 395, 420, 423, 808, 994, 1049, 1211, 1285
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 608, 693
MlsI TGGCCA 2 cut(s) 545, 920
MluCI AATT 5 cut(s) 489, 588, 624, 657, 1180
MluNI TGGCCA 2 cut(s) 545, 920
Mly113I GGCGCC 2 cut(s) 73, 354
MlyI GAGTC 2 cut(s) 272, 1035
MmeI TCCRAC 2 cut(s) 1056, 1231
Mox20I TGGCCA 2 cut(s) 545, 920
MscI TGGCCA 2 cut(s) 545, 920
MseI TTAA 1 cut(s) 849
MslI CAYNNNNRTG 1 cut(s) 698
Msp20I TGGCCA 2 cut(s) 545, 920
MspA1I CMGCKG 3 cut(s) 443, 707, 929
MspI CCGG 7 cut(s) 609, 654, 698, 750, 832, 953, 1339
MspR9I CCNGG 6 cut(s) 208, 403, 750, 833, 953, 1227
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 2 cut(s) 101, 967
MvaI CCWGG 3 cut(s) 208, 403, 1227
NarI GGCGCC 2 cut(s) 73, 354
NciI CCSGG 3 cut(s) 750, 833, 953
NcoI CCATGG 1 cut(s) 68
NdeII GATC 4 cut(s) 147, 156, 198, 1333
NheI GCTAGC 1 cut(s) 565
NlaIII CATG 6 cut(s) 72, 166, 283, 805, 1192, 1246
NlaIV GGNNCC 5 cut(s) 74, 355, 607, 765, 822
NmeAIII GCCGAG 2 cut(s) 421, 712
NmuCI GTSAC 2 cut(s) 854, 898
NotI GCGGCCGC 1 cut(s) 648
NspI RCATGY 1 cut(s) 1192
NspV TTCGAA 1 cut(s) 794
OliI CACNNNNGTG 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 977
PcsI WCGNNNNNNNCGW 4 cut(s) 258, 318, 333, 378
PctI GAATGC 2 cut(s) 101, 967
PfeI GAWTC 2 cut(s) 307, 1290
PleI GAGTC 2 cut(s) 271, 1035
PluTI GGCGCC 2 cut(s) 76, 357
PpsI GAGTC 2 cut(s) 271, 1035
Psp6I CCWGG 3 cut(s) 206, 401, 1225
PspFI CCCAGC 2 cut(s) 439, 495
PspGI CCWGG 3 cut(s) 206, 401, 1225
PspN4I GGNNCC 5 cut(s) 74, 355, 607, 765, 822
PspPI GGNCC 2 cut(s) 611, 1123
PstI CTGCAG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 156
PvuII CAGCTG 2 cut(s) 443, 929
RsaI GTAC 1 cut(s) 1154
RsaNI GTAC 1 cut(s) 1153
RseI CAYNNNNRTG 1 cut(s) 698
SapI GCTCTTC 1 cut(s) 977
SaqAI TTAA 1 cut(s) 849
Sau3AI GATC 4 cut(s) 147, 156, 198, 1333
Sau96I GGNCC 2 cut(s) 611, 1123
SchI GAGTC 2 cut(s) 272, 1035
ScrFI CCNGG 6 cut(s) 208, 403, 750, 833, 953, 1227
SduI GDGCHC 2 cut(s) 608, 693
SfaNI GCATC 3 cut(s) 768, 1054, 1201
SfcI CTRYAG 1 cut(s) 729
SfiI GGCCNNNNNGGCC 1 cut(s) 618
SfoI GGCGCC 2 cut(s) 74, 355
SfuI TTCGAA 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 1 cut(s) 1373
SmoI CTYRAG 1 cut(s) 1373
Sse9I AATT 5 cut(s) 489, 588, 624, 657, 1180
SspDI GGCGCC 2 cut(s) 72, 353
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 6 cut(s) 206, 401, 748, 831, 951, 1225
StyI CCWWGG 2 cut(s) 68, 921
TaaI ACNGT 4 cut(s) 136, 760, 854, 1270
TaiI ACGT 3 cut(s) 231, 288, 642
TaqI TCGA 5 cut(s) 261, 585, 794, 807, 864
TaqII GACCGA 2 cut(s) 203, 395
TasI AATT 5 cut(s) 489, 588, 624, 657, 1180
TatI WGTACW 1 cut(s) 1152
TauI GCSGC 6 cut(s) 126, 317, 359, 650, 653, 707
TfiI GAWTC 2 cut(s) 307, 1290
Tru1I TTAA 1 cut(s) 849
Tru9I TTAA 1 cut(s) 849
TscAI CASTG 3 cut(s) 532, 859, 1303
TseFI GTSAC 2 cut(s) 854, 898
Tsp45I GTSAC 2 cut(s) 854, 898
TspDTI ATGAA 2 cut(s) 151, 423
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 3 cut(s) 532, 859, 1303
XceI RCATGY 1 cut(s) 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XspI CTAG 1 cut(s) 566
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.