Rw4G029720

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011790
Physical Location & Seq
Reverse (-)
56438860 .. 56441743
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029720.1

Sequence Viewer

Length: 1431 bp
ATGGCCTCCTCTCCTTTCCTCTCCAAGCCCACCATTTCATTCTCCTCCTCCGTCTCCACCTCCCTCCCATGGCGGCCCTCCCTCTCCTCCTCCGCATTTCGCGCCAACCCCCGCCGCAGAACCCTGACCGTCCAATCCAAGATCAGGGAGATCTTCATGCCCGCCCTCAGCTCCACCATGACCGAGGGCAAGATCGTCTCCTGGATCAAATCCGAGGGCGACGTCCTCTCCAAGGGCGAGAGCGTCGTCGTTGTAGAGTCCGACAAGGCTGATATGGACGTCGAGACTTTCTACGACGGCATCCTCGCCGCGATTGTCGTCGGCGAGGGCGAGACCGCCCCCGTCGGCGCCGCCATCGGAATTCTCGCCGAGACTGAAGCCGAGCTCGATGAGGCCAAGGCTAAAGCCAAAGCCGCCGATTCCGGTGCCTCTAAGCCCGAGTCCAGTGCCGTTACGCCCACTCCTCCTCCTGCTACTGCCACTCCTGCACCTGCTATAGCGCAGCCTGCCACGTCAGCGGCTCCAGCTGGGCCCAGGAAGACTGTGGCTACCCCCTTTGCGAAGAAGCTAGCTAAGCAACACAAGGTGGACATTGCCACCGTCGTCGGCACCGGCCCATTCGGCAGAATTACTCCTGCTGACGTGGAGGCTGCCGCCGGAATTGAGCCGAAGAAAACCGTGGCTGCCGTGCCTACTCCGGCTGCGGCGGCTCCGGCCAAGTCTGCTGCTTCTTCCGCTCCTGCATTGCCTCTACTTCCAGGTTCCACTGTCGTGCCTTTCACCACAATGCAGGCTGCAGTCTCGAAGAACATGATCGAAAGTCTCTCGGTGCCGACGTTCCGGGTGGGCTATCCGGTGAACAGCGATGCCATAGATGCGATGTATGATAAGGTGAAACGAAAGGGTGTGACCATGACTGCCTTGCTTGCCAAGGCGGTTGCTATGGCACTTGCTAAGCACCCGGTTGTGAATGCCAGCTGCAAAGATGGGAAGAGCTTTTACTACAACAGTAGCATAAACATCGCCGTTGCTGTGGCTATTGATGGTGGGCTGATAACCCCTGTTCTTCAGGATGCAGATAAGGTGGATTTGTATTTGTTATCTCAGAAGTGGAAAGAGCTGGTGGAGAAGGCTCGCTCAAAGCAACTTCAGCCCCATGAGTACAACTCAGGAACTTTCACTCTCTCCAATTTGGGAATGTTTGGAGTGGACAGATTCGATGCTATTCTTCCTCCAGGCCAAGGTGCTATCATGGCTGTTGGAGCTTCAAAACCCACTGTTGTAGCTGATGCTGACGGATTCTTCAGTGTAAAAAGTAAAATGCTGGTGAATGTAACAGCTGATCACCGAATTGTTTATGGAGCTGATTTGGCTGCCTTCCTACAGACATTTGCAAAGATTGTTGAGAACCCAGAAAGCCTGACAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

476

Amino Acids

49.5

Weight (kDa)

8.81

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 50 - 120 3.6e-14 Biotin-requiring enzyme
E3_binding PF02817 182 - 216 1.6e-13 e3 binding domain
2-oxoacid_dh PF00198 255 - 475 5.8e-64 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25860
fragaria_vesca FvH4_4g27580
malus_domestica MD13G1043400.v1.1 MD16G1044200.v1.1
prunus_persica Prupe.1G309100_v2.0.a1
pyrus_communis pycom13g03820 pycom16g03840
rosa_chinensis RchiOBHm_Chr4g0435441
rosa_laevigata RLG00000006592
rosa_multiflora Rmu_sc0008521.1_g000001
rosa_roxburghii Rroxscaffold_5G00376680
rosa_rugosa Rorug04G0285000 Rorug04G0285100 Rorug05G0266800
rosa_samantha Rh4AG340800 Rh4BG349100 Rh4CG363600 Rh4DG343300
rosa_wichuraiana Rw4G029720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 499
AatII GACGTC 2 cut(s) 225, 282
Acc36I ACCTGC 1 cut(s) 499
AccB1I GGYRCC 4 cut(s) 347, 425, 608, 829
AccBSI CCGCTC 1 cut(s) 737
AccII CGCG 2 cut(s) 102, 311
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 1 cut(s) 360
AcuI CTGAAG 4 cut(s) 396, 1052, 1133, 1288
AcyI GRCGYC 3 cut(s) 222, 279, 348
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 1 cut(s) 1163
AfiI CCNNNNNNNGG 4 cut(s) 69, 144, 232, 1241
AgsI TTSAA 1 cut(s) 1269
AjiI CACGTC 2 cut(s) 513, 643
AjnI CCWGG 4 cut(s) 200, 533, 757, 1234
AleI CACNNNNGTG 1 cut(s) 770
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 7 cut(s) 58, 202, 278, 326, 365, 805, 827
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 7 cut(s) 3, 74, 393, 530, 613, 714, 1237
ApaI GGGCCC 1 cut(s) 534
ApeKI GCWGC 8 cut(s) 502, 650, 683, 701, 725, 794, 978, 1373
ApoI RAATTY 1 cut(s) 360
AspLEI GCGC 3 cut(s) 104, 350, 502
AspS9I GGNCC 4 cut(s) 75, 530, 531, 614
AsuC2I CCSGG 2 cut(s) 842, 962
AsuHPI GGTGA 5 cut(s) 772, 868, 904, 1337, 1339
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 1 cut(s) 534
BanI GGYRCC 4 cut(s) 347, 425, 608, 829
BanII GRGCYC 2 cut(s) 387, 534
BarI GAAGNNNNNNTAC 2 cut(s) 983, 1015
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 167
BbvI GCAGC 8 cut(s) 514, 637, 670, 688, 712, 781, 965, 1360
BccI CCATC 3 cut(s) 362, 980, 1037
BceAI ACGGC 4 cut(s) 313, 434, 671, 1010
BcgI CGANNNNNNTGC 6 cut(s) 407, 428, 441, 462, 1003, 1037
BciT130I CCWGG 4 cut(s) 202, 535, 759, 1236
BclI TGATCA 1 cut(s) 1342
BcnI CCSGG 2 cut(s) 842, 962
BcoDI GTCTC 7 cut(s) 58, 202, 278, 326, 365, 805, 827
BfaI CTAG 1 cut(s) 569
BfmI CTRYAG 3 cut(s) 495, 795, 1382
BfoI RGCGCY 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 499
BglI GCCNNNNNGGC 1 cut(s) 621
BglII AGATCT 1 cut(s) 150
BlpI GCTNAGC 2 cut(s) 573, 954
Bme1390I CCNGG 6 cut(s) 202, 535, 759, 842, 962, 1236
BmeT110I CYCGRG 1 cut(s) 437
BmgBI CACGTC 2 cut(s) 513, 643
BmgT120I GGNCC 4 cut(s) 75, 530, 531, 614
BmiI GGNNCC 8 cut(s) 349, 427, 522, 532, 610, 711, 763, 831
BmrFI CCNGG 6 cut(s) 202, 535, 759, 842, 962, 1236
BmsI GCATC 6 cut(s) 309, 856, 865, 1063, 1210, 1279
BmtI GCTAGC 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
BpmI CTGGAG 2 cut(s) 507, 1218
Bpu10I CCTNAGC 1 cut(s) 167
Bpu1102I GCTNAGC 2 cut(s) 573, 954
BpuMI CCSGG 2 cut(s) 842, 962
BsaHI GRCGYC 3 cut(s) 222, 279, 348
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 9 cut(s) 68, 183, 213, 231, 396, 533, 678, 930, 1240
BsaWI WCCGGW 2 cut(s) 422, 853
BsaXI ACNNNNNCTCC 8 cut(s) 212, 242, 445, 451, 466, 475, 481, 496
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 144, 232, 1241
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 1 cut(s) 444
Bse3DI GCAATG 2 cut(s) 591, 743
BseBI CCWGG 4 cut(s) 202, 535, 759, 1236
BseDI CCNNGG 9 cut(s) 68, 183, 213, 231, 396, 533, 678, 930, 1240
BseGI GGATG 2 cut(s) 300, 1078
BseLI CCNNNNNNNGG 4 cut(s) 69, 144, 232, 1241
BseMI GCAATG 2 cut(s) 591, 743
BseMII CTCAG 3 cut(s) 181, 1118, 1182
BseNI ACTGG 1 cut(s) 444
BseRI GAGGAG 6 cut(s) 34, 37, 76, 79, 453, 456
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 8 cut(s) 514, 637, 670, 688, 712, 781, 965, 1360
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 471
Bsh1236I CGCG 2 cut(s) 102, 311
BshFI GGCC 7 cut(s) 5, 76, 395, 532, 615, 716, 1239
BshNI GGYRCC 4 cut(s) 347, 425, 608, 829
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 8 cut(s) 423, 612, 657, 698, 713, 841, 854, 962
BslI CCNNNNNNNGG 4 cut(s) 69, 144, 232, 1241
BsmAI GTCTC 7 cut(s) 58, 202, 278, 326, 365, 805, 827
BsmBI CGTCTC 2 cut(s) 58, 202
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 7 cut(s) 5, 76, 395, 532, 615, 716, 1239
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 437
Bsp120I GGGCCC 1 cut(s) 530
Bsp1286I GDGCHC 2 cut(s) 387, 534
Bsp143I GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
Bsp1720I GCTNAGC 2 cut(s) 573, 954
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 7 cut(s) 5, 76, 395, 532, 615, 716, 1239
BspCNI CTCAG 3 cut(s) 180, 1117, 1181
BspFNI CGCG 2 cut(s) 102, 311
BspLI GGNNCC 8 cut(s) 349, 427, 522, 532, 610, 711, 763, 831
BspMAI CTGCAG 1 cut(s) 799
BspMI ACCTGC 1 cut(s) 499
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 986
BspT107I GGYRCC 4 cut(s) 347, 425, 608, 829
BspTNI GGTCTC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 591, 743
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 1 cut(s) 444
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 9 cut(s) 68, 183, 213, 231, 396, 533, 678, 930, 1240
BssMI GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
BssNI GRCGYC 3 cut(s) 222, 279, 348
BssT1I CCWWGG 5 cut(s) 68, 231, 396, 930, 1240
Bst2UI CCWGG 4 cut(s) 202, 535, 759, 1236
Bst4CI ACNGT 7 cut(s) 130, 544, 601, 679, 769, 1010, 1279
Bst6I CTCTTC 1 cut(s) 986
BstACI GRCGYC 3 cut(s) 222, 279, 348
BstC8I GCNNGC 7 cut(s) 162, 507, 570, 792, 927, 976, 1135
BstDEI CTNAG 6 cut(s) 167, 432, 573, 954, 1104, 1168
BstDSI CCRYGG 2 cut(s) 68, 678
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 2 cut(s) 300, 1078
BstFNI CGCG 2 cut(s) 102, 311
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 3 cut(s) 104, 350, 502
BstKTI GATC 6 cut(s) 144, 153, 195, 207, 816, 1345
BstMAI GTCTC 7 cut(s) 58, 202, 278, 326, 365, 805, 827
BstMBI GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
BstNI CCWGG 4 cut(s) 202, 535, 759, 1236
BstSCI CCNGG 6 cut(s) 200, 533, 757, 840, 960, 1234
BstSFI CTRYAG 3 cut(s) 495, 795, 1382
BstSLI GKGCMC 1 cut(s) 534
BstUI CGCG 2 cut(s) 102, 311
BstV1I GCAGC 8 cut(s) 514, 637, 670, 688, 712, 781, 965, 1360
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 7 cut(s) 5, 76, 395, 532, 615, 716, 1239
BtgI CCRYGG 2 cut(s) 68, 678
BtgZI GCGATG 3 cut(s) 879, 893, 1006
BtrI CACGTC 2 cut(s) 513, 643
BtsCI GGATG 2 cut(s) 300, 1078
BtsIMutI CAGTG 4 cut(s) 451, 765, 1275, 1312
BveI ACCTGC 1 cut(s) 499
Cac8I GCNNGC 7 cut(s) 162, 507, 570, 792, 927, 976, 1135
CfoI GCGC 3 cut(s) 104, 350, 502
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 4 cut(s) 75, 530, 531, 614
CseI GACGC 1 cut(s) 232
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 7 cut(s) 69, 157, 178, 811, 913, 1157, 1252
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 6 cut(s) 167, 432, 573, 954, 1104, 1168
DinI GGCGCC 1 cut(s) 349
DpnI GATC 6 cut(s) 143, 152, 194, 206, 815, 1344
DpnII GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
DraIII CACNNNGTG 1 cut(s) 586
EaeI YGGCCR 1 cut(s) 714
Eam1104I CTCTTC 1 cut(s) 986
EarI CTCTTC 1 cut(s) 986
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 5 cut(s) 68, 231, 396, 930, 1240
Eco24I GRGCYC 2 cut(s) 387, 534
Eco31I GGTCTC 1 cut(s) 326
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 4 cut(s) 396, 1052, 1133, 1288
Eco88I CYCGRG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 360
EcoRII CCWGG 4 cut(s) 200, 533, 757, 1234
EcoT14I CCWWGG 5 cut(s) 68, 231, 396, 930, 1240
EcoT38I GRGCYC 2 cut(s) 387, 534
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 5 cut(s) 68, 231, 396, 930, 1240
Esp3I CGTCTC 2 cut(s) 58, 202
FaeI CATG 7 cut(s) 72, 160, 181, 814, 916, 1160, 1255
FatI CATG 7 cut(s) 68, 156, 177, 810, 912, 1156, 1251
FauI CCCGC 2 cut(s) 119, 169
FbaI TGATCA 1 cut(s) 1342
FokI GGATG 2 cut(s) 287, 1085
FriOI GRGCYC 2 cut(s) 387, 534
FspBI CTAG 1 cut(s) 569
GlaI GCGC 3 cut(s) 103, 349, 501
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 2 cut(s) 507, 1218
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 7 cut(s) 5, 76, 395, 532, 615, 716, 1239
HapII CCGG 8 cut(s) 423, 612, 657, 698, 713, 841, 854, 962
HgaI GACGC 1 cut(s) 232
HhaI GCGC 3 cut(s) 104, 350, 502
Hin1I GRCGYC 3 cut(s) 222, 279, 348
Hin1II CATG 7 cut(s) 72, 160, 181, 814, 916, 1160, 1255
Hin6I GCGC 3 cut(s) 102, 348, 500
HinP1I GCGC 3 cut(s) 102, 348, 500
HinfI GANTC 5 cut(s) 257, 419, 440, 1215, 1299
HpaII CCGG 8 cut(s) 423, 612, 657, 698, 713, 841, 854, 962
HphI GGTGA 5 cut(s) 772, 868, 904, 1337, 1339
Hpy166II GTNNAC 3 cut(s) 589, 859, 1210
Hpy188I TCNGA 4 cut(s) 214, 262, 359, 1107
Hpy188III TCNNGA 4 cut(s) 283, 802, 1070, 1170
Hpy8I GTNNAC 3 cut(s) 589, 859, 1210
HpyAV CCTTC 2 cut(s) 1123, 1387
HpyCH4III ACNGT 7 cut(s) 130, 544, 601, 679, 769, 1010, 1279
HpyCH4IV ACGT 5 cut(s) 222, 279, 512, 642, 836
HpyCH4V TGCA 7 cut(s) 488, 743, 790, 797, 981, 1076, 1394
HpyF3I CTNAG 6 cut(s) 167, 432, 573, 954, 1104, 1168
HpySE526I ACGT 5 cut(s) 222, 279, 512, 642, 836
Hsp92I GRCGYC 3 cut(s) 222, 279, 348
Hsp92II CATG 7 cut(s) 72, 160, 181, 814, 916, 1160, 1255
HspAI GCGC 3 cut(s) 102, 348, 500
KasI GGCGCC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 1342
Kzo9I GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
LguI GCTCTTC 1 cut(s) 986
LmnI GCTCC 6 cut(s) 176, 526, 715, 742, 1262, 1361
Lsp1109I GCAGC 8 cut(s) 514, 637, 670, 688, 712, 781, 965, 1360
LweI GCATC 6 cut(s) 309, 856, 865, 1063, 1210, 1279
MaeI CTAG 1 cut(s) 569
MaeII ACGT 5 cut(s) 222, 279, 512, 642, 836
MaeIII GTNAC 3 cut(s) 451, 907, 1333
MalI GATC 6 cut(s) 143, 152, 194, 206, 815, 1344
MbiI CCGCTC 1 cut(s) 737
MboI GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 2 cut(s) 387, 534
MluCI AATT 5 cut(s) 360, 627, 660, 1189, 1350
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 2 cut(s) 266, 449
MmeI TCCRAC 2 cut(s) 285, 1240
MslI CAYNNNNRTG 2 cut(s) 770, 785
MspA1I CMGCKG 4 cut(s) 518, 527, 978, 1340
MspI CCGG 8 cut(s) 423, 612, 657, 698, 713, 841, 854, 962
MspR9I CCNGG 6 cut(s) 202, 535, 759, 842, 962, 1236
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 4 cut(s) 202, 535, 759, 1236
MvnI CGCG 2 cut(s) 102, 311
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 2 cut(s) 842, 962
NcoI CCATGG 1 cut(s) 68
NdeII GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 7 cut(s) 72, 160, 181, 814, 916, 1160, 1255
NlaIV GGNNCC 8 cut(s) 349, 427, 522, 532, 610, 711, 763, 831
NmeAIII GCCGAG 2 cut(s) 394, 406
NmuCI GTSAC 1 cut(s) 907
OliI CACNNNNGTG 1 cut(s) 770
PaqCI CACCTGC 1 cut(s) 499
PciSI GCTCTTC 1 cut(s) 986
PcsI WCGNNNNNNNCGW 2 cut(s) 327, 833
PctI GAATGC 1 cut(s) 976
PfeI GAWTC 3 cut(s) 419, 1215, 1299
PfoI TCCNGGA 1 cut(s) 200
PleI GAGTC 2 cut(s) 265, 448
PluTI GGCGCC 1 cut(s) 351
PpsI GAGTC 2 cut(s) 265, 448
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 4 cut(s) 200, 533, 757, 1234
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 4 cut(s) 200, 533, 757, 1234
PspN4I GGNNCC 8 cut(s) 349, 427, 522, 532, 610, 711, 763, 831
PspOMI GGGCCC 1 cut(s) 530
PspPI GGNCC 4 cut(s) 75, 530, 531, 614
PstI CTGCAG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 150
PvuII CAGCTG 3 cut(s) 527, 978, 1340
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
RseI CAYNNNNRTG 2 cut(s) 770, 785
SacI GAGCTC 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 986
Sau3AI GATC 6 cut(s) 141, 150, 192, 204, 813, 1342
Sau96I GGNCC 4 cut(s) 75, 530, 531, 614
SchI GAGTC 2 cut(s) 266, 449
ScrFI CCNGG 6 cut(s) 202, 535, 759, 842, 962, 1236
SduI GDGCHC 2 cut(s) 387, 534
SfaNI GCATC 6 cut(s) 309, 856, 865, 1063, 1210, 1279
SfcI CTRYAG 3 cut(s) 495, 795, 1382
SfoI GGCGCC 1 cut(s) 349
SmiMI CAYNNNNRTG 2 cut(s) 770, 785
Sse9I AATT 5 cut(s) 360, 627, 660, 1189, 1350
SspDI GGCGCC 1 cut(s) 347
SspMI CTAG 1 cut(s) 569
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 6 cut(s) 200, 533, 757, 840, 960, 1234
StyI CCWWGG 5 cut(s) 68, 231, 396, 930, 1240
TaaI ACNGT 7 cut(s) 130, 544, 601, 679, 769, 1010, 1279
TaiI ACGT 5 cut(s) 225, 282, 515, 645, 839
TaqI TCGA 5 cut(s) 282, 387, 803, 816, 1218
TaqII GACCGA 1 cut(s) 197
TasI AATT 5 cut(s) 360, 627, 660, 1189, 1350
TatI WGTACW 1 cut(s) 1161
TauI GCSGC 9 cut(s) 76, 117, 311, 353, 416, 521, 656, 707, 710
TfiI GAWTC 3 cut(s) 419, 1215, 1299
TscAI CASTG 4 cut(s) 451, 772, 1282, 1312
TseFI GTSAC 1 cut(s) 907
TseI GCWGC 8 cut(s) 502, 650, 683, 701, 725, 794, 978, 1373
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 2 cut(s) 27, 145
TspGWI ACGGA 2 cut(s) 40, 1311
TspRI CASTG 4 cut(s) 451, 772, 1282, 1312
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XspI CTAG 1 cut(s) 569
ZraI GACGTC 2 cut(s) 223, 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.