RchiOBHm_Chr4g0437191

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007725
Physical Location & Seq
Forward (+)
60359522 .. 60361056
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40545

Sequence Viewer

Length: 540 bp
ATGTCTAAAGCAAATGTAAGAAGACAATTAGTACTTCTGGAGAAGAACAAGGTTCCCAAGGAGAAGAGTACTACTAGTGCTTTCAGCTCCCACTTTTCTTATAAACACCTAAAGAGAATCTACCCAATTGGGCTTCACAAGAGTTCTTCATCTTCCTCTCTATCTCTATCATCCTTATCCTTGTCTTTGTCAGAAAACTCCATTGACTCTTCTTCTATCATTGATTCTACTTCTCCACTCGATCAGAAGATTTCATCAGCACTCCGCCTCATTGCACCACCGCCCCAAAGAAGAGAGTCTCCGGTGCCTAAAGTTGTCCAACAGCAGAATCAGAGCTTCCAGGATACTGATAATGGGGAGTTGAGGAGGTGCAATTGGATAACAAAGAACAGCGATAAAGTTTATGTTGCATTTCATGATGAATGCTGGGGAGTTCCAGTATATGATGACAATCAATTGTTTGAGCTGCTTGCCCTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTATTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

179

Amino Acids

20.39

Weight (kDa)

9.27

Isoelectric Point (pI)

68.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 129 - 179 5e-15 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 304
AciI CCGC 2 cut(s) 265, 281
AfaI GTAC 2 cut(s) 33, 70
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 4 cut(s) 87, 336, 466, 529
AluI AGCT 4 cut(s) 87, 336, 466, 529
Alw26I GTCTC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 493
AvaII GGWCC 1 cut(s) 493
BanI GGYRCC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 453
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 1 cut(s) 341
BciVI GTATCC 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 303
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 1 cut(s) 75
BfuI GTATCC 1 cut(s) 337
BisI GCNGC 1 cut(s) 467
BlsI GCNGC 1 cut(s) 468
BmcAI AGTACT 2 cut(s) 33, 70
Bme1390I CCNGG 1 cut(s) 341
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 2 cut(s) 54, 306
BmrFI CCNGG 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 59
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 270
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 450
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 379
BseXI GCAGC 1 cut(s) 453
BseYI CCCAGC 1 cut(s) 426
BshNI GGYRCC 1 cut(s) 304
BsiSI CCGG 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 428
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 2 cut(s) 265, 281
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 2 cut(s) 54, 306
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 270
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 341
Bst6I CTCTTC 3 cut(s) 59, 214, 286
BstC8I GCNNGC 1 cut(s) 471
BstF5I GGATG 1 cut(s) 170
BstKTI GATC 1 cut(s) 244
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 1 cut(s) 241
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 339
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 1 cut(s) 28
BsuI GTATCC 1 cut(s) 337
BtsCI GGATG 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 471
CciI TCATGA 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 493
Csp6I GTAC 2 cut(s) 32, 69
CviAII CATG 1 cut(s) 416
CviJI RGCY 5 cut(s) 87, 133, 336, 466, 529
CviKI_1 RGCY 5 cut(s) 87, 133, 336, 466, 529
CviQI GTAC 2 cut(s) 32, 69
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 59, 214, 286
EarI CTCTTC 3 cut(s) 59, 214, 286
EciI GGCGGA 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 1 cut(s) 419
FaiI YATR 6 cut(s) 102, 405, 417, 442, 444, 485
FatI CATG 1 cut(s) 415
Fnu4HI GCNGC 1 cut(s) 467
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 467
FspBI CTAG 1 cut(s) 75
GluI GCNGC 1 cut(s) 467
GsaI CCCAGC 1 cut(s) 430
GsuI CTGGAG 1 cut(s) 59
HapII CCGG 1 cut(s) 302
Hin1II CATG 1 cut(s) 419
HinfI GANTC 5 cut(s) 117, 206, 224, 296, 328
HpaII CCGG 1 cut(s) 302
Hpy188I TCNGA 3 cut(s) 193, 246, 333
Hpy188III TCNNGA 2 cut(s) 38, 416
HpyAV CCTTC 1 cut(s) 516
HpyCH4V TGCA 3 cut(s) 275, 372, 410
Hsp92II CATG 1 cut(s) 419
Kzo9I GATC 1 cut(s) 241
LmnI GCTCC 2 cut(s) 92, 526
LpnPI CCDG 9 cut(s) 23, 315, 326, 353, 412, 450, 465, 488, 520
Lsp1109I GCAGC 1 cut(s) 453
MaeI CTAG 1 cut(s) 75
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 9 cut(s) 33, 55, 76, 138, 144, 201, 204, 259, 303
MfeI CAATTG 4 cut(s) 126, 373, 455, 499
MluCI AATT 6 cut(s) 26, 126, 373, 455, 499, 510
MlyI GAGTC 2 cut(s) 200, 305
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 4 cut(s) 166, 278, 357, 360
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 341
MunI CAATTG 4 cut(s) 126, 373, 455, 499
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 1 cut(s) 341
NdeII GATC 1 cut(s) 241
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 2 cut(s) 54, 306
PagI TCATGA 1 cut(s) 415
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 3 cut(s) 117, 224, 328
PfoI TCCNGGA 1 cut(s) 339
PkrI GCNGC 1 cut(s) 468
PleI GAGTC 2 cut(s) 200, 304
PpsI GAGTC 2 cut(s) 200, 304
PsiI TTATAA 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 426
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 54, 306
PspPI GGNCC 1 cut(s) 493
RsaI GTAC 2 cut(s) 33, 70
RsaNI GTAC 2 cut(s) 32, 69
SatI GCNGC 1 cut(s) 467
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 1 cut(s) 493
ScaI AGTACT 2 cut(s) 33, 70
SchI GAGTC 2 cut(s) 200, 305
ScrFI CCNGG 1 cut(s) 341
SetI ASST 8 cut(s) 54, 89, 111, 338, 371, 468, 531, 539
SinI GGWCC 1 cut(s) 493
SpeI ACTAGT 1 cut(s) 74
Sse9I AATT 6 cut(s) 26, 126, 373, 455, 499, 510
SsiI CCGC 2 cut(s) 265, 281
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 339
StyI CCWWGG 1 cut(s) 57
TaqI TCGA 1 cut(s) 240
TasI AATT 6 cut(s) 26, 126, 373, 455, 499, 510
TatI WGTACW 2 cut(s) 31, 68
TfiI GAWTC 3 cut(s) 117, 224, 328
TseI GCWGC 1 cut(s) 466
TspDTI ATGAA 4 cut(s) 138, 243, 404, 435
VpaK11BI GGWCC 1 cut(s) 493
XspI CTAG 1 cut(s) 75
ZrmI AGTACT 2 cut(s) 33, 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.