FvH4_4g29150

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007725
Physical Location & Seq
Forward (+)
29416015 .. 29418611
2597 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29150.t1

Sequence Viewer

Length: 690 bp
ATGTCGAAAGCAAATGTAAGAAGACAATTAGTTCTTCTGGAGAAGAACAAGGTTCCCAAAGAGAAGAGTACTACAACTACTGCATTCAGCCCCATATTCTCTTATAAACACCTAAAGAGAATCTACCCAATTGGGCTGCACAGGAGTTCTTCATCATCTTCCTCTCTATCTTTATCATCCTTATCTTTGTCTTTGTCAGAAAACTCCATTGACTCTTCTTCTATCATTGATTCTGCTTCTCCACTCGAGCAGAAGATATCATTAGCGCTCCGCCTCATTGCACCACCGCAAAGAAGAGAGTCTCCGGTGCCTAAAGTTGTTCAACAGCAAAGTCAGACCTTTCAGGATACTGATAATGGGGAGTTGAGGAGGTGCAATTGGATAACAAAGAATAGTGATAAAGTTTATGTTGCATTTCATGATGAATGCTGGGGAGTTCCAGTATATGATGACAATCAATTATTTGAGCTGCTTGCACTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTAAAAAGAAGGGAGCTATTCAGGGAAGCCTTTTCTGGATTTGATCCAAACATTGTTGCTAAAATGGGGGAGGAAGAGATTGAAGAGATAGCTTCCAACAAAGCTTTGATGTTGCCGGACTGCAAAGTCAGGTGCATTGTAGAAAATGCCAAATGCATATTGAAGGCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

230

Amino Acids

25.85

Weight (kDa)

8.23

Isoelectric Point (pI)

72.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 130 - 227 2.1e-31 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
AasI GACNNNNNNGTC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 307
AciI CCGC 2 cut(s) 271, 287
AclWI GGATC 1 cut(s) 554
AfaI GTAC 1 cut(s) 70
AfeI AGCGCT 1 cut(s) 267
AgsI TTSAA 3 cut(s) 323, 599, 679
AluBI AGCT 5 cut(s) 469, 532, 608, 620, 687
AluI AGCT 5 cut(s) 469, 532, 608, 620, 687
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 245
Aor51HI AGCGCT 1 cut(s) 267
ApeKI GCWGC 2 cut(s) 136, 469
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 28
BbvI GCAGC 2 cut(s) 123, 456
BccI CCATC 1 cut(s) 487
BciVI GTATCC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 1 cut(s) 688
BfoI RGCGCY 1 cut(s) 269
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 2 cut(s) 137, 470
BlsI GCNGC 2 cut(s) 138, 471
BmcAI AGTACT 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 496
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 2 cut(s) 54, 309
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 59
BsaBI GATNNNNATC 1 cut(s) 453
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 453
BseGI GGATG 1 cut(s) 176
BseJI GATNNNNATC 1 cut(s) 453
BseMI GCAATG 1 cut(s) 276
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 382
BseXI GCAGC 2 cut(s) 123, 456
BseYI CCCAGC 1 cut(s) 429
BsgI GTGCAG 1 cut(s) 122
BshNI GGYRCC 1 cut(s) 307
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 305, 632
BsmAI GTCTC 1 cut(s) 306
BsmI GAATGC 2 cut(s) 83, 431
BsoBI CYCGRG 1 cut(s) 245
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 2 cut(s) 271, 287
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 2 cut(s) 54, 309
BspPI GGATC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 1 cut(s) 440
BssMI GATC 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 5 cut(s) 59, 220, 289, 585, 594
BstC8I GCNNGC 2 cut(s) 474, 685
BstF5I GGATG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 1 cut(s) 562
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 1 cut(s) 559
BstV1I GCAGC 2 cut(s) 123, 456
BstV2I GAAGAC 1 cut(s) 28
BsuI GTATCC 1 cut(s) 340
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 476
Cac8I GCNNGC 2 cut(s) 474, 685
CciI TCATGA 1 cut(s) 418
CfoI GCGC 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 496
Csp6I GTAC 1 cut(s) 69
CviAII CATG 1 cut(s) 419
CviJI RGCY 8 cut(s) 90, 136, 469, 532, 545, 608, 620, 687
CviKI_1 RGCY 8 cut(s) 90, 136, 469, 532, 545, 608, 620, 687
CviQI GTAC 1 cut(s) 69
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
Eam1104I CTCTTC 5 cut(s) 59, 220, 289, 585, 594
EarI CTCTTC 5 cut(s) 59, 220, 289, 585, 594
EciI GGCGGA 1 cut(s) 260
Eco32I GATATC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 496
Eco47III AGCGCT 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 245
EcoRV GATATC 1 cut(s) 258
EcoT22I ATGCAT 1 cut(s) 674
FaeI CATG 1 cut(s) 422
FaiI YATR 8 cut(s) 95, 105, 408, 420, 445, 447, 488, 674
FatI CATG 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 137, 470
FokI GGATG 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 137, 470
FspBI CTAG 1 cut(s) 688
GlaI GCGC 1 cut(s) 267
GluI GCNGC 2 cut(s) 137, 470
GsaI CCCAGC 1 cut(s) 433
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 269
HapII CCGG 2 cut(s) 305, 632
HhaI GCGC 1 cut(s) 268
Hin1II CATG 1 cut(s) 422
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HindIII AAGCTT 1 cut(s) 618
HinfI GANTC 4 cut(s) 120, 212, 230, 299
HpaII CCGG 2 cut(s) 305, 632
Hpy188I TCNGA 2 cut(s) 199, 336
Hpy188III TCNNGA 4 cut(s) 38, 344, 419, 552
HpyAV CCTTC 2 cut(s) 519, 673
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4V TGCA 9 cut(s) 83, 139, 281, 375, 413, 476, 639, 651, 672
Hsp92II CATG 1 cut(s) 422
HspAI GCGC 1 cut(s) 266
Kzo9I GATC 1 cut(s) 559
LmnI GCTCC 2 cut(s) 273, 529
Lsp1109I GCAGC 2 cut(s) 123, 456
MaeI CTAG 1 cut(s) 688
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MfeI CAATTG 3 cut(s) 129, 376, 502
MluCI AATT 6 cut(s) 26, 129, 376, 458, 502, 513
MlyI GAGTC 2 cut(s) 206, 308
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 5 cut(s) 172, 284, 360, 363, 580
Mph1103I ATGCAT 1 cut(s) 674
MspI CCGG 2 cut(s) 305, 632
MunI CAATTG 3 cut(s) 129, 376, 502
Mva1269I GAATGC 2 cut(s) 83, 431
NdeII GATC 1 cut(s) 559
NlaIII CATG 1 cut(s) 422
NlaIV GGNNCC 2 cut(s) 54, 309
NsiI ATGCAT 1 cut(s) 674
PaeR7I CTCGAG 1 cut(s) 245
PagI TCATGA 1 cut(s) 418
PctI GAATGC 2 cut(s) 83, 431
PfeI GAWTC 2 cut(s) 120, 230
PkrI GCNGC 2 cut(s) 138, 471
PleI GAGTC 2 cut(s) 206, 307
PpsI GAGTC 2 cut(s) 206, 307
PsiI TTATAA 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 54, 309
PspPI GGNCC 1 cut(s) 496
PspXI VCTCGAGB 1 cut(s) 245
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
SatI GCNGC 2 cut(s) 137, 470
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 1 cut(s) 496
ScaI AGTACT 1 cut(s) 70
SchI GAGTC 2 cut(s) 206, 308
Sfr274I CTCGAG 1 cut(s) 245
SinI GGWCC 1 cut(s) 496
SlaI CTCGAG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 6 cut(s) 26, 129, 376, 458, 502, 513
SsiI CCGC 2 cut(s) 271, 287
SspMI CTAG 1 cut(s) 688
TaaI ACNGT 1 cut(s) 480
TaqI TCGA 2 cut(s) 5, 246
TasI AATT 6 cut(s) 26, 129, 376, 458, 502, 513
TatI WGTACW 1 cut(s) 68
TfiI GAWTC 2 cut(s) 120, 230
TscAI CASTG 1 cut(s) 483
TseI GCWGC 2 cut(s) 136, 469
TspDTI ATGAA 3 cut(s) 141, 407, 438
TspRI CASTG 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 245
XspI CTAG 1 cut(s) 688
ZrmI AGTACT 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.