MD16G1044200.v1.1

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011790
Physical Location & Seq
Reverse (-)
3134142 .. 3137265
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1044200.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGCGTCGTCTCCTTTCCTCTCCAAGACCACCATTTCCTTCTCCTCCTCCATCTCCACCTCCCTCCCATGGCGCCCCTCCCCCTTCTCCTCCTCCTCCACCGCGTTCCGCTCTAATCCCCGCCGCCGCACCCTCACCGTCCAATCCAAGATCAGGGAGATCTTCATGCCCGCACTCAGCTCCACCATGACCGAGGGCAAGATCGTCTCCTGGATCAAATCCGAGGGCGACGTCCTCTCCAAAGGCGAGAGCGTCGTTGTCGTCGAGTCCGACAAAGCCGACATGGACGTCGAGACCTTCTACGACGGCATTCTCGCCGCAATCGTCGTTGGGGAAGGCGAGACCGCCCCCGTCGGCGCCGCCATCGGGCTTCTTGCTGAGACCGAAGAGGAAGTAGCCGAAGCCAGGGAAAAAGCCAAATCCTCCTCGTCTGGTTCCCCCGCTTCTTCCTCCCCAGTTGAAGCTGCTGTTACACCCACTCCTCCTCCTGCTACTGCCACTCCCGCCCCTGCCATTGCCCAGCCTGCTCCATCTGCAACTGCTACTTACACTCCCAAAAAGGGCGTGGCCACTCCTTTCGCAAAAAAGCTAGCCAAGCAGCACAAGGTCGACATTGCCTCCGTGGTGGGCACCGGCCCATTTGGCCGCATTACGCCTGCTGACGTGGAGGCAGCCGCCGGAATTGTCAAGCCCACGAAAGCCGTCGCCGCCGAGCCCACTTCGGTGGCCGCTGCTCCACCCAAGCCTTCTGCTGCAGCCCCGCCACTGCTTCCCGGGTCAACGGTGGTGCCATTTACCACGATGCAGGCTGCCGTCTCGAAGAACATGGTGGAAAGCCTCTCTGTGCCGACTTTCCGGGTCGGGTACCCGATTAACAGCGATGCCATCGACGCCCTCTATGAAAAGGTGAAACCAAAGGGTGTGACAATGACTGCATTGTTGGCCAAGGCTGCTGCTATGGCAGTTGCTCAGCACCCAGTTGTGAATGCGAGCTGCAAAGATGGAAAGAGCTTTTCGTATAACAGCAGTATAAACATTGCTGTTGCTGTGGCAATCAATGGAGGACTCATAACCCCTGTTCTTCAGGATGCCGATAAGTTGGATTTGTATTTGCTGTCCCAGAAATGGAAGGAGTTAGTGGAGAAGGCCCGTTCCAAGCAACTTCAGCCCCATGAGTACAGTACAGGAACTTTCACCTTGTCCAATTTGGGCATGTTTGGAGTGGACAAGTTTGATGCTATTCTTCCACCAGGCCAGGGGGCTATCATGGCTGTTGGAGCTTCAAAGCCAACTGTTGTGGCCGATGTAGATGGATTCTTTAGTGTAAAAAGTAAAATGCTGGTGAACGTAACAGCTGATCACCGGATTGTTTATGGTGCTGATTTGGCTGCCTTCCTCAAGACATTTGCCAACATTGTTGAGAACCCAGAGAGCCTCACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

482

Amino Acids

49.96

Weight (kDa)

8.33

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 53 - 122 3.6e-14 Biotin-requiring enzyme
E3_binding PF02817 190 - 223 4e-13 e3 binding domain
2-oxoacid_dh PF00198 260 - 480 3.2e-63 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25860
fragaria_vesca FvH4_4g27580
malus_domestica MD13G1043400.v1.1 MD16G1044200.v1.1
prunus_persica Prupe.1G309100_v2.0.a1
pyrus_communis pycom13g03820 pycom16g03840
rosa_chinensis RchiOBHm_Chr4g0435441
rosa_laevigata RLG00000006592
rosa_multiflora Rmu_sc0008521.1_g000001
rosa_roxburghii Rroxscaffold_5G00376680
rosa_rugosa Rorug04G0285000 Rorug04G0285100 Rorug05G0266800
rosa_samantha Rh4AG340800 Rh4BG349100 Rh4CG363600 Rh4DG343300
rosa_wichuraiana Rw4G029720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AatII GACGTC 2 cut(s) 234, 291
Acc65I GGTACC 1 cut(s) 864
AccB1I GGYRCC 5 cut(s) 72, 356, 629, 787, 864
AccBSI CCGCTC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 609
AccII CGCG 1 cut(s) 104
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 5 cut(s) 567, 643, 726, 942, 1299
AcuI CTGAAG 2 cut(s) 1067, 1148
AcyI GRCGYC 6 cut(s) 5, 73, 231, 288, 357, 891
AfaI GTAC 3 cut(s) 866, 1178, 1183
AfiI CCNNNNNNNGG 7 cut(s) 69, 153, 366, 405, 560, 1125, 1256
AgsI TTSAA 2 cut(s) 461, 1284
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 4 cut(s) 209, 404, 1249, 1254
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 7 cut(s) 180, 464, 589, 993, 1011, 1280, 1355
AluI AGCT 7 cut(s) 180, 464, 589, 993, 1011, 1280, 1355
Alw26I GTCTC 6 cut(s) 15, 211, 287, 335, 374, 820
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 773
AoxI GGCC 8 cut(s) 567, 634, 643, 726, 942, 1146, 1252, 1299
Asp718I GGTACC 1 cut(s) 864
AspLEI GCGC 2 cut(s) 75, 359
AspS9I GGNCC 2 cut(s) 635, 1147
AsuC2I CCSGG 3 cut(s) 774, 775, 857
AsuHPI GGTGA 5 cut(s) 127, 919, 1186, 1352, 1354
AsuNHI GCTAGC 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 773
BaeGI GKGCMC 1 cut(s) 632
BalI TGGCCA 2 cut(s) 569, 944
BanI GGYRCC 5 cut(s) 72, 356, 629, 787, 864
BanII GRGCYC 1 cut(s) 717
BccI CCATC 6 cut(s) 59, 371, 538, 893, 995, 1304
BceAI ACGGC 3 cut(s) 322, 686, 797
BciT130I CCWGG 4 cut(s) 211, 406, 1251, 1256
BclI TGATCA 1 cut(s) 1357
BcnI CCSGG 3 cut(s) 774, 775, 857
BcoDI GTCTC 6 cut(s) 15, 211, 287, 335, 374, 820
BfaI CTAG 1 cut(s) 590
BfmI CTRYAG 1 cut(s) 753
BfoI RGCGCY 2 cut(s) 76, 360
BglI GCCNNNNNGGC 1 cut(s) 642
BglII AGATCT 1 cut(s) 159
BlpI GCTNAGC 1 cut(s) 969
Bme1390I CCNGG 7 cut(s) 211, 406, 774, 775, 857, 1251, 1256
BmeT110I CYCGRG 1 cut(s) 773
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 2 cut(s) 635, 1147
BmiI GGNNCC 6 cut(s) 74, 358, 436, 631, 789, 866
BmrFI CCNGG 7 cut(s) 211, 406, 774, 775, 857, 1251, 1256
BmrI ACTGGG 2 cut(s) 449, 971
BmsI GCATC 4 cut(s) 792, 871, 1078, 1225
BmtI GCTAGC 1 cut(s) 593
BmuI ACTGGG 2 cut(s) 449, 971
Bpu1102I GCTNAGC 1 cut(s) 969
BpuEI CTTGAG 1 cut(s) 1382
BpuMI CCSGG 3 cut(s) 774, 775, 857
BsaHI GRCGYC 6 cut(s) 5, 73, 231, 288, 357, 891
BsaI GGTCTC 3 cut(s) 287, 335, 374
BsaJI CCNNGG 8 cut(s) 68, 192, 222, 405, 621, 773, 945, 1255
BsaWI WCCGGW 1 cut(s) 1362
Bsc4I CCNNNNNNNGG 7 cut(s) 69, 153, 366, 405, 560, 1125, 1256
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 2 cut(s) 455, 977
Bse3DI GCAATG 3 cut(s) 513, 612, 1035
BseBI CCWGG 4 cut(s) 211, 406, 1251, 1256
BseDI CCNNGG 8 cut(s) 68, 192, 222, 405, 621, 773, 945, 1255
BseGI GGATG 1 cut(s) 1093
BseLI CCNNNNNNNGG 7 cut(s) 69, 153, 366, 405, 560, 1125, 1256
BseMI GCAATG 3 cut(s) 513, 612, 1035
BseMII CTCAG 3 cut(s) 190, 369, 983
BseNI ACTGG 2 cut(s) 455, 977
BseRI GAGGAG 8 cut(s) 34, 37, 79, 82, 85, 415, 471, 474
BseSI GKGCMC 1 cut(s) 632
BseYI CCCAGC 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 8 cut(s) 569, 636, 645, 728, 944, 1148, 1254, 1301
BshNI GGYRCC 5 cut(s) 72, 356, 629, 787, 864
BsiHKCI CYCGRG 1 cut(s) 773
BsiSI CCGG 5 cut(s) 633, 678, 774, 856, 1363
BslFI GGGAC 1 cut(s) 1102
BslI CCNNNNNNNGG 7 cut(s) 69, 153, 366, 405, 560, 1125, 1256
BsmAI GTCTC 6 cut(s) 15, 211, 287, 335, 374, 820
BsmBI CGTCTC 3 cut(s) 15, 211, 820
BsmFI GGGAC 1 cut(s) 1102
BsmI GAATGC 2 cut(s) 309, 991
BsnI GGCC 8 cut(s) 569, 636, 645, 728, 944, 1148, 1254, 1301
Bso31I GGTCTC 3 cut(s) 287, 335, 374
BsoBI CYCGRG 1 cut(s) 773
Bsp1286I GDGCHC 2 cut(s) 632, 717
Bsp143I GATC 5 cut(s) 150, 159, 201, 213, 1357
Bsp1720I GCTNAGC 1 cut(s) 969
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 8 cut(s) 569, 636, 645, 728, 944, 1148, 1254, 1301
BspCNI CTCAG 3 cut(s) 189, 370, 982
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 6 cut(s) 74, 358, 436, 631, 789, 866
BspMAI CTGCAG 1 cut(s) 757
BspOI GCTAGC 1 cut(s) 593
BspPI GGATC 1 cut(s) 221
BspT107I GGYRCC 5 cut(s) 72, 356, 629, 787, 864
BspTNI GGTCTC 3 cut(s) 287, 335, 374
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 3 cut(s) 513, 612, 1035
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 2 cut(s) 455, 977
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 8 cut(s) 68, 192, 222, 405, 621, 773, 945, 1255
BssMI GATC 5 cut(s) 150, 159, 201, 213, 1357
BssNI GRCGYC 6 cut(s) 5, 73, 231, 288, 357, 891
BssT1I CCWWGG 2 cut(s) 68, 945
Bst2UI CCWGG 4 cut(s) 211, 406, 1251, 1256
Bst4CI ACNGT 4 cut(s) 139, 784, 1181, 1294
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 6 cut(s) 5, 73, 231, 288, 357, 891
BstC8I GCNNGC 6 cut(s) 171, 525, 591, 657, 807, 991
BstDEI CTNAG 3 cut(s) 176, 378, 969
BstDSI CCRYGG 2 cut(s) 68, 621
BstF5I GGATG 1 cut(s) 1093
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 2 cut(s) 76, 360
BstHHI GCGC 2 cut(s) 75, 359
BstKTI GATC 5 cut(s) 153, 162, 204, 216, 1360
BstMAI GTCTC 6 cut(s) 15, 211, 287, 335, 374, 820
BstMBI GATC 5 cut(s) 150, 159, 201, 213, 1357
BstNI CCWGG 4 cut(s) 211, 406, 1251, 1256
BstNSI RCATGY 1 cut(s) 1216
BstSCI CCNGG 7 cut(s) 209, 404, 772, 773, 855, 1249, 1254
BstSFI CTRYAG 1 cut(s) 753
BstSLI GKGCMC 1 cut(s) 632
BstUI CGCG 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 159
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 8 cut(s) 569, 636, 645, 728, 944, 1148, 1254, 1301
BtgI CCRYGG 2 cut(s) 68, 621
BtgZI GCGATG 1 cut(s) 894
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 1 cut(s) 1093
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 1 cut(s) 764
Cac8I GCNNGC 6 cut(s) 171, 525, 591, 657, 807, 991
CfoI GCGC 2 cut(s) 75, 359
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 635, 1147
Cfr9I CCCGGG 1 cut(s) 773
CseI GACGC 2 cut(s) 241, 899
Csp6I GTAC 3 cut(s) 865, 1177, 1182
CspCI CAANNNNNGTGG 2 cut(s) 1278, 1313
CviAII CATG 8 cut(s) 69, 166, 187, 283, 826, 1172, 1213, 1267
CviQI GTAC 3 cut(s) 865, 1177, 1182
DdeI CTNAG 3 cut(s) 176, 378, 969
DinI GGCGCC 2 cut(s) 74, 358
DpnI GATC 5 cut(s) 152, 161, 203, 215, 1359
DpnII GATC 5 cut(s) 150, 159, 201, 213, 1357
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 5 cut(s) 567, 643, 726, 942, 1299
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 68, 945
Eco24I GRGCYC 1 cut(s) 717
Eco31I GGTCTC 3 cut(s) 287, 335, 374
Eco57I CTGAAG 2 cut(s) 1067, 1148
Eco88I CYCGRG 1 cut(s) 773
EcoRII CCWGG 4 cut(s) 209, 404, 1249, 1254
EcoT14I CCWWGG 2 cut(s) 68, 945
EcoT38I GRGCYC 1 cut(s) 717
EgeI GGCGCC 2 cut(s) 74, 358
EheI GGCGCC 2 cut(s) 74, 358
ErhI CCWWGG 2 cut(s) 68, 945
Esp3I CGTCTC 3 cut(s) 15, 211, 820
FaeI CATG 8 cut(s) 72, 169, 190, 286, 829, 1175, 1216, 1270
FaqI GGGAC 1 cut(s) 1102
FatI CATG 8 cut(s) 68, 165, 186, 282, 825, 1171, 1212, 1266
FauI CCCGC 5 cut(s) 128, 178, 448, 511, 768
FbaI TGATCA 1 cut(s) 1357
FblI GTMKAC 1 cut(s) 609
FokI GGATG 1 cut(s) 1100
FriOI GRGCYC 1 cut(s) 717
FspBI CTAG 1 cut(s) 590
GlaI GCGC 2 cut(s) 74, 358
GsaI CCCAGC 1 cut(s) 523
HaeII RGCGCY 2 cut(s) 76, 360
HaeIII GGCC 8 cut(s) 569, 636, 645, 728, 944, 1148, 1254, 1301
HapII CCGG 5 cut(s) 633, 678, 774, 856, 1363
HgaI GACGC 2 cut(s) 241, 899
HhaI GCGC 2 cut(s) 75, 359
Hin1I GRCGYC 6 cut(s) 5, 73, 231, 288, 357, 891
Hin1II CATG 8 cut(s) 72, 169, 190, 286, 829, 1175, 1216, 1270
Hin6I GCGC 2 cut(s) 73, 357
HinP1I GCGC 2 cut(s) 73, 357
HincII GTYRAC 2 cut(s) 610, 780
HindII GTYRAC 2 cut(s) 610, 780
HinfI GANTC 3 cut(s) 266, 1065, 1314
HpaII CCGG 5 cut(s) 633, 678, 774, 856, 1363
HphI GGTGA 5 cut(s) 127, 919, 1186, 1352, 1354
Hpy166II GTNNAC 4 cut(s) 610, 780, 1225, 1345
Hpy188I TCNGA 2 cut(s) 223, 271
Hpy188III TCNNGA 4 cut(s) 292, 817, 1085, 1399
Hpy8I GTNNAC 4 cut(s) 610, 780, 1225, 1345
HpyAV CCTTC 8 cut(s) 49, 94, 307, 329, 756, 1123, 1138, 1402
HpyCH4III ACNGT 4 cut(s) 139, 784, 1181, 1294
HpyCH4IV ACGT 4 cut(s) 231, 288, 663, 1347
HpyCH4V TGCA 5 cut(s) 536, 755, 805, 935, 996
HpyF3I CTNAG 3 cut(s) 176, 378, 969
HpySE526I ACGT 4 cut(s) 231, 288, 663, 1347
Hsp92I GRCGYC 6 cut(s) 5, 73, 231, 288, 357, 891
Hsp92II CATG 8 cut(s) 72, 169, 190, 286, 829, 1175, 1216, 1270
HspAI GCGC 2 cut(s) 73, 357
KasI GGCGCC 2 cut(s) 72, 356
KpnI GGTACC 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 1357
Kzo9I GATC 5 cut(s) 150, 159, 201, 213, 1357
LmnI GCTCC 4 cut(s) 185, 532, 739, 1277
LweI GCATC 4 cut(s) 792, 871, 1078, 1225
MaeI CTAG 1 cut(s) 590
MaeII ACGT 4 cut(s) 231, 288, 663, 1347
MaeIII GTNAC 3 cut(s) 469, 922, 1348
MalI GATC 5 cut(s) 152, 161, 203, 215, 1359
MbiI CCGCTC 1 cut(s) 111
MboI GATC 5 cut(s) 150, 159, 201, 213, 1357
MboII GAAGA 6 cut(s) 154, 398, 438, 832, 1073, 1235
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 632, 717
MlsI TGGCCA 2 cut(s) 569, 944
MluCI AATT 2 cut(s) 681, 1204
MluNI TGGCCA 2 cut(s) 569, 944
Mly113I GGCGCC 2 cut(s) 73, 357
MlyI GAGTC 2 cut(s) 275, 1059
MmeI TCCRAC 3 cut(s) 294, 1080, 1255
Mox20I TGGCCA 2 cut(s) 569, 944
MscI TGGCCA 2 cut(s) 569, 944
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 1 cut(s) 722
Msp20I TGGCCA 2 cut(s) 569, 944
MspA1I CMGCKG 2 cut(s) 731, 1355
MspI CCGG 5 cut(s) 633, 678, 774, 856, 1363
MspR9I CCNGG 7 cut(s) 211, 406, 774, 775, 857, 1251, 1256
Mva1269I GAATGC 2 cut(s) 309, 991
MvaI CCWGG 4 cut(s) 211, 406, 1251, 1256
MvnI CGCG 1 cut(s) 104
NarI GGCGCC 2 cut(s) 73, 357
NciI CCSGG 3 cut(s) 774, 775, 857
NcoI CCATGG 1 cut(s) 68
NdeII GATC 5 cut(s) 150, 159, 201, 213, 1357
NheI GCTAGC 1 cut(s) 589
NlaIII CATG 8 cut(s) 72, 169, 190, 286, 829, 1175, 1216, 1270
NlaIV GGNNCC 6 cut(s) 74, 358, 436, 631, 789, 866
NmeAIII GCCGAG 1 cut(s) 736
NmuCI GTSAC 1 cut(s) 922
NspI RCATGY 1 cut(s) 1216
OliI CACNNNNGTG 1 cut(s) 722
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 267, 321
PctI GAATGC 2 cut(s) 309, 991
PfeI GAWTC 1 cut(s) 1314
PfoI TCCNGGA 1 cut(s) 209
PleI GAGTC 2 cut(s) 274, 1059
PluTI GGCGCC 2 cut(s) 76, 360
PpsI GAGTC 2 cut(s) 274, 1059
Psp6I CCWGG 4 cut(s) 209, 404, 1249, 1254
PspFI CCCAGC 1 cut(s) 519
PspGI CCWGG 4 cut(s) 209, 404, 1249, 1254
PspN4I GGNNCC 6 cut(s) 74, 358, 436, 631, 789, 866
PspPI GGNCC 2 cut(s) 635, 1147
PstI CTGCAG 1 cut(s) 757
PsuI RGATCY 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 1355
RsaI GTAC 3 cut(s) 866, 1178, 1183
RsaNI GTAC 3 cut(s) 865, 1177, 1182
RseI CAYNNNNRTG 1 cut(s) 722
SalI GTCGAC 1 cut(s) 608
SaqAI TTAA 1 cut(s) 873
Sau3AI GATC 5 cut(s) 150, 159, 201, 213, 1357
Sau96I GGNCC 2 cut(s) 635, 1147
SchI GAGTC 2 cut(s) 275, 1059
ScrFI CCNGG 7 cut(s) 211, 406, 774, 775, 857, 1251, 1256
SduI GDGCHC 2 cut(s) 632, 717
SfaNI GCATC 4 cut(s) 792, 871, 1078, 1225
SfcI CTRYAG 1 cut(s) 753
SfiI GGCCNNNNNGGCC 1 cut(s) 642
SfoI GGCGCC 2 cut(s) 74, 358
SmaI CCCGGG 1 cut(s) 775
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 1397
SmoI CTYRAG 1 cut(s) 1397
Sse9I AATT 2 cut(s) 681, 1204
SspDI GGCGCC 2 cut(s) 72, 356
SspMI CTAG 1 cut(s) 590
StyD4I CCNGG 7 cut(s) 209, 404, 772, 773, 855, 1249, 1254
StyI CCWWGG 2 cut(s) 68, 945
TaaI ACNGT 4 cut(s) 139, 784, 1181, 1294
TaiI ACGT 4 cut(s) 234, 291, 666, 1350
TaqI TCGA 5 cut(s) 264, 291, 609, 818, 888
TaqII GACCGA 2 cut(s) 206, 398
TasI AATT 2 cut(s) 681, 1204
TatI WGTACW 2 cut(s) 1176, 1181
TauI GCSGC 8 cut(s) 126, 129, 320, 362, 648, 677, 710, 731
TfiI GAWTC 1 cut(s) 1314
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 1 cut(s) 771
TseFI GTSAC 1 cut(s) 922
Tsp45I GTSAC 1 cut(s) 922
TspDTI ATGAA 2 cut(s) 154, 915
TspGWI ACGGA 1 cut(s) 610
TspMI CCCGGG 1 cut(s) 773
TspRI CASTG 1 cut(s) 771
XceI RCATGY 1 cut(s) 1216
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmaI CCCGGG 1 cut(s) 773
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 1 cut(s) 590
ZraI GACGTC 2 cut(s) 232, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.