Rh4AG353600

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007725
Physical Location & Seq
Forward (+)
66225990 .. 66230126
4137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG353600.1

Sequence Viewer

Length: 1116 bp
ATGCATGAAACTCACACTTCACCAACCAACTTTGAGCATAACCCTTTTCCATACCTCAAATTTAATTTGATTGTCAATACCTTATATAACTCAATCCACTTTAACATATCTATCCCATTTAAGCAGGAGACACAGCTTCATTTCTTTGTCCTCTGCAACATGTCTAAAGCAAATGTAAGAAGACAATTAGTACTTCTGGAGAAGAACAAGGTTCCCAAGGAGAAGAGTACTACTAGTGCTTTCAGCTCCCACTTTTCTTATAAACACCTAAAGAGAATCTACCCAATTGGGCTTCACAAGAGTTCTTCATCTTCCTCTCTATCTCTATCATCCTTATCCTTGTCTTTGTCAGAAAACTCCATTGACTCTTCTTCTATCATTGATTCTACTTCTCCACTCGATCAGAAGATTTCATCAGCACTCCGCCTCATTGCACCGCCGCCCCAAAGAAGAGAGTCTCCGGTGCCTAAAGTTGTCCAACAGCAGAATCAGAGCTTCCAGGATACTGATAATGGGGAGTTGAGGAGGTGCAATTGGATAACAAAGAACAGCGATAAAGTTTATGTTGCATTTCATGATGAATGCTGGGGAGTTCCAGTATATGATGACAATCAATTGTTTGAGCTGCTTGCCCTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTATTCAGGGAAGCCTTTTCTGGATTTGATCCAAACACAGTTGCCAAAATGGGGGAGGAGGAGATTGAAGAGATAGCTTCCAACAAAGCTTTAATGTTGCCAGACTGCAAAGTCAGGTGCATTGTAGACAATGCCAAATGCATATTGAAGATTGTGAGGGAATGTGGATCTTTCAGTAGCTATATGTGGGGTTCTGTGAATCACAAGCCAGTGATCAACAGGTTCAGACACCCGAGAAATGTTCCTCTGAGGAGCCCGAAAGCAGAAGCCATGAGCAAGGATTTGATAAAGCGGGGATTTCGATACGTTGGACCAGTCATTGTGTACTCATTCATGCAGGCTGCTGGGTTGACCATCGATCATCTTGTGGATTGCTATAGGTATAGTGAGTGTGTGAGCCTTGCAGAAAGACCTTGGAGACATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

371

Amino Acids

42.58

Weight (kDa)

8.94

Isoelectric Point (pI)

59.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 182 - 356 2e-65 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AasI GACNNNNNNGTC 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 813
AciI CCGC 4 cut(s) 424, 437, 440, 979
AclWI GGATC 2 cut(s) 710, 862
AcsI RAATTY 1 cut(s) 59
AfaI GTAC 3 cut(s) 192, 229, 1013
AfiI CCNNNNNNNGG 1 cut(s) 738
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 755, 835
AhlI ACTAGT 1 cut(s) 233
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 8 cut(s) 136, 246, 495, 625, 688, 764, 776, 867
AluI AGCT 8 cut(s) 136, 246, 495, 625, 688, 764, 776, 867
Alw26I GTCTC 3 cut(s) 122, 462, 1099
AlwI GGATC 2 cut(s) 710, 862
Ama87I CYCGRG 1 cut(s) 919
ApeKI GCWGC 2 cut(s) 625, 1028
ApoI RAATTY 1 cut(s) 59
AspS9I GGNCC 2 cut(s) 652, 998
AsuHPI GGTGA 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 919
AvaII GGWCC 2 cut(s) 652, 998
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 944
BbsI GAAGAC 1 cut(s) 187
BbvI GCAGC 2 cut(s) 612, 1015
BccI CCATC 2 cut(s) 643, 1049
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 900
BcoDI GTCTC 3 cut(s) 122, 462, 1099
BcuI ACTAGT 1 cut(s) 233
BfaI CTAG 2 cut(s) 234, 1114
BfmI CTRYAG 1 cut(s) 1063
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 3 cut(s) 440, 626, 1029
BlsI GCNGC 3 cut(s) 441, 627, 1030
BmcAI AGTACT 2 cut(s) 192, 229
Bme1390I CCNGG 1 cut(s) 500
Bme18I GGWCC 2 cut(s) 652, 998
BmeT110I CYCGRG 1 cut(s) 919
BmgT120I GGNCC 2 cut(s) 652, 998
BmiI GGNNCC 3 cut(s) 213, 465, 941
BmrFI CCNGG 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 187
BpmI CTGGAG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 1044
BsaBI GATNNNNATC 1 cut(s) 609
BsaJI CCNNGG 2 cut(s) 216, 1100
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 1 cut(s) 738
Bse1I ACTGG 3 cut(s) 596, 896, 1001
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 1 cut(s) 500
BseCI ATCGAT 1 cut(s) 1044
BseDI CCNNGG 2 cut(s) 216, 1100
BseGI GGATG 1 cut(s) 329
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 1 cut(s) 738
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 926
BseNI ACTGG 3 cut(s) 596, 896, 1001
BseRI GAGGAG 4 cut(s) 538, 758, 761, 952
BseXI GCAGC 2 cut(s) 612, 1015
BseYI CCCAGC 2 cut(s) 585, 1031
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 1044
BsiHKCI CYCGRG 1 cut(s) 919
BsiSI CCGG 1 cut(s) 461
BslI CCNNNNNNNGG 1 cut(s) 738
BsmAI GTCTC 3 cut(s) 122, 462, 1099
BsmI GAATGC 1 cut(s) 587
BsoBI CYCGRG 1 cut(s) 919
Bsp1286I GDGCHC 1 cut(s) 944
Bsp143I GATC 5 cut(s) 400, 715, 854, 900, 1045
BspACI CCGC 4 cut(s) 424, 437, 440, 979
BspCNI CTCAG 1 cut(s) 927
BspDI ATCGAT 1 cut(s) 1044
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 213, 465, 941
BspPI GGATC 2 cut(s) 710, 862
BspT107I GGYRCC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 3 cut(s) 596, 896, 1001
BssECI CCNNGG 2 cut(s) 216, 1100
BssMI GATC 5 cut(s) 400, 715, 854, 900, 1045
BssT1I CCWWGG 2 cut(s) 216, 1100
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 727
Bst6I CTCTTC 4 cut(s) 218, 373, 445, 750
BstC8I GCNNGC 2 cut(s) 630, 1026
BstDEI CTNAG 1 cut(s) 935
BstF5I GGATG 1 cut(s) 329
BstKTI GATC 5 cut(s) 403, 718, 857, 903, 1048
BstMAI GTCTC 3 cut(s) 122, 462, 1099
BstMBI GATC 5 cut(s) 400, 715, 854, 900, 1045
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 498
BstSFI CTRYAG 1 cut(s) 1063
BstV1I GCAGC 2 cut(s) 612, 1015
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 854
BstYI RGATCY 1 cut(s) 854
Bsu15I ATCGAT 1 cut(s) 1044
BsuI GTATCC 1 cut(s) 496
BsuTUI ATCGAT 1 cut(s) 1044
BtsCI GGATG 1 cut(s) 329
BtsIMutI CAGTG 1 cut(s) 903
Cac8I GCNNGC 2 cut(s) 630, 1026
CciI TCATGA 1 cut(s) 574
Cfr13I GGNCC 2 cut(s) 652, 998
ClaI ATCGAT 1 cut(s) 1044
Csp6I GTAC 3 cut(s) 191, 228, 1012
CviAII CATG 5 cut(s) 5, 160, 575, 958, 1021
CviQI GTAC 3 cut(s) 191, 228, 1012
DdeI CTNAG 1 cut(s) 935
DpnI GATC 5 cut(s) 402, 717, 856, 902, 1047
DpnII GATC 5 cut(s) 400, 715, 854, 900, 1045
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
Eam1104I CTCTTC 4 cut(s) 218, 373, 445, 750
EarI CTCTTC 4 cut(s) 218, 373, 445, 750
EciI GGCGGA 1 cut(s) 413
Eco130I CCWWGG 2 cut(s) 216, 1100
Eco24I GRGCYC 1 cut(s) 944
Eco47I GGWCC 2 cut(s) 652, 998
Eco88I CYCGRG 1 cut(s) 919
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 2 cut(s) 216, 1100
EcoT22I ATGCAT 2 cut(s) 6, 830
EcoT38I GRGCYC 1 cut(s) 944
ErhI CCWWGG 2 cut(s) 216, 1100
FaeI CATG 5 cut(s) 8, 163, 578, 961, 1024
FatI CATG 5 cut(s) 4, 159, 574, 957, 1020
FauI CCCGC 1 cut(s) 972
FbaI TGATCA 1 cut(s) 900
FblI GTMKAC 1 cut(s) 813
Fnu4HI GCNGC 3 cut(s) 440, 626, 1029
FokI GGATG 1 cut(s) 316
FriOI GRGCYC 1 cut(s) 944
Fsp4HI GCNGC 3 cut(s) 440, 626, 1029
FspBI CTAG 2 cut(s) 234, 1114
GluI GCNGC 3 cut(s) 440, 626, 1029
GsaI CCCAGC 2 cut(s) 589, 1035
GsuI CTGGAG 1 cut(s) 218
HapII CCGG 1 cut(s) 461
Hin1II CATG 5 cut(s) 8, 163, 578, 961, 1024
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 6 cut(s) 276, 365, 383, 455, 487, 886
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 12
Hpy166II GTNNAC 3 cut(s) 814, 1012, 1038
Hpy188I TCNGA 5 cut(s) 352, 405, 492, 914, 936
Hpy188III TCNNGA 3 cut(s) 197, 575, 708
Hpy8I GTNNAC 3 cut(s) 814, 1012, 1038
HpyAV CCTTC 1 cut(s) 675
HpyCH4III ACNGT 1 cut(s) 727
HpyCH4IV ACGT 1 cut(s) 993
HpyF3I CTNAG 1 cut(s) 935
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 5 cut(s) 8, 163, 578, 961, 1024
Ksp22I TGATCA 1 cut(s) 900
Kzo9I GATC 5 cut(s) 400, 715, 854, 900, 1045
LmnI GCTCC 3 cut(s) 251, 685, 939
Lsp1109I GCAGC 2 cut(s) 612, 1015
MaeI CTAG 2 cut(s) 234, 1114
MaeII ACGT 1 cut(s) 993
MalI GATC 5 cut(s) 402, 717, 856, 902, 1047
MboI GATC 5 cut(s) 400, 715, 854, 900, 1045
MfeI CAATTG 4 cut(s) 285, 532, 614, 658
MflI RGATCY 1 cut(s) 854
MhlI GDGCHC 1 cut(s) 944
MluCI AATT 8 cut(s) 59, 64, 185, 285, 532, 614, 658, 669
MlyI GAGTC 2 cut(s) 359, 464
MmeI TCCRAC 3 cut(s) 502, 792, 976
Mph1103I ATGCAT 2 cut(s) 6, 830
MseI TTAA 4 cut(s) 63, 102, 120, 779
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 500
MunI CAATTG 4 cut(s) 285, 532, 614, 658
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 1 cut(s) 500
NdeII GATC 5 cut(s) 400, 715, 854, 900, 1045
NlaIII CATG 5 cut(s) 8, 163, 578, 961, 1024
NlaIV GGNNCC 3 cut(s) 213, 465, 941
NsiI ATGCAT 2 cut(s) 6, 830
NspI RCATGY 1 cut(s) 163
PagI TCATGA 1 cut(s) 574
PciI ACATGT 1 cut(s) 159
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 4 cut(s) 276, 383, 487, 886
PfoI TCCNGGA 1 cut(s) 498
PkrI GCNGC 3 cut(s) 441, 627, 1030
PleI GAGTC 2 cut(s) 359, 463
PpsI GAGTC 2 cut(s) 359, 463
PscI ACATGT 1 cut(s) 159
PsiI TTATAA 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 2 cut(s) 585, 1031
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 3 cut(s) 213, 465, 941
PspPI GGNCC 2 cut(s) 652, 998
PsuI RGATCY 1 cut(s) 854
RsaI GTAC 3 cut(s) 192, 229, 1013
RsaNI GTAC 3 cut(s) 191, 228, 1012
SaqAI TTAA 4 cut(s) 63, 102, 120, 779
SatI GCNGC 3 cut(s) 440, 626, 1029
Sau3AI GATC 5 cut(s) 400, 715, 854, 900, 1045
Sau96I GGNCC 2 cut(s) 652, 998
ScaI AGTACT 2 cut(s) 192, 229
SchI GAGTC 2 cut(s) 359, 464
ScrFI CCNGG 1 cut(s) 500
SduI GDGCHC 1 cut(s) 944
SfcI CTRYAG 1 cut(s) 1063
SinI GGWCC 2 cut(s) 652, 998
SpeI ACTAGT 1 cut(s) 233
Sse9I AATT 8 cut(s) 59, 64, 185, 285, 532, 614, 658, 669
SsiI CCGC 4 cut(s) 424, 437, 440, 979
SspMI CTAG 2 cut(s) 234, 1114
StyD4I CCNGG 1 cut(s) 498
StyI CCWWGG 2 cut(s) 216, 1100
TaaI ACNGT 1 cut(s) 727
TaiI ACGT 1 cut(s) 996
TaqI TCGA 3 cut(s) 399, 988, 1044
TasI AATT 8 cut(s) 59, 64, 185, 285, 532, 614, 658, 669
TatI WGTACW 3 cut(s) 190, 227, 1011
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 4 cut(s) 276, 383, 487, 886
Tru1I TTAA 4 cut(s) 63, 102, 120, 779
Tru9I TTAA 4 cut(s) 63, 102, 120, 779
TscAI CASTG 1 cut(s) 903
TseI GCWGC 2 cut(s) 625, 1028
TspDTI ATGAA 7 cut(s) 21, 128, 297, 402, 563, 594, 1009
TspRI CASTG 1 cut(s) 903
VpaK11BI GGWCC 2 cut(s) 652, 998
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 2 cut(s) 234, 1114
ZrmI AGTACT 2 cut(s) 192, 229
Zsp2I ATGCAT 2 cut(s) 6, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.