MD16G1054800.v1.1

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007725
Physical Location & Seq
Forward (+)
3871263 .. 3874513
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1054800.v1.1.491

Sequence Viewer

Length: 948 bp
ATGTCTAAAGCAAATATTTTAAGAAGACATGTACTTCTGGAGAAGAACAAAGTTGTAAAGGAGAGGGAGAAGACGAACAGTAACCCTAAACACCTCAAACGAATTTACCCAATTGGCCTTCACAAGAGCACTTCATCTCTATCCCTATCTTCATCATCATCTTTGTCATTCTCCTTGTCGGAGAACTCGTATGATTCTTCTCTCACCGATTCTACCTTTCCACTGGATCAGAAGATTTCCGCGGCGTTCCGCTTCATTGCACCACCACCTCCAAGAAGAGAGTATAATTCTCCAGTGGCTAAACTTGCCCAGCAGCAGATTAGTCTGGCTCAGGATGCTAATGATGGGGAGTTGAGGAGGTGCAATTGGATAACAAAGAATAGTGATAAAGTTTATGTAGCGTTTCATGACGAATGCTGGGGAGTTCCAGCATACGATGACAATCAATTGTTTGAGCTGCTTGCACTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTTTTCAGGGAAGCGTTTTCTGGATTCGATCCGAACAAAGTTGCAAAAATGGAGGAGAAAGAGATTGCAGAAATAACTTCCAACAAAGAAATAATGCTGGTAGACAGCAGAGTGAGGTGCATGATAAACAATGCCAAATGCATATTGAAGATTGTGAGGGAGTTTGGATCTTTCAGCAGCTACATTTGGAGTTATGTGAATCACAAACCAGTCATAAACCGATTCAGATACCCCAGAAACGTTCCTCTGAGGACCCCGAAAGCAGAATCCATGAGCAAGGATTTGATCAAGAGAGGGTTTCGATACGTTGGACCGGTGATTGTGTACTCGTTCATGCAGGCTGCAGGGTTGACAAATGATCATCTGGTGGATTGTTATAGGTACAGTGAATGTGTGAACCTTGCAGAAAGACCTTGGAGACATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

316

Amino Acids

36.39

Weight (kDa)

9.38

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 126 - 300 1.3e-68 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 621
AccII CGCG 1 cut(s) 242
AciI CCGC 3 cut(s) 240, 242, 250
AclI AACGTT 1 cut(s) 759
AclWI GGATC 3 cut(s) 234, 542, 694
AcsI RAATTY 1 cut(s) 102
AfaI GTAC 3 cut(s) 33, 845, 902
AflIII ACRYGT 1 cut(s) 28
AgeI ACCGGT 1 cut(s) 832
AgsI TTSAA 1 cut(s) 667
AluBI AGCT 3 cut(s) 457, 520, 699
AluI AGCT 3 cut(s) 457, 520, 699
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 931
AlwI GGATC 3 cut(s) 234, 542, 694
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 313, 457, 696, 860
ApoI RAATTY 1 cut(s) 102
AsiGI ACCGGT 1 cut(s) 832
AspS9I GGNCC 3 cut(s) 484, 771, 830
AsuHPI GGTGA 2 cut(s) 196, 847
AvaII GGWCC 3 cut(s) 484, 771, 830
BbsI GAAGAC 2 cut(s) 31, 77
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 4 cut(s) 325, 444, 708, 847
BccI CCATC 2 cut(s) 338, 475
BclI TGATCA 2 cut(s) 804, 877
BcoDI GTCTC 1 cut(s) 931
BfmI CTRYAG 1 cut(s) 861
BisI GCNGC 5 cut(s) 243, 314, 458, 697, 861
BlsI GCNGC 5 cut(s) 244, 315, 459, 698, 862
Bme18I GGWCC 3 cut(s) 484, 771, 830
BmgT120I GGNCC 3 cut(s) 484, 771, 830
BmiI GGNNCC 1 cut(s) 773
BmsI GCATC 1 cut(s) 325
BpiI GAAGAC 2 cut(s) 31, 77
BpmI CTGGAG 2 cut(s) 59, 276
Bpu10I CCTNAGC 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 2 cut(s) 240, 932
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 3 cut(s) 228, 293, 728
Bse3DI GCAATG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 441
BseDI CCNNGG 2 cut(s) 240, 932
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 1 cut(s) 441
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 344, 758
BseNI ACTGG 3 cut(s) 228, 293, 728
BseRI GAGGAG 2 cut(s) 370, 587
BseXI GCAGC 4 cut(s) 325, 444, 708, 847
BseYI CCCAGC 2 cut(s) 309, 417
Bsh1236I CGCG 1 cut(s) 242
BshFI GGCC 1 cut(s) 117
BshTI ACCGGT 1 cut(s) 832
BsiHKAI GWGCWC 1 cut(s) 131
BsiSI CCGG 1 cut(s) 833
BsmAI GTCTC 1 cut(s) 931
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 5 cut(s) 226, 547, 686, 804, 877
BspACI CCGC 3 cut(s) 240, 242, 250
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 343, 759
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 1 cut(s) 773
BspMAI CTGCAG 1 cut(s) 865
BspPI GGATC 3 cut(s) 234, 542, 694
BsrDI GCAATG 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 832
BsrI ACTGG 3 cut(s) 228, 293, 728
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 2 cut(s) 240, 932
BssMI GATC 5 cut(s) 226, 547, 686, 804, 877
BssT1I CCWWGG 1 cut(s) 932
Bst4CI ACNGT 3 cut(s) 80, 468, 905
Bst6I CTCTTC 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 462, 858
BstDEI CTNAG 2 cut(s) 330, 767
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 5 cut(s) 229, 550, 689, 807, 880
BstMAI GTCTC 1 cut(s) 931
BstMBI GATC 5 cut(s) 226, 547, 686, 804, 877
BstMWI GCNNNNNNNGC 2 cut(s) 305, 335
BstNSI RCATGY 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 861
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 4 cut(s) 325, 444, 708, 847
BstV2I GAAGAC 2 cut(s) 31, 77
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 4 cut(s) 221, 300, 464, 910
Cac8I GCNNGC 2 cut(s) 462, 858
CciI TCATGA 1 cut(s) 406
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 3 cut(s) 484, 771, 830
Cfr42I CCGCGG 1 cut(s) 243
Csp6I GTAC 3 cut(s) 32, 844, 901
CspAI ACCGGT 1 cut(s) 832
CviAII CATG 5 cut(s) 29, 407, 640, 790, 853
CviJI RGCY 7 cut(s) 117, 299, 329, 457, 520, 699, 860
CviKI_1 RGCY 7 cut(s) 117, 299, 329, 457, 520, 699, 860
CviQI GTAC 3 cut(s) 32, 844, 901
DdeI CTNAG 2 cut(s) 330, 767
DpnI GATC 5 cut(s) 228, 549, 688, 806, 879
DpnII GATC 5 cut(s) 226, 547, 686, 804, 877
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 932
Eco47I GGWCC 3 cut(s) 484, 771, 830
EcoO109I RGGNCCY 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 932
EcoT22I ATGCAT 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 932
FaeI CATG 5 cut(s) 32, 410, 643, 793, 856
FatI CATG 5 cut(s) 28, 406, 639, 789, 852
FbaI TGATCA 2 cut(s) 804, 877
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 5 cut(s) 243, 314, 458, 697, 861
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 5 cut(s) 243, 314, 458, 697, 861
GluI GCNGC 5 cut(s) 243, 314, 458, 697, 861
GsaI CCCAGC 2 cut(s) 313, 421
GsuI CTGGAG 2 cut(s) 59, 276
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 833
Hin1II CATG 5 cut(s) 32, 410, 643, 793, 856
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HinfI GANTC 6 cut(s) 194, 209, 543, 718, 741, 785
HpaII CCGG 1 cut(s) 833
HphI GGTGA 2 cut(s) 196, 847
Hpy166II GTNNAC 4 cut(s) 622, 844, 870, 916
Hpy188I TCNGA 5 cut(s) 181, 231, 552, 746, 768
Hpy188III TCNNGA 5 cut(s) 38, 332, 407, 540, 808
Hpy8I GTNNAC 4 cut(s) 622, 844, 870, 916
HpyAV CCTTC 2 cut(s) 128, 507
HpyCH4III ACNGT 3 cut(s) 80, 468, 905
HpyCH4IV ACGT 2 cut(s) 759, 825
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 335
HpyF3I CTNAG 2 cut(s) 330, 767
HpySE526I ACGT 2 cut(s) 759, 825
Hsp92II CATG 5 cut(s) 32, 410, 643, 793, 856
Ksp22I TGATCA 2 cut(s) 804, 877
KspI CCGCGG 1 cut(s) 243
Kzo9I GATC 5 cut(s) 226, 547, 686, 804, 877
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 4 cut(s) 325, 444, 708, 847
LweI GCATC 1 cut(s) 325
MaeII ACGT 2 cut(s) 759, 825
MaeIII GTNAC 1 cut(s) 80
MalI GATC 5 cut(s) 228, 549, 688, 806, 879
MboI GATC 5 cut(s) 226, 547, 686, 804, 877
MboII GAAGA 8 cut(s) 36, 55, 82, 141, 189, 244, 288, 679
MfeI CAATTG 4 cut(s) 111, 364, 446, 490
MflI RGATCY 1 cut(s) 686
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 7 cut(s) 102, 111, 286, 364, 446, 490, 501
MmeI TCCRAC 3 cut(s) 159, 624, 808
Mph1103I ATGCAT 1 cut(s) 662
MseI TTAA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 1 cut(s) 833
MunI CAATTG 4 cut(s) 111, 364, 446, 490
Mva1269I GAATGC 1 cut(s) 419
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 305, 335
NdeII GATC 5 cut(s) 226, 547, 686, 804, 877
NlaIII CATG 5 cut(s) 32, 410, 643, 793, 856
NlaIV GGNNCC 1 cut(s) 773
NsiI ATGCAT 1 cut(s) 662
NspI RCATGY 1 cut(s) 32
PagI TCATGA 1 cut(s) 406
PciI ACATGT 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 6 cut(s) 194, 209, 543, 718, 741, 785
PinAI ACCGGT 1 cut(s) 832
PkrI GCNGC 5 cut(s) 244, 315, 459, 698, 862
PpuMI RGGWCCY 1 cut(s) 771
PscI ACATGT 1 cut(s) 28
Psp1406I AACGTT 1 cut(s) 759
Psp5II RGGWCCY 1 cut(s) 771
PspFI CCCAGC 2 cut(s) 309, 417
PspN4I GGNNCC 1 cut(s) 773
PspPI GGNCC 3 cut(s) 484, 771, 830
PspPPI RGGWCCY 1 cut(s) 771
PstI CTGCAG 1 cut(s) 865
PsuI RGATCY 1 cut(s) 686
RsaI GTAC 3 cut(s) 33, 845, 902
RsaNI GTAC 3 cut(s) 32, 844, 901
SacII CCGCGG 1 cut(s) 243
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 5 cut(s) 243, 314, 458, 697, 861
Sau3AI GATC 5 cut(s) 226, 547, 686, 804, 877
Sau96I GGNCC 3 cut(s) 484, 771, 830
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 861
Sfr303I CCGCGG 1 cut(s) 243
SgrBI CCGCGG 1 cut(s) 243
SinI GGWCC 3 cut(s) 484, 771, 830
Sse9I AATT 7 cut(s) 102, 111, 286, 364, 446, 490, 501
SsiI CCGC 3 cut(s) 240, 242, 250
SspI AATATT 1 cut(s) 16
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 3 cut(s) 80, 468, 905
TaiI ACGT 2 cut(s) 762, 828
TaqI TCGA 2 cut(s) 546, 820
TasI AATT 7 cut(s) 102, 111, 286, 364, 446, 490, 501
TatI WGTACW 2 cut(s) 31, 843
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 6 cut(s) 194, 209, 543, 718, 741, 785
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 4 cut(s) 228, 300, 471, 910
TseI GCWGC 4 cut(s) 313, 457, 696, 860
TspDTI ATGAA 5 cut(s) 123, 141, 244, 395, 841
TspRI CASTG 4 cut(s) 228, 300, 471, 910
VpaK11BI GGWCC 3 cut(s) 484, 771, 830
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 621
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.