MD13G1043400.v1.1

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011790
Physical Location & Seq
Reverse (-)
3037251 .. 3040591
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1043400.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGGCGTCGTCTCCTTTCCTCTCCAAGACCACCATTTCCTTCTCCTCCTCCATCTCCACAGCTCTCCCATGGCGCCCCTCCCCCTCCTCCTCCTCCTCTTCCACTGCATTCCGCTCCATTCCTCGCCGCCGCACCCTCACCGTCCAATCCAAGATCAGGGAGATCTTCATGCCTGCTCTCAGCTCCACTATGACCGAGGGAAAGATCGTCTCCTGGGTCAAATCCGAGGGCGACGTCCTCTCCAAAGGCGAGAGCGTCGTTGTCGTCGAGTCTGACAAAGCCGACATGGACGTAGAGACATTCTACGACGGGATTCTCGCCGCAATCGTCGTCGGGGAAGGCGAGACCGCTCCCGTCGGCGCCGCCATCGGGCTTCTCGCTGAGACCGAAGAGGAAGTCGCCGAGGCCAGGGAAAAGGCCAAATCCACATCGTCTTCTTCATCCCCAGCTGTTACGCCCACGCCTCCTCCTGCTACCGCCACTCCCGCCCCTGCAATTGCCCAGCCTGCTCCATCTGCTACTCCTACTTACACTGCCAAAAAGGGCGTGGCCACTCCTTTTGCTAAGAAGCTAGCCAAGCAGCACAAGGTCGAAATTGCCTCCGTGGTGGGCACCGGCCCATTTGGCCGAATTACGCCTGCTGACGTGGAGGCAGCCGCCGGAATTGTAAAGCCTGCTAAACCCGTCGCCGCCGAGCCCACTCCGGTGGCCGCTGCTCCACCCAAGCCTTCTGCTGCAGCCCCGCCTCTGCTTCCCGGCTCAACCGTGGTGCCATTTACCACGATGCAGGTTGCTGTTTCGAAGAACATGGTCGAAAGTCTCTCGGTGCCGACTTTCCGGGTCGGCTACCCGATTAACAGTGACGCCATCGACGCCCTTTATGAGAAGGTGAAACCAAAGGGTGTGACAATGACTGCATTGTTGGCCAAGGCTGCTGCTATGGCACTTGCTCAGCACCCGGTTGTGAATGCGAGCTGCAAAGACGGGAAGAGCTTTTCGTATAACAGCAGCATAAACATTGCTGTTGCTGTGGCAATCAATGGAGGACTCATAACCCCTGTTCTTCAGGATGCTGATAAGTTGGATTTGTATTTGCTATCCCAGAAATGGAAAGAGTTAGTGGAGAAGGCCCGTTCAAAGCAACTTCAGCCCCACGAATACACAACAGGAACTTTCACCTTGTCCAATTTGGGCATGTTTGGAGTGGACAGGTTTGATGCTATTCTTCCACCAGGGCAGGGGGCTATCATGGCTGTTGGAGCTTCAAAGCCAACTGTTGTCGCTGATACAGATGGATTCTTCAGTGTAAAAAGTAAAATGCTGGTGAATGTAACTGCTGATCACCGGATTGTTTATGGTGCTGATTTGGCTGCCTTCCTCAAGACATTTGCAAAGATTGTTGAGAACCCAGAGAGCCTCACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

476

Amino Acids

49.48

Weight (kDa)

8.82

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 54 - 123 6.4e-14 Biotin-requiring enzyme
E3_binding PF02817 184 - 217 4.3e-12 e3 binding domain
2-oxoacid_dh PF00198 254 - 474 1.4e-63 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25860
fragaria_vesca FvH4_4g27580
malus_domestica MD13G1043400.v1.1 MD16G1044200.v1.1
prunus_persica Prupe.1G309100_v2.0.a1
pyrus_communis pycom13g03820 pycom16g03840
rosa_chinensis RchiOBHm_Chr4g0435441
rosa_laevigata RLG00000006592
rosa_multiflora Rmu_sc0008521.1_g000001
rosa_roxburghii Rroxscaffold_5G00376680
rosa_rugosa Rorug04G0285000 Rorug04G0285100 Rorug05G0266800
rosa_samantha Rh4AG340800 Rh4BG349100 Rh4CG363600 Rh4DG343300
rosa_wichuraiana Rw4G029720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 778
AccB1I GGYRCC 5 cut(s) 72, 359, 611, 769, 826
AccBSI CCGCTC 2 cut(s) 114, 350
AcoI YGGCCR 4 cut(s) 549, 625, 708, 924
AcuI CTGAAG 3 cut(s) 1049, 1130, 1285
AcyI GRCGYC 6 cut(s) 5, 73, 234, 360, 864, 873
AfiI CCNNNNNNNGG 5 cut(s) 156, 369, 408, 1107, 1238
AgsI TTSAA 2 cut(s) 1137, 1266
AjiI CACGTC 1 cut(s) 646
AjnI CCWGG 3 cut(s) 212, 407, 1231
AleI CACNNNNGTG 1 cut(s) 704
AluBI AGCT 7 cut(s) 62, 183, 449, 571, 975, 993, 1262
AluI AGCT 7 cut(s) 62, 183, 449, 571, 975, 993, 1262
Alw26I GTCTC 6 cut(s) 15, 214, 290, 338, 377, 824
AoxI GGCC 8 cut(s) 405, 417, 549, 616, 625, 708, 924, 1128
ArsI GACNNNNNNTTYG 2 cut(s) 381, 413
AspLEI GCGC 2 cut(s) 75, 362
AspS9I GGNCC 2 cut(s) 617, 1129
AsuC2I CCSGG 3 cut(s) 756, 839, 959
AsuHPI GGTGA 5 cut(s) 130, 901, 1168, 1334, 1336
AsuII TTCGAA 1 cut(s) 800
AsuNHI GCTAGC 1 cut(s) 571
BaeGI GKGCMC 1 cut(s) 614
BalI TGGCCA 2 cut(s) 551, 926
BanI GGYRCC 5 cut(s) 72, 359, 611, 769, 826
BanII GRGCYC 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 426
BccI CCATC 5 cut(s) 59, 374, 520, 875, 1286
BciT130I CCWGG 3 cut(s) 214, 409, 1233
BclI TGATCA 1 cut(s) 1339
BcnI CCSGG 3 cut(s) 756, 839, 959
BcoDI GTCTC 6 cut(s) 15, 214, 290, 338, 377, 824
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 2 cut(s) 76, 363
BfuAI ACCTGC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 624
BglII AGATCT 1 cut(s) 162
BlpI GCTNAGC 1 cut(s) 951
Bme1390I CCNGG 6 cut(s) 214, 409, 756, 839, 959, 1233
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 617, 1129
BmiI GGNNCC 5 cut(s) 74, 361, 613, 771, 828
BmrFI CCNGG 6 cut(s) 214, 409, 756, 839, 959, 1233
BmsI GCATC 3 cut(s) 774, 1060, 1207
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 800
BpuEI CTTGAG 1 cut(s) 1364
BpuMI CCSGG 3 cut(s) 756, 839, 959
BsaHI GRCGYC 6 cut(s) 5, 73, 234, 360, 864, 873
BsaI GGTCTC 2 cut(s) 338, 377
BsaWI WCCGGW 2 cut(s) 703, 1344
BsaXI ACNNNNNCTCC 6 cut(s) 224, 254, 451, 466, 481, 496
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 369, 408, 1107, 1238
Bse118I RCCGGY 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 1017
BseBI CCWGG 3 cut(s) 214, 409, 1233
BseGI GGATG 2 cut(s) 440, 1075
BseLI CCNNNNNNNGG 5 cut(s) 156, 369, 408, 1107, 1238
BseMI GCAATG 1 cut(s) 1017
BseMII CTCAG 3 cut(s) 193, 372, 965
BseRI GAGGAG 7 cut(s) 34, 37, 76, 79, 82, 85, 456
BseSI GKGCMC 1 cut(s) 614
BseYI CCCAGC 2 cut(s) 445, 501
BshFI GGCC 8 cut(s) 407, 419, 551, 618, 627, 710, 926, 1130
BshNI GGYRCC 5 cut(s) 72, 359, 611, 769, 826
BsiSI CCGG 7 cut(s) 615, 660, 704, 756, 838, 959, 1345
BslI CCNNNNNNNGG 5 cut(s) 156, 369, 408, 1107, 1238
BsmAI GTCTC 6 cut(s) 15, 214, 290, 338, 377, 824
BsmBI CGTCTC 2 cut(s) 15, 214
BsmI GAATGC 2 cut(s) 107, 973
BsnI GGCC 8 cut(s) 407, 419, 551, 618, 627, 710, 926, 1130
Bso31I GGTCTC 2 cut(s) 338, 377
Bsp119I TTCGAA 1 cut(s) 800
Bsp1286I GDGCHC 2 cut(s) 614, 699
Bsp143I GATC 4 cut(s) 153, 162, 204, 1339
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 8 cut(s) 407, 419, 551, 618, 627, 710, 926, 1130
BspCNI CTCAG 3 cut(s) 192, 373, 964
BspLI GGNNCC 5 cut(s) 74, 361, 613, 771, 828
BspMAI CTGCAG 1 cut(s) 739
BspMI ACCTGC 1 cut(s) 778
BspOI GCTAGC 1 cut(s) 575
BspQI GCTCTTC 1 cut(s) 983
BspT104I TTCGAA 1 cut(s) 800
BspT107I GGYRCC 5 cut(s) 72, 359, 611, 769, 826
BspTNI GGTCTC 2 cut(s) 338, 377
BsrBI CCGCTC 2 cut(s) 114, 350
BsrDI GCAATG 1 cut(s) 1017
BsrFI RCCGGY 1 cut(s) 614
BssAI RCCGGY 1 cut(s) 614
BssMI GATC 4 cut(s) 153, 162, 204, 1339
BssNI GRCGYC 6 cut(s) 5, 73, 234, 360, 864, 873
BssT1I CCWWGG 2 cut(s) 68, 927
Bst2UI CCWGG 3 cut(s) 214, 409, 1233
Bst4CI ACNGT 4 cut(s) 142, 766, 860, 1276
Bst6I CTCTTC 3 cut(s) 103, 384, 983
BstACI GRCGYC 6 cut(s) 5, 73, 234, 360, 864, 873
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 6 cut(s) 174, 507, 573, 639, 675, 973
BstDEI CTNAG 4 cut(s) 179, 381, 564, 951
BstDSI CCRYGG 3 cut(s) 68, 603, 765
BstF5I GGATG 2 cut(s) 440, 1075
BstH2I RGCGCY 2 cut(s) 76, 363
BstHHI GCGC 2 cut(s) 75, 362
BstKTI GATC 4 cut(s) 156, 165, 207, 1342
BstMAI GTCTC 6 cut(s) 15, 214, 290, 338, 377, 824
BstMBI GATC 4 cut(s) 153, 162, 204, 1339
BstNI CCWGG 3 cut(s) 214, 409, 1233
BstNSI RCATGY 1 cut(s) 1198
BstSCI CCNGG 6 cut(s) 212, 407, 754, 837, 957, 1231
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 614
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 706
BstYI RGATCY 1 cut(s) 162
BsuRI GGCC 8 cut(s) 407, 419, 551, 618, 627, 710, 926, 1130
BtgI CCRYGG 3 cut(s) 68, 603, 765
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 2 cut(s) 440, 1075
BtsI GCAGTG 2 cut(s) 102, 531
BtsIMutI CAGTG 4 cut(s) 102, 531, 865, 1309
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 6 cut(s) 174, 507, 573, 639, 675, 973
CfoI GCGC 2 cut(s) 75, 362
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 2 cut(s) 617, 1129
CseI GACGC 3 cut(s) 244, 872, 881
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 6 cut(s) 69, 169, 286, 808, 1195, 1249
DdeI CTNAG 4 cut(s) 179, 381, 564, 951
DinI GGCGCC 2 cut(s) 74, 361
DpnI GATC 4 cut(s) 155, 164, 206, 1341
DpnII GATC 4 cut(s) 153, 162, 204, 1339
EaeI YGGCCR 4 cut(s) 549, 625, 708, 924
Eam1104I CTCTTC 3 cut(s) 103, 384, 983
EarI CTCTTC 3 cut(s) 103, 384, 983
Eco130I CCWWGG 2 cut(s) 68, 927
Eco24I GRGCYC 1 cut(s) 699
Eco31I GGTCTC 2 cut(s) 338, 377
Eco57I CTGAAG 3 cut(s) 1049, 1130, 1285
EcoRII CCWGG 3 cut(s) 212, 407, 1231
EcoT14I CCWWGG 2 cut(s) 68, 927
EcoT38I GRGCYC 1 cut(s) 699
EgeI GGCGCC 2 cut(s) 74, 361
EheI GGCGCC 2 cut(s) 74, 361
ErhI CCWWGG 2 cut(s) 68, 927
Esp3I CGTCTC 2 cut(s) 15, 214
FaeI CATG 6 cut(s) 72, 172, 289, 811, 1198, 1252
FatI CATG 6 cut(s) 68, 168, 285, 807, 1194, 1248
FauI CCCGC 2 cut(s) 493, 750
FbaI TGATCA 1 cut(s) 1339
FokI GGATG 2 cut(s) 427, 1082
FriOI GRGCYC 1 cut(s) 699
FspBI CTAG 1 cut(s) 572
GlaI GCGC 2 cut(s) 74, 361
GsaI CCCAGC 2 cut(s) 449, 505
HaeII RGCGCY 2 cut(s) 76, 363
HaeIII GGCC 8 cut(s) 407, 419, 551, 618, 627, 710, 926, 1130
HapII CCGG 7 cut(s) 615, 660, 704, 756, 838, 959, 1345
HgaI GACGC 3 cut(s) 244, 872, 881
HhaI GCGC 2 cut(s) 75, 362
Hin1I GRCGYC 6 cut(s) 5, 73, 234, 360, 864, 873
Hin1II CATG 6 cut(s) 72, 172, 289, 811, 1198, 1252
Hin6I GCGC 2 cut(s) 73, 360
HinP1I GCGC 2 cut(s) 73, 360
HinfI GANTC 4 cut(s) 269, 313, 1047, 1296
HpaII CCGG 7 cut(s) 615, 660, 704, 756, 838, 959, 1345
HphI GGTGA 5 cut(s) 130, 901, 1168, 1334, 1336
Hpy166II GTNNAC 1 cut(s) 1207
Hpy188I TCNGA 2 cut(s) 226, 274
Hpy188III TCNNGA 2 cut(s) 1067, 1381
Hpy8I GTNNAC 1 cut(s) 1207
HpyAV CCTTC 6 cut(s) 49, 332, 738, 880, 1120, 1384
HpyCH4III ACNGT 4 cut(s) 142, 766, 860, 1276
HpyCH4IV ACGT 3 cut(s) 234, 291, 645
HpyCH4V TGCA 7 cut(s) 107, 494, 737, 787, 917, 978, 1391
HpyF3I CTNAG 4 cut(s) 179, 381, 564, 951
HpySE526I ACGT 3 cut(s) 234, 291, 645
Hsp92I GRCGYC 6 cut(s) 5, 73, 234, 360, 864, 873
Hsp92II CATG 6 cut(s) 72, 172, 289, 811, 1198, 1252
HspAI GCGC 2 cut(s) 73, 360
KasI GGCGCC 2 cut(s) 72, 359
Ksp22I TGATCA 1 cut(s) 1339
Kzo9I GATC 4 cut(s) 153, 162, 204, 1339
LguI GCTCTTC 1 cut(s) 983
LmnI GCTCC 6 cut(s) 119, 188, 355, 514, 721, 1259
LweI GCATC 3 cut(s) 774, 1060, 1207
MaeI CTAG 1 cut(s) 572
MaeII ACGT 3 cut(s) 234, 291, 645
MaeIII GTNAC 4 cut(s) 451, 860, 904, 1330
MalI GATC 4 cut(s) 155, 164, 206, 1341
MbiI CCGCTC 2 cut(s) 114, 350
MboI GATC 4 cut(s) 153, 162, 204, 1339
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 2 cut(s) 614, 699
MlsI TGGCCA 2 cut(s) 551, 926
MluCI AATT 5 cut(s) 495, 594, 630, 663, 1186
MluNI TGGCCA 2 cut(s) 551, 926
Mly113I GGCGCC 2 cut(s) 73, 360
MlyI GAGTC 2 cut(s) 278, 1041
MmeI TCCRAC 2 cut(s) 1062, 1237
Mox20I TGGCCA 2 cut(s) 551, 926
MscI TGGCCA 2 cut(s) 551, 926
MseI TTAA 1 cut(s) 855
MslI CAYNNNNRTG 1 cut(s) 704
Msp20I TGGCCA 2 cut(s) 551, 926
MspA1I CMGCKG 2 cut(s) 449, 713
MspI CCGG 7 cut(s) 615, 660, 704, 756, 838, 959, 1345
MspR9I CCNGG 6 cut(s) 214, 409, 756, 839, 959, 1233
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 2 cut(s) 107, 973
MvaI CCWGG 3 cut(s) 214, 409, 1233
NarI GGCGCC 2 cut(s) 73, 360
NciI CCSGG 3 cut(s) 756, 839, 959
NcoI CCATGG 1 cut(s) 68
NdeII GATC 4 cut(s) 153, 162, 204, 1339
NheI GCTAGC 1 cut(s) 571
NlaIII CATG 6 cut(s) 72, 172, 289, 811, 1198, 1252
NlaIV GGNNCC 5 cut(s) 74, 361, 613, 771, 828
NmeAIII GCCGAG 2 cut(s) 427, 718
NmuCI GTSAC 2 cut(s) 860, 904
NspI RCATGY 1 cut(s) 1198
NspV TTCGAA 1 cut(s) 800
OliI CACNNNNGTG 1 cut(s) 704
PciSI GCTCTTC 1 cut(s) 983
PcsI WCGNNNNNNNCGW 4 cut(s) 264, 324, 339, 384
PctI GAATGC 2 cut(s) 107, 973
PfeI GAWTC 2 cut(s) 313, 1296
PleI GAGTC 2 cut(s) 277, 1041
PluTI GGCGCC 2 cut(s) 76, 363
PpsI GAGTC 2 cut(s) 277, 1041
Psp6I CCWGG 3 cut(s) 212, 407, 1231
PspFI CCCAGC 2 cut(s) 445, 501
PspGI CCWGG 3 cut(s) 212, 407, 1231
PspN4I GGNNCC 5 cut(s) 74, 361, 613, 771, 828
PspPI GGNCC 2 cut(s) 617, 1129
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 162
PvuII CAGCTG 1 cut(s) 449
RseI CAYNNNNRTG 1 cut(s) 704
SapI GCTCTTC 1 cut(s) 983
SaqAI TTAA 1 cut(s) 855
Sau3AI GATC 4 cut(s) 153, 162, 204, 1339
Sau96I GGNCC 2 cut(s) 617, 1129
SchI GAGTC 2 cut(s) 278, 1041
ScrFI CCNGG 6 cut(s) 214, 409, 756, 839, 959, 1233
SduI GDGCHC 2 cut(s) 614, 699
SfaNI GCATC 3 cut(s) 774, 1060, 1207
SfcI CTRYAG 1 cut(s) 735
SfiI GGCCNNNNNGGCC 1 cut(s) 624
SfoI GGCGCC 2 cut(s) 74, 361
SfuI TTCGAA 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 1 cut(s) 1379
SmoI CTYRAG 1 cut(s) 1379
Sse9I AATT 5 cut(s) 495, 594, 630, 663, 1186
SspDI GGCGCC 2 cut(s) 72, 359
SspMI CTAG 1 cut(s) 572
StyD4I CCNGG 6 cut(s) 212, 407, 754, 837, 957, 1231
StyI CCWWGG 2 cut(s) 68, 927
TaaI ACNGT 4 cut(s) 142, 766, 860, 1276
TaiI ACGT 3 cut(s) 237, 294, 648
TaqI TCGA 5 cut(s) 267, 591, 800, 813, 870
TaqII GACCGA 2 cut(s) 209, 401
TasI AATT 5 cut(s) 495, 594, 630, 663, 1186
TauI GCSGC 7 cut(s) 129, 132, 323, 365, 659, 692, 713
TfiI GAWTC 2 cut(s) 313, 1296
Tru1I TTAA 1 cut(s) 855
Tru9I TTAA 1 cut(s) 855
TscAI CASTG 4 cut(s) 109, 538, 865, 1309
TseFI GTSAC 2 cut(s) 860, 904
Tsp45I GTSAC 2 cut(s) 860, 904
TspDTI ATGAA 2 cut(s) 157, 429
TspGWI ACGGA 1 cut(s) 592
TspRI CASTG 4 cut(s) 109, 538, 865, 1309
XceI RCATGY 1 cut(s) 1198
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XspI CTAG 1 cut(s) 572
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.