pycom13g04930

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007725
Physical Location & Seq
Forward (+)
3300151 .. 3302534
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04930.1

Sequence Viewer

Length: 942 bp
ATGTCTGAAGCAAATATTGTAAGAAGACATGTAATTCTGGAGGAGAACAAAGTTGTAAAAGAGAGAGAGAAGACGAACAGTAGCTCTAAACTCCTCAAGAGAATTTACCCAATTGGCCTTCACAAGAGCACTTCATCTCTATCCCTATCCTCATCTTTGTCATACTCTATGTCGGAGAACTCGTATGATTATTTTCTCACTGATTCTAACTTCCCACTGGATCAGAAGATTTCCGCGGCTTTCCGGTTCTTTGCGCAACCACCTCCAAGAAGAGAGTATAATTCTCCAGTGGCTAAACTGGCCCAGCAGCAGATTAGTCTGGCTCAGGATGCTAATGATGGGGAGTTGAGGAGATGCAATTGGATAACAAAGAATAGTGATAAAGTTTATGTAGTGTTTCACGACGAATGCTGGGGAGTTCCAGCATACGATGACAATCAATTGTTTGAGCTGCTTGCACTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTTTTCAGGGAATCGTTTTCTGGATTTGATCCGAACAAAGTTGCGAAAATGGGGGAGAAAGAGATTGCAGAAATAACCTCCAACAAAGAAATAATGCTGGTAGACTGCAAAGTGAGGTGCATTATAGACAATGCCAAATGCATATTGAAGATTGTAAGGGAGTTTGGATCTTTCAGCAGCTACGTGTGGAGTTCTGTGAATCACAAACCGGTCATAAACCGATTCAGATACCCGAGAAACGTTCCTCTGAGGACCCCGAAAGCAGAATCCATGAGCAAGGATTTGATCAAGAGAGGATTTCGGTACGTTGGACCGGTGATTGTGTACTCGTTCATGCAGGCTGCAGGGCTGACAAATGATCATCTGGTGGATTGCTATAGGTACAGTGAATGTGTAAACCTTGCAGAAAGACCTTGGAGACATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

314

Amino Acids

36.25

Weight (kDa)

9.03

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 124 - 298 4.9e-67 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 255
AccI GTMKAC 1 cut(s) 615
AccII CGCG 1 cut(s) 236
AciI CCGC 2 cut(s) 234, 236
AclI AACGTT 1 cut(s) 753
AclWI GGATC 3 cut(s) 228, 536, 688
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 3 cut(s) 818, 839, 896
AflIII ACRYGT 2 cut(s) 28, 696
AgeI ACCGGT 2 cut(s) 721, 826
AgsI TTSAA 1 cut(s) 661
AluBI AGCT 4 cut(s) 84, 451, 514, 693
AluI AGCT 4 cut(s) 84, 451, 514, 693
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 925
AlwI GGATC 3 cut(s) 228, 536, 688
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 2 cut(s) 115, 300
ApeKI GCWGC 4 cut(s) 307, 451, 690, 854
ApoI RAATTY 1 cut(s) 102
AsiGI ACCGGT 2 cut(s) 721, 826
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 4 cut(s) 301, 478, 765, 824
AsuHPI GGTGA 1 cut(s) 841
AvaI CYCGRG 1 cut(s) 745
AvaII GGWCC 3 cut(s) 478, 765, 824
BbsI GAAGAC 2 cut(s) 31, 77
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 4 cut(s) 319, 438, 702, 841
BccI CCATC 2 cut(s) 332, 469
BclI TGATCA 2 cut(s) 798, 871
BcoDI GTCTC 1 cut(s) 925
BfmI CTRYAG 2 cut(s) 855, 889
BisI GCNGC 5 cut(s) 237, 308, 452, 691, 855
BlsI GCNGC 5 cut(s) 238, 309, 453, 692, 856
Bme18I GGWCC 3 cut(s) 478, 765, 824
BmeT110I CYCGRG 1 cut(s) 745
BmgT120I GGNCC 4 cut(s) 301, 478, 765, 824
BmiI GGNNCC 1 cut(s) 767
BmsI GCATC 2 cut(s) 319, 344
BpiI GAAGAC 2 cut(s) 31, 77
BpmI CTGGAG 2 cut(s) 59, 270
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 80
BsaAI YACGTR 1 cut(s) 697
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 234, 926
BsaWI WCCGGW 3 cut(s) 243, 721, 826
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bse118I RCCGGY 2 cut(s) 721, 826
Bse1I ACTGG 3 cut(s) 222, 287, 303
Bse8I GATNNNNATC 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 234, 926
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 2 cut(s) 338, 752
BseNI ACTGG 3 cut(s) 222, 287, 303
BseRI GAGGAG 3 cut(s) 56, 83, 364
BseXI GCAGC 4 cut(s) 319, 438, 702, 841
BseYI CCCAGC 2 cut(s) 303, 411
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 117, 302
BshTI ACCGGT 2 cut(s) 721, 826
BsiHKAI GWGCWC 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 3 cut(s) 244, 722, 827
BsmAI GTCTC 1 cut(s) 925
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 2 cut(s) 117, 302
BsoBI CYCGRG 1 cut(s) 745
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 5 cut(s) 220, 541, 680, 798, 871
BspACI CCGC 2 cut(s) 234, 236
BspANI GGCC 2 cut(s) 117, 302
BspCNI CTCAG 2 cut(s) 337, 753
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 1 cut(s) 767
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 3 cut(s) 228, 536, 688
BsrFI RCCGGY 2 cut(s) 721, 826
BsrI ACTGG 3 cut(s) 222, 287, 303
BssAI RCCGGY 2 cut(s) 721, 826
BssECI CCNNGG 2 cut(s) 234, 926
BssMI GATC 5 cut(s) 220, 541, 680, 798, 871
BssT1I CCWWGG 1 cut(s) 926
Bst4CI ACNGT 3 cut(s) 80, 462, 899
Bst6I CTCTTC 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 697
BstC8I GCNNGC 2 cut(s) 456, 852
BstDEI CTNAG 2 cut(s) 324, 761
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 236
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 5 cut(s) 223, 544, 683, 801, 874
BstMAI GTCTC 1 cut(s) 925
BstMBI GATC 5 cut(s) 220, 541, 680, 798, 871
BstMWI GCNNNNNNNGC 2 cut(s) 299, 329
BstNSI RCATGY 1 cut(s) 32
BstSFI CTRYAG 2 cut(s) 855, 889
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 4 cut(s) 319, 438, 702, 841
BstV2I GAAGAC 2 cut(s) 31, 77
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 2 cut(s) 117, 302
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 5 cut(s) 198, 215, 294, 458, 904
Cac8I GCNNGC 2 cut(s) 456, 852
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 2 cut(s) 721, 826
Cfr13I GGNCC 4 cut(s) 301, 478, 765, 824
Cfr42I CCGCGG 1 cut(s) 237
Csp6I GTAC 3 cut(s) 817, 838, 895
CspAI ACCGGT 2 cut(s) 721, 826
CviAII CATG 3 cut(s) 29, 784, 847
CviQI GTAC 3 cut(s) 817, 838, 895
DdeI CTNAG 2 cut(s) 324, 761
DpnI GATC 5 cut(s) 222, 543, 682, 800, 873
DpnII GATC 5 cut(s) 220, 541, 680, 798, 871
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 926
Eco47I GGWCC 3 cut(s) 478, 765, 824
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 745
EcoO109I RGGNCCY 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 926
EcoT22I ATGCAT 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 926
FaeI CATG 3 cut(s) 32, 787, 850
FatI CATG 3 cut(s) 28, 783, 846
FbaI TGATCA 2 cut(s) 798, 871
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 5 cut(s) 237, 308, 452, 691, 855
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 5 cut(s) 237, 308, 452, 691, 855
FspI TGCGCA 1 cut(s) 255
GlaI GCGC 1 cut(s) 255
GluI GCNGC 5 cut(s) 237, 308, 452, 691, 855
GsaI CCCAGC 2 cut(s) 307, 415
GsuI CTGGAG 2 cut(s) 59, 270
HaeIII GGCC 2 cut(s) 117, 302
HapII CCGG 3 cut(s) 244, 722, 827
HhaI GCGC 1 cut(s) 256
Hin1II CATG 3 cut(s) 32, 787, 850
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HinfI GANTC 5 cut(s) 203, 524, 712, 735, 779
HpaII CCGG 3 cut(s) 244, 722, 827
HphI GGTGA 1 cut(s) 841
Hpy166II GTNNAC 3 cut(s) 616, 838, 910
Hpy188I TCNGA 6 cut(s) 7, 175, 225, 546, 740, 762
Hpy188III TCNNGA 6 cut(s) 38, 97, 326, 401, 534, 802
Hpy8I GTNNAC 3 cut(s) 616, 838, 910
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 2 cut(s) 128, 501
HpyCH4III ACNGT 3 cut(s) 80, 462, 899
HpyCH4IV ACGT 3 cut(s) 696, 753, 819
HpyCH4V TGCA 9 cut(s) 357, 458, 581, 621, 633, 654, 850, 857, 917
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 329
HpyF3I CTNAG 2 cut(s) 324, 761
HpySE526I ACGT 3 cut(s) 696, 753, 819
Hsp92II CATG 3 cut(s) 32, 787, 850
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 2 cut(s) 798, 871
KspI CCGCGG 1 cut(s) 237
Kzo9I GATC 5 cut(s) 220, 541, 680, 798, 871
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 4 cut(s) 319, 438, 702, 841
LweI GCATC 2 cut(s) 319, 344
MaeII ACGT 3 cut(s) 696, 753, 819
MalI GATC 5 cut(s) 222, 543, 682, 800, 873
MboI GATC 5 cut(s) 220, 541, 680, 798, 871
MboII GAAGA 5 cut(s) 36, 82, 238, 282, 673
MfeI CAATTG 4 cut(s) 111, 358, 440, 484
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 8 cut(s) 33, 102, 111, 280, 358, 440, 484, 495
MmeI TCCRAC 3 cut(s) 153, 618, 802
Mph1103I ATGCAT 1 cut(s) 656
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 244, 722, 827
MunI CAATTG 4 cut(s) 111, 358, 440, 484
Mva1269I GAATGC 1 cut(s) 413
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 299, 329
NdeII GATC 5 cut(s) 220, 541, 680, 798, 871
NlaIII CATG 3 cut(s) 32, 787, 850
NlaIV GGNNCC 1 cut(s) 767
NsbI TGCGCA 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 656
NspI RCATGY 1 cut(s) 32
PciI ACATGT 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 5 cut(s) 203, 524, 712, 735, 779
PinAI ACCGGT 2 cut(s) 721, 826
PkrI GCNGC 5 cut(s) 238, 309, 453, 692, 856
Ppu21I YACGTR 1 cut(s) 697
PpuMI RGGWCCY 1 cut(s) 765
PscI ACATGT 1 cut(s) 28
Psp1406I AACGTT 1 cut(s) 753
Psp5II RGGWCCY 1 cut(s) 765
PspFI CCCAGC 2 cut(s) 303, 411
PspN4I GGNNCC 1 cut(s) 767
PspPI GGNCC 4 cut(s) 301, 478, 765, 824
PspPPI RGGWCCY 1 cut(s) 765
PstI CTGCAG 1 cut(s) 859
PsuI RGATCY 1 cut(s) 680
RsaI GTAC 3 cut(s) 818, 839, 896
RsaNI GTAC 3 cut(s) 817, 838, 895
SacII CCGCGG 1 cut(s) 237
SatI GCNGC 5 cut(s) 237, 308, 452, 691, 855
Sau3AI GATC 5 cut(s) 220, 541, 680, 798, 871
Sau96I GGNCC 4 cut(s) 301, 478, 765, 824
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 2 cut(s) 319, 344
SfcI CTRYAG 2 cut(s) 855, 889
Sfr303I CCGCGG 1 cut(s) 237
SgrBI CCGCGG 1 cut(s) 237
SinI GGWCC 3 cut(s) 478, 765, 824
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 8 cut(s) 33, 102, 111, 280, 358, 440, 484, 495
SsiI CCGC 2 cut(s) 234, 236
SspI AATATT 1 cut(s) 16
StyI CCWWGG 1 cut(s) 926
TaaI ACNGT 3 cut(s) 80, 462, 899
TaiI ACGT 3 cut(s) 699, 756, 822
TasI AATT 8 cut(s) 33, 102, 111, 280, 358, 440, 484, 495
TatI WGTACW 1 cut(s) 837
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 5 cut(s) 203, 524, 712, 735, 779
TscAI CASTG 5 cut(s) 205, 222, 294, 465, 904
TseI GCWGC 4 cut(s) 307, 451, 690, 854
TspDTI ATGAA 2 cut(s) 123, 835
TspRI CASTG 5 cut(s) 205, 222, 294, 465, 904
VpaK11BI GGWCC 3 cut(s) 478, 765, 824
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 615
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.